Reduction of in-shell Brazil nut (Bertholletia excelsa H.B.K.) aflatoxin contamination by ozone gas application during storage

The susceptibility of the in-shell Brazil nut mycoflora and aflatoxins (AFLs) contamination to ozone (O3) gas during storage is reported. In-shell Brazil nuts obtained from retail market were submitted to O3 gas atmosphere at different concentrations immediately before to be stored. Samples were collected just after the gas exposure and every 30 days during the storage period to carry on mycological tests and AFLs analysis. A sensorial evaluation by descriptive quality analysis was carried out to check treated nuts sensory attributes according to consumer acceptance after gas exposure. The O3 treatment applied within 5 h at 31 mg/L was able to successfully inhibit the viability of fungi of the nut-contaminating microflora and so the toxigenic Aspergillus species from the day of application. AFLs were totally degraded in all samples whatever O3 concentration applied. No significant changes on sensory attributes were observed that could affect nut acceptability  after the O3 treatments and storage conditions applied in the present experiment. This procedure is tentatively applied at an Amazon State nut factory for checking its potential in mycotoxin risk contamination of in-shell Brazil nuts safeguarding under the Amazon region environment. Keywords: In-shell Brazil nut, Ozone, Mycoflora, Aflatoxin, Storage, Sensory evaluation

Effect of oxygen reducing atmospheres on the quality and safety of stored shelled Brazil nut packs

High moisture content, relative humidity, temperature and environment rich in oxygen (O2) are the main factors for tree nuts to get infected by fungi and so aflatoxins (AFLs) contaminated. During storage and commercialization dry Brazil nuts packs need to maintain their safety and quality. Modified atmospheres in storage (macro-environment) and packaging (micro-environment) have been used to prolong food shelf life by reducing O2 concentration with inhibitory gases or, more recently, by adding O2 absorber pads. This work reports the application of O2 atmosphere reducing methods on stored shelled Brazil nut packs aiming fungi and AFL degradation as well as hygienic conditions improvements. The methods applied were: (a) ozone - O3, (b) carbon dioxide - CO2 and (c) O2 absorber pads with and without vacuum. Nuts were submitted to microbiological tests (fungi, aflatoxigenic strains, yeast and bacteria), moisture content and AFLs analysis. From all O2 reducing atmosphere evaluated, the best performance was obtained with O3. A reduction on fungi growth (1.8 x 104 cfu.g-1 to 2.6 x 10 cfu.g-1) and yeast destruction after the first month of storage were registered. Also O3 was the only nut treatment that was able to degrade AFLs. None of the spiked (AFLs: 15 ppb) nut samples O3 treated had AFLs detected up to the LOQ of the method (0.36 μg.kg-1 for AFB1+AFB2+AFG1+AFG2) i.e., much lower than the allowed by the European Union regulation (MRL: 4 and 2 ppb for total and AFB1, respectively), thus producing safer nuts. All other treatments stabilized and/or inhibited microorganisms growth. Add CO2 and O2 pads played an important role on nut quality. Further study will be carried out in order to adjust O3 concentration and application conditions for longer period of storage

Monitoring System for Agrometeorological Application with Voice-Controlled Interface

The objective of this work is to present aspects about the already completed development stages of a monitoring system for agrometeorological application that uses Human-Computer Interface controlled by written and spoken languages. Technologies related to the development of this type of HCI have been increasingly used and are gradually more connected to the most diverse devices and machines including fieldwork uses. This interdisciplinary work is supported by research in the areas of Meteorology, Linguistics, Natural Language Processing (NPL) and Computing using physical prototypes focused on monitoring: automated solar search, unmanned aerial vehicle (UAV), unmanned groundvehicle (UGV), mix of meteorological sensors and the system itself. The steps already completed and interrelated - automated solar tracker, the set of meteorological sensors and the system - show that this type of monitoring has a significant degree of accuracy, low cost and autonomy - it does not depend on the conventional grid and makes small decisions

Asymptomatic HIV-people present different profile of sCD14, sRAGE, DNA damage and vitamins, according to the use of cART and CD4+ T cells restoration.

We aimed to analyze markers of immune activation, inflammation, and oxidative stress in 92 asymptomatic HIV-infected patients according to the adequate (AR, >500 cells/mm(3)) or inadequate (IR, nAR > LTNP > tAR > tIR. Higher levels of 8-isoprostane were observed in nIR. LTNP presented a higher retinol dosage than tAR and less genotoxic damage induced by oxidative stress than the other groups. We suggest that the therapy, despite being related to lesser immune activation and inflammation, alters the vitamin profile and consequently increases the oxidative stress of patients. In addition, the lowest genotoxic index for LTNP indicates that both VL and cART could be responsible for the increased DNA damage. More studies are needed to understand the influence of cART on persistent immune activation and inflammation

Stability and sensory assessment of emulsions containing propolis extract and/or tocopheryl acetate

The prevention of skin aging has been one of the main aims of cosmetic products. Propolis and tocopheryl acetate can be promising substances because of their antioxidant properties. In this study, propolis extract was obtained and associated with tocopheryl acetate in a cream formulation, which then underwent stability and sensory assessment. The formulation containing propolis extract and tocopheryl acetate proved to be stable in the preliminary stability study, demonstrating gradual darkening and slight pH decrease when subjected to 60ºC for 28 days, but showing stability on rheological study. In the sensory analysis, the formulation containing these two components was preferred by the product testers over the base cream and creams containing propolis extract or tocopheryl acetate alone. In conclusion, given the stability of the formulation and the preference of the product testers for this formulation, this association proved promising for use in cosmetic formulations.A prevenção do envelhecimento cutâneo tem sido um dos principais focos dos produtos dermocosméticos. A própolis contém substâncias com atividade antioxidante, bem como o acetato de tocoferila é conhecido por apresentar esta atividade. Porém, a própolis apresenta odor muito característico e intenso, que pode interferir no sensorial do produto. Assim, no presente trabalho, obteve-se o extrato de própolis, que foi associado ao acetato de tocoferila em uma formulação de uso tópico, que foi avaliada quanto à estabilidade e às características sensoriais. Conduziu-se um estudo de estabilidade, no qual as formulações contendo ambos os compostos apresentaram escurecimento gradual e ligeira queda no pH após 28 dias sob 60 °C, tendo sido estável no estudo reológico. Na análise sensorial, realizada com 28 provadores, a formulação contendo extrato de própolis em associação com acetato de tocoferila foi a preferida, quando comparada com o creme base e o creme contendo somente extrato de própolis ou acetato de tocoferila. Em conclusão, devido à preferência dos provadores e ao estudo de estabilidade, a associação de extrato de própolis e de acetato de tocoferila mostrou ser promissora para uso em produtos dermocosméticos

Reliability intra-and inter-examiner of the head postural assessment by computerized photogrammetry

Scientific articles about reliability of photogrammetry for cervical spine posture evaluation are infrequent. The aim of the present investigation is to verify intra-and inter-examiner reliability of the computerized photogrammetry method for head postural evaluation in lateral view. Twenty-five young women, between 20 and 30 years old, were positioned seated in an upright position and photographed in lateral view. The photographs were imported to Corel Draw X13 program for postural evaluation by computerized photogrammetry. Analyses of intra-and inter-examiner reliability were performed for the angles: condyle-acromion (ACA), menton-sternum (AME) and Frankfurt (AF). The photogrammetry was performed by two examiners: EA and EB. For intra-examiner analyses, EA assessed the pictures twice (A1 and A2) for the same angles within 3 months. For the inter-examiner analyses, EB performed the photogrammetry for the same angles (B1) in order to compare with the data from EA. Using the interclass correlation coefficient (ICC) we observed an excellent correlation in the intra-examiner analysis (A1 and A2) for the angles: ACA and AME (both with ICC=1.0); and AF (ICC=0.78). For the inter-examiner analyses between A1 and B1, it was observed: ACA (ICC=0.24), AME (ICC=0.26) and AF (ICC=0.00). For the comparison between A2 and B1, the ICC values were: 0.23; 0.27 and 0.00, respectively for ACA, AME and AF, classified as weak correlations. In conclusion, the photogrammetry is reliable when performed by the same examiner. The inter-examiner assess showed low reliability, what could have been compromised by the reduced experience of the EB in applying the method

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others