Nitrogen transfer between clover and wheat in an intercropping experiment

A novel approach to the problem of improving nitrogen supply in organic farming is to use intercropping of cereals with a legume to provide nitrogen transfer within a season and/or to following crops. The affects of intercropping were studied in a column experiment using mixtures of winter wheat (Triticum aestivum cv. Claire), with white clover (w.c.) (Trifolium repens cv. Barblanca) and with red clover (r.c.) (Trifolium pratense cv. Britta). The effects of cutting and removal above ground clover material with and without additional soil disturbance were compared to leaving clover plants in situ and intercropped with wheat in a split root design. Wheat and clover plants, as monocultures, were used for the controls. 15N ammonium nitrate solution was applied. The wheat seeds were sown into the column without nitrogen. We found that the cutting treatment produced the highest yield of wheat. Available ammonium-N in the soil was greatest in the clover control treatment for the column with only red clover roots and in the cutting+soil disturbance treatment for the column with only white clover roots. Available nitrate-N was greatest in the soil disturbance treatment in the column with clover and wheat roots for both red and white clover. The cutting treatment produced the highest yield of wheat

Challenges and Opportunities in Industry 4.0 for Mechatronics, Artificial Intelligence and Cybernetics

Industry 4.0 has risen as an integrated digital manufacturing environment, and it has created a novel research perspective that has thrust research to interdisciplinarity and exploitation of ICT advances. This work presents and discusses the main aspects of Industry 4.0 and how intelligence can be embedded in manufacturing to create the smart factory. It briefly describes the main components of Industry 4.0, and it focuses on the security challenges that the fully interconnected ecosystem of Industry 4.0 has to meet and the threats for each component. Preserving security has a crucial role in Industry 4.0, and it is vital for its existence, so the main research directions on how to ensure the confidentiality and integrity of the information shared among the Industry 4.0 components are presented. Another view is in light of the security issues that come as a result of enabling new technologies. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Acknowledgements. We thank Dawn Ernstzen, Division of Physiotherapy, Stellenbosch University, for her contextualisation work for chronic pain management in SA used in the example in this article, and Michelle Galloway for her support in finalising the submission on behalf of the author team. Funding. The authors were funded, partially or in full, by the SAGE project, a 3-year (2014 - 2017) Flagship Grant from the South African Medical Research Council. The Flagship Grant programme was not involved in the conceptualisation or conduct of this study

Assessment of proliferating cell nuclear antigen and its relationship with proinflammatory cytokines and parameters of disease activity in multiple myeloma patients

Multiple myeloma (MM) is a malignant plasma cell disease. Several proinflammatory cytokines produced by malignant plasma cells and bone marrow (BM) stromal cells are involved in the pathogenesis of the disease. We evaluated serum levels of the proinflammatory cytokines Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Interleukin-8 (IL-8), macrophage inflammatory protein-1α (MIP-1α), in MM patients before treatment, and determined its significance in tumor progression. We also analyzed the correlation between measured parameters with proliferating cell nuclear antigen (PCNA). Forty-four MM patients and 20 healthy controls were studied. Serum levels of the proinflammatory cytokines were measured using enzyme-linked immunosorbent assay (ELISA), whereas PCNA value in the BM was determined by immunohistochemistry staining. The mean concentrations of the measured cytokines were significantly different among the three stages of disease, with higher values in advanced disease stage. Furthermore, patients with MM had significantly higher serum levels of the measured cytokines than in controls. A positive correlation was found between IL-6 with IL-1β, IL-8 and MIP-1α. Similarly, IL-8 and MIP-1α were positively correlated with markers of disease activity such as β2 microglobulin and LDH. The proliferation index, determined by PCNA immunostaining, was higher in advanced disease stage. Furthermore PCNA value correlated significantly with β2 microglobulin, LDH and the levels of the measured cytokines. Our results showed that the proliferative activity, as measured with PCNA, increases in parallel with disease stage. The positive correlation between PCNA and other measured mediators supports the involvement of these factors in the biology of myeloma cell growth and can be used as markers of disease activity and as possible therapeutic targets

AF-MSCs fate can be regulated by culture conditions

Human mesenchymal stem cells (hMSCs) represent a population of multipotent adherent cells able to differentiate into many lineages. In our previous studies, we isolated and expanded fetal MSCs from second-trimester amniotic fluid (AF) and characterized them based on their phenotype, pluripotency and proteomic profile. In the present study, we investigated the plasticity of these cells based on their differentiation, dedifferentiation and transdifferentiation potential in vitro. To this end, adipocyte-like cells (AL cells) derived from AF-MSCs can regain, under certain culture conditions, a more primitive phenotype through the process of dedifferentiation. Dedifferentiated AL cells derived from AF-MSCs (DAF-MSCs), gradually lost the expression of adipogenic markers and obtained similar morphology and differentiation potential to AF-MSCs, together with regaining the pluripotency marker expression. Moreover, a comparative proteomic analysis of AF-MSCs, AL cells and DAF-MSCs revealed 31 differentially expressed proteins among the three cell populations. Proteins, such as vimentin, galectin-1 and prohibitin that have a significant role in stem cell regulatory mechanisms, were expressed in higher levels in AF-MSCs and DAF-MSCs compared with AL cells. We next investigated whether AL cells could transdifferentiate into hepatocyte-like cells (HL cells) directly or through a dedifferentiation step. AL cells were cultured in hepatogenic medium and 4 days later they obtained a phenotype similar to AF-MSCs, and were termed as transdifferentiated AF-MSCs (TRAF-MSCs). This finding, together with the increase in pluripotency marker expression, indicated the adaption of a more primitive phenotype before transdifferentiation. Additionally, we observed that AF-, DAF- and TRAF-MSCs displayed similar clonogenic potential, secretome and proteome profile. Considering the easy access to this fetal cell source, the plasticity of AF-MSCs and their potential to dedifferentiate and transdifferentiate, AF may provide a valuable tool for cell therapy and tissue engineering applications

An Evaluation of the Anti-Carcinogenic Response of Major Isothiocyanates in Non-Metastatic and Metastatic Melanoma Cells

Malignant melanoma is one of the most deadly types of solid cancers, a property mainly attributed to its highly aggressive metastatic form. On the other hand, different classes of isothiocy- anates, a class of phytochemicals, present in cruciferous vegetables have been characterized by considerable anti-cancer activity in both in vitro and in vivo experimental models. In the current study, we investigated the anti-cancer response of five isothiocyanates in an in vitro model of melanoma consisting of non-metastatic (A375, B16F-10) and metastatic (VMM1, Hs294T) malignant melanoma as well as non-melanoma epidermoid carcinoma (A431) and non-tumorigenic melanocyte-neighboring keratinocyte (HaCaT) cells. Our aim was to compare different endpoints of cytotoxicity (e.g., reactive oxygen species, intracellular glutathione content, cell cycle growth arrest, apoptosis and necrosis) descriptive of an anti-cancer response between non-metastatic and metastatic melanoma as well as non-melanoma epidermoid carcinoma and non-tumorigenic cells. Our results showed that exposure to isothiocyanates induced an increase in intracellular reactive oxygen species and glutathione contents between non-metastatic and metastatic melanoma cells. The distribution of cell cycle phases followed a similar pattern in a manner where non-metastatic and metastatic melanoma cells appeared to be growth arrested at the G2/M phase while elevated levels of metastatic melanoma cells were shown to be at sub G1 phase, an indicator of necrotic cell death. Finally, metastatic melanoma cells were more sensitive apoptosis and/or necrosis as higher levels were observed compared to non-melanoma epidermoid carcinoma and non-tumorigenic cells. In general, non-mela- noma epidermoid carcinoma and non-tumorigenic cells were more resistant under any experimental exposure condition. Overall, our study provides further evidence for the potential development of isothiocyanates as promising anti-cancer against non-metastic and metastatic melanoma cells, a property specific for these cells and not shared by non-melanoma epidermoid carcinoma or non-tumorigenic melanocyte cells

Synthesis of glutathione analogues and screening as substrates & inhibitors for human glutathione transferase p1‐1

A major detoxification mechanism of the cell involves the glutathione transferase (GST)‐catalyzed formation of glutathione (GSH) conjugates with various xenobiotics Based on the same mechanism, GST overexpression may lead to multidrug resistant phenotypes Therefore, several compounds with inhibitory potency against GSTs have been developed as potential tools fortackling GST-­‐attributed MDR. Several individual compounds and prodrugs have been proposed as GST‐inhibiting substances. In addition, GSH analogues have been considered as specific GST inhibitors, with particular attention been directed towards the synthesis of GSH analogues stable against γ‐glutamyltranspeptidase (γGT) and peptidases, as GST inhibitors

Chemical and Biological Characterization of the Anticancer Potency of \u3ci\u3eSalvia fruticosa\u3c/i\u3e in a Model of Human Malignant Melanoma

Malignant melanoma is one of the most aggressive types of skin cancer with an increasing incidence worldwide. Thus, the development of innovative therapeutic approaches is of great importance. Salvia fruticosa (SF) is known for its anticancer properties and in this context, we aimed to investigate its potential anti-melanoma activity in an in vitro model of human malignant melanoma. Cytotoxicity was assessed through a colorimetric-based sulforhodamine-B (SRB) assay in primary malignant melanoma (A375), non-malignant melanoma epidermoid carcinoma (A431) and non-tumorigenic melanocyte neighbouring keratinocyte (HaCaT) cells. Among eight (8) different fractions of S. fruticosa extracts (SF1-SF8) tested, SF3 was found to possess significant cytotoxic activity against A375 cells, while A431 and HaCaT cells remained relatively resistant or exerted no cytotoxicity, respectively. In addition, the total phenolic (Folin–Ciocalteu assay) and total flavonoid content of SF extracts was estimated, whereas the antioxidant capacity was measured via the inhibition of tert-butyl hydroperoxide-induced lipid peroxidation and protein oxidation levels. Finally, apoptotic cell death was assessed by utilizing a commercially available kit for the activation of caspases - 3, - 8 and - 9. In conclusion, the anti-melanoma properties of SF3 involve the induction of both extrinsic and intrinsic apoptotic pathway(s), as evidenced by the increased activity levels of caspases - 8, and - 9, respectively

Classical multiparty computation using quantum resources

International audienceIn this work, we demonstrate a way to perform classical multiparty computing among parties with limited computational resources. Our method harnesses quantum resources to increase the computational power of the individual parties. We show how a set of clients restricted to linear classical processing are able to jointly compute a nonlinear multivariable function that lies beyond their individual capabilities. The clients are only allowed to perform classical xor gates and single-qubit gates on quantum states. We also examine the type of security that can be achieved in this limited setting. Finally, we provide a proof-of-concept implementation using photonic qubits that allows four clients to compute a specific example of a multiparty function, the pairwise AND

Exploring the X-ray Sky with the XMM-Newton Bright Serendipitous Survey

ABRIDGED. We present "The XMM-Newton Bright Serendipitous Survey", two flux-limited samples with flux limit fx ~7E-14 cgs in the 0.5-4.5 keV (BSS) and 4.5-7.5 keV (HBSS) energy band, respectively. After discussing the survey strategy, we present the basic data on a complete sample of 400 X-ray sources derived from the analysis of 237 XMM-Newton fields. The survey covers an area of 28.10 (25.17 for the HBSS) sq. deg. Up to now ~71% (~90%) of the sources have been spectroscopically identified making the BSS (HBSS) the sample with the highest number of identified XMM-Newton sources published so far. We find that: a) the optical counterpart in the majority (~90%) of cases has a magnitude brighter than the POSS II limit; b) the majority of the objects identified so far are broad line AGN. No obvious trend of the source spectra (as deduced from the Hardness Ratios analysis) as a function of the count rate is seen and the average spectra of the "extragalactic" population corresponds to an X-ray energy spectral index of ~0.8 (~0.64) for the BSS (HBSS) sample. About 13% (40%) of the sources are described by an energy spectral index flatter than that of the CXB. We measure a surface density of optically type 1 and type 2 AGN of 1.63+/-0.25 deg-2 and 0.83+/-0.18$ deg-2, respectively; optically type 2 AGN represent 34+/-9% of the total AGN population. Finally, we find a clear separation, in the hardness ratio diagram and in the (hardness ratio) vs. (X-ray to optical flux ratio) diagram, between "coronal emitting" stars and extragalactic sources.Comment: 42 pages, 7 figures, 8 Tables, Accepted for publication in A&

Cosmological Evolution of a Brane Universe in a Type 0 String Background

We study the cosmological evolution of a D3-brane Universe in a type 0 string background. We follow the brane-universe along the radial coordinate of the background and we calculate the energy density which is induced on the brane because of its motion in the bulk. We find that for some typical values of the parameters and for a particular range of values of the scale factor of the brane-universe, the effective energy density is dominated by a term proportional to $\frac{1}{(loga)^{4}}$ indicating a slow varying inflationary phase. For larger values of the scale factor the effective energy density takes a constant value and the brane-universe enters its usual inflationary period.Comment: 25 pages,1 figure,LaTex file,final version to appear in Phys. Rev.