Waste to want: polymer nanocomposites using nanoclays extracted from oil based drilling mud waste.

Due to the European Union (EU) waste frame work directive (WFD), legislations have been endorsed in EU member states such as UK for the Recycling of wastes with a vision to prevent and reduce landfilling of waste. Spent oil based drilling mud (drilling fluid) is a waste from the Oil and Gas industry with great potentials for recycling after appropriate clean-up and treatment processes. This research is the novel application of nanoclays extracted from spent oil based drilling mud (drilling fluid) clean-up as nanofiller in the manufacture of nanocomposite materials. Research and initial experiments have been undertaken which investigate the suitability of Polyamide 6 (PA6) as potential polymer of interest. SEM and EDAX were used to ascertain morphological and elemental characteristics of the nanofiller. ICPOES has been used to ascertain the metal concentration of the untreated nanofiller to be treated (by oil and heavy metal extraction) before the production of nanocomposite materials. The challenges faced and future works are also discussed

To address emerging infections, we must invest in enduring systems: The kinetics and dynamics of health systems strengthening

Clinical pharmacology uses foundational principles of pharmacokinetics (PK) and pharmacodynamics (PD) to address medication use spanning a continuum from molecules to the masses. In the realm of infectious diseases, PK/PD attributes are considered especially important, because subtherapeutic dosing of antibiotics has been associated with poorer clinical outcomes in patients and increased incidences of drug resistance in populations. In consideration of these PK/PD principles, we will describe the analogous relationship between health systems strengthening, including for educating healthcare providers about emerging infections, and the tenets of therapeutic drug monitoring

Efficacy of total lymphocyte count as a surrogate for CD4 cell counts in HIV-infected adults in the era of higher treatment cutoffs and less funding: a cross-sectional study

Abstract Background: Total Lymphocyte Count (TLC) has been previously found to be a suitable surrogate for CD4 counts in the management of HIV in resource limited settings. With the new treatment cutoff of 500 cells/mm 3 , its performance needs to be further evaluated. Objective: To determine the efficacy of TLC as a surrogate for CD4 counts in a resource-limited African setting. Methods: A retrospective, cross-sectional study was carried out using the medical database at the comprehensive care clinic for HIV patients in Consolata Hospital Nkubu, Meru county, Kenya

Beyond a unitary household measure: Does Gender matter in Legume Seed Systems among Smallholder Farmers?

We employ a non-unitary household model to analyze the main Pigeonpea seed channels for in Kenya. The paper is based on a household survey conducted on a 500 randomly selected households within three counties of Eastern Kenya. The study sites are based on the distance from the main trading center (county headquarters) which informs agro business infrastructure. We assess the participation in seed channels with regards to joint plots, women plots and male plots for Pigeonpeas legumes. More than half of the Pigeonpea plots (>50 %) in the sample are managed jointly by men and women; while around 10% are managed entirely by women. There were very few plots (<1%) managed by men alone. The main legumes seed sources are own saved seeds and cereal stockists. There is very limited sourcing from the certified seed channel (<10%), the certified seeds from agrovets are only acquired for joint plots. Using a multinomial logistic regression, we analyze the factors influencing the choice of Pigeonpea seed channels, encompassing characteristics of the wife of the household head. Literate wives and wives with high exposure to extension services were more likely to access seed from the agrovets, these variables were however not significant for the male head. Other significant determinants were total livestock unit, distance to the source of seed, amount of seed required, location of the household, and occupation. We conclude that targeting women farmers with knowledge and capacity building on the advantages of using certified seeds for legumes has the potential to enhance adoption of legumes in Eastern Kenya, education levels notwithstanding

Environmental Particle Emissions due to Automated Drilling of Polypropylene Composites and Nanocomposites Reinforced with Talc, Montmorillonite and Wollastonite

In this study, the effect on nanoparticle emissions due to drilling on Polypropylene (PP) reinforced with 20% talc, 5% montmorillonite (MMT) and 5% Wollastonite (WO) is investigated. The study is the first to explore the nanoparticle release from WO and talc reinforced composites and compares the results to previously researched MMT. With 5% WO, equivalent tensile properties with a 10 % weight reduction were obtained relative to the reference 20% talc sample. The materials were fabricated through injection moulding. The nanorelease studies were undertaken using the controlled drilling methodology for nanoparticle exposure assessment developed within the European Commission funded SIRENA Life 11 ENV/ES/506 project. Measurements were taken using CPC and DMS50 equipment for real-time characterization and measurements. The particle number concentration (of particles <1000nm) and particle size distribution (4.87nm - 562.34nm) of the particles emitted during drilling were evaluated to investigate the effect of the silicate fillers on the particles released. The nano-filled samples exhibited a 33% decrease (MMT sample) or a 30% increase (WO sample) on the average particle number concentration released in comparison to the neat polypropylene sample. The size distribution data displayed a substantial percentage of the particles released from the PP, PP/WO and PP/MMT samples to be between 5-20nm, whereas the PP/talc sample emitted larger particle diameters.The work is funded by and part of the European Commission Life project named Simulation of the release of nanomaterials from consumer products for environmental exposure assessment (SIRENA, Pr. No. LIFE 11 ENV/ES/596). The access and use of the facilities at the Flemish Institute for Technological Research (VITO) was funded by QualityNano Project through Transnational Access (TA Application VITO-TAF-382 and VITO-TAF-500) under the European Commission, Grant Agreement No: INFRA-2010-262163. Kristof is also thankful for partial funding by the School of Engineering at Robert Gordon University for his studentship

Pyrethrins In Soil and Water From Selected Pyrethrum Growing Areas In Nakuru County, Kenya

Introduction: Pyrethrum also known as Chrysanthemum is a plant from which compounds known as pyrethrins are derived. The pyrethrins have&nbsp; been used for many years as insecticides. Incidentally due to their high instability they have slowly been replaced by synthetic pyrethroids.&nbsp; Pyrethrins are generally regarded as safe compared to the pyrethroids. However, the amounts released into the environment have not been well documented especially in pyrethrum growing regions.Objective:&nbsp; The aim of the study was to determine the concentration of pyrethrins that come from pyrethrum plants and released into the&nbsp; environment through their use as insecticides, thus, into drinking water and soil, in pyrethrum growing regions in Kenya.Methodology: Quantification to amounts of pyrethrins from pyrethrum plants, in soil and water bodies in and around pyrethrum farms in Kiambogo and Naivasha (Nakuru County). The study was carried out using High Performance Liquid Chromatography (HPLC). Water samples (0.5L) were collected from the following water bodies: rivers, streams, dams, wells and boreholes near or within pyrethrum farms.Conclusion: It was established that, the quantity of pyrethrins present in water and soil samples werebelow detectable levels within the WHO recommended range. Hence safe for the environment,more so for the farmers and the people living around pyrethrum farms. Key words: Pyrethrum, pyrethrins, chromatography, water, soil

Determinants of antibody persistence across doses and continents after single-dose rVSV-ZEBOV vaccination for Ebola virus disease: an observational cohort study.

BACKGROUND: The recombinant vesicular stomatitis virus (rVSV) vaccine expressing the Zaire Ebola virus (ZEBOV) glycoprotein is efficacious in the weeks following single-dose injection, but duration of immunity is unknown. We aimed to assess antibody persistence at 1 and 2 years in volunteers who received single-dose rVSV-ZEBOV in three previous trials. METHODS: In this observational cohort study, we prospectively followed-up participants from the African and European phase 1 rVSV-ZEBOV trials, who were vaccinated once in 2014-15 with 300 000 (low dose) or 10-50 million (high dose) plaque-forming units (pfu) of rVSV-ZEBOV vaccine to assess ZEBOV glycoprotein (IgG) antibody persistence. The primary outcome was ZEBOV glycoprotein-specific IgG geometric mean concentrations (GMCs) measured yearly by ELISA compared with 1 month (ie, 28 days) after immunisation. We report GMCs up to 2 years (Geneva, Switzerland, including neutralising antibodies up to 6 months) and 1 year (Lambaréné, Gabon; Kilifi, Kenya) after vaccination and factors associated with higher antibody persistence beyond 6 months, according to multivariable analyses. Trials and the observational study were registered at ClinicalTrials.gov (Geneva: NCT02287480 and NCT02933931; Kilifi: NCT02296983) and the Pan-African Clinical Trials Registry (Lambaréné PACTR201411000919191). FINDINGS: Of 217 vaccinees from the original studies (102 from the Geneva study, 75 from the Lambaréné study, and 40 from the Kilifi study), 197 returned and provided samples at 1 year (95 from the Geneva study, 63 from the Lambaréné, and 39 from the Kilifi study) and 90 at 2 years (all from the Geneva study). In the Geneva group, 44 (100%) of 44 participants who had been given a high dose (ie, 10-50 million pfu) of vaccine and who were seropositive at day 28 remained seropositive at 2 years, whereas 33 (89%) of 37 who had been given the low dose (ie, 300 000 pfu) remained seropositive for 2 years (p=0·042). In participants who had received a high dose, ZEBOV glycoprotein IgG GMCs decreased significantly between their peak (at 1-3 months) and month 6 after vaccination in Geneva (p0·05). Neutralising antibodies seem to be less durable, with seropositivity dropping from 64-71% at 28 days to 27-31% at 6 months in participants from the Geneva study. INTERPRETATION: Antibody responses to single-dose rVSV-ZEBOV vaccination are sustained across dose ranges and settings, a key criterion in countries where booster vaccinations would be impractical. FUNDING: The Wellcome Trust and Innovative Medicines Initiative 2 Joint Undertaking

Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria.

Ring-infected erythrocytes are the predominant asexual stage in the peripheral circulation but are rarely investigated in the context of acquired immunity against Plasmodium falciparum malaria. Here we compare antibody-dependent phagocytosis of ring-infected parasite cultures in samples from a controlled human malaria infection (CHMI) study (NCT02739763). Protected volunteers did not develop clinical symptoms, maintained parasitaemia below a predefined threshold of 500 parasites/μl and were not treated until the end of the study. Antibody-dependent phagocytosis of both ring-infected and uninfected erythrocytes from parasite cultures was strongly correlated with protection. A surface proteomic analysis revealed the presence of merozoite proteins including erythrocyte binding antigen-175 and -140 on ring-infected and uninfected erythrocytes, providing an additional antibody-mediated protective mechanism for their activity beyond invasion-inhibition. Competition phagocytosis assays support the hypothesis that merozoite antigens are the key mediators of this functional activity. Targeting ring-stage parasites may contribute to the control of parasitaemia and prevention of clinical malaria

What drives capacity to innovate? Insights from women and men small-scale farmers in Africa, Asia, and Latin America

What are key characteristics of rural innovators? How are their experiences similar for women and men, and how are they different? To examine these questions, we draw on individual interviews with 336 rural women and men known in their communities for trying out new things in agriculture. The data form part of 84 GENNOVATE community case studies from 19 countries. Building on study participants’ own reflections and experiences with innovation in their agricultural livelihoods, we combine variable-oriented analysis and analysis of specific individuals’ lived experience. Results indicate that factors related to personality and agency are what most drive women’s and men’s capacity to innovate. Access to resources is not a prerequisite but rather an important enabling aspect. Different types of women have great potential for local innovation, but structural inequalities make men better positioned to access resources and leverage support. Men’s support is important when women challenge the status quo

Advances in Crop Improvement and Delivery Research for Nutritional Quality and Health Benefits of Groundnut (Arachis hypogaea L.)

Groundnut is an important global food and oil crop that underpins agriculture-dependent livelihood strategies meeting food, nutrition, and income security. Aflatoxins, pose a major challenge to increased competitiveness of groundnut limiting access to lucrative markets and affecting populations that consume it. Other drivers of low competitiveness include allergens and limited shelf life occasioned by low oleic acid profile in the oil. Thus grain off-takers such as consumers, domestic, and export markets as well as processors need solutions to increase profitability of the grain. There are some technological solutions to these challenges and this review paper highlights advances in crop improvement to enhance groundnut grain quality and nutrient profile for food, nutrition, and economic benefits. Significant advances have been made in setting the stage for marker-assisted allele pyramiding for different aflatoxin resistance mechanisms—in vitro seed colonization, pre-harvest aflatoxin contamination, and aflatoxin production—which, together with pre- and post-harvest management practices, will go a long way in mitigating the aflatoxin menace. A breakthrough in aflatoxin control is in sight with overexpression of antifungal plant defensins, and through host-induced gene silencing in the aflatoxin biosynthetic pathway. Similarly, genomic and biochemical approaches to allergen control are in good progress, with the identification of homologs of the allergen encoding genes and development of monoclonal antibody based ELISA protocol to screen for and quantify major allergens. Double mutation of the allotetraploid homeologous genes, FAD2A and FAD2B, has shown potential for achieving >75% oleic acid as demonstrated among introgression lines. Significant advances have been made in seed systems research to bridge the gap between trait discovery, deployment, and delivery through innovative partnerships and action learning