10,303 research outputs found
CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly
Human centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alphoid DNA sequences. By inducing rapid, complete degradation of endogenous CENP-A, we now demonstrate that once the first steps of centromere assembly have been completed in G1/S, continued CENP-A binding is not required for maintaining kinetochore attachment to centromeres or for centromere function in the next mitosis. Degradation of CENP-A prior to kinetochore assembly is found to block deposition of CENP-C and CENP-N, but not CENP-T, thereby producing defective kinetochores and failure of chromosome segregation. Without the continuing presence of CENP-A, CENP-B binding to alphoid DNA sequences becomes essential to preserve anchoring of CENP-C and the kinetochore to each centromere. Thus, there is a reciprocal interdependency of CENP-A chromatin and the underlying repetitive centromere DNA sequences bound by CENP-B in the maintenance of human chromosome segregation
A general scheme for modeling gamma-ray burst prompt emission
We describe a general method for modeling gamma-ray burst prompt emission. We
find that for the burst to be produced via the synchrotron process unphysical
conditions are required -- the distance of the source from the center of the
explosion () must be larger than cm and the source
Lorentz factor \gta 10^3; for such a high Lorentz factor the deceleration
radius () is less than even if the number density of particles
in the surrounding medium is as small as cm. The result,
, is in contradiction with the early x-ray and optical
afterglow data. The synchrotron-self-Compton (SSC) process fares much better.
There is a large solution space for a typical GRB prompt emission to be
produced via the SSC process. The prompt optical emission accompanying the
burst is found to be very bright (\lta 14 mag; for ) in the SSC
model, which exceeds the observed flux (or upper limit) for most GRBs.
Continuous acceleration of electrons can significantly reduce the optical flux
and bring it down to the observed limits. (Abridged)Comment: Published in MNRAS Jan 2008, 56 page
Simple Metals at High Pressure
In this lecture we review high-pressure phase transition sequences exhibited
by simple elements, looking at the examples of the main group I, II, IV, V, and
VI elements. General trends are established by analyzing the changes in
coordination number on compression. Experimentally found phase transitions and
crystal structures are discussed with a brief description of the present
theoretical picture.Comment: 22 pages, 4 figures, lecture notes for the lecture given at the Erice
course on High-Pressure Crystallography in June 2009, Sicily, Ital
Molecular motion in cell membranes: analytic study of fence-hindered random walks
A theoretical calculation is presented to describe the confined motion of
transmembrane molecules in cell membranes. The study is analytic, based on
Master equations for the probability of the molecules moving as random walkers,
and leads to explicit usable solutions including expressions for the molecular
mean square displacement and effective diffusion constants. One outcome is a
detailed understanding of the dependence of the time variation of the mean
square displacement on the initial placement of the molecule within the
confined region. How to use the calculations is illustrated by extracting
(confinement) compartment sizes from experimentally reported published
observations from single particle tracking experiments on the diffusion of
gold-tagged G-protein coupled mu-opioid receptors in the normal rat kidney cell
membrane, and by further comparing the analytical results to observations on
the diffusion of phospholipids, also in normal rat kidney cells.Comment: 10 pages, 5 figure
The Cosmic Horseshoe: Discovery of an Einstein Ring around a Giant Luminous Red Galaxy
We report the discovery of an almost complete Einstein ring of diameter 10"
in Sloan Digital Sky Survey (SDSS) Data Release 5 (DR5). Spectroscopic data
from the 6m telescope of the Special Astrophysical Observatory reveals that the
deflecting galaxy has a line-of-sight velocity dispersion in excess of 400 km/s
and a redshift of 0.444, whilst the source is a star-forming galaxy with a
redshift of 2.379. From its color and luminosity, we conclude that the lens is
an exceptionally massive Luminous Red Galaxy (LRG) with a mass within the
Einstein radius of 5 x 10^12 solar masses. This remarkable system provides a
laboratory for probing the dark matter distribution in LRGs at distances out to
3 effective radii, and studying the properties of high redshift star-forming
galaxies.Comment: ApJ (Letters), in pres
Elevated virulence of an emerging viral genotype as a driver of honeybee loss.
PublishedJournal ArticleEmerging infectious diseases (EIDs) have contributed significantly to the current biodiversity crisis, leading to widespread epidemics and population loss. Owing to genetic variation in pathogen virulence, a complete understanding of species decline requires the accurate identification and characterization of EIDs. We explore this issue in the Western honeybee, where increasing mortality of populations in the Northern Hemisphere has caused major concern. Specifically, we investigate the importance of genetic identity of the main suspect in mortality, deformed wing virus (DWV), in driving honeybee loss. Using laboratory experiments and a systematic field survey, we demonstrate that an emerging DWV genotype (DWV-B) is more virulent than the established DWV genotype (DWV-A) and is widespread in the landscape. Furthermore, we show in a simple model that colonies infected with DWV-B collapse sooner than colonies infected with DWV-A. We also identify potential for rapid DWV evolution by revealing extensive genome-wide recombination in vivo The emergence of DWV-B in naive honeybee populations, including via recombination with DWV-A, could be of significant ecological and economic importance. Our findings emphasize that knowledge of pathogen genetic identity and diversity is critical to understanding drivers of species decline.This work was supported by the Federal Ministry of Food,
Agriculture and Consumer Protection (Germany): Fit Bee project
(grant 511-06.01-28-1-71.007-10), the EU: BeeDoc (grant 244956),
iDiv (2013 NGS-Fast Track grant W47004118) and the Insect Pollinators
Initiative (IPI grant BB/I000100/1 and BB/I000151/1). The IPI is
funded jointly by the Biotechnology and Biological Sciences Research
Council, the Department for Environment, Food and Rural Affairs,
the Natural Environment Research Council, the Scottish Government
and the Wellcome Trust, under the Living with Environmental
Change Partnership
Total energy calculation of high pressure selenium: The origin of incommensurate modulations in Se-IV and the instability of proposed Se-II
We present calculation of the high pressure crystal structures in selenium,
including rational approximants to the recently reported incommensurate phases.
We show how the incommensurate phases can be intuitively explained in terms of
imaginary phonon frequencies arising from Kohn anomalies in the putative
undistorted phase. We also find inconsistencies between the calculated and
experimental Se-II phase - the calculations show it to be a metastable metal
while the experiment finds a stable semiconductor. We propose that the
experimentally reported structure is probably in error.Comment: 4 pages 4 figure
Radio Properties of z>4 Optically-Selected Quasars
We report on two programs to address differential evolution between the
radio-loud and radio-quiet quasar populations at high (z>4) redshift. Both
programs entail studying the radio properties of optically-selected quasars.
First, we have observed 32 optically-selected, high-redshift (z>4) quasars with
the VLA at 6 cm (5 GHz). These sources comprise a statistically complete and
well-understood sample. We detect four quasars above our 3-sigma limit of ~0.15
mJy, which is sufficiently sensitive to detect all radio-loud quasars at the
probed redshift range. Second, we have correlated 134 z>4 quasars, comprising
all such sources that we are aware of as of mid-1999, with FIRST and NVSS.
These two recent 1.4 GHz VLA sky surveys reach 3-sigma limits of approximately
0.6 mJy and 1.4 mJy respectively. We identify a total of 15 z>4 quasars, of
which six were not previously known to be radio-loud. The depth of these
surveys does not reach the radio-loud/radio-quiet demarcation luminosity
density (L(1.4 GHz) = 10^32.5 h(50)^(-2) ergs/s/Hz) at the redshift range
considered; this correlation therefore only provides a lower limit to the
radio-loud fraction of quasars at high-redshift. The two programs together
identify eight new radio-loud quasars at z>4, a significant increase over the
seven currently in the published literature. We find no evidence for radio-loud
fraction depending on optical luminosity for -25 > M_B > -28 at z~2, or for
-26>M_B>-28 at z>4. Our results also show no evolution in the radio-loud
fraction between z~2 and z>4 (-26>M_B>-28).Comment: 19 pages, 7 figures; to appear in The Astronomical Journal (April
2000
Photochemistry of Furyl- and Thienyldiazomethanes: Spectroscopic Characterization of Triplet 3-Thienylcarbene
Photolysis (λ \u3e 543 nm) of 3-thienyldiazomethane (1), matrix isolated in Ar or N2 at 10 K, yields triplet 3-thienylcarbene (13) and α-thial-methylenecyclopropene (9). Carbene 13 was characterized by IR, UV/vis, and EPR spectroscopy. The conformational isomers of 3-thienylcarbene (s-E and s-Z) exhibit an unusually large difference in zero-field splitting parameters in the triplet EPR spectrum (|D/hc| = 0.508 cm–1, |E/hc| = 0.0554 cm–1; |D/hc| = 0.579 cm–1, |E/hc| = 0.0315 cm–1). Natural Bond Orbital (NBO) calculations reveal substantially differing spin densities in the 3-thienyl ring at the positions adjacent to the carbene center, which is one factor contributing to the large difference in D values. NBO calculations also reveal a stabilizing interaction between the sp orbital of the carbene carbon in the s-Z rotamer of 13 and the antibonding σ orbital between sulfur and the neighboring carbon—an interaction that is not observed in the s-E rotamer of 13. In contrast to the EPR spectra, the electronic absorption spectra of the rotamers of triplet 3-thienylcarbene (13) are indistinguishable under our experimental conditions. The carbene exhibits a weak electronic absorption in the visible spectrum (λmax = 467 nm) that is characteristic of triplet arylcarbenes. Although studies of 2-thienyldiazomethane (2), 3-furyldiazomethane (3), or 2-furyldiazomethane (4) provided further insight into the photochemical interconversions among C5H4S or C5H4O isomers, these studies did not lead to the spectroscopic detection of the corresponding triplet carbenes (2-thienylcarbene (11), 3-furylcarbene (23), or 2-furylcarbene (22), respectively)
A Combine On-Line Acoustic Flowmeter and Fluorocarbon Coolant Mixture Analyzer for The ATLAS Silicon Tracker
An upgrade to the ATLAS silicon tracker cooling control system may require a
change from C3F8 (octafluoro-propane) to a blend containing 10-30% of C2F6
(hexafluoro-ethane) to reduce the evaporation temperature and better protect
the silicon from cumulative radiation damage with increasing LHC luminosity.
Central to this upgrade is a new acoustic instrument for the real-time
measurement of the C3F8/C2F6 mixture ratio and flow. The instrument and its
Supervisory, Control and Data Acquisition (SCADA) software are described in
this paper. The instrument has demonstrated a resolution of 3.10-3 for
C3F8/C2F6 mixtures with ~20%C2F6, and flow resolution of 2% of full scale for
mass flows up to 30gs-1. In mixtures of widely-differing molecular weight (mw),
higher mixture precision is possible: a sensitivity of < 5.10-4 to leaks of
C3F8 into the ATLAS pixel detector nitrogen envelope (mw difference 160) has
been seen. The instrument has many potential applications, including the
analysis of mixtures of hydrocarbons, vapours for semi-conductor manufacture
and anaesthesia
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