Capacidad de realizar esprints repetidos en jugadores profesionales de fútbol vs. Fútbol sala

Objetivo: Investigar los cambios producidos ante acciones de esprines repetidos (20+20 metros con cambio de dirección ), el CMJ, la respuesta metabólica (lactato) , y la relación entre estas variables y la fuerza y resistencia en jugadores profesionales de fútbol y fútbol sala. Métodos: Jugadores profesionales (n=30, doce de fútbol sala y veinte de fútbol) completaron tres sesiones de evaluación; VO2max en tapiz rodante, el salto CMJ y la RM en sentadilla completa y finalmente, se realizó el test de RSA (6 x 40 (20+20)). Resultados: Se obtuvieron valores similares de fuerza en sentadilla, en CMJ, de LAC después de la prueba RSA y de VO2max ( 95,12 kg vs 94,73 kg ; 34,5 cm vs 35,9 cm ; 13,65 mmol vs 14,33 mmol ; 62,78 ml·kg·min-1 vs . 62,95 ml·kg·min-1 fútbol vs. fútbol sala, respectivamente). Se obtuvieron diferencias significativas cuando se analizó la pérdida de rendimiento en velocidad (total y entre los tres primeros y tres últimos bloques de esprines) y en salto (2,67% vs 4,4%**; 1,28% vs 2,1%*; 2,88% vs 6,1%**; 9,71% vs 14,3%* de fútbol vs. fútbol sala, respectivamente). Conclusiones: Ambos deportes presentan diferencias significativas en las pérdidas de rendimiento en velocidad y salto a pesar de tener valores similares de fuerza, de VO2max, de lactato tras RSA y de CMJ. Podría atribuirse a los esfuerzos propios de cada deporte y puede sugerir que debería incrementarse el volumen de entrenamiento orientado a la capacidad de realizar esfuerzos repetidos frente a otro tipo de objetivo como la mejora de la capacidad aeróbica, sobre todo a nivel profesional.To investigate the changes through repeated explosive effort sequences (20+20-m sprint with change of direction), jumping, metabolic response (lactate), as well as the relationship between these variables and fitness qualities (strength and endurance) in professional futsal and soccer players. Methods: Male players (n =30, Twelve futsal and twenty soccer players) completed three testing sessions. In the first session was measured VO2max on a motorized treadmill. In the second session was measured counter movement jump (CMJ) and full squat RM in Smith Machine. Finally, in the third session six repeated-explosive effort sequences (RES) was performed. Results: Similar values of lower limbs strength, CMJ height, LAC after RSA test and VO2max (95,12 vs. 94,73; 34,5 vs. 35,9; 13,65 vs. 14,33; 62,78 vs. 62,95 soccer vs. futsal respectively) and significant differences when are analysed the loss of performance in velocity (total and between three first and three last) and vertical jump height (2,67 vs. 4,4**; 1,28 vs. 2,1*; 2,88 vs. 6,1**; 9,71 vs. 14,3* soccer vs. futsal respectively). Conclusions: Professional futsal and soccer obtain significant differences in speed and vertical jump height (CMJ) loss despite having similar values in squat, oxygen consumption, lactate after RSA test and CMJ height. Issue that could be attributed to the characteristics of the sport. This suggests that the volume should be increased oriented ability to perform repeated sprint actions over other type of training aimed at improving aerobic capacity especially at professional level.peerReviewe

Tetrahydropyrazolo[1,5-a]Pyrimidine-3-Carboxamide and N-Benzyl-6′,7′-Dihydrospiro[Piperidine-4,4′-Thieno[3,2-c]Pyran] analogues with bactericidal efficacy against Mycobacterium tuberculosis targeting MmpL3

Mycobacterium tuberculosis is a major human pathogen and the causative agent for the pulmonary disease, tuberculosis (TB). Current treatment programs to combat TB are under threat due to the emergence of multi-drug and extensively-drug resistant TB. As part of our efforts towards the discovery of new anti-tubercular leads, a number of potent tetrahydropyrazolo[1,5-a]pyrimidine-3-ca​rboxamide(THPP) and N-benzyl-6′,7′-dihydrospiro[piperidine-4,​4′-thieno[3,2-c]pyran](Spiro) analogues were recently identified against Mycobacterium tuberculosis and Mycobacterium bovis BCG through a high-throughput whole-cell screening campaign. Herein, we describe the attractive in vitro and in vivo anti-tubercular profiles of both lead series. The generation of M. tuberculosis spontaneous mutants and subsequent whole genome sequencing of several resistant mutants identified single mutations in the essential mmpL3 gene. This ‘genetic phenotype’ was further confirmed by a ‘chemical phenotype’, whereby M. bovis BCG treated with both the THPP and Spiro series resulted in the accumulation of trehalose monomycolate. In vivo efficacy evaluation of two optimized THPP and Spiro leads showed how the compounds were able to reduce >2 logs bacterial cfu counts in the lungs of infected mice

Control de calidad y de almacenamiento de semillas de girasol mediante tecnología de infrarrojo cercano

This work assesses the application of near infrared spectroscopy technology for the quality control of sunflower seeds direct from farmers and from a storage silo. The results show that the analytical method employing near infrared spectroscopy can be used as a rapid and non-destructive tool for the determination of moisture, fat and high/low oleic acid contents in samples of sunflower seeds. The ranges obtained were comparable to those reported for classic chemical methods, and were between 4.6-21.4% for moisture; 38.4-49.6% for fat, and 60.0-93.1% for oleic acid expressed as percentage of total fatty acids. A stepwise discriminant analysis was performed to determine the most useful wavelengths for classifying sunflower seeds in terms of their (high/low) oleic acid composition. The discriminant model allows the classification of sunflower seeds with high or low oleic acid contents, with a prediction rate of 90.5% for internal validation and of 89.4% for cross-validation.En este trabajo se evalúa la espectroscopía de infrarrojo cercano para su uso en el control de calidad y almacenamiento de semillas de girasol. Los resultados indican que el método analítico empleado puede utilizarse como método de determinación rápida de humedad, grasa y contenidos altos/bajos de ácido oleico. Los rangos de aplicación son comparables con los valores que se han determinado mediante métodos clásicos de análisis, encontrándose entre 4.6-21.4% la humedad, 38.4-49.6% la grasa y 60.0- 93.1% de ácido oleico del total de los ácidos grasos. Además se ha utilizado un análisis discriminarte lineal por pasos determinando las longitudes de onda más adecuadas para la clasificación de semillas de girasol en los grupos alto/bajo oleico. El modelo generado permitió la clasificación de semillas de girasol en los grupos alto y bajo oleico con unos porcentajes de muestras correctamente clasificadas de un 90.5% en validación interna y de un 89.4% en validación cruzada

Plaque-Associated Oligomeric Amyloid-Beta Drives Early Synaptotoxicity in APP/PS1 Mice Hippocampus: Ultrastructural Pathology Analysis

Alzheimer’s disease (AD) is a devastating neurodegenerative disorder characterized by initial memory impairments that progress to dementia. In this sense, synaptic dysfunction and loss have been established as the pathological features that best correlate with the typical early cognitive decline in this disease. At the histopathological level, post mortem AD brains typically exhibit intraneuronal neurofibrillary tangles (NFTs) along with the accumulation of amyloid-beta (Abeta) peptides in the form of extracellular deposits. Specifically, the oligomeric soluble forms of Abeta are considered the most synaptotoxic species. In addition, neuritic plaques are Abeta deposits surrounded by activated microglia and astroglia cells together with abnormal swellings of neuronal processes named dystrophic neurites. These periplaque aberrant neurites are mostly presynaptic elements and represent the first pathological indicator of synaptic dysfunction. In terms of losing synaptic proteins, the hippocampus is one of the brain regions most affected in AD patients. In this work, we report an early decline in spatial memory, along with hippocampal synaptic changes, in an amyloidogenic APP/PS1 transgenic model. Quantitative electron microscopy revealed a spatial synaptotoxic pattern around neuritic plaques with significant loss of periplaque synaptic terminals, showing rising synapse loss close to the border, especially in larger plaques. Moreover, dystrophic presynapses were filled with autophagic vesicles in detriment of the presynaptic vesicular density, probably interfering with synaptic function at very early synaptopathological disease stages. Electron immunogold labeling showed that the periphery of amyloid plaques, and the associated dystrophic neurites, was enriched in Abeta oligomers supporting an extracellular location of the synaptotoxins. Finally, the incubation of primary neurons with soluble fractions derived from 6-month-old APP/PS1 hippocampus induced significant loss of synaptic proteins, but not neuronal death. Indeed, this preclinical transgenic model could serve to investigate therapies targeted at initial stages of synaptic dysfunction relevant to the prodromal and early AD.This study was supported by the Instituto de Salud Carlos III (ISCiii) of Spain, co-financed by the FEDER funds from European Union, through grants PI18/01557 (to AG) and PI18/01556 (to JV); by the Junta de Andalucia Consejería de Economía y Conocimiento through grants UMA18-FEDERJA-211 (to AG), P18-RT-2233 (to AG), and US-1262734 (to JV) co-financed by Programa Operativo FEDER 2014–2020; by the Spanish Minister of Science and Innovation grant PID2019-108911RA-100 (to DB-V), Beatriz Galindo program BAGAL18/00052 (to DB-V) grant PID2019-107090RA-I00 (to IM-G), and Ramon y Cajal Program RYC-2017-21879 (to IM-G); and by the Malaga University grants B1-2019_07 (to ES-M) and B1-2019_06 (to IM-G). MM-O held a predoctoral contract from Malaga University and ES-M a postdoctoral contract (DOC_00251) from Junta de Andalucia

When One Hemisphere Takes Control: Metacontrol in Pigeons (Columba livia)

Vertebrate brains are composed of two hemispheres that receive input, compute, and interact to form a unified response. How the partially different processes of both hemispheres are integrated to create a single output is largely unknown. In some cases one hemisphere takes charge of the response selection--a process known as metacontrol. Thus far, this phenomenon has only been shown in a handful of studies with primates, mostly conducted in humans. Metacontrol, however, is even more relevant for animals like birds with laterally placed eyes and complete chiasmatic decussation since visual input to the hemispheres is largely different.Homing pigeons (Columba livia) were trained with a color discrimination task. Each hemisphere was trained with a different color pair and therefore had a different experience. Subsequently, the pigeons were binocularly examined with two additional stimuli that combined the positive color of one hemisphere with a negative color that had been shown to the other, omitting the availability of a coherent solution and confronting the pigeons with a conflicting situation. Some of the pigeons responded to both stimuli, indicating that none of the hemispheres dominated the overall preference. Some birds, however, responded primarily to one of the conflicting stimuli, showing that they based their choice on the left- or right-monocularly learned color pair, indicating hemispheric metacontrol.We could demonstrate for the first time that metacontrol is a widespread phenomenon that also exists in birds, and thus in principle requires no corpus callosum. Our results are closely similar to those in humans: monocular performance was higher than binocular one and animals displayed different modes of hemispheric dominance. Thus, metacontrol is a dynamic and widely distributed process that possibly constitutes a requirement for all animals with a bipartite brain to confront the problem of choosing between two hemisphere-bound behavioral options

Young Exoplanet Transit Initiative follow-up observations of the T Tauri star CVSO 30 with transit-like dips

The T Tauri star CVSO 30, also known as PTFO 8-8695, was studied intensively with ground-based telescopes as well as with satellites over the last decade. It showed a variable light curve with additional repeating planetary transit-like dips every ∼10.8 h. However, these dimming events changed in depth and duration since their discovery and from autumn 2018 on, they were not even present or near the predicted observing times. As reason for the detected dips and their changes within the complex light curve, e.g. a disintegrating planet, a circumstellar dust clump, stellar spots, possible multiplicity, and orbiting clouds at a Keplerian co-rotating radius were discussed and are still under debate. In this paper, we present additional optical monitoring of CVSO 30 with the meter class telescopes of the Young Exoplanet Transit Initiative in Asia and Europe over the last 7 yr and characterize CVSO 30 with the new Early Data Release 3 of the European Space Agency-Gaia mission. As a result, we describe the evolution of the dimming events in the optical wavelength range since 2014 and present explanatory approaches for the observed variabilities. We conclude that orbiting clouds of gas at a Keplerian co-rotating radius are the most promising scenario to explain most changes in CVSO 30’s light curve. © 2022 The Author(s).This work is based on observations obtained with telescopes of the University Observatory Jena, operated by the Astrophysical Institute of the Friedrich-Schiller-Universität Jena. We thank B. Baghdasaryan, N. Belko, S. Buder, M. Dadalauri, M. Geymeier, H. Gilbert, A. Gonzalez, F. Hildebrandt, H. Keppler, O. Lux, S. Masda, P. Protte, J. Trautmann, A. Trepanowski, and S. Schlagenhauf, who have been involved in some observations of this project, obtained at the University Observatory Jena. This research was partly based on data obtained at the 1.5 m telescope of the Sierra Nevada Observatory (Spain), which is operated by the Consejo Superior de Investigaciones Científicas (CSIC) through the Instituto de Astrofísica de Andalucía. We thank J.F. Aceituno and V. Casanova for their help with the observations. This publication is partly based on observations made with ESO Telescopes at the La Silla Paranal Observatory under programme ID 090.C-0448(A). RB, RN, and MM acknowledge the support of the DFG priority programme SPP 1992 ‘Exploring the Diversity of Extrasolar Planets’ in projects NE 515/58-1 and MU 2695/27-1. We acknowledge financial support from the Spanish Agencia Estatal de Investigación of the Ministerio de Ciencia, Innovación y Universidades and the ERDF through projects PID2019-109522GB-C52 and AYA2016-79425-C3-3-P, and the Centre of Excellence ‘Severo Ochoa’ award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709). We thank R. Errmann for developing and providing the photometry routine ‘AUTOMAT.PY’ and also C. Broeg for his program ‘PHOTOMETRY’. This publication makes use of data products of the SIMBAD and VizieR data bases, operated at CDS, Strasbourg, France. We also thank the Gaia Data Processing and Analysis Consortium of the ESA for processing and providing the data of the Gaia mission. We thank the MAST portal for providing the TESS photometric results and the MESA Isochrones & Stellar Tracks website for the bolometric correction tables.Peer reviewe