Mass modelling globular clusters in the Gaia era: a method comparison using mock data from an N-body simulation of M 4

As we enter a golden age for studies of internal kinematics and dynamics of Galactic globular clusters (GCs), it is timely to assess the performance of modelling techniques in recovering the mass, mass profile, and other dynamical properties of GCs. Here, we compare different mass-modelling techniques (distribution function (DF)-based models, Jeans models, and a grid of N-body models) by applying them to mock observations from a star-by-star N-body simulation of the GC M 4 by Heggie. The mocks mimic existing and anticipated data for GCs: surface brightness or number density profiles, local stellar mass functions, line-of-sight velocities, and Hubble Space Telescope-and Gaia-like proper motions. We discuss the successes and limitations of the methods. We find that multimass DF-based models, Jeans, and N-body models provide more accurate mass profiles compared to single-mass DF-based models. We highlight complications in fitting the kinematics in the outskirts due to energetically unbound stars associated with the cluster ('potential escapers', captured neither by truncated DF models nor by N-body models of clusters in isolation), which can be avoided with DF-based models including potential escapers, or with Jeans models. We discuss ways to account for mass segregation. For example, three-component DF-based models with freedom in their mass function are a simple alternative to avoid the biases of single-mass models (which systematically underestimate the total mass, half-mass radius, and central density), while more realistic multimass DF-based models with freedom in the remnant content represent a promising avenue to infer the total mass and the mass function of remnants

Globular cluster number density profiles using Gaia DR2

Using data from Gaia DR2, we study the radial number density profiles of the Galactic globular cluster sample. Proper motions are used for accurate membership selection, especially crucial in the cluster outskirts. Due to the severe crowding in the centres, the Gaia data are supplemented by literature data from HST and surface brightness measurements, where available. This results in 81 clusters with a complete density profile covering the full tidal radius (and beyond) for each cluster. We model the density profiles using a set of single-mass models ranging from King and Wilson models to generalized lowered isothermal LIMEPY models and the recently introduced SPES models, which allow for the inclusion of potential escapers. We find that both King and Wilson models are too simple to fully reproduce the density profiles, with King (Wilson) models on average underestimating (overestimating) the radial extent of the clusters. The truncation radii derived from the LIMEPY models are similar to estimates for the Jacobi radii based on the cluster masses and their orbits. We show clear correlations between structural and environmental parameters, as a function of Galactocentric radius and integrated luminosity. Notably, the recovered fraction of potential escapers correlates with cluster pericentre radius, luminosity, and cluster concentration. The ratio of half mass over Jacobi radius also correlates with both truncation parameter and PE fraction, showing the effect of Roche lobe filling

Globular cluster number density profiles using Gaia DR2

Using data from Gaia DR2, we study the radial number density profiles of the Galactic globular cluster sample. Proper motions are used for accurate membership selection, especially crucial in the cluster outskirts. Due to the severe crowding in the centres, the Gaia data is supplemented by literature data from HST and surface brightness measurements, where available. This results in 81 clusters with a complete density profile covering the full tidal radius (and beyond) for each cluster. We model the density profiles using a set of single-mass models ranging from King and Wilson models to generalised lowered isothermal limepy models and the recently introduced spes models, which allow for the inclusion of potential escapers. We find that both King and Wilson models are too simple to fully reproduce the density profiles, with King (Wilson) models on average underestimating(overestimating) the radial extent of the clusters. The truncation radii derived from the limepy models are similar to estimates for the Jacobi radii based on the cluster masses and their orbits. We show clear correlations between structural and environmental parameters, as a function of Galactocentric radius and integrated luminosity. Notably, the recovered fraction of potential escapers correlates with cluster pericentre radius, luminosity and cluster concentration. The ratio of half mass over Jacobi radius also correlates with both truncation parameter and PE fraction, showing the effect of Roche lobe filling.Comment: 23 pages, 23 figures. Accepted in MNRA

Fungal volatile organic compounds: emphasis on their plant growth-promoting

Fungal volatile organic compounds (VOCs) commonly formed bioactive interface between plants and countless of microorganisms on the above- and below-ground plant-fungus interactions. Fungal-plant interactions symbolize intriguingly biochemical complex and challenging scenarios that are discovered by metabolomic approaches. Remarkably secondary metabolites (SMs) played a significant role in the virulence and existence with plant-fungal pathogen interaction; only 25% of the fungal gene clusters have been functionally identified, even though these numbers are too low as compared with plant secondary metabolites. The current insights on fungal VOCs are conducted under lab environments and to apply small numbers of microbes; its molecules have significant effects on growth, development, and defense system of plants. Many fungal VOCs supported dynamic processes, leading to countless interactions between plants, antagonists, and mutualistic symbionts. The fundamental role of fungal VOCs at field level is required for better understanding, so more studies will offer further constructive scientific evidences that can show the cost-effectiveness of ecofriendly and ecologically produced fungal VOCs for crop welfare

Ustekinumab as Induction and Maintenance Therapy for Crohn’s Disease

BACKGROUND Ustekinumab, a monoclonal antibody to the p40 subunit of interleukin-12 and inter-leukin-23, was evaluated as an intravenous induction therapy in two populations with moderately to severely active Crohn’s disease. Ustekinumab was also evaluated as subcutaneous maintenance therapy. METHODS We randomly assigned patients to receive a single intravenous dose of ustekinumab (either 130 mg or approximately 6 mg per kilogram of body weight) or placebo in two induction trials. The UNITI-1 trial included 741 patients who met the criteria for primary or secondary nonresponse to tumor necrosis factor (TNF) antagonists or had unacceptable side effects. The UNITI-2 trial included 628 patients in whom conventional therapy failed or unacceptable side effects occurred. Patients who completed these induction trials then participated in IM-UNITI, in which the 397 patients who had a response to ustekinumab were randomly assigned to receive subcutaneous maintenance injections of 90 mg of ustekinumab (either every 8 weeks or every 12 weeks) or placebo. The primary end point for the induction trials was a clinical response at week 6 (defined as a decrease from baseline in the Crohn’s Disease Activity Index [CDAI] score of ≥100 points or a CDAI score <150). The primary end point for the maintenance trial was remission at week 44 (CDAI score <150). RESULTS The rates of response at week 6 among patients receiving intravenous ustekinumab at a dose of either 130 mg or approximately 6 mg per kilogram were significantly higher than the rates among patients receiving placebo (in UNITI-1, 34.3%, 33.7%, and 21.5%, respectively, with P≤0.003 for both comparisons with placebo; in UNITI-2, 51.7%, 55.5%, and 28.7%, respectively, with P<0.001 for both doses). In the groups receiving maintenance doses of ustekinumab every 8 weeks or every 12 weeks, 53.1% and 48.8%, respectively, were in remission at week 44, as compared with 35.9% of those receiving placebo (P = 0.005 and P = 0.04, respectively). Within each trial, adverse-event rates were similar among treatment groups. CONCLUSIONS Among patients with moderately to severely active Crohn’s disease, those receiving intravenous ustekinumab had a significantly higher rate of response than did those receiving placebo. Subcutaneous ustekinumab maintained remission in patients who had a clinical response to induction therapy. (Funded by Janssen Research and Development; ClinicalTrials.gov numbers, NCT01369329, NCT01369342, and NCT01369355.

Transcutaneous electrical nerve stimulation reduces exercise-induced perceived pain and improves endurance exercise performance

Purpose. Muscle pain is a natural consequence of intense and prolonged exercise and has been suggested to be a limiter of performance. Transcutaneous electrical nerve stimulation (TENS) and interferential current (IFC) have been shown to reduce both chronic and acute pain in a variety of conditions. This study sought to ascertain whether TENS and IFC could reduce exercise-induced pain (EIP) and whether this would affect exercise performance. It was hypothesised that TENS and IFC would reduce EIP and result in an improved exercise performance. Methods. In two parts, 18 (Part I) and 22 (Part II) healthy male and female participants completed an isometric contraction of the dominant bicep until exhaustion (Part I) and a 16.1 km cycling time trial as quickly as they could (Part II) whilst receiving TENS, IFC and a SHAM placebo in a repeated measures, randomized cross-over, and placebo controlled design. Perceived EIP was recorded in both tasks using a validated subjective scale. Results. In Part I, TENS significantly reduced perceived EIP (mean reduction of 12%) during the isometric contraction (P = 0.006) and significantly improved participants’ time to exhaustion by a mean of 38% (P = 0.02). In Part II, TENS significantly improved (P = 0.003) participants’ time trial completion time (~2% improvement) through an increased mean power output. Conclusion. These findings demonstrate that TENS can attenuate perceived EIP in a healthy population and that doing so significantly improves endurance performance in both submaximal isometric single limb exercise and whole-body dynamic exercise