1,497 research outputs found
Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors.
Abstract
This study examines the regulation of cGMP by illumination and by calcium during signal transduction in vertebrate retinal photoreceptor cells. We employed an electropermeabilized rod outer segment (EP-ROS) preparation which permits perfusion of low molecular weight compounds into the cytosol while retaining many of the features of physiologically competent, intact rod outer segments (ROS). When nucleotide-depleted EP-ROS were incubated with MgGTP, time- and dose-dependent increases in intracellular cGMP levels were observed. The steady state cGMP concentration in EP-ROS (0.007 mol cGMP per mol rhodopsin) approached the cGMP concentration in intact ROS. Flash illumination of EP-ROS in a 250-nM free calcium medium resulted in a transient decrease in cGMP levels; this occurred in the absence of changes in calcium concentration. The kinetics of the cGMP response to flash illumination of EP-ROS were similar to that of intact ROS. To further examine the effects of calcium on cGMP metabolism, dark-adapted EP-ROS were incubated with MgGTP containing various concentrations of calcium. We observed a twofold increase in cGMP steady state levels as the free calcium was lowered from 1 μM to 20 nM; this increase was comparable to the behavior of intact ROS. Measurements of guanylate cyclase activity in EP-ROS showed a 3.5-fold increase in activity over this range of calcium concentrations, indicating a retention of calcium regulation of guanylate cyclase in EP-ROS preparations. Flash illumination of EP-ROS in either a 50- or 250-nM free calcium medium revealed a slowing of the recovery time course at the lower calcium concentration. This observation conflicts with any hypothesis whereby a reduction in free calcium concentration hastens the recovery of cytoplasmic cGMP levels, either by stimulating guanylate cyclase activity or by inhibiting phosphodiesterase activity. We conclude that changes in the intracellular calcium concentration during visual transduction may have more complex effects on the recovery of the photoresponse than can be accounted for solely by guanylate cyclase activation
Gravitational Wave Radiation from Compact Binary Systems in the Jordan-Brans-Dicke Theory
In this paper we analyze the signal emitted by a compact binary system in the Jordan-Brans-Dicke theory. We compute the scalar and tensor components of the power radiated by the source and study the scalar waveform. Eventually we consider the detectability of the scalar component of the radiation by interferometers and resonant-mass detectors
Testing Theories of Gravity with a Spherical Gravitational Wave Detector
We consider the possibility of discriminating different theories of gravity
using a recently proposed gravitational wave detector of spherical shape. We
argue that the spin content of different theories can be extracted relating the
measurements of the excited spheroidal vibrational eigenmodes to the
Newman-Penrose parameters. The sphere toroidal modes cannot be excited by any
metric GW and can be thus used as a veto.Comment: latex file, 16 pages, 1 figur
Dark Matter searches using gravitational wave bar detectors: quark nuggets and newtorites
Many experiments have searched for supersymmetric WIMP dark matter, with null
results. This may suggest to look for more exotic possibilities, for example
compact ultra-dense quark nuggets, widely discussed in literature with several
different names. Nuclearites are an example of candidate compact objects with
atomic size cross section. After a short discussion on nuclearites, the result
of a nuclearite search with the gravitational wave bar detectors Nautilus and
Explorer is reported. The geometrical acceptance of the bar detectors is 19.5
sr, that is smaller than that of other detectors used for similar
searches. However, the detection mechanism is completely different and is more
straightforward than in other detectors. The experimental limits we obtain are
of interest because, for nuclearites of mass less than g, we find a
flux smaller than that one predicted considering nuclearites as dark matter
candidates. Particles with gravitational only interactions (newtorites) are
another example. In this case the sensitivity is quite poor and a short
discussion is reported on possible improvements.Comment: published on Astroparticle Physics Sept 25th 2016 replaced fig 1
Quark nuggets search using 2350 Kg gravitational waves aluminum bar detectors
The gravitational wave resonant detectors can be used as detectors of quark
nuggets, like nuclearites (nuclear matter with a strange quark). This search
has been carried out using data from two 2350 Kg, 2 K cooled, aluminum bar
detectors: NAUTILUS, located in Frascati (Italy), and EXPLORER, that was
located in CERN Geneva (CH). Both antennas are equipped with cosmic ray shower
detectors: signals in the bar due to showers are continuously detected and used
to characterize the antenna performances. The bar excitation mechanism is based
on the so called thermo-acoustic effect, studied on dedicated experiments that
use particle beams. This mechanism predicts that vibrations of bars are induced
by the heat deposited in the bar from the particle. The geometrical acceptance
of the bar detectors is 19.5 sr, that is smaller than that of other
detectors used for similar searches. However, the detection mechanism is
completely different and is more straightforward than in other detectors. We
will show the results of ten years of data from NAUTILUS (2003-2012) and 7
years from EXPLORER (2003-2009). The experimental limits we obtain are of
interest because, for nuclearites of mass less than grams, we find a
flux smaller than that one predicted considering nuclearites as dark matter
candidates.Comment: presented to the 33rd International Cosmic Ray Conference Rio de
Janeiro 201
Analysis of 3 years of data from the gravitational wave detectors EXPLORER and NAUTILUS
We performed a search for short gravitational wave bursts using about 3 years
of data of the resonant bar detectors Nautilus and Explorer. Two types of
analysis were performed: a search for coincidences with a low background of
accidentals (0.1 over the entire period), and the calculation of upper limits
on the rate of gravitational wave bursts. Here we give a detailed account of
the methodology and we report the results: a null search for coincident events
and an upper limit that improves over all previous limits from resonant
antennas, and is competitive, in the range h_rss ~1E-19, with limits from
interferometric detectors. Some new methodological features are introduced that
have proven successful in the upper limits evaluation.Comment: 12 pages, 12 figure
Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors.
Abstract
This study examines the regulation of cGMP by illumination and by calcium during signal transduction in vertebrate retinal photoreceptor cells. We employed an electropermeabilized rod outer segment (EP-ROS) preparation which permits perfusion of low molecular weight compounds into the cytosol while retaining many of the features of physiologically competent, intact rod outer segments (ROS). When nucleotide-depleted EP-ROS were incubated with MgGTP, time- and dose-dependent increases in intracellular cGMP levels were observed. The steady state cGMP concentration in EP-ROS (0.007 mol cGMP per mol rhodopsin) approached the cGMP concentration in intact ROS. Flash illumination of EP-ROS in a 250-nM free calcium medium resulted in a transient decrease in cGMP levels; this occurred in the absence of changes in calcium concentration. The kinetics of the cGMP response to flash illumination of EP-ROS were similar to that of intact ROS. To further examine the effects of calcium on cGMP metabolism, dark-adapted EP-ROS were incubated with MgGTP containing various concentrations of calcium. We observed a twofold increase in cGMP steady state levels as the free calcium was lowered from 1 μM to 20 nM; this increase was comparable to the behavior of intact ROS. Measurements of guanylate cyclase activity in EP-ROS showed a 3.5-fold increase in activity over this range of calcium concentrations, indicating a retention of calcium regulation of guanylate cyclase in EP-ROS preparations. Flash illumination of EP-ROS in either a 50- or 250-nM free calcium medium revealed a slowing of the recovery time course at the lower calcium concentration. This observation conflicts with any hypothesis whereby a reduction in free calcium concentration hastens the recovery of cytoplasmic cGMP levels, either by stimulating guanylate cyclase activity or by inhibiting phosphodiesterase activity. We conclude that changes in the intracellular calcium concentration during visual transduction may have more complex effects on the recovery of the photoresponse than can be accounted for solely by guanylate cyclase activation
Variable binding, symmetric monoidal closed theories, and bigraphs
This paper investigates the use of symmetric monoidal closed (SMC) structure
for representing syntax with variable binding, in particular for languages with
linear aspects. In our setting, one first specifies an SMC theory T, which may
express binding operations, in a way reminiscent from higher-order abstract
syntax. This theory generates an SMC category S(T) whose morphisms are, in a
sense, terms in the desired syntax. We apply our approach to Jensen and
Milner's (abstract binding) bigraphs, which are linear w.r.t. processes. This
leads to an alternative category of bigraphs, which we compare to the original.Comment: An introduction to two more technical previous preprints. Accepted at
Concur '0
Effect of cosmic rays on the resonant gravitational wave detector NAUTILUS at temperature T=1.5 K
The interaction between cosmic rays and the gravitational wave bar detector
NAUTILUS is experimentally studied with the aluminum bar at temperature of
T=1.5 K. The results are compared with those obtained in the previous runs when
the bar was at T=0.14 K. The results of the run at T = 1.5 K are in agreement
with the thermo-acoustic model; no large signals at unexpected rate are
noticed, unlike the data taken in the run at T = 0.14 K. The observations
suggest a larger efficiency in the mechanism of conversion of the particle
energy into vibrational mode energy when the aluminum bar is in the
superconductive status.Comment: 7 pages, 3 figures, 2 tables. Accepted by Physics Letters
Vibrational excitation induced by electron beam and cosmic rays in normal and superconductive aluminum bars
We report new measurements of the acoustic excitation of an Al5056
superconductive bar when hit by an electron beam, in a previously unexplored
temperature range, down to 0.35 K. These data, analyzed together with previous
results of the RAP experiment obtained for T > 0.54 K, show a vibrational
response enhanced by a factor 4.9 with respect to that measured in the normal
state. This enhancement explains the anomalous large signals due to cosmic rays
previously detected in the NAUTILUS gravitational wave detector.Comment: 28 pages, 13 figure
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