MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

AbstractComplex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.Abstract Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15

The interplay of matrix metalloproteinase-8, transforming growth factor-beta 1 and vascular endothelial growth factor-C cooperatively contributes to the aggressiveness of oral tongue squamous cell carcinoma

Background: Matrix metalloproteinase-8 (MMP-8) has oncosuppressive properties in various cancers. We attempted to assess MMP-8 function in oral tongue squamous cell carcinoma (OTSCC). Methods: MMP-8 overexpressing OTSCC cells were used to study the effect of MMP-8 on proliferation, apoptosis, migration, invasion and gene and protein expression. Moreover, MMP-8 functions were assessed in the orthotopic mouse tongue cancer model and by immunohistochemistry in patient samples. Results: MMP-8 reduced the invasion and migration of OTSCC cells and decreased the expression of MMP-1, cathepsin-K and vascular endothelial growth factor-C (VEGF-C). VEGF-C was induced by transforming growth factor-beta 1 (TGF-beta 1) in control cells, but not in MMP-8 overexpressing cells. In human OTSCC samples, low MMP-8 in combination with high VEGF-C was an independent predictor of poor cancer-specific survival. TGF-beta 1 treatment also restored the migration of MMP-8 overexpressing cells to the level of control cells. In mouse tongue cancer, MMP-8 did not inhibit metastasis, possibly because it was eliminated in the peripheral carcinoma cells. Conclusions: The suppressive effects of MMP-8 in OTSCC may be mediated through interference of TGF-beta 1 and VEGF-C function and altered proteinase expression. Together, low MMP-8 and high VEGF-C expression have strong independent prognostic value in OTSCC.Peer reviewe

Propriedades Laboratoriais e Clínicas dos Compostos Cerâmicos nas Reabilitações Orais Estéticas: Revisão de Literatura

Atualmente, existe uma ampla busca para a reabilitação oral através de tratamentos estéticos, onde gerou um grande avanço tecnológico e científico, que permitiu o desenvolvimento de materiais e técnicas reabilitadores que buscam excelência nos procedimentos clínicos estéticos. Os compostos cerâmicos são materiais odontológicos com grande avanço e utilização efetiva na clínica odontológica, como exemplo as próteses livres de metal. Este trabalho tem como objetivo revisar a literatura mais atual sobre os compostos cerâmicos, suas propriedades, aspectos funcionais e estéticos. O levantamento bibliográfico foi realizado através de artigos científicos publicados do ano de 2008 até o ano de 2017 dos bancos de dados Scielo, Bireme e PubMed, utilizando os descritores Prótese Parcial Fixa, Estética e Cerâmica. Foram incluídos artigos que apresentavam dados pertinentes ao assunto proposto, após a leitura do Resumo de 30 artigos, foram selecionados 25 que apresentavam a temática em questão, sendo 5 artigos excluídos por não exibirem de forma precisa e direta o critério esperado, sendo este, uma abordagem atual sobre reabilitações orais estéticas. Diante dos artigos avaliados, pode-se observar que, os compostos cerâmicos permitem uma larga utilização em reabilitações orais com resultados clínicos bastante satisfatórios. Concluiu-se que devido aos grandes avanços tecnológicos e científicos nas estruturas cerâmicas, permitiram com que as reabilitações orais estéticas apontassem grande avanço em seu comportamento mecânico, biológico e estético.Palavras-chave: Estética. Cerâmica. Prótese Parcial Fixa.</jats:p

Aspectos Clínicos para a Realização do Procedimento de Moldagem de Trabalho em Prótese Parcial Fixa: Revisão de Literatura

A moldagem é um passo fundamental em um tratamento reabilitador protético oral, quanto melhor a exatidão deste procedimento, mais informações serão alcançadas, possibilitando assim, que se realize um trabalho com sucesso. O objetivo do trabalho é expor os achados na literatura sobre os aspectos clínicos dos materiais e técnicas de moldagem de trabalho em prótese parcial fixa. O levantamento bibliográfico foi realizado através de artigos científicos publicados a partir do ano de 2010 até o ano de 2016, dos bancos de dados SciELO, Bireme e PubMed, por meio dos descritores: Próteses, Impressão e Materiais. Após a leitura do Resumo de trinta artigos, foram selecionados vinte e cinco que apresentavam a temática em questão, sendo cinco artigos excluídos por    não apresentarem de forma concisa e direta o critério esperado, sendo este, uma abordagem dos materiais e técnicas de moldagem de trabalho em prótese parcial fixa. Com base nos artigos avaliados, pode-se observar que os elastômeros são os materiais mais utilizados na execução de moldagens de trabalho em prótese parcial fixa, e em relação as técnicas de moldagem, foi visto que todas as técnicas podem ser utilizadas, não apresentando diferença significativa entre elas. Contudo para se obter sucesso clínico neste procedimento, o mesmo deve ser criteriosamente executado, cabendo aos cirurgiões dentistas respeitar suas limitações e utilizar a técnica de forma precisa para que sua reabilitação oral alcance excelência. Palavras-chave: Próteses. Impressão. Materiais.</jats:p

MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated "Focal adhesion" and "ECM-receptor interaction" as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren't significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.Peer reviewe

Expression of S-100, EMA, CD34 and presence of mast cells in eight oral neurofibromas, and a review of 127 cases of the literature

INTRODUCTION: The rarity of oral neurofibromas (ONs) generates problems regarding their epidemiological and immunohistochemical characterization. OBJECTIVES: The aim of this study was to evaluate the expression of different markers in ONs and review epidemiologic data reported in the literature. MATERIAL AND METHODS: Clinicopathologic, immunohistochemical (markers S-100, epithelial membrane antigen [EMA], CD34) and histochemical (modified-Ziehl-Neelsen-method) studies were performed in eight cases of ON diagnosed in the Department of Pathology and Legal Medicine (DPML), Universidade Federal do Ceará (UFC), Ceará, Brazil, between 1994 and 2010. RESULTS: Oral neurofibromas represented 0.2% of the oral lesions diagnosed by our service in 16 years, and the buccal mucosa was the most frequent oral site (71.4%). Seven (87.5%) and 8 (100.0%) cases were positive for S-100 and CD34, respectively, and none for EMA. Mast cells were identified in seven cases (87.5%). The literature search indicated that solitary ONs are more common and occur preferentially in females, affecting patients between 30 and 40 years old. The alveolar ridge is the most commonly involved site. CONCLUSION: S-100- and CD34 markers proved to be of great value as a diagnostic tool, unlike EMA staining. Identification of mast cells in most cases suggests their involvement in this tumor pathogenesis. The clinicopathologic data retrieved from the literature enabled the establishment of a more consistent epidemiological profile