Age difference between heterosexual partners in Britain: Implications for the spread of Chlamydia trachomatis

Heterosexual partners often differ in age. Integrating realistic patterns of sexual mixing by age into dynamic transmission models has been challenging. The effects of these patterns on the transmission of sexually transmitted infections (STI) including Chlamydia trachomatis (chlamydia), the most common bacterial STI are not well understood. We describe age mixing between new heterosexual partners using age- and sex-specific data about sexual behavior reported by people aged 16-63 years in the 2000 and 2010 British National Surveys of Sexual Attitudes and Lifestyles. We incorporate mixing patterns into a compartmental transmission model fitted to age- and sex-specific, chlamydia positivity from the same surveys, to investigate C. trachomatis transmission. We show that distributions of ages of new sex partners reported by women and by men in Britain are not consistent with each other. After balancing these distributions, new heterosexual partnerships tend to involve men who are older than women (median age difference 2, IQR -1, 5 years). We identified the most likely age combinations of heterosexual partners where incident C. trachomatis infections are generated. The model results show that in >50% of chlamydia transmitting partnerships, at least one partner is ≥25 years old. This study illustrates how sexual behavior data can be used to reconstruct detailed sexual mixing patterns by age, and how these patterns can be integrated into dynamic transmission models. The proposed framework can be extended to study the effects of age-dependent transmission on incidence in any STI

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

Caveolin and caveolin containing rafts are involved in the signaling of growth factors in various cell types. Previous reports of our lab indicated a co-localization of caveolin and the high affinity nerve growth factor (NGF) receptor tyrosine kinase A (TrkA). Mutual effects have been observed among which a caveolin-1 knock-down resulted in an impairment of the NGF signaling cascade rather than in an increase of activity as expected from other growth factor reports. On the other hand, an over-expression of caveolin-1 impaired the NGF stimulated activity of p42/44 mitogen activated protein kinases (MAPK). In this study, we used a caveolin-1 scaffolding domain (CSD) peptide (cavtratin) of which an inhibitory effect on growth factor receptors was reported. Our data showed that cavtratin suppresses the NGF-induced phosphorylation of TrkA as well as the activation of MAPK in porcine oligodendrocytes significantly

Phosphocholine – an agonist of metabotropic but not of ionotropic functions of alpha9-containing nicotinic acetylcholine receptors

We demonstrated previously that phosphocholine and phosphocholine-modified macromolecules efficiently inhibit ATP-dependent release of interleukin-1beta from human and murine monocytes by a mechanism involving nicotinic acetylcholine receptors (nAChR). Interleukin-1beta is a potent pro-inflammatory cytokine of innate immunity that plays pivotal roles in host defence. Control of interleukin-1beta release is vital as excessively high systemic levels cause life threatening inflammatory diseases. In spite of its structural similarity to acetylcholine, there are no other reports on interactions of phosphocholine with nAChR. In this study, we demonstrate that phosphocholine inhibits ion-channel function of ATP receptor P2X7 in monocytic cells via nAChR containing alpha9 and alpha10 subunits. In stark contrast to choline, phosphocholine does not evoke ion current responses in Xenopus laevis oocytes, which heterologously express functional homomeric nAChR composed of alpha9 subunits or heteromeric receptors containing alpha9 and alpha10 subunits. Preincubation of these oocytes with phosphocholine, however, attenuated choline-induced ion current changes, suggesting that phosphocholine may act as a silent agonist. We conclude that phophocholine activates immuno-modulatory nAChR expressed by monocytes but does not stimulate canonical ionotropic receptor functions

Anatomical organization of the cerebrum of the praying mantis <em>Hierodula membranacea</em>

\ua9 2024 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC.Many predatory animals, such as the praying mantis, use vision for prey detection and capture. Mantises are known in particular for their capability to estimate distances to prey by stereoscopic vision. While the initial visual processing centers have been extensively documented, we lack knowledge on the architecture of central brain regions, pivotal for sensory motor transformation and higher brain functions. To close this gap, we provide a three-dimensional (3D) reconstruction of the central brain of the Asian mantis, Hierodula membranacea. The atlas facilitates in-depth analysis of neuron ramification regions and aides in elucidating potential neuronal pathways. We integrated seven 3D-reconstructed visual interneurons into the atlas. In total, 42 distinct neuropils of the cerebrum were reconstructed based on synapsin-immunolabeled whole-mount brains. Backfills from the antenna and maxillary palps, as well as immunolabeling of γ-aminobutyric acid (GABA) and tyrosine hydroxylase (TH), further substantiate the identification and boundaries of brain areas. The composition and internal organization of the neuropils were compared to the anatomical organization of the brain of the fruit fly (Drosophila melanogaster) and the two available brain atlases of Polyneoptera—the desert locust (Schistocerca gregaria) and the Madeira cockroach (Rhyparobia maderae). This study paves the way for detailed analyses of neuronal circuitry and promotes cross-species brain comparisons. We discuss differences in brain organization between holometabolous and polyneopteran insects. Identification of ramification sites of the visual neurons integrated into the atlas supports previous claims about homologous structures in the optic lobes of flies and mantises

Importance of tides for periastron precession in eccentric neutron star-white dwarf binaries

Although not nearly as numerous as binaries with two white dwarfs, eccentric neutron star-white dwarf (NS-WD) binaries are important gravitational-wave (GW) sources for the next generation of space-based detectors sensitive to low frequency waves. Here we investigate periastron precession in these sources as a result of general relativistic, tidal, and rotational effects; such precession is expected to be detectable for at least some of the detected binaries of this type. Currently, two eccentric NS-WD binaries are known in the galactic field, PSR J1141-6545 and PSR B2303+46, both of which have orbits too wide to be relevant in their current state to GW observations. However, population synthesis studies predict the existence of a significant Galactic population of such systems. Though small in most of these systems, we find that tidally induced periastron precession becomes important when tides contribute to more than 3% of the total precession rate. For these systems, accounting for tides when analyzing periastron precession rate measurements can improve estimates of the inferred WD component mass and, in some cases, will prevent us from misclassifying the object. However, such systems are rare, due to rapid orbital decay. To aid the inclusion of tidal effects when using periastron precession as a mass measurement tool, we derive a function that relates the WD radius and periastron precession constant to the WD mass.Facultad de Ciencias Astronómicas y Geofísica

LAMINITE EM PORCAS DESCARTADAS PROVENIENTES DE GRANJAS COMERCIAIS DE SUÍNOS DO SUL DO BRASIL

Although laminitis has been reported in pigs, distal phalanx rotation detected by radiography has not been described yet in this species. Accordingly, this paper describes three cases of laminitis in sows from a Brazilian commercial swine unit, and a preliminary study on laminitis in culled sows from a slaughterhouse, based on radiographic evaluation. A farrow-to-finish unit located in a central swine production area in southern Brazil showed a history of locomotor alterations in sows. Three lame sows were selected for examination. They displayed typical clinical signs of laminitis. Their feet were radiographed, and all three showed distal phalanx deviation in at least one digit. In order to find out the possibility of laminitis occurrence in other farms, thirteen hind and five front feet from lame sows were collected in a slaughterhouse, and radiographed in lateromedial position. Eleven sows (61%) showed distal phalanx rotation of the third or the fourth digit, whereas eight of them (44%) showed this alteration in both digits. Osteolysis in five sows (28%) and periosteal proliferation in another four sows (11%) were observed. In conclusion, laminitis with distal phalanx rotation is present in Brazilian herds, and radiographic analysis may be an important diagnosis tool for the disease in vivo or at slaughterhouses.Embora a laminite tenha sido relatada em suínos, a rotação de falange distal detectada por radiografia ainda não foi descrita nessa espécie. O presente trabalho descreve três casos de laminite em porcas provenientes de uma granja comercial de suínos bem como relata um estudo preliminar sobre laminite em porcas de descarte em abatedouro, baseado em avaliações radiográficas. Uma granja de ciclo completo localizada em uma área de exploração suinícola do sul do Brasil mostrou histórico de alterações locomotoras em porcas. Três porcas com claudicação foram selecionadas para exame. Elas apresentavam sinais clínicos típicos de laminite. Seus membros foram radiografados e todas apresentaram rotação de falange distal em pelo menos um dígito. Para verificar a presença de laminite em outros rebanhos da região, treze membros pélvico e cinco membros torácicos de matrizes suínas foram colhidos em um abatedouro e radiografados em posição latero-medial. Onze porcas (61%) mostraram rotação de falange distal no terceiro ou no quarto dígito, enquanto oito delas (44%) mostraram essa alteração em ambos os dígitos. Foram observadas osteólises em cinco (28%) porcas e proliferação periosteal em quatro porcas (11%). Em conclusão, a laminite está presente em rebanhos suínos brasileiros, e a análise radiográfica constitui-se em uma importante ferramenta para o diagnóstico da doença in vivo ou em abatedouros

Effect of biochar on the mirobial enzymes activity of soil with Eucalyptus.

Session 5- Bacterial and Plant Physiology - Poster V.5

Evolutionary and pulsational properties of white dwarf stars

Abridged. White dwarf stars are the final evolutionary stage of the vast majority of stars, including our Sun. The study of white dwarfs has potential applications to different fields of astrophysics. In particular, they can be used as independent reliable cosmic clocks, and can also provide valuable information about the fundamental parameters of a wide variety of stellar populations, like our Galaxy and open and globular clusters. In addition, the high densities and temperatures characterizing white dwarfs allow to use these stars as cosmic laboratories for studying physical processes under extreme conditions that cannot be achieved in terrestrial laboratories. They can be used to constrain fundamental properties of elementary particles such as axions and neutrinos, and to study problems related to the variation of fundamental constants. In this work, we review the essentials of the physics of white dwarf stars. Special emphasis is placed on the physical processes that lead to the formation of white dwarfs as well as on the different energy sources and processes responsible for chemical abundance changes that occur along their evolution. Moreover, in the course of their lives, white dwarfs cross different pulsational instability strips. The existence of these instability strips provides astronomers with an unique opportunity to peer into their internal structure that would otherwise remain hidden from observers. We will show that this allows to measure with unprecedented precision the stellar masses and to infer their envelope thicknesses, to probe the core chemical stratification, and to detect rotation rates and magnetic fields. Consequently, in this work, we also review the pulsational properties of white dwarfs and the most recent applications of white dwarf asteroseismology.Comment: 85 pages, 28 figures. To be published in The Astronomy and Astrophysics Revie

Movement of a secondary immiscible liquid in a suspension using a non-invasive technique

In this paper, the movement of a secondary immiscible liquid when added to a suspension of hydrophilic particles in a continuous hydrophobic phase is investigated. This was achieved through an approach using high speed camera and X-ray computer tomography. These non-invasive approaches allowed the secondary liquid displacement within the suspension to be monitored on the surface level and within the suspension through a time lapse of scans. The addition of a small amount of secondary liquid to suspensions, can lead to a transition from a fluid-like to paste-like structure. The kinetics taking place and responsible for this, during both short and long term storage were investigated to better understand the mechanisms taking place. Water was added as the secondary immiscible liquid to suspensions composed of sucrose (icing sugar) and sunflower oil. Different volumes of secondary liquid were added to the suspensions. The rate of movement as well as the spreading of the secondary liquid into the suspension was calculated from the scans taken. The surface area to volume ratio was proposed as a reason for the spreading of the liquid for the smaller volume droplet being greater in comparison to the larger volume droplet

Bidirectional transfer study of polystyrene nanoparticles across the placental barrier in an ex vivo human placental perfusion model

BACKGROUND: Nanoparticle exposure in utero might not be a major concern yet, but it could become more important with the increasing application of nanomaterials in consumer and medical products. Several epidemiologic and in vitro studies have shown that nanoparticles can have potential toxic effects. However, nanoparticles also offer the opportunity to develop new therapeutic strategies to treat specifically either the pregnant mother or the fetus. Previous studies mainly addressed whether nanoparticles are able to cross the placental barrier. However, the transport mechanisms underlying nanoparticle translocation across the placenta are still unknown. OBJECTIVES: In this study we examined which transport mechanisms underlie the placental transfer of nanoparticles. METHODS: We used the ex vivo human placental perfusion model to analyze the bidirectional transfer of plain and carboxylate modified polystyrene particles in a size range between 50 and 300 nm. RESULTS: We observed that the transport of polystyrene particles in the fetal to maternal direction was significantly higher than for the maternal to fetal direction. Regardless of their ability to cross the placental barrier and the direction of perfusion, all polystyrene particles accumulated in the syncytiotrophoblast of the placental tissue. CONCLUSIONS: Our results indicate that the syncytiotrophoblast is the key player in regulating nanoparticle transport across the human placenta. The main mechanism underlying this translocation is not based on passive diffusion, but is likely to involve an active, energy-dependent transport pathway. These findings will be important for reproductive toxicology as well as for pharmaceutical engineering of new drug carriers. CITATION: Grafmueller S, Manser P, Diener L, Diener PA, Maeder-Althaus X, Maurizi L, Jochum W, Krug HF, Buerki-Thurnherr T, von Mandach U, Wick P. 2015. Bidirectional transfer study of polystyrene nanoparticles across the placental barrier in an ex vivo human placental perfusion model. Environ Health Perspect 123:1280-1286; http://dx.doi.org/10.1289/ehp.1409271