USE OF SALICYLIC ACID IN LETTUCE PLANT AGAINST INCREASING METEOROLOGICAL DROUGHT

Climate change and related meteorological drought have been one of the most important factors limiting plant production in Turkey as well as in the whole world. Producers have to develop quick solutions for sudden drought and water shortage conditions in the field. One of these solutions is the application of various chemicals that provide resistance to drought during the growing period of plants. Since the Southeastern Anatolia Region of Türkiye is a semi-arid region, mostly irrigated agriculture is carried out from early spring to the beginning of winter. Especially in dry periods, the negative effects of drought are seen rapidly in plants such as lettuce, which are sensitive to water deficiency. In order to reduce these negative effects, different salicylic acid (SA) concentrations were tried under deficient irrigation conditions in lettuce plant grown in autumn planting in Diyarbakır. For this purpose, three different irrigation water amounts and four different SA concentrations were applied. According to these applications, the lowest leaf length was obtained from the subject where SA was not applied and the lowest dose of irrigation water. The lowest leaf width was obtained from the subject where the lowest dose of irrigation water was applied. The highest whole plant dry weight was obtained from the subject treated with the least amount of water and 0.50 mM SA. The effects of irrigation water and SA on SPAD readings and the water-soluble dry matter parameter in plants were not statistically significant

Assessing the Effect of Piperacillin/Tazobactam on Hematological Parameters in Patients Admitted with Moderate or Severe Foot Infections

Introduction: Piperacillin/tazobactam is a commonly used antibiotic for the empirical treatment of severe diabetic foot infections. One of the most feared complications of this drug is the development of pancytopenia. The aim of this study was to determine whether the use of piperacillin/tazobactam caused any hematological changes in patients admitted with severe diabetes-related foot infections from a specialist multidisciplinary foot clinic. Specifically, looking at whether it caused anemia, leukopenia, neutropenia, or thrombocytopenia. Methods: A 1-year retrospective analysis of patients admitted to a tertiary care center for treatment of diabetes-related foot infection using piperacillin/tazobactam. Hematological indices, urea and electrolytes, and C-reactive protein (CRP) were recorded pretreatment, during treatment, and posttreatment. HbA1c, vitamin B12, folate, thyroid-stimulating hormone, and free thyroxin were also analyzed to exclude any potential confounders as a cause of pancytopenia. Results: A total of 154 patients were admitted between 1 January 2016 and 31 December 2016 who received piperacillin/tazobactam for severe diabetes-related foot infection. On admission, white cell count and CRP were raised and fell significantly within the first 48 h. Other hematological factors did not change. Five patients developed a mild pancytopenia, of which three were unexplained. Conclusion: In this relatively small cohort, pancytopenia did not occur. As such, piperacillin/tazobactam appeared to have a low risk of adverse hematological outcomes and remains the treatment of choice for severe diabetes-related foot infections

Effects of domestic wastewater treated by anaerobic stabilization on soil pollution, plant nutrition, and cotton crop yield

PubMedID: 27837365This study has aimed to determine the effects of treated wastewater on cotton yield and soil pollution in Southeastern Anatolia Region of Turkey during 2011 and 2012. The treated wastewater was provided from the reservoir operated as anaerobic stabilization. After treatment, suspended solids (28–60 mg/l), biological oxygen demand (29–30 mg/l), and chemical oxygen demand (71–112 mg/l) decreased significantly compared to those in the wastewater. There was no heavy metal pollution in the water used. There were no significant amounts of coliform bacteria, fecal coliform, and Escherichia coli compared to untreated wastewater. The cottonseed yield (31.8 g/plant) in the tanks where no commercial fertilizers were applied was considerably higher compared to the yield (17.2 g/plant) in the fertilized tanks where a common nitrogenous fertilizer was utilized. There were no significant differences between the values of soil pH. Soil electrical conductivity (EC) after the experiment increased from 0.8–1.0 to 0.9–1.8 dS/m. Heavy metal pollution did not occur in the soil and plants, because there were no heavy metals in the treated wastewater. It can be concluded that treated domestic wastewater could be used to grow in a controlled manner crops, such as cotton, that would not be used directly as human nutrients. © 2016, Springer International Publishing Switzerland

Correlation of nurses' mindfulness with their perception of individualized care: A cross-sectional study

Purpose The objective of this study is to investigate the correlation of nurses' mindfulness with their perception of individualized care. Design and Methods It was designed as a descriptive correlational study. Two hundred seventy-one nurses were included in this study. Findings The mean total score was 4.20 +/- 0.04 on the Mindful Attention Awareness Scale and 3.97 +/- 0.04 on the Individualized Care Scale-Nurse. There was a significant positive correlation between the mean total score on the scales and the subscales (p < 0.01). Practical Implications Therefore, the education and practical training of nurses should include methods designed to improve their levels of mindfulness

PIONEERING in vitro STUDIES FOR CALLUS FORMATION OF Colchicum chalcedonicum Azn.

Colchicum calcedonicum Azn is one of the endemic species distributed in Turkey, where many endemic plant species occur. It has long-oval shaped corm under the soil, and usually 3-4 leaves on it. In vitro production of endemic species using callus culture has become promising study for conservation. The aim of this study is to generate an efficient callus protocol for in vitro production of C. chalcedonicum. To sterilize the explants, 0.25% (w/v) mercuric chloride (HgCl2) was used for 20 min. In addition to mercuric chloride, surface sterilization was conducted by using 6.5% NaCl with Tween 80 for 30 min. We used 19 different mediums and the primary callus formation was obtained in Murashige & Skoog's basal medium (MS) supplemented with 2,4-D (2 mg L-1), 2IP (0.5 mg L-1), 3% sucrose and 0.05% active carbon. Our study demonstrated the active carbon usage was effective for the primary callus formation. This study is the first report for primary callus formation of C. chalcedonicum. However, our work is a pioneering study to improve callus formation protocol system for in vitro conservation of endemic species C. chalcedonicum

In vitro propagation of Amsonia orientalis Decne (Apocynaceae)

In vitro plant regeneration was achieved from shoot explants of Amsonia orientalis Decne. The seeds were germinated aseptically in Petri dishes containing growth-regulator-free MS medium. The shoot explants of 30-day-old grown seedlings were cultured on MS medium supplemented with 1 mg/l indole-3-butryric acid (IBA) plus 0.5 mg/l kinetin (Kin) for direct regeneration and subcultured at three week intervals. While induction of adventitious buds from shoot explants was achieved approximately 33% within four weeks, a few roots (around 15%) were induced on the same medium within eight weeks. In addition, shoot explants were transferred to MS medium containing 2 mg/l benzyl aminopurine (BAP) plus 0.2 mg/l naphtalene acetic acid (NAA) for indirect regeneration and after three weeks, proliferated actively growing calli was occured

Effects of intercropping on plant nutrient uptake in various vegetables species

The objective of this study was to determine the effect of intercropping system on some vegetables nutrient uptake in Adana ecological conditions. The experimental research was conducted under field conditions and at the plant physiology laboratory of the Department of Horticulture, Faculty of Agriculture of Cukurova University during 2003-2005. In the first year of this work, cauliflower and broccoli, in the second year cauliflower, cabbage and lettuce were used as main crop. Lettuce, pea, leek, garlic and onion were used as intercrops in the first year; green bean, pea, radish, leek, garlic and onion were used as intercrops in the second year. In general, it can be said that the vegetables cropped solely uptaked more mineral contents while for those grown by intercropping and competitions were determined in intercropped systems

Characterization of peripheral blood T follicular helper (TFH) cells in patients with type 1 Gaucher disease and carriers

© 2023 Elsevier Inc.Background: Gaucher disease (GD) is the most common autosomal recessive lipid storage disease. In this study, the changes in TFH cells and IL-4 and IL-21 cytokines in blood samples of GD patients, carriers and healthy volunteers were investigated. Methods: Two pretreatment type 1 GD patients, 20 currently treated type 1 GD patients, 6 carriers, and 27 healthy volunteers were enrolled in the study. TFH cell (CD45RA−CD4+CXCR5+) number, phenotype (PD1, ICOS expression), and cytokine production (IL-21, IL-4) were assessed via flow cytometric assays. Results: No significant differences were found between the groups with respect to the number, frequency and PD1 or ICOS expression of TFH cells between healthy controls, patients and carriers. However, IL-4+ TFH cells were significantly reduced both in percent and number in the treated GD patients compared with healthy controls (p < 0.05). Interestingly, the IL-21+ TFH cell number was increased in treated GD patients. When TFH cells were examined based on CXCR3 expression, the frequency of the PD1+Th17-Th2-like fraction (CXCR3−) was found to be significantly increased in treated GD patients. Conclusion: To our knowledge, this is the first study to assess TFH cells in GD patients, and to show that the production of IL-4 and IL-21 by TFH cells and their subsets may be altered in type 1 GD patients