Improvement of the quality of higher school training of future vocational teachers on the basis of application of IT-technologies

The article deals with the possible use of IT-technologies for improvement of quality of future vocational teachers training in the formation of a new educational system.The experience of the development of the electronic textbook for the discipline «Devices and automats for precision and quality control» is presentedАнализируются возможности использования IT-технологий для повышения качества подготовки будущих педагогов профессионального обучения. Представлен опыт разработки электронного учебного пособия по дисциплине «Приборы и автоматы для контроля точности и качества

NK cells expansion <i>in vitro</i> is followed by loss of inhibitory KIR expression

NK cells are innate lymphocytes that are able to eliminate altered cells, which makes them promising for the immunotherapy of viral diseases and tumors. The NK cell population is characterized by high phenotypic and functional diversity. In particular, in the pool of highly differentiated NK cells in the presence of cytomegalovirus (HCMV), a population of adaptive cells can be formed, characterized by a high lifespan and high cytotoxicity. However, in order to carry out a cytotoxic reaction, a NK cell must undergo a licensing process, during which it acquires the expression of NKG2A and KIRs. Currently, there are many effective methods of NK cell accumulation for subsequent use in therapy, one of them is the stimulation with IL-2 and K562-mbIL21 feeder cells. Highly differentiated adaptive-like NK cells are able to expand in respond to such stimulation. However, the phenotype of actively expanding NK cells dynamically changes. Loss of inhibitory KIR expression during intense proliferation of NK cells may adversely affect their cytotoxic potential. This work shows that highly differentiated CD56dimNKG2C+ NK cells from HCMV-seropositive individuals have a high proportion of KIR2DL2/3+ cells. This may indicate a high stability of KIR receptor expression in this population. We have shown that CD56dimNKG2C+ clonal cultures obtained by stimulation with IL-2 and K562- mbIL21 are characterized by high stability of KIR2DL2/3 expression compared to NKG2C-negative and less differentiated CD56brightNKG2C+. Also, in heterogeneous cultures of adaptive NK cells precursors CD57- CD56dimNKG2C+, a higher expression level of KIR2DL2/3 was observed in comparison with NKG2C-negative cultures of CD57-CD56dimNKG2C-. Thus, the accumulation of NK cells upon stimulation with IL-2 and K562- mbIL2 feeder cells can lead to loss of expression of KIR receptors and a decrease in their functional activity. However, cultures of highly differentiated NK cells of HCMV-seropositive individuals CD56dimNKG2C+, as well as cultures of precursors of adaptive NK cells CD57-CD56dimNKG2C+, are characterized by a greater stability of KIR2DL2/3 expression. As a result, stimulation with IL-2 and K562-mbIL21 feeder cells can be used to accumulate adaptive-like cells and their progenitors with stable inhibitory KIR expression and high cytotoxic potential

The use of distance learning technologies in teaching of "Outpatient and emergency pediatrics"

The purpose of the study is to evaluate customer satisfaction in using of distance learning technologies in teaching of 'outpatient and emergency pediatrics'. In anonymous survey at the university educational portal 35 students of the 5th course of pediatric faculty were attended. Educational technology, using new possibilities of telecommunication systems shown their efficiency in the development of the necessary competencies, they are actual and meet the demands of consumers of educational services.Цель исследования - оценка удовлетворенности потребителей образовательных услуг при использовании дистанционных образовательных технологий в ходе освоения дисциплины «Поликлиническая и неотложная педиатрия». В анонимном опросе на университетском образовательном портале приняло участие 35 студентов 5 курса педиатрического факультета. Образовательные технологии, использующие новые возможности телекоммуникационных систем, показали свою эффективность при освоении необходимых компетенций, современны и соответствуют запросам потребителей образовательных услуг

A COMPARATIVE STUDY OF OPTICAL PROPERTIES OF BENZOTHIENOACENE-BASED SOLUTIONS, THIN FILMS, AND POWDERS

The investigation of novel organic materials for the production of semiconductor devices is a topic of significant interest in the flexible electronics. The objective of this study is to determine the optical properties of benzothienoacene-based materials in dissolved, thin film, and powdered forms.Исследование выполнено при финансовой поддержке Министерства науки и высшего образования Российской Федерации в рамках Программы развития Уральского федерального университета имени первого Президента России Б.Н. Ельцина в соответствии с программой стратегического академического лидерства «Приоритет-2030»

Fucans, but Not Fucomannoglucuronans, Determine the Biological Activities of Sulfated Polysaccharides from Laminaria saccharina Brown Seaweed

Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characterize, using in vitro and in vivo strategies, the anti-inflammatory, anti-coagulant, anti-angiogenic, and anti-tumor activities of two main sulfated polysaccharide fractions obtained from L. saccharina: a) L.s.-1.0 fraction mainly consisting of O-sulfated mannoglucuronofucans and b) L.s.-1.25 fraction mainly composed of sulfated fucans. Both fractions inhibited leukocyte recruitment in a model of inflammation in rats, although L.s.-1.25 appeared to be more active than L.s.-1.0. Also, these fractions inhibited neutrophil adhesion to platelets under flow. Only fraction L.s.-1.25, but not L.s.-1.0, displayed anticoagulant activity as measured by the activated partial thromboplastin time. Investigation of these fractions in angiogenesis settings revealed that only L.s.-1.25 strongly inhibited fetal bovine serum (FBS) induced in vitro tubulogenesis. This effect correlated with a reduction in plasminogen activator inhibitor-1 (PAI-1) levels in L.s.-1.25-treated endothelial cells. Furthermore, only parent sulfated polysaccharides from L. saccharina (L.s.-P) and its fraction L.s.-1.25 were powerful inhibitors of basic fibroblast growth factor (bFGF) induced pathways. Consistently, the L.s.-1.25 fraction as well as L.s.-P successfully interfered with fibroblast binding to human bFGF. The incorporation of L.s.-P or L.s.-1.25, but not L.s.-1.0 into Matrigel plugs containing melanoma cells induced a significant reduction in hemoglobin content as well in the frequency of tumor-associated blood vessels. Moreover, i.p. administrations of L.s.-1.25, as well as L.s.-P, but not L.s.-1.0, resulted in a significant reduction of tumor growth when inoculated into syngeneic mice. Finally, L.s.-1.25 markedly inhibited breast cancer cell adhesion to human platelet-coated surfaces. Thus, sulfated fucans are mainly responsible for the anti-inflammatory, anticoagulant, antiangiogenic, and antitumor activities of sulfated polysaccharides from L. saccharina brown seaweed

Optical Properties of Benzothienoacenes

The study of the properties of thin films to produce semiconductor devises is of great interest in the field of flexible electronics. The purpose of this work is to determine the optical properties of raw materials based on benzothienoacenes in dissolved and powdered form

Клинический случай идиопатического легочного фиброза на фоне коморбидной патологии

Idiopathic pulmonary fibrosis (IPF) is one of the most common diseases in the group of interstitial lung diseases, which is characterized by persistent progression and poor prognosis. Over the past decade, experts have made significant progress in developing a diagnostic algorithm for IPF patients. This algorithm includes analysis of clinical, laboratory, and instrumental data, primarily the results of high-resolution computed tomography (HRCT). Precise adherence to the diagnostic algorithm and correct interpretation of HRCT data are prerequisites for IPF diagnosis.Specialists of the Tomsk region have developed routing of patients with suspected IPF. The presented clinical case is a successful example of adhering to this algorithm. Wide implementation of modern diagnostic algorithms into diagnosis and treatment of IPF and quality improvement of imaging methods, primarily HRCT, carried out as a part of the differential diagnosis, open up prospects for early diagnosis of this pathology. A timely prescribed antifibrotic therapy (nintedanib, pirfenidone) in IPF allows to slow down pathological progression and improves the prognosis.Идиопатический легочный фиброз (ИЛФ) относится к наиболее распространенным заболеваниям из группы интерстициальных заболеваний легких и характеризуется неуклонным прогрессированием и неблагоприятным прогнозом. В течение последнего десятилетия был достигнут значительный прогресс в разработке диагностического алгоритма для пациентов с ИЛФ, предполагающий анализ клинических, лабораторных и инструментальных данных, прежде всего, результатов компьютерной томографии высокого разрешения (КТВР). Точное следование алгоритму диагностики и правильная интерпретация данных КТВР являются необходимым условием для постановки диагноза ИЛФ.В Томской области разработана маршрутизация больных с подозрением на ИЛФ. Примером успешного следования этому алгоритму является представленный клинический случай. Широкое внедрение в лечебно-диагностический процесс современных алгоритмов диагностики ИЛФ и повышение качества визуализационных методов, прежде всего КТВР, проводимые в рамках дифференциального диагноза, открывают перспективы ранней диагностики данного патологического процесса, а своевременно назначенная антифибротическая терапия (нинтеданиб, пирфенидон) при ИЛФ позволяет замедлить прогрессирование патологического процесса и улучшить прогноз

Диагностика неатрофического антрального хеликобактер-ассоциированного гастрита у больных язвенной болезнью двенадцатиперстной кишки в сочетании с бронхиальной астмой с помощью тест-системы «Гастропанель»

This paper presents the results obtained by the investigation of morphological condition of a gastric mucosa and serum concentration of pepsinogen-I, pepsinogen-II, and gastrin-17, as well as H. pylori IgG antibodies using «Gastropanel» test system when examining patients suffering from a duodenal ulcer combined with bronchial asthma. It was reveled that the level of serum concentration is in close relation to gastritis activity and Helicjbacter pylori bacterization in the gastric mucosa, thus expanding the «Gastropanel» test system application for diagnosing not only atrophic gastritis, but also for helicobacter gastritis without evidence of atrophy.В статье представлены результаты изучения морфологического состояния слизистой оболочки желудка и сывороточных концентраций пепсиногена-I, пепсиногена-II, гастрина-17 и антител к Helicobacter pylori с использованием тест-системы «Гастропанель» у больных язвенной болезнью в сочетании с бронхиальной астмой. Выявлена взаимосвязь уровня сывороточных показателей с активностью гастрита и степенью обсемененности Helicobacter pylori слизистой оболочки желудка, что позволяет расширить область применения данной тест-системы для диагностики у больных не только атрофического гастрита, но и хеликобактерного гастрита без признаков атрофии