Postkomunističke novotvorine u istočnoj Evropi

Prijevod: Ivan Glaser

The Motiti decision: Implications for coastal management

The recent Court of Appeal decision in Attorney-General v The Trustees of the Motiti Rohe Moana Trust ([2019] NZCA 532) has wide-ranging implications for managing the sustained pressures on biodiversity in coastal ecosystems. The Motiti decision signals a profound change in the way in which regional councils will need to manage the territorial sea. In this article, I explore some of the implications of the decision for environmental management, in light of the recent public notice of decisions on the proposed Marlborough Environment Plan (MEP) by the Marlborough District Council on 21 February 2020

Identification of genes regulated by cold temperature - a study of mdtJI in E. coli O157:H7

E. coli O157:H7, a serious food-borne pathogen, is capable of adapting to distinctly different environments, ranging from the ruminant, to soil and water. Critical to the success of this pathogen, is the ability to adapt rapidly to changes in the environment. These changes rarely occur in isolation and bacteria, through regulatory networks, can respond to multiple challenges simultaneously, often through master regulators. Understanding the adaptive process of E. coli O157:H7, particularly in response to cold temperatures, is vital for elucidating the pathogens ability to persist during food processing. Of major concern to the meat industry, is the ability of E. coli O157:H7 to survive multiple hurdle intervention strategies that include both chilling and freezing. The aim of this project was to identify genes involved in the cold shock response of E. coli O157:H7 when exposed to refrigeration temperatures (4, 0, -1.5 C). We hypothesized that E. coli O157:H7 is able to withstand chill temperatures by up-regulating genes that allow survival in unfavorable conditions, for example, when the cell is expelled from the ruminant host, into soil or water environments. It is likely that E. coli O157:H7, utilizing similar adaptive mechanisms can withstand prolonged periods at refrigeration temperatures. Furthermore, we speculated that quorum sensing (QS) has overtime become integrated into these adaptive pathways, potentially forming an integrated component of the E. coli O157:H7 adaptive stress response, including the cold shock response. A number of genes were identified as being up-regulated in E. coli O157:H7 during incubation at 4 C on meat. Of these, four were of particular interest, as they had been previously linked to cell survival processes: slp (carbon starvation lipoprotein), hslJ (heat inducible protein), mdtI (multidrug efflux pump) and mdtJ (multidrug efflux pump). RT-PCR data showed that slp and the mdtJI complex are expressed more at refrigeration temperatures than at 37 C while hslJ expression was greatest at 37 C. mdtJI was selected for further analysis, because mdtJI was the only gene that was not expressed at 37 C when grown on BHI media, plus these genes had, at the initiation of this project, not been annotated or assigned function. Using a luxCDABE promoter reporter, real time analysis of the effect of temperature downshift on mdtJI expression was confirmed. Furthermore, expression was demonstrated to increase at high cell density at 37 C, suggesting a regulatory connection to quorum sensing. This coupled with the finding that 400nt upstream of the mdtJI promoter was a gene encoding a transporter of AI-2, a QS autoinducer, suggested a link to the LuxS/AI-2 QS regulatory network. Data presented here was unable to confirm a regulatory link to AI-2 itself but it did reveal a link to LuxS. In conclusion, data presented in this thesis has confirmed that mdtJI is involved in the adaptive response, specifically adaptation to cold temperatures in E. coli O157:H7, and possibly, to growth cessation. The influence of LuxS on mdtJI expression in E. coli O157:H7 is most likely to be through metabolic activity, rather than via a QS mechanism

Pomiędzy „religijną konfesyjnością” a „społeczną różnorodnością”. O przyszłości edukacji religijnej w Polsce

In recent years, Polish society has observed an intensification of changes in socio-religious conditions. In a special way, they have become the ‘hallmark’ of young Poles, who already exist uniquely between ‘religious denomination’ and ‘social diversity.’ The current form of Religious Education (school catechesis) is therefore becoming less and less compatible with the daily life of youth and will thus undoubtedly be difficult to maintain in the future in an unchanged form. In this context, this article – anchoredin a critical analysis of the present – is an attempt to ‘explore’ the future of Religious Education in Polish schools. To begin with, the authors present the symptoms of ‘social exhaustion’ concerning school catechesis and then focus on presenting ‘dialogue’ asa religious-pedagogical path of the future. The last paragraph is devoted to conceptual dilemmas, which include not only tasks related to the future teaching of religion, but also present guidelines related to the search for a future model of Religious Educationin Poland.Na przestrzeni ostatnich lat obserwuje się w polskim społeczeństwie intensyfikację przemian zachodzących w obrębie uwarunkowań społeczno-religijnych. W sposób szczególny stały się one „znakiem rozpoznawczym” młodych Polaków, którzy już dziś, w pewien sposób, egzystują pomiędzy „religijną konfesyjnością” a „społeczną różnorodnością”. Obecna forma edukacji religijnej (katecheza szkolna) staje się zatem coraz mniej kompatybilna z codziennością młodych ludzi i tym samym w niezmienionej formie będzie w przyszłości bez wątpienia trudna do utrzymania. W tym kontekście celem niniejszego artykułu jest – zakotwiczona w krytycznej analizie teraźniejszości – próba „wybiegnięcia” w przyszłość nauczania religii w polskich szkołach. W pierwszym kroku autorzy prezentują symptomy „wyczerpania” społecznego w odniesieniu do katechezy szkolnej, aby następnie skupić się na przedstawieniu „dialogu” jako ścieżki religijno-pedagogicznej przyszłości. Ostatni akapit poświęcony jest koncepcyjnym dylematom, które obejmują nie tylko zadania dotyczące nauczania religii w przyszłości, ale także prezentują wytyczne związane z poszukiwaniem przyszłościowego modelu edukacji religijnej w Polsce

Kilka uwag o roli naśladowania i uświadomienia w procesie pracy nad materiałem gramatycznym języka polskiego jako obcego

Zadanie pt. Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych przez Uniwersytet Łódzki nr 885/P-DUN/2014 zostało dofinansowane ze środków MNiSW w ramach działalności upowszechniającej naukę

Adolf Loos’ Villa For Dr Múller In Prague And Its Authenticity

[EN] This article is of great interest for two reasons: in the first place, it explains the restoration process on a unique building dating from the heroic time of the Modern Movement. This process followed a strict, painstaking methodology based on the concept of authenticity. In the second place, in a detailed analysis of his posthumous work, it reveals facets of Adolf Loos’s architectural beliefs never before discussed.[ES] Este artículo reúne un doble interés: en primer lugar relata el proceso de restauración de una obra singular de la época heroica del Movimiento Moderno, proceso que se ha basado en una metodología rigurosa y deliberada basada en el concepto de autenticidad; en segundo lugar desvela secretos hasta ahora soslayados sobre la propia concepción arquitectónica y espacial de Adolf Loos a través del análisis pormenorizado de su obra póstuma.Urlich, P. (2001). La villa del Dr. Müller en Praga y su autenticidad. Loggia, Arquitectura & Restauración. (12):52-65. doi:10.4995/loggia.2001.3607SWORD52651

Store Image Perceptions in International Retailing: an Empirical Comparison of IKEA’s

It has been found that customers in the home market perceive the retailer’s store image more positively than customers in the host market, even though this study has found that the retailer’s store image is generally perceived very positively by customers in both examined markets. In addition, this thesis has found that there is a significant difference in how IKEA’s management and customers perceive its store image. It can also be concluded that IKEA management perceived store image more positively than customers. This thesis suggests several practical implications for international retailers, in addition to supporting the bulk of existing theory in this area as well as somewhat filling identified research gaps not sufficiently addressed by previous research

Postkomunističke novotvorine u istočnoj Evropi

Prijevod: Ivan Glaser

Interaction of some polyoxotunstates with acetylcholinesterase

Polyoxometalates (POMs) are polyanionic oligomeric aggregates of transition metal ions, such as tungsten, molybdenum, vanadium, etc. held together by oxygen bridges, with a high density of negative charge. They are relatively stable, some even highly stable in aqueous solutions at biological pH values. In addition to applications in catalysis, separations, analysis, and as electrondense imaging agents, some of these complexes have been shown to exhibit biological activity in vitro as well as in vivo ranging from anti-cancer, antibiotic, and antiviral to antidiabetic effects. Recent investigations reported some polyoxotungstates as reversible inhibitors of acetylcholinesterase (AChE), making them potential anti-Alzheimer’s drugs. AChE is a serine hydrolase mainly found at neuromuscular junctions and cholinergic brain synapses. Its principal biological role is the termination of impulse transmission at cholinergic synapses. Reversible inhibitors of AChE mostly have therapeutic applications, while toxic effects are associated with irreversible AChE activity modulators. Reversible inhibitors play an important role in the pharmacological manipulation of the enzyme activity, and have been applied in the diagnostic and/or treatment of various diseases such as: myasthenia gravis, AD, postoperative ileus, bladder distention, glaucoma, as well as antidote to anticholinergic overdose. The effect of four new synthesized polyoxotungstates soluble in water on AChE activity was studied. AChE is purified from electric eel and commercially available. The enzyme was treated in vitro with polyoxotungstates in the concentration range from 1 × 10-7 to 1 × 10-3 mol/L at 37ºC for 15 minutes, and the incubation time was 12 min. The obtained dependence remaining enzyme activity vs. the inhibitor concentration fitted the sigmoidal function. IC50values, indicating the enzyme sensitivity toward the inhibitor and the inhibitory capacity of the analyzed compounds, were determined from the inhibition sigmoidal curves. Na10[H2W12O42] × 27H2O did not markedly reduce AChE activity at the highest investigated concentration (1 mmol/L). K7[SiV3W9O40] × 10H2O exhibited a weak inhibitory potential, causing 50% decrease in the enzyme activity at 5 × 10-4 mol/L. However, AChE sensitivity in the presence of K7[Ti2PW10O40] was several hundred times higher, reaching IC50 at 1.15 × 10-6 mol/L. Furthermore, (NH4)14[NaP5W30O110] × 31H2O demonstrated the strongest capacity to inhibit AChE. In the presence of its low concentration of 2 × 10-8 mol/L, the enzyme activity was noticeably reduced related to the control value (obtained without inhibitor), while 50% decrease in AChE activity was achieved at 3.8 × 10-7 mol/L.Fourth International Conferenceon Radiation and Applications in Various Fields of Research, RAD 2016, May 23-27, 2016, Niš, Serbi