Didàctica de l’Educació Física

Departament d'Educació. Codi d’assignatura: MP101

Resolución alternativa de conflictos

El presente trabajo hace referencia a cuatro maneras de resolver conflictos, encaminadas a intentar que las partes no lleguen a la vía judicial, por lo tanto evitar así los gastos que se generan por esa vía y la pérdida de tiempo e intentar llegar a un entendimiento entre las partes. Los diferentes modelos que se relacionan, Mediación, Arbitraje, Conciliación y Transacción, tienen como objetivo común la resolución del conflicto, pero la búsqueda y desarrollo de tal fin se realiza de forma diferente.La resolución alternativa de conflictos son métodos de solución de problemas que surgen entre partes enfrentadas. Con ellos se pretende dar solución a los mismos sin llegar a la vía judicial. Para ello terceras personas ajenas a las partes enfrentadas ayudarán a aproximar posturas que propicien una solución pactada y aceptada por los enfrentados y así no llegar a la vía judicial, o cerrar ésta si ya ha comenzado.Grado en Criminologí

Programa de concientización para la conservación y puesta en valor del patrimonio histórico inmueble en el distrito de Lambayeque

El presente trabajo de investigación tiene como objetivo proponer un programa de concientización a los propietarios, autoridades responsables y personas interesadas sobre la importancia de la conservación y puesta en valor del patrimonio histórico inmueble de propiedad privada del Distrito de Lambayeque, fomentando las capacidades, conocimientos, actitudes y valores orientados al cumplimiento de los objetivos estableciendo una cultura basada en la pertenencia a la historia y a la tradición de la región. Este estudio es un tipo de investigación no experimental - transversal, realizado en una población de 40 propietarios de edificaciones declaradas patrimonio histórico inmueble de propiedad privada, teniendo a la concientización de los propietarios de los patrimonios históricos inmuebles y autoridades responsables como variable independiente, a la conservación y puesta en valor del patrimonio histórico inmueble como variables dependientes. Los datos de las variables de concientización a los propietarios y conservación del patrimonio en sus diferentes dimensiones se han obtenido a través de la técnica de la encuesta, de modo que, según los resultados obtenidos podemos decir que los propietarios de viviendas patrimoniales consultados reconocen y valoran su importancia, sin embargo, el factor económico limita su accionar, indicando que cuentan con pocos recursos el 45.9%, y el 16.2% tienen apuros económicos. los propietarios consultados, el 64.9% indicaron que nunca han contado con el apoyo técnico del ministerio de cultura, la presente investigación plantea la puesta en marcha de estrategias de gestión efectivas para aumentar la colaboración y difusión en un corto plazo, siendo necesario articular los lineamientos estratégicos con un plan de concientización siguiendo las siguientes actividades como son: diseñar la ruta de acción del plan de concientización, determinar acciones para la estrategia de sensibilización, identificar acciones para el plan de capacitación / educación, determinar las habilidades midiendo el grado de conocimiento adquirido, estipular acciones para los talleres de comunicación efectiva, fijar acciones para los programas de rescate del patrimonio inmueble, señalar acciones para el apoyo a los propietarios de inmuebles patrimoniales

A role for antizyme inhibitor 2 in the biosynthesis and content of serotonin and histamine in mouse mast cells

Polyamines (putrescine, spermidine and spermine; PAs) are essential for the majority of living cells. Antizymes and antizyme inhibitors are key regulatory proteins of PA levels by affecting ornithine decarboxylase and PA uptake. In addition to PAs, mast cells (MC) synthesize and store in their granules histamine (Hia) and serotonin (5-HT), which are critical for their function. Our previous studies have indicated a metabolic interplay among PAs, Hia and 5-HT in this cell type. For instance, we showed that PAs affect Hia synthesis during early stages of IL-3-induced bone marrow cell differentiation into bone marrow derived MCs (BMMCs) and demonstrated that PAs are present in MC secretory granules and are important for granule homeostasis, including Hia storage and 5-HT levels. A few years ago, a novel antizyme inhibitor (AZIN2) was described whose expression is restricted to a few tissues and cell types including brain, testis and MCs. In MCs, it was recently proposed that AZIN2 could act as a local regulator of PA biosynthesis in association with 5-HT-containing granules and with 5-HT release following MC activation. To gain insight into the role of AZIN2 in the biosynthesis and storage of 5-HT and also Hia, we have generated BMMCs from both wild-type and transgenic mice with severe Azin2 hypomorphism, and have analyzed the content of PAs, 5-HT and Hia, and some elements of their metabolisms. Spermine and 5-HT levels were reduced in Azin2 hypomorphic BMMCs compared with wild-type controls, whereas the amount of Hia was increased. Accordingly, the level of tryptophan hydroxylase 1 (the key enzyme for 5-HT biosynthesis) was reduced and the amount of enzymatic activity of histidine decarboxylase (the enzyme responsible for Hia biosynthesis) was increased in Azin2 hypomorphic BMMCs. Taken together, our results show evidence that AZIN2 has an important role in the regulation of 5-HT and Hia biosynthesis and storage in MCsUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This work was supported by SAF2011-26518 (MINECO, Spain) and P10-CVI-6585 and Bio-267 (Junta de Andalucia, Spain). CIBERER is an iniciative of Instituto de Salud Carlos III (Spain)

A role for antizyme inhibitor 2 in the biosynthesis and content of histamine and serotonin in mouse mast cells

Polyamines (putrescine, spermidine and spermine; PAs) are required for the survival of the majority of living cells. Antizymes and antizyme inhibitors are key regulatory proteins of PA levels by affecting ornithine decarboxylase, the rate-limiting biosynthetic enzyme, and PA uptake. In addition to PA, mast cells (MC) synthesize and store in their granules the biogenically active amines histamine (Hia) and serotonin (5-HT), which are of critical importance for their function. Previously, we have performed several studies in this cell type regarding the interplay between the metabolisms of PAs and Hia and 5-HT. Our results showed that PAs affect Hia synthesis during early stages of IL-3-induced bone marrow cell differentiation into bone marrow derived MCs (BMMCs) and demonstrated that PAs are present in MC secretory granules and are important for granule homeostasis, including Hia storage and 5-HT levels. A few years ago, a novel antizyme inhibitor (AZIN2) was described. In contrast to AZIN1, AZIN2 expression is restricted to a few tissues and cell types including brain, testis and MCs. In MCs, it was recently described that AZIN2 could act as a local regulator of PA biosynthesis in association with the 5-HT granule content and release. At present, our aim is to gain further insight into the role of AZIN2 in the biosynthesis, storage and release of both Hia and 5-HT. In this study, we have generated BMMCs from both wild-type and transgenic mice with severe Azin2 hypomorphism, and have analyzed the content of PAs, Hia and 5-HT, and some elements of their metabolisms. Both PAs and 5-HT levels were reduced in Azin2 hypomorphic BMMCs compared with wild-type controls, whereas the amount of Hia was increased. Accordingly, the level of tryptophan hydroxylase 1 (the key enzyme for 5-HT biosynthesis) was reduced and the amount of enzymatic activity of histidine decarboxylase (the enzyme responsible for histamine biosynthesis) was increased in Azin2 hypomorphic BMMCs. Taken together, our results show evidence that AZIN2 has an important role in the regulation of Hia and 5-HT biosynthesis and storage in MCs. Department of Molecular Biology and Biochemistry, and CIBER de Enfermedades Raras (CIBER-ER), Faculty of Sciences, University of Málaga, Málaga 29071, Spain. Corresponding author: I. Fajardo ([email protected]) This work was supported by SAF2011-26518 (MINECO, Spain) and P10-CVI-6585 and Bio-267 (Junta de Andalucia, Spain). CIBERER is an iniciative of Instituto de Salud Carlos III (Spain).Universidad de Málaga. Campus de Excelencia Internacional Andalucía-Tech. SAF2011-26518 (MINECO, Spain) and P10-CVI-6585 and Bio-267 (Junta de Andalucia, Spain. CIBERER is an iniciative of Instituto de Salud Carlos III (Spain)

Structural and functional interaction between polyamine related molecules and biological membranes

La comunicación describe el conocimiento actual sobre las interacciones de biomoléculas relacionadas con el metabolismo de poliaminas con las estructuras y funciones de las membranas biológicasChanges induced by PA on nucleic acid (NA) conformation and synthesis is proven to be a major reason for PA essentiality (1-3). However, PA interactions with other polyanions, for instance polyanionic membrane lipid bilayers and glyosaminoglycans have received less attention (3-4). The functional importance of these interactions still is an obscure but interesting area of cell and molecular biology, especially in mammalian cells for which specific PA transport systems are not fully characterized (5). In mammals, activity and turnover of the polyamine (PA) synthesis key enzyme is controlled by a set of proteins: Antizymes (OAZ1-3) and antizyme inhibitors (AZIN1 and 2). It is demonstrated that AOZ modulate polyamine uptake (6), and that PA transport to mitochondria is linked to the respiratory chain state and modulates mitochondrial permeability transition (7). Antizyme expression variants have been located in mitochondria, being proposed as a proapoptotic factor (7-8). AZIN 2 is only expressed in a reduced set of tissues that includes mast cells, where it is associated to mast cell granules membrane (9). This fact, together to the abnormalities observed in bone marrow derived mast cell granules when they are differentiated under restricted PA synthesis conditions (10 and unpublished results), point out to important roles of PA and their related proteins in structure and function of mast cell granules. We will also present novel biophysical results on tripartite interactions of PA that remark the interest of the characterization of PA interactions with lipid bilayers for biomedicine and biotechnology. Thus, the information reported in this paper integrates previously reported information with our still unpublished results, all indicating that PA and their related proteins also are important factors for structure and dynamics of biological membranes and their associated functions essential in human physiology; for instance, solute interchange with the environment (uptake and secretion), oxidative metabolism and apoptosis. The importance of these involved processes for human homeostasis claim for further research efforts. 1. Ruiz-Chica J, Medina MA, Sánchez-Jiménez F and Ramírez FJ (2001) Fourier Transform Raman study of the structural specificities on the interaction between DNA and biogenic polyamines. Biophysical J. 80:443-454. 2. Lightfoot HL, Hall J (2014) Endogenous polyamine function--the RNA perspective. Nucleic Acids Res. 42:11275-11290. 3. Igarashi K, Kashiwagi K (2010) Modulation of cellular function by polyamines. Int J Biochem Cell Biol. 42:39-51. 4. Finger S, Schwieger C, Arouri A, Kerth A, Blume A (2014) Interaction of linear polyamines with negatively charged phospholipids: the effect of polyamine charge distance. Biol Chem. 395:769-778. 5. Poulin R, Casero RA, Soulet D. (2012) Recent advances in the molecular biology of metazoan polyamine transport. Amino Acids. 42:711-723. 6. Kahana C (2009) Regulation of cellular polyamine levels and cellular proliferation by antizyme and antizyme inhibitor. Essays Biochem. 4:47-61. 7. Agostinelli E, Marques MP, Calheiros R, Gil FP, Tempera G, Viceconte N, Battaglia V, Grancara S, Toninello A (2010) Polyamines: fundamental characters in chemistry and biology. Amino Acids 38:393-403. 8. Liu GY, Liao YF, Hsu PC, Chang WH, Hsieh MC, Lin CY, Hour TC, Kao MC, Tsay GJ, Hung HC (2006) Antizyme, a natural ornithine decarboxylase inhibitor, induces apoptosis of haematopoietic cells through mitochondrial membrane depolarization and caspases' cascade. Apoptosis 11:1773-1788. 9. Kanerva K, Lappalainen J, Mäkitie LT, Virolainen S, Kovanen PT, Andersson LC (2009). Expression of antizyme inhibitor 2 in mast cells and role of polyamines as selective regulators of serotonin secretion. PLoS One 31:e6858. 10. García-Faroldi G, Rodríguez CE, Urdiales JL, Pérez-Pomares JM, Dávila JC, Pejler G, Sánchez-Jiménez F, Fajardo I (2010) Polyamines are present in mast cell secretory granules and are important for granule homeostasis. PLoS One 30:e15071.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Segumiento de la inundación en la marisma de Doñana: resultados del ciclo 2009/2010 y efecto del dique de la montaña del río en el proceso de inundación

En el Espacio Natural de Doñana, dentro del marco del Plan de Aguas, se vienen realizando desde el año 1994 el seguimiento de la inundación de la marisma, de la meteorología, del control de la calidad de las aguas superficiales y los cauces vertientes a la marisma y el seguimiento de las actuaciones del Proyecto de Regeneración Hídrica Doñana 2005, del que aún quedan por realizar algunas actuaciones. Las condiciones meteorológicas del invierno de 2009/2010, marcadas por unas precipitaciones muy por encima de la media, han permitido observar por primera vez los efectos de algunas de las actuaciones del Proyecto Doñana 2005, especialmente la influencia de un dique conocido como la Montaña del Río, que aísla la marisma del estuario del Guadalquivir y que ha generado una sobre-inundación de las zonas más elevadas de la marisma, provocando la muerte de muchas especies que no están adaptadas a condiciones de inundación tan intensas como las que se han dado este año. La eliminación de este dique es una de las actuaciones que faltan por ejecutar del Proyecto Doñana 2005. El seguimiento de la inundación en la marisma está basado en una red de escalas limnimétricas, un Modelo Digital del Terreno, imágenes satélite y un Modelo Matemático.Since 1994 a significant part of wetland management at DNS is the monitoring programme, that mainly concerns water levels, water quality and meteorology, as it was stablished in the framework of the Water Managment Plan. The2009-2010 winter weather conditions marked by well above average rainfall, have allowed to observe for the first time some effects of the Doñana 2005 Project, especially the influence of a dam known as the River Mountain which isolates the marshes of the Gudalquivir estuary and has generated an over flooding of the higher areas of the marsh, killing many species that are not adapted to intense flooding conditions as those that have happened this year. The removal of this dam is one of the actions remaining to be done to complete the Doñana 2005 Project.The flood monitoring in Doñana Natural Space Marshes is based in a water measuring poles network, in a Digital terrain Model generated by LIDAR, Satellite Images and a Mathematical Flooding Model

Association between Health-Related Physical Fitness and Self-Rated Risk of Depression in Adolescents: Dados Study

Depression is the most common mental disorder, affecting around 5% of adolescents. Physical fitness is considered a powerful marker of physical and mental health. The scientific results on the relationship between physical fitness and depression in the adolescent population are mixed. Therefore, the aim of the current study was to analyse the association between objectively assessed physical fitness and self-rated risk of depression in a group of adolescents. A total of 225 participants (44% girls), aged 13.9 ± 0.3 years, from the Deporte, ADOlescencia y Salud (DADOS) study were included in the analyses. Field-based Assessing Levels of Physical fitness and Health in Adolescents (ALPHA) health-related fitness test battery was used to objectively assess physical fitness components. The Behavior Assessment System for Children (BASC) level 3 was used to evaluate self-rated risk of depression. Our results showed that self-rated risk of depression was inversely associated with cardiorespiratory fitness (β = −0.172), as well as positively associated with body mass index (β = 0.146) and waist circumference (β = 0.137) (all p < 0.05). Adolescents with low levels of cardiorespiratory fitness had significantly higher odds of self-rated risk of depression (OR = 7.17; 95% CI, 1.51–33.95). These findings suggest that health-related physical fitness, particularly cardiorespiratory fitness and body composition, is associated with depression in adolescents

Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides

International audienceThis is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules

Exercise addiction risk and health in male and female amateur endurance cyclists

To determine the relationship between the risk of exercise addiction (REA) and health status in amateur endurance cyclists. Methods In 859 (751 men and 108 women) cyclists and 718 inactive subjects (307 men and 411 women), we examined the REA (Exercise Addiction Inventory), training status (volume, frequency, experience, and performance), socioeconomic status, quality of life (QoL) (SF-12), quality of sleep (Pittsburgh Sleep Quality Index), anxiety and depression (Hospital Anxiety and Depression Scale), and cardiometabolic risk: body mass index, physical activity (International Physical Activity Questionnaire), physical condition (International Fitness Scale), adherence to the Mediterranean diet (Mediterranean Diet Adherence Screener), alcohol and tobacco consumption. Results In total, 17% of the cyclists showed evidence of REA and 83% showed low REA. REA occurred independent of age, sex, training, and socioeconomic status (all ps > .05). Regardless of REA, the cyclists displayed a better physical QoL and a lower cardiometabolic risk than the inactive subjects (all ps < .05). The cyclists with REA displayed worse values of mental QoL, quality of sleep, and anxiety than cyclists with low REA (all ps < .05). The REA group had better values of mental QoL and anxiety and similar values of quality of sleep than the inactive subjects. The differences in mental QoL between the REA and low REA groups were significantly greater in women (p = .013). There was no Addiction × Sex interaction in the other analyzed variables. Conclusion Our results suggest that an increased prevalence of REA limits the benefits that amateur endurance cycling has on mental health and quality of sleep