Metabolic Syndrome as a Cardiovascular Disease Risk Factor: Patients Evaluated in Primary Care

To estimate the prevalence of metabolic syndrome (MS) in a population receiving attention in primary care centers (PCC) we selected a random cohort of ostensibly normal subjects from the registers of 5 basic-health area (BHA) PCC. Diagnosis of MS was with the WHO, NCEP and IDF criteria. Variables recorded were: socio-demographic data, CVD risk factors including lipids, obesity, diabetes, blood pressure and smoking habit and a glucose tolerance test outcome. Of the 720 individuals selected (age 60.3 ± 11.5 years), 431 were female, 352 hypertensive, 142 diabetic, 233 pre-diabetic, 285 obese, 209 dyslipemic and 106 smokers. CVD risk according to the Framingham and REGICOR calculation was 13.8 ± 10% and 8.8 ± 9.8%, respectively. Using the WHO, NCEP and IDF criteria, MS was diagnosed in 166, 210 and 252 subjects, respectively and the relative risk of CVD complications in MS subjects was 2.56. Logistic regression analysis indicated that the MS components (WHO set), the MS components (IDF set) and the female gender had an increased odds ratio for CVD of 3.48 (95CI%: 2.26–5.37), 2.28 (95%CI: 1.84–4.90) and 2.26 (95%CI: 1.48–3.47), respectively. We conclude that MS and concomitant CVD risk is high in ostensibly normal population attending primary care clinics, and this would necessarily impinge on resource allocation in primary care

Biases in abundance derivations for metal-rich nebulae

Using ab-initio photoionization models of giant HII regions, we test methods for abundance determinations based on a direct measurement of the electron temperature, now possible even for moderate to high-metallicity objects. We find that, for metallicities larger than solar, the computed abundances deviate systematically from the real ones, generally by larger amounts for more metal-rich HII regions. We discuss the reasons for this, and present diagrams allowing the reader to better understand the various factors coming into play. We briefly discuss less classical methods to derive abundances in metal-rich HII regions. In particular, we comment on the interest of the oxygen and carbon recombination lines. We also show that, contrary to the case of giant HII regions, the physical conditions in bright extragalactic planetary nebulae are such that their chemical composition can be accurately derived even at high metallicities. Thus, extragalactic planetary nebulae are promising potential probes of the metallicity of the interstellar medium in the internal parts of spiral galaxies as well as in metal-rich elliptical galaxies.Comment: 15 pages, 13 figures, Accepted for publication in A&

A modern guide to quantitative spectroscopy of massive OB stars

Quantitative spectroscopy is a powerful technique from which we can extract information about the physical properties and surface chemical composition of stars. In this chapter, I guide the reader through the main ideas required to get initiated in the learning process to become an expert in the application of state-of-the-art quantitative spectroscopic techniques to the study of massive OB stars. NB: This chapter is intended to serve to young students as a first approach to a field which has attracted my attention during the last 20 years. I should note that, despite its importance, at present, the number of real experts in the field around the world is limited to less than 50 people, and about one third of them are close to retirement. Hence, I consider that this is a good moment to write a summary text on the subject to serve as guideline for the next generations of students interested in joining the massive star crew. If you are one of them, please, use this chapter as a first working notebook. Do not stop here. Dig also, for further details, into the literature I quote along the text. And, once there, dig even deeper to find all the original sources explaining in more detail the physical and technical concepts that are presently incorporated into our modern (almost) automatized tools.Comment: Accepted for publication in the book "Reviews in Frontiers of Modern Astrophysics: From Space Debris to Cosmology" (eds Kabath, Jones and Skarka; publisher Springer Nature) funded by the European Union Erasmus+ Strategic Partnership grant "Per Aspera Ad Astra Simul" 2017-1-CZ01-KA203-03556

MUSE 3D spectroscopy of BA-type supergiants in NGC 300

We present the results obtained using spectroscopic data taken with the intermediateresolution Multi Unit Spectroscopic Explorer (MUSE) of B and A-type supergiants and bright giants in the Sculptor Group galaxy NGC 300. For our analysis, a hybrid local thermodynamic equilibrium (LTE) line-blanketing+non-LTE method was used to improve the previously published results for the same data. In addition, we present some further applications of this work, which includes extending the flux-weighted gravity luminosity relationship (FGLR), a distance determination method for supergiants. This pioneering work opens up a new window to explore this relation, and also demonstrates the enorm

MUSE crowded field 3D spectroscopy in NGC 300 II. Quantitative spectroscopy of BA-type supergiants

Aims. A quantitative spectral analysis of BA-type supergiants and bright giants in an inner spiral arm region of the nearby spiral galaxy NGC 300 is presented, based on observations with the Multi Unit Spectroscopic Explorer (MUSE) on the European Southern Obsevatory, Very Large Telescope. The flux-weighted gravity–luminosity relationship (FGLR), a stellar spectroscopic distance determination method for galaxies, is extended towards stars at lower luminosities. Methods. Point spread function fitting 3D spectroscopy was performed with PampelMUSE on the datacube. The 16 stars with the highest signal-to-noise ratios are classified with regard to their spectral type and luminosity class using Galactic templates. They were analysed using hybrid non-local thermodynamic equilibrium model spectra to fit the strongest observed hydrogen, helium, and metal lines in the intermediate-resolution spectra. Supplemented by photometric data, this facilitates fundamental stellar parameters and interstellar reddening which have yet to be determined. Results. Effective temperatures, surface gravities, reddening E(B−V), bolometric magnitudes and luminosities, as well as radii and masses are presented for the sample stars. The majority of the objects follow the FGLR as established from more luminous BA-type supergiants in NGC 300. An increase in the scatter in the flux-weighted gravity–luminosity plane is observed at these lower luminosities, which is in line with predictions from population synthesis models

Probing nucleation, reverse annealing, and chaperone function along the reaction path of HIV-1 single-strand transfer

Reverse transcription of the HIV-1 genome involves several nucleic acid rearrangement steps that are catalyzed (chaperoned) by the nucleocapsid protein (NC), including the annealing of the transactivation response region (TAR) RNA of the genome to the complementary sequence (TAR DNA) in minus-strand strong-stop DNA. It has been extremely challenging to obtain unambiguous mechanistic details on the annealing process at the molecular level because of the kinetic involvement of a complex and heterogeneous set of nucleic acid/protein complexes of variable structure and variable composition. Here, we investigate the in vitro annealing mechanism using a multistep single-molecule spectroscopy kinetic method. In this approach, an immobilized hairpin is exposed to a multistep programmed concentration sequence of NC, model complementary targeted-oligonucleotides, and buffer-only solutions. The sequence controllably “drags” single immobilized TAR hairpins among the kinetic stable states of the reaction mechanism; i.e., reactants, intermediates, and products. This single-molecule spectroscopy method directly probes kinetic reversibility and the chaperone (catalytic) role of NC at various stages along the reaction sequence, giving access to previously inaccessible kinetic processes and rate constants. By employing target oligonucleotides for specific TAR regions, we kinetically trap and investigate structural models for putative nucleation complexes for the annealing process. The new results lead to a more complete and detailed understanding of the ability of NC to promote nucleic acid/nucleic acid rearrangement processes. This includes information on the ability of NC to chaperone “reverse annealing” in single-strand transfer and the first observation of partially annealed, conformational substates in the annealing mechanism