Running Big Data Privacy Preservation in the Hybrid Cloud Platform

Now a day’s cloud computing has been used all over the industry, due to rapid growth in information technology and mobile device technology. It is more important task, user’s data privacy preservation in the cloud environment. Big data platform is collection of sensitive and non-sensitive data. To provide solution of big data security in the cloud environment, organization comes with hybrid cloud approach. There are many small scale industries arising and making business with other organization. Any organization data owner or customers never want to scan or expose their private data by the cloud service provider. To improve security performance, cloud uses data encryption technique on original data in public cloud. Proposed system work is carried out how to improve image data privacy preserving in hybrid cloud. For that we are implementing image encryption algorithm based on Rubik’s cube principle improves the image cryptography for the public cloud data securit

Evaluation of Diuretic Activity of Aqueous and Methanol Extracts of Lepidium sativum Garden Cress (Cruciferae) in Rats

Purpose: The present study was undertaken to investigate diuretic effect of aqueous and methanol extracts of the dried seeds of Lepidium sativum in normal rats.Method: Aqueous and methanol extracts of L. sativum seeds were administered to experimental rats orally at doses of 50 and 100 mg/kg p.o. Hydrochlorothiazide (10 mg/kg) was used as positive control instudy. The diuretic effect of the extracts was evaluated by measuring urine volume, sodium and potassium content, conductivity and pH.Result: Urine volume was significantly increased by the two doses of aqueous and methanol extracts in comparison to control group. While the excretion of sodium was also increased by both extracts, potassium excretion was only increased by the aqueous extract at a dose of 100 mg/kg. There was no significant change in the conductivity and pH of urine after administration of the L. sativum extracts. The diuretic effect of the extracts was comparable to that of the reference standard (hydrochlorothiazide) and the methanol had the additional advantage of a potassium-conserving effect.Conclusion: We can conclude that aqueous and methanol extracts of L. sativum produced notable diuretic effect which appeared to be comparable to that produced by the reference diuretic HCTZ. The present study provides a quantitative basis for explaining the folkloric use of L. sativum as a diuretic agent in Moroccan population

Evaluation of Pharmacodynamic interaction between Tinospora Cordifolia Alcoholic extract and Gliclazide : An herb-drug interaction study

Many diabetic people today consume herbs or herbal formulations along with prescription and non-prescription medications which may result in the herb-drug interaction Tinospora Cordifolia, with berberine being one of the most abundant active phytoconstituent widely used as an antidiabetic While Gliclazide is indicated to treat type 2 diabetes mellitus whichacts asan insulin secretagogue .T. Cordifolia is a potent inhibitor of CYP2C9 and Gliclazide is known to be metabolized by this enzyme. Potential Pharmacodynamic herb drug interaction might be possible in case of co administration of both. The pharmacodynamic interaction between TCE and Gliclazide was evaluated on hypoglycemic activity in normal and streptozotocinnicotinamide-induced diabetic rats. The study was conducted in 2 parts viz. acute study and sub-acute study in both normal and diabetic animals. The serum triglyceride level and histopathology of pancreawas performed to assess effect on glucose metabolism and pancreas.FTIR Analysis was also carried out to evaluate the interaction between functional groups. The combination showed pharmacodynamic interaction as reduction the time of onset of action and increasing the duration of action of gliclazide when administered in combination with T. cardiofolia. In FTIR studies of combination showed no physical interaction betweenfunctional groups suggesting both drugs might be acting on the different receptors. The study concludes that the combination of Gliclazide with TCE showed an increase in the hypoglycemic effect as compared to the gliclazide alone in STZ-NIC induced diabetic rats. This might be utilized clinically as a beneficial drug interaction in patients after thorough investigations in clinical studie

Antidiabetic activity of Plumeria rubra L. in normal and alloxan induced diabetic mice

Background: Diabetes mellitus is a major source of morbidity in developed countries. In spite of the introduction of hypoglycemic agents, diabetes and related complications continue to be a major medical problem. Our present study aims to investigate the antidiabetic activity of aqueous extract of plumeria rubra (PR) in experimental animals.Methods: PR extract was subjected to antidiabetic study in alloxan induced diabetic model at three-dose levels 100,200 and 400 mg/kg respectively. It was also tested for hypoglycemic activity at same dose levels. Diabetes was induced by alloxan monohydrate (150 mg/kg, i.p.). PR extracts and standard drug glibenclamide (10 mg/kg, p.o.) was administered to animals for 28 days. The blood glucose, serum cholesterol, serum triglyceride, high density lipoprotein, low density lipoprotein, glycosylated hemoglobin. Body weights were assessed in the experimental animals. Histopathological observations during 28 days treatment were also evaluated.Results: PR extract induced significant reduction (P<0.001) in fasting blood glucose levels in normal and alloxan-induced diabetic mice. Significant differences were observed in serum lipid profiles, glycosylated haemoglobin by extract treated-diabetic animals, when compared with the diabetic control and normal animals. The protective effect of PR was also evident from the histopathological examination on pancreas, liver and kidney. It reduced the injuries induced by alloxan.Conclusions: PR exhibited significant antihyperglycemic activity in normal and alloxan-induced diabetic mice. The results of the present study provide support to the traditional usage of the plant in diabetes

Antinociceptive and Anti-Inflammatory Effects of Solvent Extracts of Tagetes erectus Linn (Asteraceae

Purpose: Traditionally, the leaves of Tagetes erectus L. are used in India for the alleviation of pain and inflammation. The objective of this study was to investigate the antinociceptive and anti-inflammatoryactivities of this plant material in an animal model.Methods: The chloroform, methanol and ether extracts of the leaves of Tagetes erectus L. (family: Asteraceae) were tested against acetic acid-induced writhing in mice and carrageenan-induced pawoedema in rats in order to assess their antinoceciptive and anti-inflammatory activities, respectively. The doses administered intraperitoneally (I.P.) ranged from 100 to 400 mg/kg body weight, andacetylsalicylic acid (ASA) and phenylbutazone were the reference standards for the antinoceciptive and anti-inflammatory tests, respectively.Results: The extracts showed antinociceptive and anti-inflammatory properties at doses between 200-400 mg/kg. They inhibited significantly (P < 0.005), in a dose-dependant manner, induced writhingreflexes in mice. The antinoceciptive effect was comparable to that of ASA which served as the reference standard. Similarly, the extracts significantly (

Quantitative Determination of Norepinephrine by HPLC in Rodent Urine Sample

Abstract Norepinephrine, a key neurotransmitter that has been linked to a variety of neuropsychiatric diseases. However, there is limited work on employing HPLC-UV to estimate monoamines in biological samples using HPLC-UV method. The present study explores the detection of norepinephrine in rat urine sample, developing a easy, precise validated HPLC method. The o-phosphoric acid (80%):acetonitrile (70:30) combination was used as the mobile phase, and a flow rate of 0.5 ml/min was used to produce the chromatographic separation on a C8 column (250 x 4.6 mm, 5 m). The detection was observed at λmax 275 nm with better sensitivity. According to the parameters indicated in the ICH guidelines (Q2A; Q2B), the procedure was verified. The linearity range was selected from 10-35 µg/mL, r2 =0.966, LOD (1.17 µg/mL), LOQ (3.55 µg/mL), ret. time (4 min). The technique has demonstrated that it is repeatable and recoverable within the given range. Thus can be used for routine analysis of norepinephrine in urine samples

Preliminary Pharmacognostic, Physicochemical and Phytochemical Evaluation of Plumeria Obtuse Seed Pods

Plumeria obtuse L. (Apocynaceae) is an ornate outdoor plant. The plant was traditionally used during accidentalinjuries. However, the pharmacognosy of this plant is very poorly explored. Therefore, we have conducted this study to assess the distinctive qualities of the P. obtusa. To investigate P. obtusa seed pods’ preliminary pharmacognostic, physical-chemical, phytochemical, microscopic, and phytoconstituent potential. Initially, the shape and microscopic characteristics of plant seed pods were assessed. Physicochemical analysis was used for the standardization. Utilizing several chemical techniques, phytoconstituents were evaluated qualitatively. This was followed by quantitative estimation and analytical profiling of various phytoconstituents. The basic characteristics of the seed pod have been documented by macroscopy to be its brown color, sweet aroma, bitter flavor, coarse texture, and rough fracture. Microscopy showed the existence of vascular bundles, lignified fibers, calcium oxalate crystals and arteries. The results of the physicochemical analysis revealed no foreign organic matter, 2.8 % weight-average moisture content and a high total ash value of 14.80 compared to an acid insoluble ash value of 0.70, which indicated that there was less inorganic matter in the plant. The extractive values were 3.93, 6.03 and 10.16 % w/w for water soluble, alcohol soluble and hydro-alcoholic soluble extracts respectively. Flavonoids, glycosides, saponins, phenolic constituents, tannins and carbohydrates were found during early phytochemical analysis. Instrumental analysis has given an idea about functional groups present whereas GCMS technique helped in identification of phytoconstituents. The results of this study can be significantly used as a reference support for quality control and standardization of P. obtusa and preparation of a monograph of plant

Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency

Mounting evidence indicated that human mesenchymal stem cells (hMSCs) are responsive not only to biochemical but also to physical cues, such as substrate topography and stiffness. To simulate the dynamic structures of extracellular environments of the marrow in vivo, we designed a novel surrogate substrate for marrow derived hMSCs based on physically cross-linked hydrogels whose elasticity can be adopted dynamically by chemical stimuli. Under frequent mechanical stress, hMSCs grown on our hydrogel substrates maintain the expression of STRO-1 over 20 d, irrespective of the substrate elasticity. On exposure to the corresponding induction media, these cultured hMSCs can undergo adipogenesis and osteogenesis without requiring cell transfer onto other substrates. Moreover, we demonstrated that our surrogate substrate suppresses the proliferation of hMSCs by up to 90% without any loss of multiple lineage potential by changing the substrate elasticity every 2nd days. Such “dynamic in vitro niche” can be used not only for a better understanding of the role of dynamic mechanical stresses on the fate of hMSCs but also for the synchronized differentiation of adult stem cells to a specific lineage