The Effect of Growth Regulator Substance on Shoot Cutting of Jamblang (Syzygium cumini (L.) Skeels

Development of jamblang (Syzygium cumini) as medicinal plant currently constrained by the aspects of cultivation technology (vegetative propagation), so the provision of medicinal plants for the mass scale was very difficult. The objective of this research was to evaluate the effect of five doses of  growth regulator substance on growth of S. cumini’s shoot cuttings. The active ingredient composition of hormone/ Growth Regulator Substance (GRS) in this study were Naphthalene Acetic Acid (NAA) 3 percent and Naphthalene Acetamide (NAAm) 0.75percent. The experiment was arranged in Completely Random Design using 5 concentrations of growth regulator substance namely 0g.10ml-1(control), 2g.10 ml-1, 4g.10ml-1, 10g.10ml-1 and powder (not dissolved). The results of this experiment proved that  4g.10 ml-1of growth regulator substance produce the highest height growth (29.84 cm or 21.74 percent increment), the highest number of leaves (23.72 pieces or 4.63 percent increment), the heaviest dry weight of stem and leaves (3.36 gram or 43.59 percent increment) and the highest top-root ratio (6.55 or 10.08 percent increment). The highest survival percentage was resulted from GRS powder treatment (80 percent or 18.81 percent increment), but did not significantly different to GRS treatment of 4g.10 ml-1 (77.33 percent). The application of 4g.10 ml-1 is recommended to use to produce the better growth of cuttings planted on soil

FEASIBILITY STUDY OF BUSINESS IN AGARWOOD INOCULATION AT DIFFERENT STEM DIAMETERS AND INOCULATION PERIODS

Indonesia signifies as the biggest agarwood producer country in the world. Its demand and price tend to increase and have brought about over exploitation of agarwood. Consequently, its population in nature has decreased significantly. To overcome the situation, since 1995, agarwood has been included in the CITES Appendix II. However, illegal exploitation remains persistent and reaches an excessive level. In order to deal with it, agarwood cultivation and its artificial production have been undertaken at several provinces in Indonesia. Some supporting factors for agarwood cultivation and artificial production are the availability of potential land for extensive cultivation, appropriate agro climate condition, simple cultivation technique and already being well adopted by farmers, the availability of necessary pathogen for agarwood inoculation, and the increasing demand with relatively high price. The research aims to analyze the feasibility study of agarwood inoculation business at several stem diameters (15 - 25 cm; 26 -35 cm and 36 - 40 cm) and periods of inoculation (1 - 5 years). Data were collected through field observation and literature study. The results showed that inoculation on agarwood producer tree stands at 12.5% interest rate afforded positive net present value (NPV), internal rate of return (IRR) is much higher than market interest and benefit cost (B/C) ratio >2 for those three diameter classes. Furthermore, if agarwood harvesting is delayed until five years after inoculation, NPV, IRR and B/C ratio would be much higher. It can be concluded that inoculation on agarwood producer tree stands (at appropriate age for inoculation) is feasible to be developed

Phosphate Solubilizing and Antifungal Activity of Root Endophyte Isolated from Shorea leprosula Miq. And Shoreal selanica (DC) Blume

Fungal endophytes are fungi that lives within plant tissues without causing apparent disease. It is also suggested that these fungi have ability to enhance plant growth and plant resistancy against pest and disease. This research is a preliminary study about root fungal endophytes in dipterocarp since there are lack research concerning about this study focus. We examined root fungal endophyte  isolated from seedling of Shorea leprosula and Shorea selanica taken from Dramaga Experimental Forest, Bogor.  Furthermore, we also tried to find out the fungal potential ability to solubilize phosphate and suppres fungal pathogen by in vitro assay. Surface sterilization method was used to isolated fungal endophytes from root tissues. Trichoderma spirale, Velsalceae sp., Melanconiela ellisii, Chaetosphaeria callimorpha, and Trichoderma asperellum were isolated during this study. These fungi appear to have specific association between fungal species and host plant, but no evidence of fungal order-level specificiation in S. leprosula and S. selanica. In vitro test also suggested that root fungal endophyte Trichoderma spirale and Melanconiella elisii have potential ability to solubilize inorganic phosphate. In addition, this result also present that root fungal endophyte T. spirale and T. asperellum have the potential to inhibit pathogen fungi Fusarium sp.

Arbuscular Mycorrhizal Fungi Increased Plant Growth and Nutrient Concentrations of Milkwood Tropical Tree Species Alstonia Scholaris Under Greenhouse Conditions

The objective of this study was to determine the effect of five arbuscular mycorrhizal (AM) fungi on the early growth of Alstonia scholaris (milkwood) seedlings. The seedlings were inoculated with Glomus clarum Nicholson & Schenk, Gigaspora decipiens Hall & Abbott, Glomus sp. ACA Tulasne & Tulasne, Entrophospora sp. Ames & Scheneider, and Glomus sp. ZEA Tulasne & Tulasne, and uninoculated (control) under greenhouse conditions. Percentage of AM colonization, plant growth, survival rate, mycorrhizal dependency (MD), shoot nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) concentrations were measured after 150 days. Survival rates were higher in the AM-colonized seedlings at 150 days after transplantation than those in the control seedlings. Mycorrhizal Dependency (MD) values were 80, 78, 79, 78 and 78% in A. scholaris inoculated with G. clarum, G. decipiens, Glomus sp. ACA, Entrophospora sp., and Glomus sp. ZEA, respectively. Shoot N, P, K, Ca and Mg content of the seedlings were increased by AM fungi as much as 82-86, 81-86, 81-86, 88-91 and 85-90%, respectively. The percentage of AM colonization of A. scholaris ranged from 64 to 91 %. Colonization by five AM fungi increased plant height, diameter, total fresh weight, total dry weight and total length root. Glomus clarum was more effective in improving nutrient content and plant growth of A. scholaris than G. decipiens, Entrophospora sp., Glomus sp. ZEA and Glomus sp. ACA. Total root length of A. scholaris ranged from 1,180 to 1,310 cm. The results suggest that AM fungi can accelerate the establishment of the seedling stocks of A. scholaris. This finding would contribute to the effort of establishing A. scholaris plantation

Arbuscular Mycorrhizal Fungi Increased Early Growth of Gaharu Wood of Aquilaria Malaccencsis and a. Crasna Under Greenhouse Conditions

Gaharu wood stand has an important source of profits to the forest community in South and Southeast Asia tropical forest countries, but Aquilaria species have reduced in number and turn out to be endangered due to overexploitation. Today, the planting stocks of Aquilaria species are not sufficient to sustain the yield of gaharu wood and promote forest conservation. The objective of this study was to determine the effect of five arbuscular mycorrhizal (AM) fungi: Entrophospora sp., Gigaspora decipiens, Glomus clarum, Glomus sp. ZEA, and Glomus sp. ACA, on the early growth of Aquilaria malaccensis and A. crasna under greenhouse conditions. The seedlings of Aquilaria spp. were inoculated with Entrophospora sp., Gi. decipiens, Glomus clarum, Glomus sp. ZEA, Glomus sp. ACA and uninoculated (control) under greenhouse conditions. Then, percentage AM colonization, plant growth, survival rate and nitrogen (N) and phosphorus (P) content and mycorrhizal dependence (MD) were measured. The percentage AM colonization of A. malaccensis and A. crasna ranged from 83 to 97% and from 63 to 78%, respectively. Colonization by five AM fungi increased plant height, diameter, and shoot and root dry weights. N and P content of the seedlings were also increased by AM colonization. Survival rates were higher in the AM-colonized seedlings at 180 days after transplantation than those in the control seedlings. The MD of Aquilaria species was higher than 55 %. The results suggested that AM fungi can be inoculated`to Aquilaria species under nursery conditions to obtain vigorous seedlings, and the field experiment is underway to clarify the role of AM fungi under field conditions

Feasibility Study of Business in Agarwood Inoculation at Different Stem Diameters and Inoculation Periods

Indonesia signifies as the biggest agarwood producer country in the world. Its demand and price tend to increase and have brought about over exploitation of agarwood. Consequently, its population in nature has decreased significantly. To overcome the situation, since 1995, agarwood has been included in the CITES Appendix II. However, illegal exploitation remains persistent and reaches an excessive level. In order to deal with it, agarwood cultivation and its artificial production have been undertaken at several provinces in Indonesia. Some supporting factors for agarwood cultivation and artificial production are the availability of potential land for extensive cultivation, appropriate agro climate condition, simple cultivation technique and already being well adopted by farmers, the availability of necessary pathogen for agarwood inoculation, and the increasing demand with relatively high price. The research aims to analyze the feasibility study of agarwood inoculation business at several stem diameters (15 - 25 cm; 26 -35 cm and 36 - 40 cm) and periods of inoculation (1 - 5 years). Data were collected through field observation and literature study. The results showed that inoculation on agarwood producer tree stands at 12.5% interest rate afforded positive net present value (NPV), internal rate of return (IRR) is much higher than market interest and benefit cost (B/C) ratio >2 for those three diameter classes. Furthermore, if agarwood harvesting is delayed until five years after inoculation, NPV, IRR and B/C ratio would be much higher. It can be concluded that inoculation on agarwood producer tree stands (at appropriate age for inoculation) is feasible to be developed

Phylogenetic relatedness of several agarwood-producing taxa (Thymelaeaceae) from Indonesia

Indonesia is home to several tree taxa that are harvested for agarwood. This highly valuable oleoresin ironically was the cause for some species to become vulnerable due to gluttonous human activity. However, information on the genetic diversity of these endangered trees is limited. In this study, 28 specimens representing eight species from two genera, Aquilaria and Gyrinops, were collected from ex-situ and in-situ populations in Indonesia. Phylogenetic analysis conducted on DNA sequences of the nuclear ribosomal internal transcribed spacer (ITS) and the trnL-trnF intergenic spacer regions, revealed that Aquilaria and Gyrinops are paraphyletic when Aquilaria cumingiana is excluded. The phylogenetic analysis for ITS and trnL-trnF showed capability to categorise agarwoodproducing species based on their regions: East Indonesia and West Indonesia, using Wallace’s Line as the divider. In addition, we discuss challenges in species identification and taxonomy of agarwood-producing genera, and their conservation efforts in Indonesia

Utilization of Arbuscular Mycorrhizal Fungi and Coconut Shell Charcoal to Increase Gmelina arborea Roxb. and Ochroma bicolor Rowlee. Seedlings Growth in the Nursery

Latosol has spread quite widely in Indonesia, but this soil was low fertility. The success rate of planting in latosol can be supported by species selection and high quality seedling. The improvement of seedlings quality can be carried out by the application of arbuscular mycorrhizal fungi (AMF) and coconut shell charcoal. The research was conducted in a greenhouse used split plot design with a completely randomized design. The results showed that the inoculations of Gigaspora sp give the best growth on G. Arborea seedling, while the inoculation of Glomus sp. Comined with charcoal 20 % give the best growth on O. Bicolor. . In general, inoculation of plant by Gigaspora sp. provided better response than Glomus sp.

Utilization of Arbuscular Mycorrhizal Fungi (AMF) and Coconut Shell Charcoal to Increase Growth of Falcataria moluccana (Miq) Barneby & JW Grimes) and Samanea saman (Jacq) Merr Seedlings

Latosol soil has low fertility and widely spread out in Indonesia. The successful   of planting activity on latosol soil can be supported by appropiate species selection and seedling good quality. The use of arbuscular mycorrhizal fungi (AMF) and  coconut shell charcoal  for improving sengon  (Falcataria moluccana (Miq) Barneby & JW Grimes) and trembesi (Samanea saman ( Jacq) Merr growth is one alternative to support planting activities in latosol soil . This research was conducted in green house and used Split-Plot design of Complete Randomized Design (CRD) with AMF as  a main plot and coconut shell charcoal as a sub-plot. Percentage of FMA colonisation, height, diameter, crown dry weight, root dry weight, ratio of crown root and quality index of seedlings were recorded. Results showed that interaction between AMF and coconut shell charcoal increased parameters growth  for both seedling.   Single factor from both AMF  species (Glomus sp. and Gigaspora sp.) gives similar effect on growth of both seedling species

Root-induced Changes in the Rhizosphere of Extreme High Yield Tropical Rice: 1. Soil Chemical Properties

Root-induced Changes in the Rhizosphere of Extreme High Yield Tropical Rice: 1. Soil Chemical Properties     (E Purnomo,  M Turjaman, A Hairani, A Mursyid, D Choiron, R Yulia and M Osaki): Padi Panjang cultivar is one of many local rice cultivars found in South Kalimantan that yields 8 Mg ha-1 without fertilizer after last transplanting. The mechanisms involved in sustaining nutrient supply to sustain the extreme high yield are of interest. The following work aims to investigate the changes of soil chemical properties in rizhosphere of Padi Panjang cultivar. The Padi Panjang cultivar was grown in a rhizobox filled with soils from 3 different villages in Banjar Regency, South Kalimantan Province, namely, Kuin, Bunipah and Guntung Papuyu. The rice plant was grown for 5 weeks. At the end of the growing period, soil chemical properties such as pH, aluminum (Al), phosphorus (P), potassium (K+), ammonium (NH4+), and nitrate (NO3-) were measured. The results showed that Padi Panjang cultivar had the capability to change the soil chemical properties in the rhizophere. The impact was more extent compared with IR64 cultivar. The changes were depended on soil character, especially, soil texture. The soil from Guntung Papuyu was the least affected by root. It was observed that Padi Panjang cultivar acidified more than IR64. A depletion zone of K+ and NH4+ was found in the rhizosphere of both Padi Panjang and IR64 cultivars. The depletion zone of these ions could reach as far as 3 cm from the rhizosphere. For P, the depletion zone only occurred in the rhizosphere soil of IR64 cultivar. However, for Padi Panjang cultivar, the depletion zone of P did not exist. The Padi Panjang cultivar was able to maintain P concentration the same as or higher than control soil without plant. This is the first report showing that Padi Panjang cultivar can be considered as efficient lowland rice cultivar in absorbing not only P but also K in a P- and K-deficient-soil