1,503 research outputs found
Structural reorganisation of cellulose fibrils in hydrothermally deconstructed lignocellulosic biomass and relationships with enzyme digestibility
Background: The investigation of structural organisation in lignocellulose materials is important to understand
changes in cellulase accessibility and reactivity resulting from hydrothermal deconstruction, to allow development
of strategies to maximise bioethanol process efficiencies. To achieve progress, wheat straw lignocellulose and
comparative model wood cellulose were characterised following increasing severity of hydrothermal treatment.
Powder and fibre wide-angle X-ray diffraction techniques were employed (WAXD), complemented by enzyme
kinetic measurements up to high conversion.
Results: Evidence from WAXD indicated that cellulose fibrils are not perfectly crystalline. A reduction in fibril
crystallinity occurred due to hydrothermal treatment, although dimensional and orientational data showed that
fibril coherency and alignment were largely retained. The hypothetical inter-fibril spacing created by hydrothermal
deconstruction of straw was calculated to be insufficient for complete access by cellulases, although total digestion
of cellulose in both treated straw and model pulp was observed. Both treated straw and model pulps were
subjected to wet mechanical attrition, which caused separation of smaller fibril aggregates and fragments,
significantly increasing enzyme hydrolysis rate. No evidence from WAXD measurements was found for preferential
hydrolysis of non-crystalline cellulose at intermediate extent of digestion, for both wood pulp and hydrothermally
treated straw.
Conclusions: The increased efficiency of enzyme digestion of cellulose in the lignocellulosic cell wall following
hydrothermal treatment is a consequence of the improved fibril accessibility due to the loss of hemicellulose and
disruption of lignin. However, incomplete accessibility of cellulase at the internal surfaces of fibrillar aggregates
implies that etching type mechanisms will be important in achieving complete hydrolysis. The reduction in
crystalline perfection following hydrothermal treatment may lead to an increase in fibril reactivity, which could
amplify the overall improvement in rate of digestion due to accessibility gains. The lack of preferential digestion of
non-crystalline cellulose is consistent with the existence of localised conformational disorder, at surfaces and
defects, according to proposed semicrystalline fibril models. Cellulases may not interact in a fully selective manner
with such disordered environments, so fibril reactivity may be considered as a function of average conformational
states
Impact of altered cell wall composition on saccharification efficiency in stem tissue of Arabidopsis RABA GTPase-deficient knockout mutants
Use of biomass for second-generation biofuel production is severely hindered by the inherent recalcitrance of the plant cell wall to digestion. Trafficking is crucial for compartmentalisation within the cell. This process is partly regulated by small Rab GTPase proteins. In particular, control of trafficking to the cell wall is regulated through the RABA clade. Manipulation of this regulatory system offers tantalising opportunities for manipulation of cell wall composition and hence recalcitrance. Trafficking-defective rabA mutants have already been shown to impact cell wall composition. To study the impacts of these mutants on cell wall digestion, we developed a saccharification process for Arabidopsis based on the hot water method. We then showed that following pre-treatment, stems from the T-DNA knockouts of the three RABA4 genes expressed in Arabidopsis stem show an increased sugar release on saccharification. These rabA4 mutants have been shown to impact the “hemicellulose-rich” fraction during cell wall fractionation. Furthermore, we go on to show that these mutant lines also show increased sugar release when subjected to saccharification without pre-treatment. Finally, we used X-ray diffraction to show that rabA4 mutants had no impact on cellulose crystallinity, thus supporting the hypothesis that the increases in saccharification were not due to alterations of the cellulose microfibrils but were a direct effect of reduced hemicellulose levels. We also present data to show that the growth characteristics of these plants were unaffected. The data obtained from these lines are most easily explained by the reported alteration in hemicellulose increasing pre-treatment efficiency
Effect of preharvest UV-C treatment of tomatoes (Solanum lycopersicon Mill.) on ripening and pathogen resistance
Treatment with UV-C of tomato fruit on the vine was conducted using a mobile unit that was designed
to be conveyed between the rows of tomato plants in a commercial glasshouse. Trusses of fruit both at
the ripe and mature green phase were treated with UV-C doses of 3 and 8 kJ/m2. Ripe fruit were picked
8 h after treatment and kept at room temperature for up to 16 d during which colour development and
texture were monitored and compared to untreated controls. Mature green fruit treated on the vine with
UV-C doses of 3 or 8 kJ/m2 showed only a slight loss in green pigmentation in contrast to the tomato colour
index (TCI) of control fruit which increased sharply 5 d after treatment. The TCI of ripe fruit treated with
UV-C at a dose of 8 kJ/m2 showed a lag of 10 d before increasing to a final value comparable to that of
untreated fruit. Fruit treated with a dose of 3 kJ/m2 did not display a lag but the increase in TCI occurred at
a lower rate than for the controls. Firmness remained higher in fruit treated with the highest UV-C dose
compared to fruit treated with the lower UV-C dose and controls. Fruit covered with UV impermeable
film on the same plants as those that had received a UV-C dose of 3 kJ/m2 had become ripe by day 6 in
a manner similar to that of the controls. By contrast, fruit from trusses adjacent to those that had been
treated with a UV-C dose of 8 kJ/m2 remained green over the same period of time. Ripe fruit treated
as described above were inoculated with spores of Penicillium digitatum after UV-C treatment and their
firmness monitored over 12 d. A dose response effect was found with fruit treated at the highest dose
remaining firmer than those treated at the lower dose and the controls
Vacuum Strength of Two Candidate Glasses for a Space Observatory
The strengths of two candidate glass types for use in a space observatory were measured. Samples of ultra-low expansion glass (ULE) and borosilicate (Pyrex) were tested in air and in vacuum at room temperature (20 degrees C) and in vacuum after being heated to 200 degrees C. Both glasses tested in vacuum showed a significant increase in strength over those tested in air. However, there was no statistical difference between the strength of samples tested in vacuum at room temperature and those tested in vacuum after heating to 200 degrees C
Developmental Abnormalities and Reduced Fruit Softening in Tomato Plants Expressing an Antisense Rab11 GTPase Gene
Concertacion y seguridad social : de la legitimidad social a la legitimidad tecnocrática
El estudio aborda la concertación, un análisis de las políticas de seguridad social constatando su evolución desde una legitimidad social a una legitimidad tecnocrática en la crisis del welfare state y en la crisis económica con particular referencia al sistema español. Desde la normalización de la acción política de los sindicatos y agentes sociales en sus diversas manifestaciones, como la participación institucional y la "legislación negociada" en la búsqueda del consenso y el negociado de intercambios en el marco político frente a la imposición unilateral. La frustración de la concertación social se produce cuando el gobierno interpreta la democracia como algo puramente aritmético de mayorías que ejercen sin más el poder, en épocas de debilidad sindical, de debilidad del estado nación, de dificultades para las políticas redistributivas, de reforzamiento de los planteamientos neoliberales. La búsqueda de legitimidad en el pacto social no se ve en estas ocasiones como necesaria sino que la legitimidad se traslada al discurso economicista y tecnocrático, no político. Se vuelve a la búsqueda de una legitimidad a través del mercado y del intercambio entre particulares.L'estudi aborda la concertació, una anàlisi de les polítiques de seguretat social constatant la seva evolució des d'una legitimitat social a una legitimitat tecnocràtica en la crisi del welfare state i en la crisi econòmica amb particular referència al sistema espanyol. Des de la normalització de l'acció política dels sindicats i agents socials en les seves diverses manifestacions, com la participació institucional i la "legislació negociada" en la cerca del consens i el negociat d'intercanvis en el marc polític enfront de la imposició unilateral. La frustració de la concertació social es produeix quan el govern interpreta la democràcia com alguna cosa purament aritmètic de majories que exerceixen sense més el poder, en èpoques de feblesa sindical, de feblesa de l'estat nació, de dificultats per a les polítiques redistributives, de reforçament dels plantejaments neoliberals. La cerca de legitimitat en el pacte social no es veu en aquestes ocasions com a necessària sinó que la legitimitat es trasllada al discurs economicista i tecnocràtic, no polític. Es torna a la cerca d'una legitimitat a través del mercat i de l'intercanvi entre particulars.The study addresses the conclusion an analysis of policies of social security noting exposing its evolution from a social legitimacy to a technocratic legitimacy in the crisis of the welfare state and the economic crisis with particular reference to the Spanish system. Since the normalization of the political action of the trade unions and social partners in its various manifestations, such as institutional participation and negotiated "legislation" in the search for consensus and the Bureau of exchanges in the political framework against the unilateral imposition. The frustration of the social agreement occurs when the Government interprets the democracy as something purely arithmetic of majorities that exercise without more power, in times of Union weakness, weakness of the State nation, of difficulties for redistributive policies, strengthening of neoliberal approaches. The search for legitimacy in the social pact is not on these occasions as necessary but that legitimacy is moved to the economistic and technocratic, non-political speech. Turns to the search for legitimacy through the market and the exchange between individuals
The Cut & Enhance method : selecting clusters of galaxies from the SDSS commissioning data
We describe an automated method, the Cut & Enhance method (CE) for detecting
clusters of galaxies in multi-color optical imaging surveys. This method uses
simple color cuts, combined with a density enhancement algorithm, to up-weight
pairs of galaxies that are close in both angular separation and color. The
method is semi-parametric since it uses minimal assumptions about cluster
properties in order to minimize possible biases. No assumptions are made about
the shape of clusters, their radial profile or their luminosity function. The
method is successful in finding systems ranging from poor to rich clusters of
galaxies, of both regular and irregular shape. We determine the selection
function of the CE method via extensive Monte Carlo simulations which use both
the real, observed background of galaxies and a randomized background of
galaxies. We use position shuffled and color shuffled data to perform the false
positive test. We have also visually checked all the clusters detected by the
CE method. We apply the CE method to the 350 deg^2 of the SDSS (Sloan Digital
Sky Survey) commissioning data and construct a SDSS CE galaxy cluster catalog
with an estimated redshift and richness for each cluster. The CE method is
compared with other cluster selection methods used on SDSS data such as the
Matched Filter (Postman et al. 1996, Kim et al. 2001), maxBCG technique (Annis
et al. 2001) and Voronoi Tessellation (Kim et al. 2001). The CE method can be
adopted for cluster selection in any multi-color imaging surveys.Comment: 62 pages, 32 figures, Accepted for publication in the Astronomical
Journal, "the CE galaxy cluster catalog can be downloaded from,
http://astrophysics.phys.cmu.edu/~tomo/ce/
Antiferromagnetic order and the structural order-disorder transition in the Cd6Ho quasicrystal approximant
International audienceIt has generally been accepted that the orientational ordering of the Cd4 tetrahedron within the Cd6 R quasicrystal approximants is kinetically inhibited for R = Ho, Er, Tm and Lu by steric constraints. Our high-resolution X-ray scattering measurements of the Cd6Ho quasicrystal approximant, however, reveal an abrupt (first-order) transition to a monoclinic structure below T S = 178 K for samples that have 'aged' at room temperature for approximately one year, reopening this question. Using X-ray resonant magnetic scattering at the Ho L 3-edge we have elucidated the nature of the antiferromagnetic ordering below T N = 8.5 K in Cd6Ho. The magnetic Bragg peaks are found at the charge forbidden H + K + L = 2n + 1 positions, referenced to the high-temperature body-centred cubic structure. In general terms, this corresponds to antiferromagnetic arrangements of the Ho moments on adjacent clusters in the unit cell as previously found for Cd6Tb
Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.
A monoclonal antibody (H17E2) recognising both placental alkaline phosphatase (PLAP) and testicular PLAP-like alkaline phosphatase was incorporated in a solid phase immunoassay. This was used to measure levels of PLAP in 257 sera from 148 patients with germ cell neoplasms of the testis. High levels of PLAP were found in all patients with active seminomas (mean 0.85 O.D.) compared to those in clinical remission (mean 0.20 O.D.) (P less than 0.0001). More importantly, changing levels of PLAP correlated with the course of disease in 79 samples from 33 patients with seminoma (P less than 0.0001). Elevated PLAP levels were also noted in patients in remission who were smokers (mean 0.32 O.D.) compared to non-smokers (mean 0.15 O.D.) (P less than 0.001). These data demonstrate that determination of PLAP levels using this sensitive immunoassay is an important new adjunct in the monitoring of the response to treatment in patients with seminoma
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