Safety evaluation of urban transit signal system based on the improved TOPIS

AbstractSafety evaluation is one of the most effective countermeasures to improve the safety in modern urban transit system. This paper now applies that method to get the order preference of safety in all fault modes in a urban transit signal system using TOPIS based entropy weight for safety evaluation. TOPSIS is based on the concept that the chosen alternative should have the shortest distance from the positive ideal solution (PIS) and the farthest distance from the negative ideal solution (NIS). Entropy weight is ascertained by entropy theory, and the subjectivity in ascertaining the weights of more factors in lower hierarchy is avoided. The evaluation result indicates that this method is easy and can be implemented as an effective method in safety evaluation of urban transit signal system

Direct Ethanol Production from Steam-Exploded Corn Stover using a Synthetic Diploid Cellulase-displaying Yeast Consortium

The assembly of functional cellulolytic enzymes was displayed using a synthetic, cell-surface engineered diploid yeast consortium. Trichoderma reesei endoglucanase II (EGII), cellobiohydrolase II (CBHII), and Aspergillus aculeatus β-glucosidase I (BGLI) were displayed as fusion proteins with the AGA2p C-terminus of a-agglutinin on the cell surface of the diploid yeast strain Saccharomyces cerevisiae Y5. The cell-surface immobilization of each enzyme was confirmed by immunofluorescence microscopy. This type of yeast consortium allowed convenient optimization of ethanol production by adjusting the combination ratios of each cell type for inducing synergy in cellulose hydrolysis. Next, the direct ethanol fermentation from steam-exploded corn stover was investigated. The optimized cellulase-displaying consortium produced 20.4 g/L ethanol from 48.4 g cellulose per liter after 72 h in the presence of a small amount of cellulase reagent (0.9 FPU/mL). These results suggested the feasibility of the cellulase-displaying yeast consortium for simultaneous saccharification and fermentation from insoluble cellulosic materials