The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology

Spectroscopy for amateur astronomers: recording, processing, analysis and interpretation

This accessible guide presents the astrophysical concepts behind astronomical spectroscopy, covering both the theory and the practical elements of recording, processing, analysing and interpreting your spectra. It covers astronomical objects, such as stars, planets, nebulae, novae, supernovae, and events such as eclipses and comet passages. Suitable for anyone with only a little background knowledge and access to amateur-level equipment, the guide's many illustrations, sketches and figures will help you understand and practise this scientifically important and growing field of amateur astronomy, up to the level of Pro-Am collaborations. Accessible to non-academics, it benefits many groups from novices and learners in astronomy clubs, to advanced students and teachers of astrophysics. This volume is the perfect companion to the Spectral Atlas for Amateur Astronomers, which provides detailed commented spectral profiles of more than 100 astronomical objects

The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

International audienceDigital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology