104 research outputs found
tRNA splicing
Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is a crucial step in gene expression. Interestingly, even within Eukarya there are at least four splicing mechanisms. mRNA splicing in the nucleus takes place in two phosphotransfer reactions on a complex and dynamic machine, the spliceosome. This reaction is related in mechanism to the two self-splicing mechanisms for Group 1 and Group 2 introns. In fact the Group 2 introns are spliced by an identical mechanism to mRNA splicing, although there is no general requirement for either proteins or co-factors. Thus it seems likely that the Group 2 and nuclear mRNA splicing reactions have diverged from a common ancestor. tRNA genes are also interrupted by introns, but here the splicing mechanism is quite different because it is catalyzed by three enzymes, all proteins and with an intrinsic requirement for ATP hydrolysis.
tRNA splicing occurs in all three major lines of descent, the Bacteria, the Archaea, and the Eukarya. In bacteria the introns are self-splicing (1-3). Until recently it was thought that the mechanisms of tRNA splicing in Eukarya and Archaea were unrelated as well. In the past year, however, it has been found that the first enzyme in the tRNA splicing pathway, the tRNA endonuclease, has been conserved in evolution since the divergence of the Eukarya and the Archaea. Surprising insights have been obtained by comparison of the structures and mechanisms of tRNA endonuclease from these two divergent lines
Bayesian Calibrated Significance Levels Applied to the Spectral Tilt and Hemispherical Asymmetry
Bayesian model selection provides a formal method of determining the level of
support for new parameters in a model. However, if there is not a specific
enough underlying physical motivation for the new parameters it can be hard to
assign them meaningful priors, an essential ingredient of Bayesian model
selection. Here we look at methods maximizing the prior so as to work out what
is the maximum support the data could give for the new parameters. If the
maximum support is not high enough then one can confidently conclude that the
new parameters are unnecessary without needing to worry that some other prior
may make them significant. We discuss a computationally efficient means of
doing this which involves mapping p-values onto upper bounds of the Bayes
factor (or odds) for the new parameters. A p-value of 0.05 ()
corresponds to odds less than or equal to 5:2 which is below the `weak' support
at best threshold. A p-value of 0.0003 () corresponds to odds of
less than or equal to 150:1 which is the `strong' support at best threshold.
Applying this method we find that the odds on the scalar spectral index being
different from one are 49:1 at best. We also find that the odds that there is
primordial hemispherical asymmetry in the cosmic microwave background are 9:1
at best.Comment: 5 pages. V2: clarifying comments added in response to referee report.
Matches version to appear in MNRA
Backstreaming ions at a high Mach number interplanetary shock: Solar Orbiter measurements during the nominal mission phase
Solar Orbiter, a mission developed by the European Space Agency, explores in
situ plasma across the inner heliosphere while providing remote-sensing
observations of the Sun. Our study examines particle observations for the 30
October 2021 shock. The particles provide clear evidence of ion reflection up
to several minutes upstream of the shock. Additionally, the magnetic and
electric field observations contain complex electromagnetic structures near the
shock, and we aim to investigate how they are connected to ion dynamics. The
main goal of this study is to advance our understanding of the complex coupling
between particles and the shock structure in high Mach number regimes of
interplanetary shocks. We used observations of magnetic and electric fields,
probe-spacecraft potential, and thermal and energetic particles to characterize
the structure of the shock front and particle dynamics. Furthermore, ion
velocity distribution functions were used to study reflected ions and their
coupling to the shock. To determine shock parameters and study waves, we used
several methods, including cold plasma theory, singular-value decomposition,
minimum variance analysis, and shock Rankine-Hugoniot relations. To support the
analysis and interpretation of the experimental data, test-particle analysis,
and hybrid particle in-cell simulations were used. The ion velocity
distribution functions show clear evidence of particle reflection in the form
of backstreaming ions several minutes upstream. The shock structure has complex
features at the ramp and whistler precursors. The backstreaming ions may be
modulated by the complex shock structure, and the whistler waves are likely
driven by gyrating ions in the foot. Supra-thermal ions up to 20 keV were
observed, but shock-accelerated particles with energies above this were not
Observational constraints on the curvaton model of inflation
Simple curvaton models can generate a mixture of of correlated primordial
adiabatic and isocurvature perturbations. The baryon and cold dark matter
isocurvature modes differ only by an observationally null mode in which the two
perturbations almost exactly compensate, and therefore have proportional
effects at linear order. We discuss the CMB anisotropy in general mixed models,
and give a simple approximate analytic result for the large scale CMB
anisotropy. Working numerically we use the latest WMAP observations and a
variety of other data to constrain the curvaton model. We find that models with
an isocurvature contribution are not favored relative to simple purely
adiabatic models. However a significant primordial totally correlated baryon
isocurvature perturbation is not ruled out. Certain classes of curvaton model
are thereby ruled out, other classes predict enough non-Gaussianity to be
detectable by the Planck satellite. In the appendices we review the relevant
equations in the covariant formulation and give series solutions for the
radiation dominated era.Comment: Minor changes and corrections to match version accepted by PR
Statistical coverage for supersymmetric parameter estimation: a case study with direct detection of dark matter
Models of weak-scale supersymmetry offer viable dark matter (DM) candidates.
Their parameter spaces are however rather large and complex, such that pinning
down the actual parameter values from experimental data can depend strongly on
the employed statistical framework and scanning algorithm. In frequentist
parameter estimation, a central requirement for properly constructed confidence
intervals is that they cover true parameter values, preferably at exactly the
stated confidence level when experiments are repeated infinitely many times.
Since most widely-used scanning techniques are optimised for Bayesian
statistics, one needs to assess their abilities in providing correct confidence
intervals in terms of the statistical coverage. Here we investigate this for
the Constrained Minimal Supersymmetric Standard Model (CMSSM) when only
constrained by data from direct searches for dark matter. We construct
confidence intervals from one-dimensional profile likelihoods and study the
coverage by generating several pseudo-experiments for a few benchmark sets of
pseudo-true parameters. We use nested sampling to scan the parameter space and
evaluate the coverage for the benchmarks when either flat or logarithmic priors
are imposed on gaugino and scalar mass parameters. The sampling algorithm has
been used in the configuration usually adopted for exploration of the Bayesian
posterior. We observe both under- and over-coverage, which in some cases vary
quite dramatically when benchmarks or priors are modified. We show how most of
the variation can be explained as the impact of explicit priors as well as
sampling effects, where the latter are indirectly imposed by physicality
conditions. For comparison, we also evaluate the coverage for Bayesian credible
intervals, and observe significant under-coverage in those cases.Comment: 30 pages, 5 figures; v2 includes major updates in response to
referee's comments; extra scans and tables added, discussion expanded, typos
corrected; matches published versio
Ataluren stimulates ribosomal selection of near-cognate tRNAs to promote nonsense suppression
A premature termination codon (PTC) in the ORF of an mRNA generally leads to production of a truncated polypeptide, accelerated degradation of the mRNA, and depression of overall mRNA expression. Accordingly, nonsense mutations cause some of the most severe forms of inherited disorders. The small-molecule drug ataluren promotes therapeutic nonsense suppression and has been thought to mediate the insertion of near-cognate tRNAs at PTCs. However, direct evidence for this activity has been lacking. Here, we expressed multiple nonsense mutation reporters in human cells and yeast and identified the amino acids inserted when a PTC occupies the ribosomal A site in control, ataluren-treated, and aminoglycoside-treated cells. We find that ataluren\u27s likely target is the ribosome and that it produces full-length protein by promoting insertion of near-cognate tRNAs at the site of the nonsense codon without apparent effects on transcription, mRNA processing, mRNA stability, or protein stability. The resulting readthrough proteins retain function and contain amino acid replacements similar to those derived from endogenous readthrough, namely Gln, Lys, or Tyr at UAA or UAG PTCs and Trp, Arg, or Cys at UGA PTCs. These insertion biases arise primarily from mRNA:tRNA mispairing at codon positions 1 and 3 and reflect, in part, the preferred use of certain nonstandard base pairs, e.g., U-G. Ataluren\u27s retention of similar specificity of near-cognate tRNA insertion as occurs endogenously has important implications for its general use in therapeutic nonsense suppression
Cognitive bias modification for paranoia (CBM-pa): a randomised controlled feasibility study in patients with distressing paranoid beliefs
Background: Cognitive Bias Modification for paranoia (CBM-pa) is a novel, theory-driven psychological intervention targeting the biased interpretation of emotional ambiguity associated with paranoia. Study objectives were (i) test the intervention's feasibility, (ii) provide effect size estimates, (iii) assess dose-response and (iv) select primary outcomes for future trials.
Methods: In a double-blind randomised controlled trial, sixty-three outpatients with clinically significant paranoia were randomised to either CBM-pa or an active control (text reading) between April 2016 and September 2017. Patients received one 40 min session per week for 6 weeks. Assessments were given at baseline, after each interim session, post-treatment, and at 1- and 3-months post-treatment.
Results: A total of 122 patients were screened and 63 were randomised. The recruitment rate was 51.2%, with few dropouts (four out of 63) and follow-up rates were 90.5% (1-month) and 93.7% (3-months). Each session took 30-40 min to complete. There was no statistical evidence of harmful effects of the intervention. Preliminary data were consistent with efficacy of CBM-pa over text-reading control: patients randomised to the intervention, compared to control patients, reported reduced interpretation bias (d = -0.48 to -0.76), improved symptoms of paranoia (d = -0.19 to -0.38), and lower depressed and anxious mood (d = -0.03 to -0.29). The intervention effect was evident after the third session.
Conclusions: CBM-pa is feasible for patients with paranoia. A fully powered randomised control trial is warranted
Correlated perturbations from inflation and the cosmic microwave background
We compare the latest cosmic microwave background data with theoretical
predictions including correlated adiabatic and CDM isocurvature perturbations
with a simple power-law dependence. We find that there is a degeneracy between
the amplitude of correlated isocurvature perturbations and the spectral tilt. A
negative (red) tilt is found to be compatible with a larger isocurvature
contribution. Estimates of the baryon and CDM densities are found to be almost
independent of the isocurvature amplitude. The main result is that current
microwave background data do not exclude a dominant contribution from CDM
isocurvature fluctuations on large scales.Comment: 5 pages, revtex, 3 figures. V3 - DASI data added and reionization
taken into account. New figure 2. Matches version to appear in PR
Cosmological parameters from SDSS and WMAP
We measure cosmological parameters using the three-dimensional power spectrum
P(k) from over 200,000 galaxies in the Sloan Digital Sky Survey (SDSS) in
combination with WMAP and other data. Our results are consistent with a
``vanilla'' flat adiabatic Lambda-CDM model without tilt (n=1), running tilt,
tensor modes or massive neutrinos. Adding SDSS information more than halves the
WMAP-only error bars on some parameters, tightening 1 sigma constraints on the
Hubble parameter from h~0.74+0.18-0.07 to h~0.70+0.04-0.03, on the matter
density from Omega_m~0.25+/-0.10 to Omega_m~0.30+/-0.04 (1 sigma) and on
neutrino masses from <11 eV to <0.6 eV (95%). SDSS helps even more when
dropping prior assumptions about curvature, neutrinos, tensor modes and the
equation of state. Our results are in substantial agreement with the joint
analysis of WMAP and the 2dF Galaxy Redshift Survey, which is an impressive
consistency check with independent redshift survey data and analysis
techniques. In this paper, we place particular emphasis on clarifying the
physical origin of the constraints, i.e., what we do and do not know when using
different data sets and prior assumptions. For instance, dropping the
assumption that space is perfectly flat, the WMAP-only constraint on the
measured age of the Universe tightens from t0~16.3+2.3-1.8 Gyr to
t0~14.1+1.0-0.9 Gyr by adding SDSS and SN Ia data. Including tensors, running
tilt, neutrino mass and equation of state in the list of free parameters, many
constraints are still quite weak, but future cosmological measurements from
SDSS and other sources should allow these to be substantially tightened.Comment: Minor revisions to match accepted PRD version. SDSS data and ppt
figures available at http://www.hep.upenn.edu/~max/sdsspars.htm
CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration
SummaryNeurodegenerative diseases can occur so early as to affect neurodevelopment. From a cohort of more than 2,000 consanguineous families with childhood neurological disease, we identified a founder mutation in four independent pedigrees in cleavage and polyadenylation factor I subunit 1 (CLP1). CLP1 is a multifunctional kinase implicated in tRNA, mRNA, and siRNA maturation. Kinase activity of the CLP1 mutant protein was defective, and the tRNA endonuclease complex (TSEN) was destabilized, resulting in impaired pre-tRNA cleavage. Germline clp1 null zebrafish showed cerebellar neurodegeneration that was rescued by wild-type, but not mutant, human CLP1 expression. Patient-derived induced neurons displayed both depletion of mature tRNAs and accumulation of unspliced pre-tRNAs. Transfection of partially processed tRNA fragments into patient cells exacerbated an oxidative stress-induced reduction in cell survival. Our data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans
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