Cytonuclear interactions remain stable during allopolyploid evolution despite repeated whole-genome duplications in Brassica

Several plastid macromolecular protein complexes are encoded by both nuclear and plastid genes. Therefore, cytonuclear interactions are held in place to prevent genomic conflicts that may lead to incompatibilities. Allopolyploidy resulting from hybridization and genome doubling of two divergent species can disrupt these fine-tuned interactions, as newly formed allopolyploid species confront biparental nuclear chromosomes with a uniparentally inherited plastid genome. To avoid any deleterious effects of unequal genome inheritance, preferential transcription of the plastid donor over the other donor has been hypothesized to occur in allopolyploids. We used Brassica as a model to study the effects of paleopolyploidy in diploid parental species, as well as the effects of recent and ancient allopolyploidy in Brassica napus, on genes implicated in plastid protein complexes. We first identified redundant nuclear copies involved in those complexes. Compared with cytosolic protein complexes and with genome-wide retention rates, genes involved in plastid protein complexes show a higher retention of genes in duplicated and triplicated copies. Those redundant copies are functional and are undergoing strong purifying selection. We then compared transcription patterns and sequences of those redundant gene copies between resynthesized allopolyploids and their diploid parents. The neopolyploids showed no biased subgenome expression or maternal homogenization via gene conversion, despite the presence of some non-synonymous substitutions between plastid genomes of parental progenitors. Instead, subgenome dominance was observed regardless of the maternal progenitor. Our results provide new insights on the evolution of plastid protein complexes that could be tested and generalized in other allopolyploid species

Assessment of pollen size according to ploidy level in Brassica model.

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Assessing the impact of the hydraulic conductivity of a crusted soil on overland flow modelling at the field scale

Correspondance: [email protected] audienceSoil surface crusts are widely reported to favour Hortonian runoff, but are not explicitly represented in most rainfall-runoff models. The aim of this paper is to assess the impact of soil surface crusts on infiltration and runoff modelling at two spatial scales, i.e. the local scale and the plot scale. At the local scale, two separate single ring infiltration experiments are undertaken. The first is performed on the undisturbed soil, whereas the second is done after removal of the soil surface crust. The HYDRUS 2D two-dimensional vertical infiltration model is then used in an inverse modelling approach, first to estimate the soil hydraulic properties of the crust and the subsoil, and then the effective hydraulic properties of the soil represented as a single uniform layer. The results show that the crust hydraulic conductivity is 10 times lower than that of the subsoil, thus illustrating the limiting role the crust has on infiltration. Moving up to the plot scale, a rainfall-runoff model coupling the Richards equation to a transfer function is used to simulate Hortonian overland flow hydrographs. The previously calculated hydraulic properties are used, and a comparison is undertaken between a single-layer and a double-layer representation of the crusted soil. The results of the rainfall-runoff model show that the soil hydraulic properties calculated at the local scale give acceptable results when used to model runoff at the plot scale directly, without any numerical calibration. Also, at the plot scale, no clear improvement of the results can be seen when using a double-layer representation of the soil in comparison with a single homogeneous layer. This is due to the hydrological characteristics of Hortonian runoff, which is triggered by a rainfall intensity exceeding the saturated hydraulic conductivity of the soil surface. Consequently, the rainfall-runoff model is more sensitive to rainfall than to the subsoil's hydrodynamic properties. Therefore, the use of a double-layer soil model to represent runoff on a crusted soil does not seem necessary, as the increase of precision in the soil discretization is not justified by a better performance of the model

Effect of deep tillage for vineyard establishment on soil structure: A case study in Southern France

International audienceDeep tillage that is used before vine plantation to remove old vine roots and loosen subsoil may induce physical soil degradation that could affect soil structure and vine water supply. The objective of the study was to experimentally evaluate the effect of deep tillage on soil structure. The impacts on soil structure of two deep tillage techniques, i.e. deep ploughing and ripper, and two contrasted soil water conditions were compared in a experimental field by combining morphological observations, bulk density and saturated hydraulic conductivity measurements. These three methods were found very complementary to analyse and discriminate the impact of the different treatments. The proportion of compacted zones and mean bulk density increased from the initial plot (0.15 m(2) m(-2), 1.45 Mg m(-3)) to a maximum in the case of the deep ploughing under wet conditions plot (0.60 m(2) m(-2), 1.60 Mg m(-3)). The main results showed that (i) a significant soil compaction was observed after wet conditions only, (ii) deep ploughing produced more soil compaction than ripper because of a greater volume of soil affected by wheeling in the former operation and (iii) a specific response of soils is significatively observed in the case of deep ploughing only with an increase of compacted zones fragmentation in relation to a decrease of clay content

Double dose efficiency of the yellow rust resistance gene Yr17 in bread wheat lines

International audienceYellow rust, caused by Puccinia striiformis f. sp. tritici, is one of the most severe wheat disease worldwide. Crop losses have ranged from 10% to 70% and up to 100% in extreme conditions. Eighty-two resistance genes, designated Yr, have been identified. Among them, Yr17 derived from Aegilops ventricosa and located on chromosome 2A has been widely used in wheat breeding. However, it had been overcome already. Through recombination of the Ae. ventricosa Yr17-carrying 6N(v) chromosome with 2D of wheat, we introduced Yr17 onto chromosome 2D. Then, lines carrying Yr17 on both 2A and 2D were generated. Seedlings of the latter, as well as those carrying a single dose of Yr17 either on 2A or on 2D, were inoculated with virulent or avirulent strains on wheat seedlings. The different genotypes were fully susceptible for the two pathotypes that are virulent on Yr17. In the case of avirulent pathotypes, the Yr17 double dose lines were fully resistant, while those with the Yr17 gene only on either 2A or 2D had intermediate resistance reactions towards one or the other or both pathotypes

The wind's evaporation method : a standard laboratory method adopted by the french INRA laboratories. 1. Technical characteristics and error analysis

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An automatic non-invasive classification for plant phenotyping by MRI images: An application for quality control on cauliflower at primary meristem stage

International audienceDuring the past few years, milder autumn and winter seasons have caused severe problems to cauliflower harvest of Brittany region in France, mainly due to curd deformation. Consequently, cauliflower breeders are working on breeding new varieties that are more robust to climate change to stabilize the quality of cauliflower production. The aim of this study was to identify at which stage of the curd formation, significant difference can be detected between healthy and stressed cauliflower. A non-invasive classification based on Magnetic Resonance Imaging (MRI) images for cauliflower phenotyping was proposed. Plants exposed to vernalization stress were sampled at different times around primary meristem stage, then both MRI imaged and apex dissected. A work flow was developped to extract features from MRI images. A classification on phenotype was learned by LDA, QDA, PLSDA and CNN binary classification between two groups: healthy and stressed cauliflower. Promising F1 score and MCC up to 95% were achieved. Curd deformation is the main cause for cauliflower’s later physiological disorders when reaching maturity. Therefore, the cauliflowers with deformation could be removed at the earliest, e.g., screening for plant breeding. At the same time, the healthy cauliflowers are not destroyed and continue their life cycl

Durable resistance to stripe rust is due to three specific resistance genes in french bread wheat cultivar Apache.

Quantitative resistance is postulated to be more durable than qualitative (R-gene mediated) resistance, which is usually quickly overcome by the pathogen population. Despite its wide use for nearly 10 years in France, the French bread wheat cultivar Apache remains resistant to stripe rust. Here, we investigated the genetic architecture of cv. Apache resistance to examine whether its durability could be explained by quantitative characteristics. We identified quantitative trait loci (QTL) by composite interval mapping of disease progress data recorded throughout 4 years of field assays. These assays included inoculation with three different pathotypes on a segregating population originating from a cross between cv. Apache and cv. Taldor, a French cultivar susceptible to stripe rust. Three QTLs derived from Apache, QYr.inra-2AS, QYr.inra-2BL and QYr.inra-4B, were detected. Each of these QTLs contributed between approximately 15 and 69 % of the phenotypic variance and corresponds to a race-specific resistance gene. We showed that QYr.inra-2AS and QYr.inra-2BS map to the positions of Yr17 and Yr7, respectively, whereas QYr.inra-4B corresponds to an adult plant resistance gene. Our results demonstrate that a combination of two or more race-specific resistance genes can confer durable resistance provided that it is properly managed at a continental level. Race-specific resistance genes should not be removed from breeding programs provided that they are properly managed

Extracting the A genome from oilseed rape

International audienc

Shift to self-compatibility in synthetic allopolyploid Brassica napus

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