Isobar-free neon isotope measurements of flux-fused potential reference minerals on a Helix-MC-Plus^(10K) mass spectrometer

This work presents new analytical techniques for extraction and analysis of neon from a suite of different mineral phases, including quartz, pyroxene, hematite, apatite, zircon, topaz, and fluorite. Neon was quantitatively extracted at 1100 °C from all of these minerals using an in-vacuum lithium borate-flux fusion technique. Evolved neon was purified using a cryogenic method capable of separating Ne from He present in abundances ~8 orders of magnitude higher, typical of samples carrying nucleogenic/radiogenic noble gases. The purified neon was measured on a Helix-MC-Plus^(10K) mass spectrometer that permits isobar-free measurement of all three neon isotopes. When operated at its highest mass resolving power (MRP) of ~10,300, the shoulder representing solely ²²Ne on the low mass-side of the ²²Ne-CO₂⁺² doublet is wide enough to permit measurement of isobar free ²²Ne. Operating in this mode comes with the penalty of a 50% reduction in neon sensitivity. Coupled with a mathematical isobar-stripping method, this approach excludes 99.5% of the CO₂⁺² while still collecting >99% of the ²²Ne beam. Routine edge-centering on the dynamic CO₂⁺² peak prior to introduction of a sample permits rapid and robust relocation of the desired measure point in the mass spectrum. Cosmogenic ²¹Ne and ²²Ne concentrations obtained using these methods on the Cronus-A quartz and Cronus-P pyroxene international reference materials are in excellent agreement with previous work or expectations. Similarly, the concentration of nucleogenic ²¹Ne and ²²Ne in Durango apatite and the CIT hematite standard agree well with previous work. Durango apatite has notable heterogeneity in neon concentrations, consistent with previous observations of heterogeneous He, U and Th concentrations in this apatite. Nucleogenic neon concentrations are also presented for previously unstudied minerals including a Sri Lanka zircon (SLC), a topaz from the Imperial Topaz mine in Brazil (ITP1), and a fluorite (W-90) from New Hampshire. Taken together this set of potential reference minerals and the associated dataset provide a starting point for intercalibration among multiple mineral phases carrying ²¹Ne and ²²Ne of cosmogenic or nucleogenic origin

Isobar-free neon isotope measurements of flux-fused potential reference minerals on a Helix-MC-Plus^(10K) mass spectrometer

This work presents new analytical techniques for extraction and analysis of neon from a suite of different mineral phases, including quartz, pyroxene, hematite, apatite, zircon, topaz, and fluorite. Neon was quantitatively extracted at 1100 °C from all of these minerals using an in-vacuum lithium borate-flux fusion technique. Evolved neon was purified using a cryogenic method capable of separating Ne from He present in abundances ~8 orders of magnitude higher, typical of samples carrying nucleogenic/radiogenic noble gases. The purified neon was measured on a Helix-MC-Plus^(10K) mass spectrometer that permits isobar-free measurement of all three neon isotopes. When operated at its highest mass resolving power (MRP) of ~10,300, the shoulder representing solely ²²Ne on the low mass-side of the ²²Ne-CO₂⁺² doublet is wide enough to permit measurement of isobar free ²²Ne. Operating in this mode comes with the penalty of a 50% reduction in neon sensitivity. Coupled with a mathematical isobar-stripping method, this approach excludes 99.5% of the CO₂⁺² while still collecting >99% of the ²²Ne beam. Routine edge-centering on the dynamic CO₂⁺² peak prior to introduction of a sample permits rapid and robust relocation of the desired measure point in the mass spectrum. Cosmogenic ²¹Ne and ²²Ne concentrations obtained using these methods on the Cronus-A quartz and Cronus-P pyroxene international reference materials are in excellent agreement with previous work or expectations. Similarly, the concentration of nucleogenic ²¹Ne and ²²Ne in Durango apatite and the CIT hematite standard agree well with previous work. Durango apatite has notable heterogeneity in neon concentrations, consistent with previous observations of heterogeneous He, U and Th concentrations in this apatite. Nucleogenic neon concentrations are also presented for previously unstudied minerals including a Sri Lanka zircon (SLC), a topaz from the Imperial Topaz mine in Brazil (ITP1), and a fluorite (W-90) from New Hampshire. Taken together this set of potential reference minerals and the associated dataset provide a starting point for intercalibration among multiple mineral phases carrying ²¹Ne and ²²Ne of cosmogenic or nucleogenic origin

Sampling interplanetary dust from Antarctic air

We built a collector to filter interplanetary dust particles (IDPs) larger than 5 μm from the clean air at the Amundsen Scott South Pole station. Our sampling strategy used long duration, continuous dry filtering of near‐surface air in place of short duration, high‐speed impact collection on flags flown in the stratosphere. We filtered ~10$^7$ m$^3$ of clean Antarctic air through 20 cm diameter, 3 µm filters coupled to a suction blower of modest power consumption (5–6 kW). Our collector ran continuously for 2 years and yielded 41 filters for analyses. Based on stratospheric concentrations, we predicted that each month’s collection would provide 300–900 IDPs for analysis. We identified 19 extraterrestrial (ET) particles on the 66 cm$^2$ of filter examined, which represented ~0.5% of the exposed filter surfaces. The 11 ET particles larger than 5 µm yield about a fifth of the expected flux based on >5 µm stratospheric ET particle flux. Of the 19 ET particles identified, four were chondritic porous IDPs, seven were FeNiS beads, two were FeNi grains, and six were chondritic material with FeNiS components. Most were <10 µm in diameter and none were cluster particles. Additionally, a carbon‐rich candidate particle was found to have a small $^{15}$N isotopic enrichment, supporting an ET origin. Many other candidate grains, including chondritic glasses and C‐rich particles with Mg and Si and FeS grains, require further analysis to determine if they are ET. The vast majority of exposed filter surfaces remain to be examined

Appendix A. Study site and soil analysis methods.

Study site and soil analysis methods

Appendix C. Additional statistical analyses assessing relationships between enzymes, sites, and soil characteristics.

Additional statistical analyses assessing relationships between enzymes, sites, and soil characteristics

Appendix B. Additional soil biogeochemical data.

Additional soil biogeochemical data

Appendix D. Additional figures illustrating relationships between enzymes and soil characteristics.

Additional figures illustrating relationships between enzymes and soil characteristics

Oncostatin M produced in Kupffer cells in response to PGE2: possible contributor to hepatic insulin resistance and steatosis

Hepatic insulin resistance is a major contributor to hyperglycemia in metabolic syndrome and type II diabetes. It is caused in part by the low-grade inflammation that accompanies both diseases, leading to elevated local and circulating levels of cytokines and cyclooxygenase (COX) products such as prostaglandin E2 (PGE2). In a recent study, PGE2 produced in Kupffer cells attenuated insulin-dependent glucose utilization by interrupting the intracellular signal chain downstream of the insulin receptor in hepatocytes. In addition to directly affecting insulin signaling in hepatocytes, PGE2 in the liver might affect insulin resistance by modulating cytokine production in non-parenchymal cells. In accordance with this hypothesis, PGE2 stimulated oncostatin M (OSM) production by Kupffer cells. OSM in turn attenuated insulin-dependent Akt activation and, as a downstream target, glucokinase induction in hepatocytes, most likely by inducing suppressor of cytokine signaling 3 (SOCS3). In addition, it inhibited the expression of key enzymes of hepatic lipid metabolism. COX-2 and OSM mRNA were induced early in the course of the development of non-alcoholic steatohepatitis (NASH) in mice. Thus, induction of OSM production in Kupffer cells by an autocrine PGE2-dependent feed-forward loop may be an additional, thus far unrecognized, mechanism contributing to hepatic insulin resistance and the development of NASH

Inhibition of COX 1 and 2 prior to Renal Ischemia/Reperfusion Injury Decreases the Development of Fibrosis

Ischemia and reperfusion injury (IRI) contributes to the development of chronic interstitial fibrosis/tubular atrophy in renal allograft patients. Cyclooxygenase (COX) 1 and 2 actively participate in acute ischemic injury by activating endothelial cells and inducing oxidative stress. Furthermore, blockade of COX 1 and 2 has been associated with organ improvement after ischemic damage. The aim of this study was to evaluate the role of COX 1 and 2 in the development of fibrosis by performing a COX 1 and 2 blockade immediately before IRI. We subjected C57Bl/6 male mice to 60 min of unilateral renal pedicle occlusion. Prior to surgery mice were either treated with indomethacin (IMT) at days –1 and 0 or were untreated. Blood and kidney samples were collected 6 wks after IRI. Kidney samples were analyzed by real-time reverse transcription–polymerase chain reaction for expression of transforming growth factor β (TGF-β), monocyte chemoattractant protein 1 (MCP-1), osteopontin (OPN), tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-10, heme oxygenase 1 (HO-1), vimentin, connective-tissue growth factor (CTGF), collagen I, and bone morphogenic protein 7 (BMP-7). To assess tissue fibrosis we performed morphometric analyses and Sirius red staining. We also performed immunohistochemical analysis of anti-actin smooth muscle. Renal function did not significantly differ between groups. Animals pretreated with IMT showed significantly less interstitial fibrosis than nontreated animals. Gene transcript analyses showed decreased expression of TGF-β, MCP-1, TNF-α, IL-1-β, vimentin, collagen I, CTGF, and IL-10 mRNA (all P < 0.05). Moreover, HO-1 mRNA was increased in animals pretreated with IMT (P < 0.05). Conversely, IMT treatment decreased osteopontin expression and enhanced BMP-7 expression, although these levels did not reach statistical significance when compared with control expression levels. The blockade of COX 1 and 2 resulted in less tissue fibrosis, which was associated with a decrease in proinflammatory cytokines and enhancement of the protective cellular response