Radioprotective action of venom of honey bee Apis mellifera Caucasica

The paper presents experimental data on the influence of the product of the activity of the honey bee Apis mellifera Caucasica on the life span of experimental animals irradiated with small doses of gamma radiation. The aim of the studies was to study the radioprotective effect of the pre-introduced zootoxin Apis mellifera Caucasica with a single gamma irradiation of 60Co mice at doses of 1.3, 5, 7, 10 Gy at irradiation dose rates of 1 Gr / min. Injection of venom followed by gamma irradiation of 60Co at a dose of D = 1, 3, 5 and 7 Gy at an irradiation dose rate of 1 Gy / min increased the life span of the experimental groups of mice ranging from 45% to 56 % and from 52% to 67%, respectively. An increase in the lifespan of experimental animals exposed to radiation with the preliminary introduction of the venom of the honey bee Apis mellifera Caucasica was revealed

Study of the Activity of Phospholipase A2 in Venom of the Transcaucasian Macrovipera Lebetina Obtusa

The activity of phospholipase A2 in the venom of the Transcaucasian Macrovipera lebetina obtusa, collected from viperas inhabiting in different regions of Azerbaijan in terms of pollution, was studied. The lowest (30,2 IU / mg) was detected in the venom of Viperas collected in Sabirabad district, Karatuga village and the highest was detected in the venom of Viperas collected in Baku, s. Bina (38,5IU / mg). As a result of experimental studies it follows that the activity of phospholipase in the venom collected in the vicinity of the Sabirabad district Karatuga village and Agsu district Garagoyunlu village, Gobustan district Childag village, Bina and Sumgait is 30.2 ± 1.1 IU / mg, 32.6 ± 0.9 IU / mg, 34.5 ± 0.8 IU / mg, 38.5 ± 0.2 IU / mg and 36.1 ± 0 , 8 IU / mg, respectively. Thus, the activity of phospholipase A2 in the venom of the Transcaucasian Viperas Macrovipera lebetina obtusa collected from snakes inhabiting different in the degree of contamination of the regions of Azerbaijan was studied. The results of the experimental data can be used to determine the biological activity of the venom samples, including, for identification and standardization of the venom of the vipera

Ecology factor and Venom of snake Macrovipera lebetina obtusa

In this article presents experimental data, the basic composition of the venom of Macrovipera lebetina obtusa, captured from different regions of Azerbaijan, differing in degree of contamination by industrial emissions was studied. γ – radiospectrometric studies showed that the samples of venom also contain radionuclides as Ra228, Ra226, K40 and 137 Cs. It was established that the radiation dose (up to dose 1.35 kGy) for 3 minutes did not cause structural changes in the samples venom of vipera, but rather contribute to the stabilization of both toxicity and pharmacological activity while increasing the shelf life of aqueous solutions of vipera venom. At high doses (2.7, 4.05 and 5.4 kGy) γ-irradiation for 3 minutes there was a gradual decrease in toxicity (pharmacological activity of enzymes) of snake venom. We can assume that these data can be used in the identification of zootoxins and their metabolites, and these criteria can serve as a theoretical basis for the development of effective methods for diagnosis of poisoning zootoxins

Investigation of the proteolytic activity of liver trematodes in goats of Khizi-Khachmaz zone of Azerbaijan

The article presents experimental data on the detection of proteolytic activity of liver trematodes in the goats of Khizi-Khachmaz zone of Azerbaijan in different seasons of the year. Determination of the enzymatic activity was carried out spectrophotometrically using a Folin reagent on a Specol 1500 spectrophotometer (Analitik Jena). The maximum peak of intensity of proteolytic activity of trematodes isolated from goat liver was detected. The maximum value of the enzyme activity was reached in March equal to 170 μg of tyrosine per gram of wet weight of the helminth, and the minimum in June reaching 70 μg of tyrosine per gram of wet weight of the helminth

Роль полиморфизма генов в чувствительности к саркоидозу легких

Authors of this review described gene polymorphism  involved in the development  of the immune  response in sarcoidosis. Gene background could determine not only the subject’s susceptibility to sarcoidosis, but also a clinical course of the disease. Currently,  allele variants that could be used as prognostic markers to reveal subjects susceptible to sarcoidosis and to predict a clinical course of sarcoidosis are investigated. Published data on a relationship between the carriage of certain allele variants and the subject’s susceptibility to sarcoidosis are scarce and controversial. Nevertheless, those data could be used to detect potential goals for targeted therapy of sarcoidosis and to develop measures to prevent this disease.В обзоре обобщены  сведения  о роли полиморфных вариантов  генов в формировании иммунного  ответа при саркоидозе.  Обозначены основные  гены-кандидаты, однонуклеотидные замены,  которые могут оказывать  модулирующее действие на развитие иммунных реакций при данной патологии, а также определять не только восприимчивость людей к возникновению саркоидоза  легких, но и оказывать влияние  на клинические характеристики течения заболевания

Influence of electromagnetic radiation on venom of Vipera lebetina obtuse

Primary stage of influence of the external physical factor are some conformative transformations of fibers-receptors which start the intensifying cascade of biochemical reactions. Thus energy of external influence can be on usages of less response of a cage. After an initial stage of absorption of the electromagnetic radiation, one of mechanisms of realisation of action of electromagnetic radiation of a microwave range on live organisms considers updating freely radicalthe reactions proceeding in cages and fabrics under the influence of electromagnetic energy. Problem of studying of influence of nonionising electromagnetic radiation is revealing and an effect explanation at level of the whole organism, at level of the central nervous system. Researches of influence of electromagnetic radiations on biosystems are spent basically in 2 frequency ranges: ultralow electromagnetic frequencies (1-20Hz) and ultrahigh electromagnetic frequencies (0.3GHz-300GHz). The greatest potential danger to biological objects radiations with frequencies which possessing getting action, influence both an organism as a whole, and on its touch and effectorive systems represent, fabrics and various reseptorive structures. In vivo and in vitro experiments with an irradiation were spent on the snakes containing in standartive conditions. Snakes (Vipera lebetina obtusа) were subdivided on two groups, each of 5 snakes. The skilled group of snakes was exposed to an irradiation in a current of 4 minutes. The skilled group of snakes was exposed to an irradiation. Radiation source was the UHF generator. Frequency of radiation has been chosen on the maximum factor of absorption of poison. The irradiation of animals was spent in the special chamber and depending on the purpose of experiments for realisation of modes high (U-14000B) and low (U-7000B) intensity of an irradiation target capacities of a radiator - P - 70Bт were applied. In a day, 2, 3, 4 and 5 days after an irradiation of snakes we took venom, dried up poison in cups Petri placed in eksikator over steams of chloride calcium. After venom drying defined fiber total on method Ƃредф°рда. Simultaneously definition of toxicity of venom was spent on white three-monthly rats. It is necessary to notice, that at an irradiation of snakes electromagnetic radiation of low intensity did not mark change of quantity of the general fiber in poison of snakes. Toxicity of venom also was not exposed to changes. At high intensity of radiation insignificant fluctuations in the maintenance of the general fiber and toxicity of venom of snakes were observed