Suitability assesment for vineyards in Kaštela-Trogir area

Analizom terenskih i laboratorijskih istraživanja utvrđeni su određeni nedostaci koji se u tlu očituju kroz nisku opskrbljenost fosforom i kalijem, povećan pH, visok sadržaj karbonata. Uz navedene nedostatke utvrđene su i pozitivne karakteristike kao što su: prisutnost skeleta različitih frakcija koji pokriva površinu vinograda i doprinosi zadržavanju vlage u tlu te potpomaže ukupnu insolaciju koja ima utjecaj na količinu šećera u samom grožđu, postotak humusa koji je unutar optimalnih 1 - 3 %, te se kreće od 1,20 - 2,80 %, količina oborina od 687,00 -1 436,00 litara koja zadovoljava potrebe vinove loze. Problem je njen raspored tijekom godine, odnosno nedostatak prilikom ljetnih mjeseci koji se rješava navodnjavanjem. Unatoč utvrđenim nedostacima, način na koji je izvedena sama priprema sadnje vinograda predstavlja novi aspekt zbrinjavanja materijala iz raznih građevinskih radova te se tako oplemenjuju površine uz racionaano odvajanje i zbrinjavanje materijala. Takvim pristupom dobivaju se nove proizvodne površine koje po svom izvornom obliku to nisu bile. Svi parametri koji su utvrđeni pokazuju kako ovakav pristup prostornog uređenja zemljišta ima potencijal te bi se svakim daljnjim radom uz prethodno stečena iskustva mogao poboljšati i unaprijediti. Nekontroliranim i neplanskim nasipavanjem dolazi do trajne i nepovratne degradacije tla, te će ovakav pristup u budućnosti doći sve više do izražaja, kako bi se izbalansirala potreba zbrinjavanja materijala i budućeg korištenja nasutog zemljišta.The analysis of field and laboratory research shows some deficiencies in the soil that are manifested through a low supply of phosphorus and potassium, increased pH, high carbonate content. In addition to these deficiencies identified were also and positive characteristics such as the presence of the rock material different fraction which is distributed by the surface of the vineyard, and which contributes to the retention of moisture in the soil and promotes overall insulation that has an impact on the amount of sugar in the grapes, the percentage of humus, which is within the optimal 1 - 3 %, and ranges from 1.20 to 2.80 %, the amount of rainfall is 687 - 1436 l and meets the needs of the vitis vinifera, the problem is her schedule during the year or during the summer months, the lack of which can be offset by irrigation. Despite the identified shortfalls, the way that is carried preparing planting vineyards represents a new aspect of dealing with waste material from a variety of construction projects and thereby enrich the surface with a rational separation and disposal of materials. Such an approach gives the new production area, which was not exits in its original form. All parameters that are determined show that this approach to zoning of land has potential and in any further work with the learned lessons, could improve and enhance. Uncontrolled and unplanned filling leads to permanent and irreversible soil degradation, and this approach will in future come increasingly to the fore, to balance the needs of housing materials and future use of the buried soil

Influence of light and hormone concentration on in vitro growth of shallot (Allium ascalonicum L.)

Rod Allium L. obuhvaća oko 750 vrsta. Unutar roda Allium nalazi se vrsta Allium cepa var. Ascalonicum L. poznata kao i ljutika (shallot). Za navedenu vrstu koriste se sinonimi: Allium cepa var. aggregatum G.Don i Allium cepa var. Ascalonicum L. Ljutika ili luk kozjak, (Allium ascalonicum) potječe s područja današnje Palestine i raširena je gotovo po cijelom Orijentu i srednjoj Aziji. Od davnina se koristi u ishrani i u narodnoj medicini. Kao i ostale vrste roda Allium ekstrakt ove vrste ima jaku antioksidativnu aktivnost i antimikrobno djelovanje Dvogodišnja je biljaka, koja se samorazmnožavanjem može održati više godina na istom mjestu. Glavna razlika između luka i ljutike je ta što ljutika ima manje lukovice koje rastu na istoj stabljici te ih može biti od 3 do 20. Cilj istraživanja je bio utvrditi utjecaj osvjetljenja i različite koncentracije hormona na početni porast eksplantata ljutike in vitro. Za istraživanje su korištene dvije različite varijante hranjive podloge (2,0 mg/L BAP i 5 mg/L BAP) te dvije varijante osvjetljenja, bijelo svjetlo i plavo svjetlo. Nakon dva tjedna uzgoja u kulturi određeni su masa, duljina i multiplikacijski indeks. Analizom varijance utvrđen je statistički značajan utjecaj (p < 0,01) koncentracije hormona na rast ljutike u prvom ciklusu. Najveća prosječna duljina i masa ljutike u prvom ciklusu izmjerena je kod tretmana bijelim svjetlom uz koncentraciju hormona 5 mg/L BAP. Analizom varijance utvrđen je statistički značajan utjecaj (p < 0,01) koncentracije hormona na rast ljutike. u drugom ciklusu. Prosječni indeks multiplikacije ljutike iznosio je 3,4133.Genus Allium L. embraces about 750 species. Within the genus Allium there is the species Allium cepa var. Ascalonicum L. Known as a Shallot. The synonym used for this species is Allium cepa var. Aggregate G.Don and Allium cepa var. Ascalonicum L. Shallot(Allium ascalonicum L.) is originates from the territory of today's Palestine and is spread almost throughout the Orient and Central Asia. Since ancient times it has been used in nutrition and in folk medicine. Like other Allium species, extract of shallot has strong antioxidant activity and antimicrobial activity. It is a two year plant, which can be sustained several years in the same place. The main difference between arch and anger is that the angel has fewer bulbs growing on the same stem and can range from 3 to 20.The aim of this study was to determine the influence of illumination and different hormone concentrations on the initial growth of in vitro excitement of the sting. Two different nutrient media variants (2.0 mg / L BAP and 5 mg / L BAP) were used for the study, and two variants of illumination, white light and blue light. After two weeks of cultivation, weight, length and multiplication index were determined. The variance analysis showed a statistically significant effect (p <0.01) of the hormone concentration on the incidence of sputum in the first cycle. The highest mean length and mass of first-cycle heart rate was measured in white light with a 5 mg/L BAP hormone concentration. The variance analysis showed a statistically significant effect (p <0.01) of the hormone concentration on the incidence of sputum. In the second cycle. The average multiplication index was 3,4133. Thesis performed at: Faculty of Agricultur

Uvođenje autohtonih ekotipova češnjaka in vitro pod utjecajem regulatora rasta i svjetlost

Garlic is valuable crop that is used not only for human consumption, but also in pharmaceutical, food and cosmetics industries. Indigenous cultivars have specific beneficial properties that could be permanently lost due to cultivation of new cultivars. Aim of this study was to established two indigenous garlic cultivars in vitro under influence of growth regulator and light. Garlic varieties Slavonian winter (Croatia) and Vincek (Slovenia) were introduced on medium supplemented with two different concentrations of BAP (1 and 1,5 mg/L) and grown under two types of light (FLUO and LED). Results showed there were no significant influence of light type but concentration of growth regulator significantly influenced in vitro development of garlic microshoots of both investigated cultivars. The best treatment for Vincek garlic explants was nutrient medium supplemented with 1.5 mg/L BAP and LED lights, while for Slavonian winter garlic the best treatment showed to be FLUO light and 1,5 mg/L BAP.Češnjak je dragocjena kultura koja se koristi osim za ljudsku prehranu, i u farmaceutskoj, prehrambenoj i kozmetičkoj industriji. Autohtone sorte imaju specifična korisna svojstva koja bi mogla biti trajno izgubljena uzgojem novih kultivara. Cilj ovog istraživanja bio je uspostaviti in vitro protokol za uzgoj dvije autohtone sorte češnjaka pod utjecajem regulatora rasta i svjetla. U in vitro kulturu uvedene su autohtone sorte češnjaka Slavonski ozimi (Hrvatska) i Vincek (Slovenija) na dvije različite varijante hranjive podloge (1 i 1,5 mg/L BAP-a) te su uzgajane pod dvije varijante osvjetljenja (FLUO i LED). Rezultati su pokazali da nije bilo značajnog utjecaja vrste svjetla, ali je koncentracija regulatora rasta značajno utjecala na in vitro razvoj mikroizdanaka češnjaka obje ispitivane sorte. Eksplantati češnjaka Vincek postigli su bolje rezultate na tretmanu hranjive podloge s dodatkom 1,5 mg/L BAP-a i LED svjetla, dok se za Slavonski ozimi češnjak pokazao najbolji tretman FLUO svjetlo i 1,5 mg/L BAP-a

Sterilizacija različitih tipova eksplantata u mikropropagaciji podloga trešnje CAB-6p i Gisela 6

Research with the objective of finding efficient (non residual) sterilants for the purpose of greater automatization in establishing aseptic in vitro tissue culture was conducted on cherry rootstocks CAB 6P and Gisela 6. Two ways of sterilization were examined (NaOCl and ozone O3) through survival rate on three types of explants; buds with primordial leaves, buds without primordial leaves and nodal non lignified segment with axillary buds. Ozone resulted with the high rate of survival (from 57 to 93%) regardless of the type of explants and the rootstock variety. By introducing a complete bud without destruction significantly resulted with the higher rate of survival (from 90 to 97%). Results indicate the potential for ozone sterilization of the starting material not only as an ostensible but the perforated (sub-cuticular transpiration) agent.Istraživanja s ciljem pronalaženja učinkovitih sterilizanata, u svrhu veće automatizacije u uspostavi aseptične kulture tkiva in vitro, provedena su na podlogama trešnje CAB 6P i Gisela 6. Ispitivana su dva načina sterilizacije (NaOCl i ozon O3), prateći stopu preživljavanja na tri tipa eksplantata; pupovi s primordijalnim lišćem, pupovi bez primordijalnoga lišća i nodijalni nelignificirani segment s aksilarnim pupom. Ozon je rezultirao visokom stopom preživljavanja (od 57 do 93%), bez obzira na vrstu eksplantata i varijante podloge. Uvođenjem cjelovitoga pupa bez destrukcije značajno je rezultiralo višom stopom preživljavanja (od 90 do 97%). Rezultati ukazuju na potencijal sterilizacije ozonom inicijalnoga biljnoga materijala, ne samo kao površinskoga, nego i penetrirajućeg (subkutikularnog) agenta

Impact of the fermentation process with immobilized yeast cells on the aroma profile and sensory quality of distillates produced from carob pods (Ceratonia siliqua L.)

The aim of this research was to investigate the influence of immobilized cell fermentation on aroma and sensory characteristics of distillates produced from carob pods (Ceratonia siliqua L.) commonly grown in coastal Croatia. Distillate samples were produced both by classical and immobilized yeast technology. The aroma profile was determined using GC/FID and a sensory analysis was conducted according to the German DLG model. Results showed that a immobilized cell technique gave distillates with lower ester contents, nevertheless satisfying sensory quality

Učinci ranog uklanjanja listova na koncentracije hlapljivih spojeva u Cabernet Sauvignon vinima iz vinogorja Ilok

The aim of this two year study was to evaluate effects of basal leaf removal before blooming on volatile composition of Cabernet Sauvignon wines in the Ilok vineyards (eastern Croatia). During two consecutive vintages (2013, 2014), two different treatments of basal leaf removal were applied: removal of 3 and 6 leaves, and control without leaf removal. Volatile compounds of resulting wines were identified and quantified by gas chromatography coupled with mass spectrometry. Organic acids and higher alcohols remained unaffected by treatments of early defoliation wine, except acetic acid and 2-methyl-6-hepten-1-ol. Early leaf removal was affected on six esters concentration only in the 2014 with different outcome. For compounds unaffected by defoliation, vintage was statistically significant source of variability, as the results of multivariate analysis have confirmed. Defoliation treatments in Cabernet Sauvignon wines from eastern continental part of Croatia should be adjusted to the weather condition in growing season.Cilj ovoga dvogodišnjeg istraživanja bio je procijeniti učinke uklanjanja bazalnoga lista prije cvatnje na sadržaj hlapljivih spojeva Cabernet Sauvignon vina u vinogradima Ilok (istočna Hrvatska). Tijekom dviju uzastopnih godina (2013. i 2014.) primijenjena su dva različita tretmana uklanjanja bazalnih listova: uklanjanje 3 lista, 6 listova te kontrola bez uklanjanja listova. Hlapljivi spojevi dobivenih vina identificirani su i kvantificirani spregnutom tehnikom plinske kromatografije i spektrometrije masa. Tretmani rane defolijacije nisu imali učinka na koncentraciju organskih kiselina i viših alkohola, osim na octenu kiselinu i 2-metil-6-hepten-1-ol. Rana defolijacija utjecala je koncentracije šest estera samo u vinima iz 2014. godine s različitim ishodom. Za spojeve na koje rana defolijacija nije imala učinka, berba je bila statistički značajan izvor varijabilnosti, kao što su rezultati multivarijantne analize potvrdili. Tretmane rane defolijacije u Cabernet Sauvignon vinima u istočnome kontinentalnome dijelu Hrvatske potrebno je prilagoditi vremenskim prilikama tijekom vegetacijske sezone

THE INFLUENCE OF MYCORRIZAL FUNGI INOCULATION ON THE GRAŠEVINA (Vitis vinifera L.) GRAPEVINE CULTIVAR’S PLANT NUTRIENT SUPPLY

Cilj istraživanja bio je utvrditi učinak živoga mikoriznog cjepiva na dinamiku opskrbljenosti biljnim hranivima vinove loze. Pokus je proveden na kultivaru Graševina (Vitis vinifera L.), cijepljenome na podlozi SO4 u uvjetima Vinogorja Kutjevo. Uzgojni oblik je Guyot, s opterećenjem od 10 pupova. U neposrednu blizinu korijenova sustava inokulirano je 20 ml mikorizne suspenzije koja sadrži oko 2000 propagacijskih jedinica živoga mikoriznog micelija. Istraživanje je provedeno u dvama klimatski vrlo različitim godinama. Prvu godinu istraživanja (2014.) karakterizirale su obilne oborine i manje sunčanih dana, što je rezultiralo kasnijom tehnološkom zrelošću grožđa od 34 dana u odnosu na 2015. godinu, koja je u periodu dozrijevanja grožđa bila iznadprosječno topla i suha. U obje godine istraživanja, u uvjetima slabe opskrbljenosti tla organskom tvari, fosforom i kalijem, mikorizna simbioza utjecala je na veći sadržaj fosfora i kalija u suhoj tvari list kultivara Graševine. U godini s više oborina i manje topline utvrđen je veći sadržaj dušika u listovima trsova s mikoriznom simbiozom. U suhim i toplim uvjetima tijekom cijeloga perioda dozrijevanja grožđa povećan je sadržaj kalcija, a opravdano veći sadržaj cinka, mangana i željeza utvrđen je u periodu neposredno prije tehnološke zrelosti grožđa. Nije utvrđen pozitivan utjecaj mikorize na urod, sadržaj šećera i ukupnu kiselost.The aim of the study was to determine the effect of a live mycorrhizal vaccine on the dynamics of grapevine’s plant nutrient supply. The experiment was performed on the Graševina variety (Vitis vinifera L.), grafted on an SO4 rootstock in the conditions of the Kutjevo Vineyards. The training system was Guyot, with a 10‐bud load. In the root system’s immediate vicinity, 20 ml of mycorrhizal suspension containing approximately 2,000 propagation units of a live mycorrhizal mycelium were inoculated. From the viticultural aspect, the research was conducted in two climatically very different years. The first research year (2014) was characterized by an abundant precipitation and less sunny days, resulting in a later technological grape maturity, amounting to 34 days when compared to the year 2015, which was extraordinarily warm and arid during the grape ripening period. In both research years, in the conditions of the soil’s low supply with the organic matter, phosphorus, and potassium, a mycorrhizal symbiosis influenced the higher content of phosphorus and potassium in the Graševina’s dry leaf matter. In a year experiencing more precipitation and less heat, a higher nitrogen content was found in the vine leaves manifesting a mycorrhizal symbiosis. In the dry and warm conditions, the calcium content was increased during the entire period of grape ripening, and a justifiably higher content of zinc, manganese, and iron was found in the period immediately preceding a technological grape ripeness. A positive influence of mycorrhiza on the yield, sugar content, and total acidity was not determined

Suitability assesment for vineyards in Kaštela-Trogir area

Analizom terenskih i laboratorijskih istraživanja utvrđeni su određeni nedostaci koji se u tlu očituju kroz nisku opskrbljenost fosforom i kalijem, povećan pH, visok sadržaj karbonata. Uz navedene nedostatke utvrđene su i pozitivne karakteristike kao što su: prisutnost skeleta različitih frakcija koji pokriva površinu vinograda i doprinosi zadržavanju vlage u tlu te potpomaže ukupnu insolaciju koja ima utjecaj na količinu šećera u samom grožđu, postotak humusa koji je unutar optimalnih 1 - 3 %, te se kreće od 1,20 - 2,80 %, količina oborina od 687,00 -1 436,00 litara koja zadovoljava potrebe vinove loze. Problem je njen raspored tijekom godine, odnosno nedostatak prilikom ljetnih mjeseci koji se rješava navodnjavanjem. Unatoč utvrđenim nedostacima, način na koji je izvedena sama priprema sadnje vinograda predstavlja novi aspekt zbrinjavanja materijala iz raznih građevinskih radova te se tako oplemenjuju površine uz racionaano odvajanje i zbrinjavanje materijala. Takvim pristupom dobivaju se nove proizvodne površine koje po svom izvornom obliku to nisu bile. Svi parametri koji su utvrđeni pokazuju kako ovakav pristup prostornog uređenja zemljišta ima potencijal te bi se svakim daljnjim radom uz prethodno stečena iskustva mogao poboljšati i unaprijediti. Nekontroliranim i neplanskim nasipavanjem dolazi do trajne i nepovratne degradacije tla, te će ovakav pristup u budućnosti doći sve više do izražaja, kako bi se izbalansirala potreba zbrinjavanja materijala i budućeg korištenja nasutog zemljišta.The analysis of field and laboratory research shows some deficiencies in the soil that are manifested through a low supply of phosphorus and potassium, increased pH, high carbonate content. In addition to these deficiencies identified were also and positive characteristics such as the presence of the rock material different fraction which is distributed by the surface of the vineyard, and which contributes to the retention of moisture in the soil and promotes overall insulation that has an impact on the amount of sugar in the grapes, the percentage of humus, which is within the optimal 1 - 3 %, and ranges from 1.20 to 2.80 %, the amount of rainfall is 687 - 1436 l and meets the needs of the vitis vinifera, the problem is her schedule during the year or during the summer months, the lack of which can be offset by irrigation. Despite the identified shortfalls, the way that is carried preparing planting vineyards represents a new aspect of dealing with waste material from a variety of construction projects and thereby enrich the surface with a rational separation and disposal of materials. Such an approach gives the new production area, which was not exits in its original form. All parameters that are determined show that this approach to zoning of land has potential and in any further work with the learned lessons, could improve and enhance. Uncontrolled and unplanned filling leads to permanent and irreversible soil degradation, and this approach will in future come increasingly to the fore, to balance the needs of housing materials and future use of the buried soil

Influence of light and hormone concentration on in vitro growth of shallot (Allium ascalonicum L.)

Rod Allium L. obuhvaća oko 750 vrsta. Unutar roda Allium nalazi se vrsta Allium cepa var. Ascalonicum L. poznata kao i ljutika (shallot). Za navedenu vrstu koriste se sinonimi: Allium cepa var. aggregatum G.Don i Allium cepa var. Ascalonicum L. Ljutika ili luk kozjak, (Allium ascalonicum) potječe s područja današnje Palestine i raširena je gotovo po cijelom Orijentu i srednjoj Aziji. Od davnina se koristi u ishrani i u narodnoj medicini. Kao i ostale vrste roda Allium ekstrakt ove vrste ima jaku antioksidativnu aktivnost i antimikrobno djelovanje Dvogodišnja je biljaka, koja se samorazmnožavanjem može održati više godina na istom mjestu. Glavna razlika između luka i ljutike je ta što ljutika ima manje lukovice koje rastu na istoj stabljici te ih može biti od 3 do 20. Cilj istraživanja je bio utvrditi utjecaj osvjetljenja i različite koncentracije hormona na početni porast eksplantata ljutike in vitro. Za istraživanje su korištene dvije različite varijante hranjive podloge (2,0 mg/L BAP i 5 mg/L BAP) te dvije varijante osvjetljenja, bijelo svjetlo i plavo svjetlo. Nakon dva tjedna uzgoja u kulturi određeni su masa, duljina i multiplikacijski indeks. Analizom varijance utvrđen je statistički značajan utjecaj (p < 0,01) koncentracije hormona na rast ljutike u prvom ciklusu. Najveća prosječna duljina i masa ljutike u prvom ciklusu izmjerena je kod tretmana bijelim svjetlom uz koncentraciju hormona 5 mg/L BAP. Analizom varijance utvrđen je statistički značajan utjecaj (p < 0,01) koncentracije hormona na rast ljutike. u drugom ciklusu. Prosječni indeks multiplikacije ljutike iznosio je 3,4133.Genus Allium L. embraces about 750 species. Within the genus Allium there is the species Allium cepa var. Ascalonicum L. Known as a Shallot. The synonym used for this species is Allium cepa var. Aggregate G.Don and Allium cepa var. Ascalonicum L. Shallot(Allium ascalonicum L.) is originates from the territory of today's Palestine and is spread almost throughout the Orient and Central Asia. Since ancient times it has been used in nutrition and in folk medicine. Like other Allium species, extract of shallot has strong antioxidant activity and antimicrobial activity. It is a two year plant, which can be sustained several years in the same place. The main difference between arch and anger is that the angel has fewer bulbs growing on the same stem and can range from 3 to 20.The aim of this study was to determine the influence of illumination and different hormone concentrations on the initial growth of in vitro excitement of the sting. Two different nutrient media variants (2.0 mg / L BAP and 5 mg / L BAP) were used for the study, and two variants of illumination, white light and blue light. After two weeks of cultivation, weight, length and multiplication index were determined. The variance analysis showed a statistically significant effect (p <0.01) of the hormone concentration on the incidence of sputum in the first cycle. The highest mean length and mass of first-cycle heart rate was measured in white light with a 5 mg/L BAP hormone concentration. The variance analysis showed a statistically significant effect (p <0.01) of the hormone concentration on the incidence of sputum. In the second cycle. The average multiplication index was 3,4133. Thesis performed at: Faculty of Agricultur

The Effectivness of Various Protection Means in Suppression Botrytis cinerea Pers. and Their Influence on Grape Yield and Must Quality cv. Cabernet Sauvignon ( Vitis vinifera L.)

Cilj ovog istraživanja bio je ispitati učinkovitost različitih oblika zaštite na pojavu i intenzitet zaraze s B. cinerea na kultivaru Cabernet sauvignon u vinogorju Đakovo te utvrditi razlike u kvalitativnim i kvantitativnim karakteristikama grožđa koje se javljaju pri primjeni različitih oblika zaštite s obzirom na zarazu uzrokovanu sivom plijesni. Istraživanje je provedeno u periodu od tri godine na Vinogradarsko-vinarskom pokušalištu Fakulteta agrobiotehničkih znanosti Osijek. Od kemijskih sredstava korišteni su Teldor SC 500 i Switch 62,5 WG, od bioloških preparata korišteni su preparati Trichodex WP i Serenade. Agro-ampelotehničke mjere obuhvaćale su: uklanjanje bazalnih listova i zaperaka, propuhivanje grozdova i primjenu kalcijevog klorida. Prosječan urod po trsu u 2015. godini iznosio je 1,18 kg, u 2016. godini 5,32 kg, a 2017. godini 3,18 kg. Prosječan sadržaj šećera u moštu bio je od 87,96 °Oe (2016.) do 97 °Oe (2017.). Ukupna kiselost mošta kretala se od 5,29 g/L (2015.) do 11,13 g/L (2016.). Prosječna vrijednost pH mošta bila je u rasponu od 3,18 (2016.) do 3,55 (2015.). Sadržaj ukupnih polifenola bio je od 442,42 mg/L (2015.) do 848,05 mg/L (2017.). Najmanji sadržaj ukupnih antocijana iznosio je 127,60 mg/L (2015.), a najveći 328,14 mg/L (2017.). Najmanja antioksidacijska aktivnost utvrđena je u 2015. godini (7,90 mg GAE/100 g), a najveća u 2017. godini (28,45 mg GAE/100 g). Najmanji postotak polimerne boje iznosio je 5,03 % (2017.), a najveći 21,28 % (2015.). Ocjena bolesti odrađena je prema EPPO skali (2001.) te je prosječna ocjena u 2015. godini iznosila 3,96. U 2016. godini utvrđena je sporadična prisutnost bolesti (1,25) na jednom tretmanu (konvencionalna zaštita botriticidima), dok u 2017. godini kod svih tretmana prisutnost bolesti nije zabilježena. Analizom varijance nije utvrđen statistički značajan utjecaj tretmana na ispitivane parametre niti je interakcija tretmana i godine bila značajna, osim u slučaju razlika u urodu kod tretmana skup ampelotehničkih mjera i primjena kalcijevog klorida za 2016. i 2017. godinu. Utvrđen je statistički značajan utjecaj godine na sve ispitivane parametreintensity of B. cinerea infection on the cultivar Cabernet Sauvignon in the Đakovo vineyards and to determine the differences in qualitative and quantitative parameters that occur when applying different forms of protection with regard to infection caused by gray mold. The research was conducted for a period of three years at a Viticulture and winemaking experimental station Faculty of Agrobiotechnical Sciences Osijek. Fungicides that were used were Teldor SC 500 and Switch 62.5 WG, and biofungicides Trichodex WP and Serenade. Ampelotechnical measures include: removal of basal leaves and cotyledons, blowing of clusters and application of Calcium Chloride. The average value of yield per vine in 2015 was 1.18 kg, in 2016 5.32 kg, and in 2017 3.18 kg. The average sugar content in the must ranged from 87.96 °Oe (2016) to 97 °Oe (2017). Total acidity was measured in the range of 5.29 g / L (2015) to the highest measured 11.13 g / L (2016). For the pH level, the average value ranged from 3.18 (2016) to 3.55 (2015). Total polyphenols ranged from 442.42 mg / L (2015) to 848.05 mg / L (2017). The lowest content of total anthocyanins was 127.60 mg / L (2015), and the highest was 328.14 mg / L (2017). In terms of antioxidant activity, the lowest average content of 7.90 mg / 100g (GAE) was determined in 2015. The highest average antioxidant activity was measured in 2017. (28.45 mg / 100g GAE). The highest average value of polymer color was 5.03% (2017), and the highest was 21.28% (2015). The assessment of the disease was coducted according to the EPPO scale (2001). Average presence of the disease in 2015 was 3.96. In 2016 presence of the disease (1.25) was determined in one treatment (conventional protection with botryticides), while in 2017, the presence of the disease was not recorded in all treatments. ANOVA (p <0.01) revealed a statistically significant difference between the treatments a set of ampelotechnical measures and calcium chloride (for 2016 and 2017), while between other treatments there was no significant difference in grape yield or interaction between treatments and year. A statistically significant influence of the year on all examined treatments was determine