Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry

This protocol describes the use of solid-phase cytometry for the enumeration of airborne bacteria and fungi. In contrast with conventional methods, accurate results can be obtained in real time, especially for air samples with low numbers of microorganisms. Air samples are collected by impaction on a water-soluble polymer that is subsequently dissolved. Part of the sample can be filtered over two membrane filters with different pore sizes. One filter is used to obtain a total count of all viable microorganisms, and a second filter is used to determine the number of airborne fungi. Microorganisms present on the filter are labeled with a viability substrate and subsequently detected and quantified using a solid-phase cytometer. The detected spots are microscopically validated using an epifluorescence microscope to discriminate between bacteria, fungi and fluorescent particles. The whole procedure takes 5 h to complete and results in the accurate quantification of airborne bacteria and fungi for samples with a low or high microbial load

Model Transformations with Tom

International audienceModel Driven Engineering (MDE) advocates the use of Model Transformations (MT) in order to automate repetitive development tasks. Many different model transformation languages have been proposed with a significant tool development cost as common language elements like expressions, statements, ... must be built from scratch for each new language development tools. The Tom language is a shallow extension of Java tailored to describe and implement transformations of tree based data-structures. A key feature of Tom allows to map any Java data-structure to tree based data abstractions that can then be accessed by powerful non-linear, associative, commutative pattern matching. In this paper, we present how this approach can be used in order to develop model transformations, in particular relying on Eclipse Modeling Framework (EMF) based metamodeling facilities. This allows to provide a transformation language at a low cost both for the development of its tools and the training of its users

The public domain vs. the museum: the limits of copyright and reproductions of two-dimensional works of art

The problem of museums and public institutions handling reproductions of works in their collections is not only a legal question but also one of museum ethics. Public museums are committed to spreading knowledge and to making their collections accessible. When it comes to images of their holdings, however, they often follow a restrictive policy. Even for works in the public domain they claim copyright for their reproductive photographs. This paper offers an analysis of the different interests at stake, a short survey of important cases, and practical recommendations

Nest predation in Afrotropical forest fragments shaped by inverse edge effects, timing of nest initiation and vegetation structure

High levels of nest predation influence the population dynamics of many tropical birds, especially when deforestation alters nest predator communities. The consequences of tropical forest fragmentation on nest predation, however, remain poorly understood, as natural predation patterns have only been well documented in a handful of tropical forests. Here, we show the results of an extensive study of predation on natural nests of Cabanis's Greenbul (Phyllastrephus cabanisi) during 3 years in a highly fragmented cloud forest in SE Kenya. Overall predation rates derived from 228 scrub nests averaged 69 %, matching the typical high predation level on tropical bird species. However, predation rates strongly varied in space and time, and a model that combined timing effects of fragment, edge, concealment, year and nest was best supported by our data. Nest predation rates consistently increased from forest edge to interior, opposing the classic edge effect on nest predation, and supporting the idea that classic edge effects are much rarer in Afrotropical forests than elsewhere. Nest concealment also affected predation rates, but the strength and direction of the relationship varied across breeding seasons and fragments. Apart from spatial variation, predation rates declined during the breeding season, although the strength of this pattern varied among breeding seasons. Complex and variable relationships with nest predation, such as those demonstrated here, suggest that several underlying mechanisms interact and imply that fixed nesting strategies may have variable-even opposing-fitness effects between years, sites and habitats

Polymorphic systems with arrays : decidability and undecidability

Polymorphic systems with arrays (PSAs) is a general class of nondeterministic reactive systems. A PSA is polymorphic in the sense that it depends on a signature, which consists of a number of type variables, and a number of symbols whose types can be built from the type variables. Some of the state variables of a PSA can be arrays, which are functions from one type to another. We present several new decidability and undecidability results for parameterised control-state reachability problems on subclasses of PSAs

Differences in life-histories refute ecological equivalence of cryptic species and provide clues to the origin of bathyal Halomonhystera (Nematoda)

The discovery of morphologically very similar but genetically distinct species complicates a proper understanding of the link between biodiversity and ecosystem functioning. Cryptic species have been frequently observed to co-occur and are thus expected to be ecological equivalent. The marine nematode Halomonhystera disjuncta contains five cryptic species (GD1-5) that co-occur in the Westerschelde estuary. In this study, we investigated the effect of three abiotic factors (salinity, temperature and sulphide) on life-history traits of three cryptic H. disjuncta species (GD1-3). Our results show that temperature had the most profound influence on all life-cycle parameters compared to a smaller effect of salinity. Life-history traits of closely related cryptic species were differentially affected by temperature, salinity and presence of sulphides which shows that cryptic H. disjuncta species are not ecologically equivalent. Our results further revealed that GD1 had the highest tolerance to a combination of sulphides, high salinities and low temperatures. The close phylogenetic position of GD1 to Halomonhystera hermesi, the dominant species in sulphidic sediments of the Hakon Mosby mud volcano (Barent Sea, 1280 m depth), indicates that both species share a recent common ancestor. Differential life-history responses to environmental changes among cryptic species may have crucial consequences for our perception on ecosystem functioning and coexistence of cryptic species

Controlling delay differentiation with priority jumps: analytical study

Supporting different services with different Quality of Service (QoS) requirements is not an easy task in modern telecommunication systems: an efficient priority scheduling discipline is of great importance. Fixed or static priority achieves maximal delay differentiation between different types of traffic, but may have a too severe impact on the performance of lower-priority traffic. In this paper, we propose a priority scheduling discipline with priority jumps to control the delay differentiation. In this scheduling discipline, packets can be promoted to a higher priority level in the course of time. We use probability generating functions to study the queueing system analytically. Some interesting mathematical challenges thereby arise. With some numerical examples, we finally show the impact of the priority jumps and of the system parameters