A Refined Approach to Model Anisotropy in the Lowermost Mantle

© 2018 Institute of Physics Publishing. All rights reserved. Seismic anisotropy in the lowermost mantle has been attributed to texture development during mantle convection. This study models texture evolution in a subducting slab impinging on the core-mantle boundary. Using a 3-dimensional geodynamic model with tracers recording the deformation history, a visco-plastic self-consistent (VPSC) model for polycrystal deformation, and relying on experimentally determined slip systems, aggregate grains with volume fractions of 60% orthorhombic silicate perovskite (MgSiO3), 20% cubic calcium perovskite (CaSiO3), and 20% cubic ferropericlase ((Mg,Fe)O) were deformed plastically and developed crystal preferred orientation. Forward and reverse perovskite (Pv)-postperovskite (PPv) phase transitions were included by allowing for likely orientation variant selections. Grain orientations, P (compression) and S (shear) wave velocity pole figures were calculated for each phase as well as the aggregate. The results show that dominant (001) slip on PPv can produce strong texture and shear wave anisotropy of 0.01-3.07% with VSH >VSV which agrees with seismological observations in selected areas of the D" layer

FADS2 Function Loss at the Cancer Hotspot 11q13 Locus Diverts Lipid Signaling Precursor Synthesis to Unusual Eicosanoid Fatty Acids

Background: Genes coding for the fatty acid desaturases (FADS1, 2, 3) localized at the cancer genomic hotspot 11q13 locus are required for the biosynthesis of 20 carbon polyunsaturated fatty acids (PUFA) that are direct eicosanoid precursors. In several cancer cell lines, FADS2 encoded D6 and D8 desaturation is not functional. Methodology/Principal Findings: Analyzing MCF7 cell fatty acids with detailed structural mass spectrometry, we show that in the absence of FADS2 activity, the FADS1 product D5-desaturase operates to produce 5,11,14–20:3 and 5,11,14,17–20:4. These PUFA are missing the 8–9 double bond of the eicosanoid signaling precursors arachidonic acid (5,8,11,14–20:4) and eicosapentaenoic acid (5,8,11,14,17–20:5). Heterologous expression of FADS2 restores D6 and D8-desaturase activity and normal eicosanoid precursor synthesis. Conclusions/Significance: The loss of FADS2-encoded activities in cancer cells shuts down normal PUFA biosynthesis, deleting the endogenous supply of eicosanoid and downstream docosanoid precursors, and replacing them with unusual butylene-interrupted fatty acids. If recapitulated in vivo, the normal eicosanoid and docosanoid cell signaling milieu would be depleted and altered due to reduction and substitution of normal substrates with unusual substrates, with unpredictable consequences for cellular communication

Influence of the Stability of a Fused Protein and Its Distance to the Amyloidogenic Segment on Fibril Formation

Conversion of native proteins into amyloid fibrils is irreversible and therefore it is difficult to study the interdependence of conformational stability and fibrillation by thermodynamic analyses. Here we approached this problem by fusing amyloidogenic poly-alanine segments derived from the N-terminal domain of the nuclear poly (A) binding protein PABPN1 with a well studied, reversibly unfolding protein, CspB from Bacillus subtilis. Earlier studies had indicated that CspB could maintain its folded structure in fibrils, when it was separated from the amyloidogenic segment by a long linker. When CspB is directly fused with the amyloidogenic segment, it unfolds because its N-terminal chain region becomes integrated into the fibrillar core, as shown by protease mapping experiments. Spacers of either 3 or 16 residues between CspB and the amyloidogenic segment were not sufficient to prevent this loss of CspB structure. Since the low thermodynamic stability of CspB (ΔGD = 12.4 kJ/mol) might be responsible for unfolding and integration of CspB into fibrils, fusions with a CspB mutant with enhanced thermodynamic stability (ΔGD = 26.9 kJ/mol) were studied. This strongly stabilized CspB remained folded and prevented fibril formation in all fusions. Our data show that the conformational stability of a linked, independently structured protein domain can control fibril formation

2 nd Brazilian Consensus on Chagas Disease, 2015

Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research

The influence of grain shape and volume fraction of sheet silicates on elastic properties of aggregates: Biotite platelets in an isotropic matrix

Elastic anisotropy of sheet-silicate-rich rocks such as shalesand slates strongly depends on the orientation distribution ofplatelet-shaped minerals, as well as shape and orientation ofpores. Bulk elastic anisotropy of the rock results in the anisotropywith respect to the propagation of elastic waves, and consequently,the fastest P-waves can travel with velocities exceedingthe slowest velocities by a factor of two or even greater. An importantfactor is the sheet-silicate's grain shapes.We approacheda model system of biotite platelets in an isotropic matrix withdifferent methods: A mean-field self-consistent method thatconsidered ellipsoidal particles in an effective anisotropic matrix,and a full-field method based on fast Fourier transformsthat considered the microstructure, the topology of the polycrystal,and local interactions. Both methods provided numericallyvery close results. Using these results, we predicted that the aggregatewith more oblate grain shape (thinner platelets) waselastically more anisotropic than the material with grains of lessoblate shape, but only for small volume fractions of orientedplatelets. For large fractions of platelets, the opposite was true.This switchover in the elastic anisotropy depended on texturestrength, platelet shape, and elastic properties of the isotropicmatrix

The influence of grain shape and volume fraction of sheet silicates on elastic properties of aggregates: Biotite platelets in an isotropic matrix

Elastic anisotropy of sheet-silicate-rich rocks such as shalesand slates strongly depends on the orientation distribution ofplatelet-shaped minerals, as well as shape and orientation ofpores. Bulk elastic anisotropy of the rock results in the anisotropywith respect to the propagation of elastic waves, and consequently,the fastest P-waves can travel with velocities exceedingthe slowest velocities by a factor of two or even greater. An importantfactor is the sheet-silicate's grain shapes.We approacheda model system of biotite platelets in an isotropic matrix withdifferent methods: A mean-field self-consistent method thatconsidered ellipsoidal particles in an effective anisotropic matrix,and a full-field method based on fast Fourier transformsthat considered the microstructure, the topology of the polycrystal,and local interactions. Both methods provided numericallyvery close results. Using these results, we predicted that the aggregatewith more oblate grain shape (thinner platelets) waselastically more anisotropic than the material with grains of lessoblate shape, but only for small volume fractions of orientedplatelets. For large fractions of platelets, the opposite was true.This switchover in the elastic anisotropy depended on texturestrength, platelet shape, and elastic properties of the isotropicmatrix

A double inclusion homogenization scheme for polycrystals with hierarchal topologies: Application to twinning in Mg alloys

The present work introduces a double inclusion elasto-plastic self-consistent (DI-EPSC) scheme for topologies in which crystals can contain subdomains (i.e. twins, etc.). The approach yields a direct coupling between the mechanical response of grains and their subdomains via a concentration relationship on mean fields derived from both the Eshelby and the Tanaka-Mori properties. The latent effect caused by twinning on the mechanical response is observed on both initially extruded and non-textured Mg alloys. For twinned grains, it is shown that deformation system activities and plastic strain distributions within twins drastically depend on the interaction with parent domains. Moreover, a quantitative study on the coupled influence of secondary slip activities on the material response is proposed. © 2014 Published by Elsevier Ltd