Numerical Computing and Graphics for the Power Method Transformation Using Mathematica

This paper provides the requisite information and description of software that perform numerical computations and graphics for the power method polynomial transformation. The software developed is written in the Mathematica 5.2 package PowerMethod.m and is associated with fifth-order polynomials that are used for simulating univariate and multivariate non-normal distributions. The package is flexible enough to allow a user the choice to model theoretical pdfs, empirical data, or a user's own selected distribution(s). The primary functions perform the following (a) compute standardized cumulants and polynomial coefficients, (b) ensure that polynomial transformations yield valid pdfs, and (c) graph power method pdfs and cdfs. Other functions compute cumulative probabilities, modes, trimmed means, intermediate correlations, or perform the graphics associated with fitting power method pdfs to either empirical or theoretical distributions. Numerical examples and Monte Carlo results are provided to demonstrate and validate the use of the software package. The notebook Demo.nb is also provided as a guide for user of the power method.

Numerical Computing and Graphics for the Power Method Transformation Using Mathematica

This paper provides the requisite information and description of software that perform numerical computations and graphics for the power method polynomial transformation. The software developed is written in the Mathematica 5.2 package PowerMethod.m and is associated with fifth-order polynomials that are used for simulating univariate and multivariate non-normal distributions. The package is flexible enough to allow a user the choice to model theoretical pdfs, empirical data, or a user’s own selected distribution(s). The primary functions perform the following (a) compute standardized cumulants and polynomial coefficients, (b) ensure that polynomial transformations yield valid pdfs, and (c) graph power method pdfs and cdfs. Other functions compute cumulative probabilities, modes, trimmed means, intermediate correlations, or perform the graphics associated with fitting power method pdfs to either empirical or theoretical distributions. Numerical examples and Monte Carlo results are provided to demonstrate and validate the use of the software package. The notebook Demo.nb is also provided as a guide for user of the power method

Über Pankreaszyste

Wir haben in unserer Klinik 3 Fälle von Pankreaszyste beobachtet. Sie waren alle Pseudzyste und haben sich als Species gastrocolica entwickelt uud wurden nach Gussenbauer operiert und geheilt entlassen. I-Fall: Ein 8 jähriges Mädchen bekam einen faustgrossen Tumor einen Monat nach Bauchtrauma. Es wurde in 33 Tagen nach der Operation geheilt entlassen. Der Zysteninhalt war klar und gelbbräunlich. In der Zystenflüssigkeit wurden Lipase und Diastase nachgewiesen. II-Fall: Ein 46 jähriger Mann konnte nicht veranlassendes Moment angeben. Es wurde zuerst als Magenbeschwerde und dann als Hydronephrose diagnostiert. Er wurde in 39 Tagen nach der Operation geheilt entlassen. In der klaren gelbbräunlichen Zystenflüssigkeit wurden Lipase und Tripsin nachgewiesen. III-Fall: Ein 38 jahrige Frau bemerkte einen kindskopfgrossen Tumor nach der Entbindung Die Zystenwand wurde operativ exzidiert und ergab sich histologisch als Pseudozyste. In 93 Tagen nach der Operation wurde sie geheilt entlassen. In der gelbbraunlichen Flüssigkeit des Inhaltes wurden Trypsin, Lipase und Diastase nachgewiesen

Assessing Symbiodinium diversity in scleractinian corals via next-generation sequencing-based genotyping of the ITS2 rDNA region.

The persistence of coral reef ecosystems relies on the symbiotic relationship between scleractinian corals and intracellular, photosynthetic dinoflagellates in the genus Symbiodinium. Genetic evidence indicates that these symbionts are biologically diverse and exhibit discrete patterns of environmental and host distribution. This makes the assessment of Symbiodinium diversity critical to understanding the symbiosis ecology of corals. Here, we applied pyrosequencing to the elucidation of Symbiodinium diversity via analysis of the internal transcribed spacer 2 (ITS2) region, a multicopy genetic marker commonly used to analyse Symbiodinium diversity. Replicated data generated from isoclonal Symbiodinium cultures showed that all genomes contained numerous, yet mostly rare, ITS2 sequence variants. Pyrosequencing data were consistent with more traditional denaturing gradient gel electrophoresis (DGGE) approaches to the screening of ITS2 PCR amplifications, where the most common sequences appeared as the most intense bands. Further, we developed an operational taxonomic unit (OTU)-based pipeline for Symbiodinium ITS2 diversity typing to provisionally resolve ecologically discrete entities from intragenomic variation. A genetic distance cut-off of 0.03 collapsed intragenomic ITS2 variants of isoclonal cultures into single OTUs. When applied to the analysis of field-collected coral samples, our analyses confirm that much of the commonly observed Symbiodinium ITS2 diversity can be attributed to intragenomic variation. We conclude that by analysing Symbiodinium populations in an OTU-based framework, we can improve objectivity, comparability and simplicity when assessing ITS2 diversity in field-based studies.We would like to thank the KAUST BioScience Core Lab and S. Neelamegam for 454 library generation and sequencing. We would also like to thank Y. Sawall and A. Al-Sofyani for provision and collection of coral samples, and three anonymous reviewers for helpful comments. This project was funded by a KAUST Academic Excellence Alliance (AEA) Award to CRV and CJH, baseline research funds to CRV and a National Science Foundation grant to TCL (OCE-09287664).This is the final published version. It first appeared at http://onlinelibrary.wiley.com/doi/10.1111/mec.12869/abstract

CD4 intragenic SNPs associate with HIV-2 plasma viral load and CD4 count in a community-based study from Guinea-Bissau, West Africa.

OBJECTIVES: The human genetics of HIV-2 infection and disease progression is understudied. Therefore, we studied the effect of variation in 2 genes that encode products critical to HIV pathogenesis and disease progression: CD4 and CD209. DESIGN: This cross-sectional study consisted of 143 HIV-2, 30 HIV-1 + HIV-2 and 29 HIV-1-infected subjects and 194 uninfected controls recruited from rural Guinea-Bissau. METHODS: We genotyped 14 CD4 and 4 CD209 single nucleotide polymorphisms (SNPs) that were tested for association with HIV infection, HIV-2 plasma viral load (high vs. low), and CD4 T-cell count (high vs. low). RESULTS: The most significant association was between a CD4 haplotype rs11575097-rs10849523 and high viral load [odds ratio (OR): = 2.37, 95% confidence interval (CI): 1.35 to 4.19, P = 0.001, corrected for multiple testing], suggesting increased genetic susceptibility to HIV-2 disease progression for individuals carrying the high-risk haplotype. Significant associations were also observed at a CD4 SNP (rs2255301) with HIV-2 infection (OR: = 2.36, 95% CI: 1.19 to 4.65, P = 0.01) and any HIV infection (OR: = 2.50, 95% CI: 1.34 to 4.69, P = 0.004). CONCLUSIONS: Our results support a role of CD4 polymorphisms in HIV-2 infection, in agreement with recent data showing that CD4 gene variants increase risk to HIV-1 in Kenyan female sex workers. These findings indicate at least some commonality in HIV-1 and HIV-2 susceptibility

HSP90 Inhibitor, NVP-AUY922, Improves Myelination in Vitro and Supports the Maintenance of Myelinated Axons in Neuropathic Mice.

Hereditary demyelinating neuropathies linked to peripheral myelin protein 22 (PMP22) involve the disruption of normal protein trafficking and are therefore relevant targets for chaperone therapy. Using a small molecule HSP90 inhibitor, EC137, in cell culture models, we previously validated the chaperone pathway as a viable target for therapy development. Here, we tested five commercially available inhibitors of HSP90 and identified BIIB021 and AUY922 to support Schwann cell viability and enhance chaperone expression. AUY922 showed higher efficacy, compared to BIIB021, in enhancing myelin synthesis in dorsal root ganglion explant cultures from neuropathic mice. For in vivo testing, we randomly assigned 2-3 month old C22 and 6 week old Trembler J (TrJ) mice to receive two weekly injections of either vehicle or AUY922 (2 mg/kg). By the intraperitoneal (i.p.) route, the drug was well-tolerated by all mice over the 5 month long study, without influence on body weight or general grooming behavior. AUY922 improved the maintenance of myelinated nerves of both neuropathic models and attenuated the decline in rotarod performance and peak muscle force production in C22 mice. These studies highlight the significance of proteostasis in neuromuscular function and further validate the HSP90 pathway as a therapeutic target for hereditary neuropathies

Opportunities for CO2 Storage around Scotland; An Integrated Strategic Research Study

Carbon Capture and Storage (CCS) is one of the critical technologies worldwide which will enable reduction of carbon dioxide (CO2) emissions arising from large industrial sites. CCS allows the continued use of a diverse mix of energy sources, including fossil fuels, which improves the security of cost-effective electricity supply. Scotland has the opportunity and responsibility to reduce CO2 emissions arising from burning of fossil fuels and their impact on climate change. The EU plans to have 12 CCS plants operating by 2015. In February 2009, the UK Secretary of State for Energy and Climate Change stated an aspiration for the UK to have more than one demonstration project in operation enabled by government funding. However, these targets cannot be delivered without the underpinning knowledge from studies such as this. Commitment to large-scale investment in CO2 capture plant will require proven storage capability. This study • presents the first high-level screening of CO2 storage sites available to Scotland • evaluates the means by which CO2 can be transported from power plants and other industrial activities to storage sites, and • investigates the costs and business constraints. This is the most comprehensive and fully integrated study performed in the UK, and was achieved by a collaborative partnership of Scottish Government, research universities and institutes, and a broad base of support from industry and business. The conclusions show that Scotland has an extremely large CO2 storage resource. This is overwhelmingly in offshore saline aquifers (deeply buried porous sandstones filled with salt water) together with a few specific depleted hydrocarbon fields. The resource can easily accommodate the industrial CO2 emissions from Scotland for the next 200 years. There is very likely to be sufficient storage to allow import of CO2 from NE England, this equating to over 25% of future UK large industry and power CO2 output. Preliminary indications are that Scotland's offshore CO2 storage capacity is very important on a European scale, comparable with that of offshore Norway, and greater than Netherlands, Denmark and Germany combined.Carbon Capture and Storage (CCS) is one of the critical technologies worldwide which will enable reduction of carbon dioxide (CO2) emissions arising from large industrial sites. CCS allows the continued use of a diverse mix of energy sources, including fossil fuels, which improves the security of cost-effective electricity supply. Scotland has the opportunity and responsibility to reduce CO2 emissions arising from burning of fossil fuels and their impact on climate change. The EU plans to have 12 CCS plants operating by 2015. In February 2009, the UK Secretary of State for Energy and Climate Change stated an aspiration for the UK to have more than one demonstration project in operation enabled by government funding. However, these targets cannot be delivered without the underpinning knowledge from studies such as this. Commitment to large-scale investment in CO2 capture plant will require proven storage capability. This study • presents the first high-level screening of CO2 storage sites available to Scotland • evaluates the means by which CO2 can be transported from power plants and other industrial activities to storage sites, and • investigates the costs and business constraints. This is the most comprehensive and fully integrated study performed in the UK, and was achieved by a collaborative partnership of Scottish Government, research universities and institutes, and a broad base of support from industry and business. The conclusions show that Scotland has an extremely large CO2 storage resource. This is overwhelmingly in offshore saline aquifers (deeply buried porous sandstones filled with salt water) together with a few specific depleted hydrocarbon fields. The resource can easily accommodate the industrial CO2 emissions from Scotland for the next 200 years. There is very likely to be sufficient storage to allow import of CO2 from NE England, this equating to over 25% of future UK large industry and power CO2 output. Preliminary indications are that Scotland's offshore CO2 storage capacity is very important on a European scale, comparable with that of offshore Norway, and greater than Netherlands, Denmark and Germany combined

Motor cortical excitability and pre-supplementary motor area neurochemistry in healthy adults with substantia nigra hyperechogenicity

Substantia nigra (SN) hyperechogenicity, viewed with transcranial ultrasound, is a risk marker for Parkinson\u27s disease. We hypothesized that SN hyperechogenicity in healthy adults aged 50 – 70 years is associated with reduced short-interval intracortical inhibition in primary motor cortex, and that the reduced intracortical inhibition is associated with neurochemical markers of activity in the pre-supplementary motor area (pre-SMA). Short-interval intracortical inhibition and intracortical facilitation in primary motor cortex was assessed with paired-pulse transcranial magnetic stimulation in 23 healthy adults with normal (n = 14; 61 ± 7 yrs) or abnormally enlarged (hyperechogenic; n = 9; 60 ± 6 yrs) area of SN echogenicity. Thirteen of these participants (7 SN − and 6 SN+) also underwent brain magnetic resonance spectroscopy to investigate pre-SMA neurochemistry. There was no relationship between area of SN echogenicity and short-interval intracortical inhibition in the ipsilateral primary motor cortex. There was a significant positive relationship, however, between area of echogenicity in the right SN and the magnitude of intracortical facilitation in the right (ipsilateral) primary motor cortex (p = .005; multivariate regression), evidenced by the amplitude of the conditioned motor evoked potential (MEP) at the 10 – 12 ms interstimulus interval. This relationship was not present on the left side. Pre-SMA glutamate did not predict primary motor cortex inhibition or facilitation. The results suggest that SN hyperechogenicity in healthy older adults may be associated with changes in excitability of motor cortical circuitry. The results advance understanding of brain changes in healthy older adults at risk of Parkinson\u27s disease