Transmission structured illumination microscopy with tunable frequency illumination using tilt mirror assembly

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the illumination module where the sample is excited with the interference of two beams reflected from the opposite pair of mirror facets. Tunable frequency structured patterns are generated by changing the mirror-tilt angle and the hexagonal-symmetric arrangement is considered for the isotropic resolution in three orientations. Utilizing high numerical aperture (NA) objective in standard SIM provides super-resolution compromising with the field-of-view (FOV). Employing low NA (20X/0.4) objective lens detection, we experimentally demonstrate ∼ (0.56 mm× 0.35 mm) size single FOV image with ∼ 1.7- and ∼ 2.4-fold resolution improvement (exploiting various illumination by tuning tilt-mirrors) over the diffraction limit. The results are verified both for the fluorescent beads as well as biological samples. The tSIM geometry decouples the illumination and the collection light paths consequently enabling free change of the imaging objective lens without influencing the spatial frequency of the illumination pattern that are defined by the tilt-mirrors. The large and scalable FOV supported by tSIM will find usage for applications where scanning large areas are necessary as in pathology and applications where images must be correlated both in space and time

On Values

This thesis explores the relationship between human beings and values. Using the philosophy of Frierdrich Nietzsche as a context, the thesis accounts for the emergence of such values as good and evil as coming out of the long and sordid history of humanity. The thesis attempts to unravel the paradox of the value of these particular values. What do these values mean? What purpose do they serve? Do we as humans have any reason to accept or reject our values

Symposium: Kant and the Reorientation of Aesthetics (book précis and replies to critics)

Précis of _Kant and the Reorientation of Aesthetics_ and replies to comments by Samantha Matherne and Moran Godess-Riccitelli

Improving the space-bandwidth product of structured illumination microscopy using a transillumination configuration

Applying structured instead of plane illumination in widefield optical fluorescence microscopy can improve the spatial resolution beyond what is known as the Abbe limit. In general it is not only the resolution of an imaging system that is of interest but also its field of view (FOV). These two parameters are expressed in the space-bandwidth product (SBP). Here we introduce a modified structured illumination microscopy (SIM) approach that offers a larger SBP than any other available implementation. This is achieved through a transillumination geometry instead of the typical epifluorescent configuration. Compared to conventional SIM, the illumination path is decoupled from the objective lens by using a multi-mirror setup to generate the sinusoidal interference pattern for structured illumination in transmission mode. The spatial frequency of the illumination pattern can be controlled by changing the angle of the mirrors, achieving comparably fine patterns over a large FOV. In this work simulation results demonstrate the potential resolution improvement to be expected by the suggested implementation. Preliminary experimental results demonstrate phase-shifting ability and the stability of fringe frequencies over a large FOV of (≈16 mm2) at different numerical apertures, fulfilling the prerequisites for SIM acquisition

SJU Class of 2001 Commencement Celebration

May 27, 2001 One-Hundred Forty-Fourth Year Abbey & University Church Saint John\u27s University Fred de Sam Lazaro was the guest speaker and Joseph Tinguely was the student speaker

Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum

Recombinant P. somniferum salutaridine reductase (SalR) was purified and crystallized with NADPH using the hanging-drop vapor-diffusion method. Crystals of the SalR–NADPH complex diffracted X-rays to a resolution of 1.9 Å