It\u27s A Long, Long Way From Here To Mexico

Soldier wearing uniform and blowing horn; Tent, American flag, men on horses and cannons in valley, and mountains in backgroundhttps://scholarsjunction.msstate.edu/cht-sheet-music/11455/thumbnail.jp

BamView: visualizing and interpretation of next-generation sequencing read alignments.

So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes. BamView has been developed to visualize and analyse sequence reads from NGS platforms, which have been aligned to a reference sequence. It is a desktop application for browsing the aligned or mapped reads [Ruffalo, M, LaFramboise, T, Koyutürk, M. Comparative analysis of algorithms for next-generation sequencing read alignment. Bioinformatics 2011;27:2790-6] at different levels of magnification, from nucleotide level, where the base qualities can be seen, to genome or chromosome level where overall coverage is shown. To enable in-depth investigation of NGS data, various views are provided that can be configured to highlight interesting aspects of the data. Multiple read alignment files can be overlaid to compare results from different experiments, and filters can be applied to facilitate the interpretation of the aligned reads. As well as being a standalone application it can be used as an integrated part of the Artemis genome browser, BamView allows the user to study NGS data in the context of the sequence and annotation of the reference genome. Single nucleotide polymorphism (SNP) density and candidate SNP sites can be highlighted and investigated, and read-pair information can be used to discover large structural insertions and deletions. The application will also calculate simple analyses of the read mapping, including reporting the read counts and reads per kilobase per million mapped reads (RPKM) for genes selected by the user

Entendendo a escassez de professores: Uma análise da oferta e demanda de professores nos Estados Unidos

This paper reviews the sources of and potential solutions to teacher shortages in the United States. It describes the sources of current and projected increases in teacher demand relative to enrollments, shifts in pupil-teacher ratios, and attrition. It places these in relation to recent declines in teacher supply and evaluates evidence of shortages in fields like mathematics, science, special education, and educators for English learners, as well as in particular parts of the country. Our analysis using national databases through 2016 predicted an estimated annual teacher shortage of approximately 112,000 teachers in 2017-18. Our recent review of state teacher workforce reports estimated 109,000 individuals were uncertified for their teaching positions in the US in 2017, roughly approximating our projections. We discuss the factors driving shortages and, based on previous research, identify responses that might ameliorate these trends.Este artículo analiza las fuentes de escasez de maestros en los Estados Unidos y posibles soluciones para este situación. Describe las fuentes de aumentos actuales y proyectados en la demanda de maestros en relación a las matrículas, cambios en las proporciones alumno- maestro y la atrición. En relación con la disminución reciente en la oferta de maestros, encontramos evidencia de escasez en campos como matemáticas, ciencias, educación especial y educadores para estudiantes de inglés, así como en partes específicas del país. Nuestro análisis utilizando bases de datos nacionales hasta 2016 predijo una escasez anual estimada de aproximadamente 112.000 profesores en 2017-18. Nuestra reciente revisión de los informes de la fuerza de trabajo de maestros del estado estimó que 109.000 personas no fueron certificadas para sus posiciones de enseñanza en los EE.UU. En 2017, acercándose a nuestras proyecciones. Discutimos los factores que impulsan la escasez y, sobre la base de investigaciones anteriores, identificamos respuestas que pueden mejorar esas tendencias.Este artigo analisa as fontes de escassez de professores nos Estados Unidos e possíveis soluções para este dliema. Ele descreve as fontes de aumentos atuais e projetados na demanda de professores em relação às matrículas, mudanças nas proporções aluno-professor e atrito. Em relação aos declínios recentes na oferta de professores, encontramos evidências de escassez em áreas como matemática, ciências, educação especial e educadores para alunos de inglês, bem como em partes específicas do país.Nossa análise usando bases de dados nacionais até 2016 previu uma escassez anual estimada de aproximadamente 112.000 professores em 2017-18. Nossa recente revisão dos relatórios da força de trabalho de professores do estado estimou que 109.000 pessoas não foram certificadas para suas posições de ensino nos EUA. Em 2017, aproximando-se de nossas projeções. Discutimos os fatores que impulsionam a escassez e, com base em pesquisas anteriores, identificamos respostas que podem melhorar essas tendências

Evaluating the Ornamental Potentials of Four Native Texas Coastal Species

With increasing demand for high quality irrigation water and coastal development, new plants need to be developed that thrive with the use of saline irrigation and provide an alternative to invasive exotic landscape plants. Accessions of Erigeron procumbens (Houst. ex Mill.) G.L. Nesom, Borrichia frutescens (L.) DC., Sesuvium portulacastrum (L.) L., and Oenothera drummondii Hook. were collected along the Texas coast from Port Isabel to Port Arthur. Then taxa were screened for phenotypic variability, responses to plant growth regulators, and tolerances to saline irrigation water. Phenotypic variability appeared most promising in O. drummondii and B. frutescens for future breeding efforts. There were differences among accessions for all four species and there were regional differences in flowering and height for B. frutescens and O. drummondii. For B. frutescens height was reduced 54.9% by paclobutrazol at 40 mg a.i.∙pot^-1 and 34.9% by uniconazole at 2 mg a.i.∙pot^-1 when applied as a drench. Height and plant growth in O. drummondii was controlled with paclobutrazol drenches of 30 mg a.i.∙pot^-1 and uniconazole drenches of 1.5 mg a.i.∙pot^-1. Similar results were found in E. procumbens and S. portulacastrum, with spray applications of PGRs being generally ineffective on all four taxa. All four taxa were irrigated with four concentrations of saline water, with electrical conductivities (EC) of 0.8, 15.1, 23.8, 51.3, and 92.5 mS∙cm^-1, either applied sub-canopy or over the foliage. Concentrations above the control roughly represented the salinity of quarter, half, full, and double the salinity of seawater, respectively. All four species survived irrigation water with an EC of 23.8 mS∙cm^-1 and showed minimal damage with 15.1 mS∙cm^-1. Growth responses, mineral nutrient content, and K/Na ratios were consistent with reports of the halophytic nature of S. portulacastrum, which tolerated the greatest salinity exposure. Due to commercial interest, mating system and pollen storage studies were carried out on O. drummondii. In mating system experiments, out crossed flowers had a mean seed count of 240.5 (± 17.5) seed∙fruit^-1 and selfed flowers across all dates and treatments had a mean seed count of 285.0 (± 14.1) seeds∙fruit^-1 after 10 d on filter paper. This indicates a facultative out-crossing species and emasculation of flowers is not necessary in pollinator excluded environments

Exchange-coupling constants, spin density map, and Q dependence of the inelastic neutron scattering intensity in single-molecule magnets

The Q dependence of the inelastic neutron scattering (INS) intensity of transitions within the ground-state spin multiplet of single-molecule magnets (SMMs) is considered. For these transitions, the Q dependence is related to the spin density map in the ground state, which in turn is governed by the Heisenberg exchange interactions in the cluster. This provides the possibility to infer the exchange-coupling constants from the Q dependence of the INS transitions within the spin ground state. The potential of this strategy is explored for the M = +-10 -> +- 9 transition within the S = 10 multiplet of the molecule Mn12 as an example. The Q dependence is calculated for powder as well as single-crystal Mn12 samples for various exchange-coupling situations discussed in the literature. The results are compared to literature data on a powder sample of Mn12 and to measurements on an oriented array of about 500 single-crystals of Mn12. The calculated Q dependence exhibits significant variation with the exchange-coupling constants, in particular for a single-crystal sample, but the experimental findings did not permit an unambiguous determination. However, although challenging, suitable experiments are within the reach of today's instruments.Comment: 11 pages, 6 figures, REVTEX4, to appear in PR

Laboratory tests to understand tephra sliding behaviour on roofs

Following explosive eruptions, loading from tephra fall deposits can lead to roof collapse. However, the load may be reduced significantly by tephra sliding on pitched roofs. We present small-scale laboratory tests to investigate tephra sliding behaviour on metal, fibre cement sheet and tile roofing. We tested 10–30 cm thicknesses for dry and wet deposits of pumice, scoria and basaltic ash. We found that tephra did not slide on roof pitches ≤ 15° for coarse-grained deposits and ≤ 12° for dry ash. Thin deposits of wet ash were stable at pitches ≤ 30°. In addition, tephra was mainly shed on pitches ≥ 32° for metal roofs and ≥ 35° for fibre cement and tiles. Using these results, we have produced an initial set of sliding coefficients for tephra for simply pitched roofs that can be used to help prioritise roofs for clearing during an eruption and assist in designing roofs to withstand tephra fall

The Acidic Probe LysoSensor™ is not Useful for Acrosome Evaluation of Cryopreserved Ram Spermatozoa

P. 363-367To try new acrosomal probes for assessing ram spermatozoa, we compared the LysoSensor™ probe, which labels acidic organelles, with the frequently used peanut agglutinin acrosomal probe (PNA‐PE; phycoerythrin as fluorescent moiety). The previous microscopic observations showed a lack of relationship of LysoSensor™ with acrosomal status. Semen was obtained from five rams and frozen in four pools. Each pool was analysed carrying out a triple staining propidium ioide/PNA‐PE/LysoSensor™ Green DND‐189 to test acrosome labelling, and a double staining SYBR‐14/PI, to assess sperm viability. Stained samples were analysed by flow cytometry. All measurements were replicated. Data were processed using agreement and repeatability tests. LysoSensor™ labelling did not agree with PNA (mean of differences: 30.8%; coefficient of agreement: 22.6%), confirming microscopic observations. Nevertheless, when LysoSensor™ was compared with SYBR‐14/PI, the agreement was high (mean of differences: −0.05%; coefficient of agreement: 5.07%). Repeatability of both methods was high and similar. LysoSensor™ did not seem to specifically stain the acrosome, but it may accumulate in the cytoplasm and label viable spermatozoa. Therefore, LysoSensor™ might not be used as an acrosomal probe in ram spermatozoa, but it could be used in other kind of studies, taking advantage of its pH sensitivity.S