Artesunate ameliorates experimental autoimmune encephalomyelitis by inhibiting leukocyte migration to the central nervous system

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOExperimental autoimmune encephalomyelitis (EAE) is T-celldependent disease of the central nervous system (CNS) of mice. This model resembles multiple sclerosis (MS) in many aspects. Therapies that focus in the modulation of the immune response and cellular infiltration in the CNS present best effects in the clinics. Artesunate (Art) is a semi-synthetic sesquiterpene derivative from artemisinin and has been shown to reduce the clinical signs of autoimmune disease models through mechanisms not yet understood. In this study, we aimed to evaluate whether administration of Art would ameliorate EAE. Methods and Results: C57BL6 mice were immunized with MOG(35-55) peptide to induce EAE. At the same time, Art treatment started (3 mg/kg/day via i.p.) for five consecutive days. We found that Art treatment reduced the clinical signs of EAE and that correlated with a reduced infiltration of cells in the CNS. Disease amelioration did not correlate with immunomodulation as recall responses, leukocyte subpopulations, and gene expression analysis were similar among treated and untreated mice. Ultimately, further analysis provided data indicating that a possible mechanism of action for Art is dependent on the cellular migration to the CNS. Conclusions: Artesunate reduces the severity of EAE by inhibiting migration of pathogenic T cells to the CNS.Experimental autoimmune encephalomyelitis (EAE) is T-celldependent disease of the central nervous system (CNS) of mice. This model resembles multiple sclerosis (MS) in many aspects. Therapies that focus in the modulation of the immune response and cellula228707714FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO2014/19492-3; 2014/02631-0; 2014/11588-

Analysis of mechanical traction in biofilms chitosan and clay aiming fruits coating

A new concept of food packaging has been studied and developed in order to meet the application needs of the market. The application of such biofilms coating is directly related to its characteristics and possible interactions of these with the product and the environment. There is evidence that chitosan is a viable alternative to be applied in the coating for fruit characteristics antifungal, antibacterial and its direct action as a barrier to water vapor, which is the latest aging fruit. The clay, besides increasing permeability to water vapor, enables a greater adhesion of the film on the fruit surface. This paper presents the results that were obtained from the development of chitosan films aimed at applying the coating of fruit. It were made tensile test with films using different chitosan and clay concentrations. The tests followed the standards of ASTM D882/02. The best results were obtained for the coating of chitosan in 2% acetic acid, which showed a plastic strain of 4.4% and a maximum stress at rupture of 50.2 MPa. The results were lower for the films with the addition of clay; the best result was observed for this coating with chitosan to 2% clay plus 2% acetic acid, with plastic deformation of 2.9% and maximum stress at rupture 18.9 MPa

Differential brain and spinal cord cytokine and BDNF levels in experimental autoimmune encephalomyelitis are modulated by prior and regular exercise

The interactions between a prior program of regular exercise and the development of experimental autoimmune encephalomyelitis (EAE)-mediated responses were evaluated. in the exercised EAE mice, although there was no effect on infiltrated cells, the cytokine and derived neurotrophic factor (BDNF) levels were altered, and the clinical score was attenuated. Although, the cytokine levels were decreased in the brain and increased in the spinal cord, BDNF was elevated in both compartments with a tendency of lesser demyelization volume in the spinal cord of the exercised EAE group compared with the unexercised. (C) 2013 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)NIHUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Fisiol & Biofis, Nucleo Neurociencias, BR-31270901 Belo Horizonte, MG, BrazilMinist Educ Brazil, CAPES Fdn, Programa Pos Grad Ciencias Biol Fisiol Farmacol, BR-70040020 Brasilia, DF, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Patol, BR-31270901 Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Lab Venenos & Toxinas Anim, BR-31270901 Belo Horizonte, MG, BrazilUniv Miami, Miller Sch Med, Miami Project Cure Paralysis, Miami, FL 33136 USALa Trobe Univ, Dept Biochem, Bundoora, Vic 3086, AustraliaUniversidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, BrazilCAPES: BEX 0020/12-5NIH: NS051709NIH: NS065479FAPEMIG: CBB-APQ-01459-10FAPEMIG: PPM-00200-12Web of Scienc

Aspectos anatomopatológicos das neoplasias malignas renais: Anatomopathological aspects of malignant renal neoplasms

As neoplasias renais correspondem ao crescimento exacerbado de células tumorais no interior dos rins, classificadas como benignas ou malignas. Neste estudo será abordado sobre as neoplasias malignas renais, a qual correspondem a maior prevalência e são representadas pelo carcinoma de células renais e o tumor de Wilms, com a finalidade de descrever a respeito dos aspectos anatomopatológicos, disseminando informações para o diagnóstico e manejo precoce. O carcinoma de células renais é mais prevalente no sexo masculino, indivíduos mais velhos, geralmente assintomático, contribuindo para o diagnóstico tardio junto a existência de metástases e terapêutica irresponsiva. Não se trata de uma doença genética, sendo o caráter esporádico o predominante, neste contexto os fatores de risco, sobretudo o tabagismo em seguida de obesidade hemodiálise e doenças genéticas são potenciais desencadeantes da enfermidade. Os exames complementares associado a clínica, junto ao acompanhamento eleva a possibilidade de identificação antes de avanços metastáticos. O tumor de Wilms é típico de crianças, acometendo um ou ambos os rins, normalmente com alguma anomalia genética, sendo os sinais inespecíficos, mas sempre manifestando massa palpável e dor abdominal, a qual os métodos de imagem confirmam o diagnóstico e estimam o prognóstico deste. Neste contexto, elucida-se a transcendência que os aspectos anatomopatológicos das neoplasias malignas renais oferecem para a diagnose precoce, devido a escassez e inespecificidafe das manifestações clínicas. Logo, a junção do perfil de cada neoplasia abordado conduz ao manejo adequado e reduz a incidência de tratamentos agressivos e irresponsivos

Rendimento do milho em função dos modos de adubação em diferentes preparos de solo

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Rendimento do milho em função dos modos de adubação em diferentes preparos de solo

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Influência de três variedades de milho (zea mays, l.) E dois substratos na produção de forragem hidropônica

The present work shows the chemical and bromatological results of maize forage under the hydroponics method for animal feeding, cultivated in the section of University �Dr. Francisco Maeda� in Ituverava-SP, using seeds of three maize varieties without treatment produced by CATI, (AL - 30, AL -34 and AL - Bandeirante). Were tested with four repetitions in factorial plan, each treatment composed by a different variety, cultivated in two substratum type (sugarcane bagasse and rice hulls), in the sowing density (2Kg/m2) and with age of cut of 41 days. For the climate conditions studied, it was ended that the forage of hydroponics maize obtained by the three cultivate presented adequate levels of nutrients and good forage potential for use in the animal feeding, and the rice hulls produced better quality forage.O presente trabalho teve por objetivo avaliar a composição química e bromatológica da forragem de milho hidropônico para alimentação animal. A forragem foi cultivada no setor de olericultura da Faculdade �Dr. Francisco Maeda� no município de Ituverava-SP, utilizando sementes de três variedades de milho sem tratamento produzidas pela Coordenadoria de Assistência Técnica e Integral (CATI), (AL � 30, AL �34 XV e AL �Bandeirante). Foram testadas três variedades em dois substratos com quatro repetições aleatoriamente em esquema fatorial. Cada tratamento foi composto por uma variedade diferente, cultivadas em dois tipo de substrato (bagaço de cana e palha de arroz), na densidade de semeadura (2Kg/m2) e com idade de corte de 41 dias. Nas condições de clima estudadas, concluiu-se que a forragem de milho hidropônico obtida pelas três cultivares apresentou níveis considerados adequados de nutrientes e alto potencial de forragem para uso na alimentação animal, sendo que a palha de arroz produziu uma forragem de melhor qualidade