Analysis of pore-fluid pressure gradient and effective vertical-stress gradient distribution in layered hydrodynamic systems

A theoretical analysis is carried out to investigate the pore-fluid pressure gradient and effective vertical-stress gradient distribution in fluid saturated porous rock masses in layered hydrodynamic systems. Three important concepts, namely the critical porosity of a porous medium, the intrinsic Fore-fluid pressure and the intrinsic effective vertical stress of the solid matrix, are presented and discussed. Using some basic scientific principles, we derive analytical solutions and explore the conditions under which either the intrinsic pore-fluid pressure gradient or the intrinsic effective vertical-stress gradient can be maintained at the value of the lithostatic pressure gradient. Even though the intrinsic pore-fluid pressure gradient can be maintained at the value of the lithostatic pressure gradient in a single layer, it is impossible to maintain it at this value in all layers in a layered hydrodynamic system, unless all layers have the same permeability and porosity simultaneously. However, the intrinsic effective vertical-stress gradient of the solid matrix can be maintained at a value close to the lithostatic pressure gradient in all layers in any layered hydrodynamic system within the scope of this study

Investigation of biologically active zeolite: role of colonization in the removal of 14C-labelled sulfamethoxazole in wastewater

Up-to-date approaches to remove micropollutants in wastewater treatment are based on adsorbing materials like activated carbon. These fossil-based materials can also provide a surface for microbial colonization, which could further improve the removal of MPs. As zeolite filters have shown interesting performance in the removal of MPs in previous works, this study aimed to investigate the effect of microbial colonization on such filters on the elimination of 14C-labelled sulfamethoxazole (SMX), an antibiotic from the class of sulfonamides. Lab scale removal tests were set in 100 mL reactors and monitored for 150 days at room temperature. Taxa known to be linked to organic pollutant degradation (Caulobacterales, Rhizobiales, Burkholderiales) were found among the microbial community attached to the zeolite. Bacterial colonization of zeolite filters improved the removal of 14C-sulfamethoxazole by 35 % compared to the control. An analysis of the microbial community dynamics over time revealed the increased abundance of the Vicinamibacterales taxon after 50 days of contact with SMX. This order abundance, linked to degradation of sulfonamides, went from 0 to 17 %; and Shannon diversity ranged from 1.51 to 1.99. Data showed that zeolite filters as adsorbing material in wastewater treatment plants can improve MPs removal by supporting bacterial colonization, making it an interesting support that could synergize with biological activated carbon

The performance of stochastic designs in wellbore drilling operations

© 2018, The Author(s). Wellbore drilling operations frequently entail the combination of a wide range of variables. This is underpinned by the numerous factors that must be considered in order to ensure safety and productivity. The heterogeneity and sometimes unpredictable behaviour of underground systems increases the sensitivity of drilling activities. Quite often the operating parameters are set to certify effective and efficient working processes. However, failings in the management of drilling and operating conditions sometimes result in catastrophes such as well collapse or fluid loss. This study investigates the hypothesis that optimising drilling parameters, for instance mud pressure, is crucial if the margin of safe operating conditions is to be properly defined. This was conducted via two main stages: first a deterministic analysis—where the operating conditions are predicted by conventional modelling procedures—and then a probabilistic analysis via stochastic simulations—where a window of optimised operation conditions can be obtained. The outcome of additional stochastic analyses can be used to improve results derived from deterministic models. The incorporation of stochastic techniques in the evaluation of wellbore instability indicates that margins of the safe mud weight window are adjustable and can be extended considerably beyond the limits of deterministic predictions. The safe mud window is influenced and hence can also be amended based on the degree of uncertainty and the permissible level of confidence. The refinement of results from deterministic analyses by additional stochastic simulations is vital if a more accurate and reliable representation of safe in situ and operating conditions is to be obtained during wellbore operations.Published versio

Evidence for the Re-Enactment of a Recently Learned Behavior during Sleepwalking

Animal studies have shown that sequenced patterns of neuronal activity may be replayed during sleep. However, the existence of such replay in humans has not yet been directly demonstrated. Here we studied patients who exhibit overt behaviors during sleep to test whether sequences of movements trained during the day may be spontaneously reenacted by the patients during sleep

Geophysical monitoring of moisture‐induced landslides: a review

Geophysical monitoring of landslides can provide insights into spatial and temporal variations of subsurface properties associated with slope failure. Recent improvements in equipment, data analysis, and field operations have led to a significant increase in the use of such techniques in monitoring. Geophysical methods complement intrusive approaches, which sample only a very small proportion of the subsurface, and walk-over or remotely sensed data, which principally provide information only at the ground surface. In particular, recent studies show that advances in geophysical instrumentation, data processing, modeling, and interpretation in the context of landslide monitoring are significantly improving the characterization of hillslope hydrology and soil and rock hydrology and strength and their dynamics over time. This review appraises the state of the art of geophysical monitoring, as applied to moisture-induced landslides. Here we focus on technical and practical uses of time-lapse methods in geophysics applied to monitoring moisture-induced landslide. The case studies identified in this review show that several geophysical techniques are currently used in the monitoring of subsurface landslide processes. These geophysical contributions to monitoring and predicting the evolution of landslide processes are currently underrealized. Hence, the further integration of multiple-parametric and geotechnically coupled geophysical monitoring systems has considerable potential. The complementary nature of certain methods to map the distribution of subsurface moisture and elastic moduli will greatly increase the predictive and monitoring capacity of early warning systems in moisture-induced landslide settings

Management of anterior cruciate ligament rupture in patients aged 40 years and older

The aim of anterior cruciate ligament (ACL) reconstruction is essentially to restore functional stability of the knee and to allow patients to return to their desired work and activities. While in the young and active population, surgery is often the best therapeutic option after an ACL tear, ACL reconstruction in middle-aged people is rather more controversial due to concerns about a higher complication rate. The purpose of our article is to establish, through a systematic review of the literature, useful decision-making criteria for the management of anterior cruciate ligament rupture in patients aged 40 years and older, guiding surgeons to the most appropriate therapeutic approach. Various reports have shown excellent results of ACL reconstruction in patients over the age of 40 in terms of subjective satisfaction, return to previous activity level, and reduced complication and failure rates. Some even document excellent outcomes in subjects of 50 years and older. Although there are limited high-level studies, data reported in the literature suggest that ACL reconstruction can be successful in appropriately selected, motivated older patients with symptomatic knee instability who want to return to participating in highly demanding sport and recreational activities. Deciding factors are based on occupation, sex, activity level of the subject, amount of time spent performing such highly demanding activities, and presence of associated knee lesions. Physiological age and activity level are more important than chronological age as deciding factors when considering ACL reconstruction

The engineering properties of glacial tills

Glacial tills are a product of the glacial processes of erosion, transportation and deposition and could have been subjected to several glacial cycles and periglacial processes to the extent that they are complex, hazardous soils that are spatially variable in composition, structure, fabric and properties, making them very difficult to sample, test and classify. An overview of the formation of glacial tills and their properties shows that they are composite soils which should be classified according to their lithology, their mode of deposition to link the glacial processes with the facies characteristics and their engineering behaviour. This enables representative design properties to be assigned using frameworks developed for composite soils

Regulation of the Fruit-Specific PEP Carboxylase SlPPC2 Promoter at Early Stages of Tomato Fruit Development

The SlPPC2 phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) gene from tomato (Solanum lycopersicum) is differentially and specifically expressed in expanding tissues of developing tomato fruit. We recently showed that a 1966 bp DNA fragment located upstream of the ATG codon of the SlPPC2 gene (GenBank AJ313434) confers appropriate fruit-specificity in transgenic tomato. In this study, we further investigated the regulation of the SlPPC2 promoter gene by analysing the SlPPC2 cis-regulating region fused to either the firefly luciferase (LUC) or the β-glucuronidase (GUS) reporter gene, using stable genetic transformation and biolistic transient expression assays in the fruit. Biolistic analyses of 5′ SlPPC2 promoter deletions fused to LUC in fruits at the 8th day after anthesis revealed that positive regulatory regions are mostly located in the distal region of the promoter. In addition, a 5′ UTR leader intron present in the 1966 bp fragment contributes to the proper temporal regulation of LUC activity during fruit development. Interestingly, the SlPPC2 promoter responds to hormones (ethylene) and metabolites (sugars) regulating fruit growth and metabolism. When tested by transient expression assays, the chimeric promoter:LUC fusion constructs allowed gene expression in both fruit and leaf, suggesting that integration into the chromatin is required for fruit-specificity. These results clearly demonstrate that SlPPC2 gene is under tight transcriptional regulation in the developing fruit and that its promoter can be employed to drive transgene expression specifically during the cell expansion stage of tomato fruit. Taken together, the SlPPC2 promoter offers great potential as a candidate for driving transgene expression specifically in developing tomato fruit from various tomato cultivars