The young Latin American people of the Cold War

This practice based research study takes a critical memory approach, on the role of documentary production for ethnographic research in post civil war testimonies, and the politics of representation in Central America during the 80’s and today. Researching the memory of those Guatemalans who lived and suffered the impact of the civil war, and how they coped with its repercussions. Examining ‘the subjective act of remembering’ through the case of the Guatemalan artist, filmmaker and activist Ana Lucía Cuevas

A class of quasi-sparse companion pencils

In this paper, we introduce a general class of quasi-sparse potential companion pencils for arbitrary square matrix polynomials over an arbitrary field, which extends the class introduced in [B. Eastman, I.-J. Kim, B. L. Shader, K.N. Vander Meulen, Companion matrix patterns. Linear Algebra Appl. 436 (2014) 255-272] for monic scalar polynomials. We provide a canonical form, up to permutation, for companion pencils in this class. We also relate these companion pencils with other relevant families of companion linearizations known so far. Finally, we determine the number of different sparse companion pencils in the class, up to permutation.This work has been partially supported by theMinisterio de Economía y Competitividad of Spain through grants MTM2015-68805-REDT and MTM2015-65798-P

(R)-(+)-Dimeth­yl[4-oxido-2-oxo-1-(1-phenyl­eth­yl)-1,2,5,6-tetra­hydro­pyridin-3-yl]sulfonium

In the title zwitterionic compound, C15H19NO2S, the six-membered heterocycle adopts a sofa conformation. The negative charge is delocalized along the carbonyl and enolate system on the ring and the positive charge is localized on the S atom. Two inter­molecular C—H⋯O inter­actions help to establish the packing

Towards a FPGA-controlled deep phase modulation interferometer

Deep phase modulation interferometry was proposed as a method to enhance homodyne interferometers to work over many fringes. In this scheme, a sinusoidal phase modulation is applied in one arm while the demodulation takes place as a post-processing step. In this contribution we report on the development to implement this scheme in a fiber coupled interferometer controlled by means of a FPGA, which includes a LEON3 soft-core processor. The latter acts as a CPU and executes a custom made application to communicate with a host PC. In contrast to usual FPGA-based designs, this implementation allows a real-time fine tuning of the parameters involved in the setup, from the control to the post-processing parameters.Comment: Proceedings of the X LISA Symposium, Gainesville, May 18-23, 201

When is a Hamiltonian matrix the commutator of two skew-Hamiltonian matrices?

The mapping (Formula presented.) , where the matrices (Formula presented.) are skew-Hamiltonian with respect to transposition, is studied. Let (Formula presented.) be the range of (Formula presented.) : we give an implicit characterization of (Formula presented.) , obtaining results that find an application in algebraic geometry. Namely, they are used in [R. Abuaf and A. Boralevi, Orthogonal bundles and skew-Hamiltonian matrices, Submitted] to study orthogonal vector bundles. We also give alternative and more explicit characterizations of (Formula presented.) for (Formula presented.). Moreover, we prove that for (Formula presented.) , the complement of (Formula presented.) is nowhere dense in the set of (Formula presented.) -dimensional Hamiltonian matrices, denoted by (Formula presented.) , implying that almost all matrices in (Formula presented.) are in (Formula presented.) for (Formula presented.). Finally, we show that (Formula presented.) is never surjective as a mapping from (Formula presented.) to (Formula presented.) , where (Formula presented.) is the set of (Formula presented.) -dimensional skew-Hamiltonian matrices. Along the way, we discuss the connections of this problem with several existing results in matrix theory

Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.

HIV-specific CD8+ T cells demonstrate an exhausted phenotype associated with increased expression of inhibitory receptors, decreased functional capacity, and a skewed transcriptional profile, which are only partially restored by antiretroviral treatment (ART). Expression levels of the inhibitory receptor, T cell immunoglobulin and ITIM domain (TIGIT), the co-stimulatory receptor CD226 and their ligand PVR are altered in viral infections and cancer. However, the extent to which the TIGIT/CD226/PVR-axis is affected by HIV-infection has not been characterized. Here, we report that TIGIT expression increased over time despite early initiation of ART. HIV-specific CD8+ T cells were almost exclusively TIGIT+, had an inverse expression of the transcription factors T-bet and Eomes and co-expressed PD-1, CD160 and 2B4. HIV-specific TIGIThi cells were negatively correlated with polyfunctionality and displayed a diminished expression of CD226. Furthermore, expression of PVR was increased on CD4+ T cells, especially T follicular helper (Tfh) cells, in HIV-infected lymph nodes. These results depict a skewing of the TIGIT/CD226 axis from CD226 co-stimulation towards TIGIT-mediated inhibition of CD8+ T cells, despite early ART. These findings highlight the importance of the TIGIT/CD226/PVR axis as an immune checkpoint barrier that could hinder future "cure" strategies requiring potent HIV-specific CD8+ T cells

Human Immunodeficiency Virus type 1 in seronegative infants born to HIV-1-infected mothers

BACKGROUND: Some individuals repeatedly exposed to Human Immunodeficiency Virus do not seroconvert and are resistant to HIV infection. Here, in a pediatric cohort of HIV seronegative infants born of HIV-infected mothers, we have studied eight non-breastfed children in whom viral DNA was detected in their PBMC. Our objective was to assess whether silent infection in these children can be explained by the presence of integrated viral DNA. METHODS: The presence of viral DNA was corroborated by nested PCR with primers for gag and the nef/LTR regions of HIV-1. Integration of HIV DNA into the host genome was assessed by an Alu-LTR PCR. Amplicons were sequenced and phylogenetic analyzes were done. RESULTS: HIV-1 DNA was detected in the earliest available PBMC sample from all eight infants, and two of them tested positive for HIV DNA at 2 years of age. Nested PCR resulted in the amplification of gag, nef/LTR and Alu-LTR fragments, which demostrated that HIV-1 DNA was integrated in the host cell genome. Each individual has a characteristic sequence pattern and is different from the LTR sequence of HXB2 prototype virus and other Mexican isolates. CONCLUSION: HIV-1 DNA was observed in PBMC from HIV exposed seronegative children in this pediatric cohort

In Situ Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization.

CD4 T cells are key mediators of adaptive immune responses during infection and vaccination. Within secondary lymphoid organs, helper CD4 T cells, particularly those residing in germinal centers known as follicular helper T cells (Tfh), provide critical help to B-cells to promote their survival, isotype switching and selection of high affinity memory B-cells. On the other hand, the important role of Tfh cells for the maintenance of HIV reservoir is well documented. Thus, interrogating and better understanding the tissue specific micro-environment and immune subsets that contribute to optimal Tfh cell differentiation and function is important for designing successful prevention and cure strategies. Here, we describe the development and optimization of eight multispectral confocal microscopy immunofluorescence panels designed for in depth characterization and immune-profiling of relevant immune cells in formalin-fixed paraffin-embedded human lymphoid tissue samples. We provide a comprehensive library of antibodies to use for the characterization of CD4+ T-cells -including Tfh and regulatory T-cells- as well as CD8 T-cells, B-cells, macrophages and dendritic cells and discuss how the resulting multispectral confocal datasets can be quantitatively dissected using the HistoCytometry pipeline to collect information about relative frequencies and immune cell spatial distributions. Cells harboring actively transcribed virus are analyzed using an in-situ hybridization assay for the characterization of HIV mRNA positive cells in combination with additional protein markers (multispectral RNAscope). The application of this methodology to lymphoid tissues offers a means to interrogate multiple relevant immune cell targets simultaneously at increased resolution in a reproducible manner to guide CD4 T-cell studies in infection and vaccination

Entre mitos y realidades de la justicia penal de la niñez y la adolescencia en Centroamérica

El trabajo presenta un estudio sobre los avances de la adecuación legislativa de las diferentes legislaciones interna de los países de Centroamérica a la Convención de Naciones Unidas sobre los Derechos del Niño, reformas, contramarchas y riesgos regionales en esta materia. El trabajo además presenta, en la medida de lo posible, un análisis sociológico de la criminalidad y la violencia juvenil, sus características, extensiones, mitos y realidades y las reacciones punitivas.The work presents a study on the progress of the legislative adaptation of the different internal legislations of the Central American countries to the United Nations Convention on the Rights of the Child, reforms, counter marches and regional risks in this matter. The work also presents, as far as possible, a sociological analysis of youth crime and violence, its characteristics, extensions, myths and realities, and punitive reactions

Unilateral submandibular gland aplasia with ipsilateral sublingual gland hypertrophy presenting as a neck mass

The congenital absence off the major salivary glands is a very infrequent disorder, in which several glands are usually involved at the same time. Sometimes this disorder can be associated with other developmental anomalies. The unilateral aplasia of the submandibular gland is an extremely rare finding with only 14 cases reported in the literature. Clinically, this kind of patients may complain of dryness of the mouth, difficulties in chewing and swallowing, severe periodontal disease or multiple caries, but usually they follow an asymptomatic course. Salivary gland aplasia can be diagnosed with a large variety off imaging techniques, which include computer tomography (CT), magnetic resonance imaging (MRR), ultrasonography (UUS), sialography, or scintigraphy. In this paper we report a case off a patient referred to our department with a long term and progressive growing neck mass, who has an unilateral submandibular gland aplasia associated to an ipsilateral hypertrophy off the sublingual gland. © Medicina Oral S. L