19 research outputs found

    Bayesian spatio-temporal CPUE standardization: Case study of European sardine (Sardina pilchardus) along the western coast of Portugal

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    Understanding the key factors influencing population dynamics of fish stocks requires knowledge of their spatial distribution and seasonal habitat selection, but these spatio-temporal dynamics are often not explicitly included in ecological studies and stock assessment models. This study standardized the data of sardine fishery-dependent catch-per- unit- effort (CPUE) from the west coast of Portugal using Bayesian hierarchical spatio-temporal models (BHSTM) with the integrated nested Laplace approximation (INLA). Sardine CPUE was best explained by length of the vessel, vessel ID, month, year, and location (latitude, longitude). In terms of spatio-temporal distribution, sardine biomass prediction maps showed a constant pattern that changed every quarter of the year. In addition, sardine CPUE index showed a cyclical trend along the year with minimum values in July and maximum peak in November. This approach provided insights on variables and corresponding modelling effects that may be relevant in spatio-temporal fishery-dependent data standardization, and that could be applied to other fish species and areas.En prens

    Bayesian spatio-temporal CPUE standardization: case study of European sardine (Sardina pilchardus) along the western coast of Portugal

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    Fishery data is one of the most accessible sources of information currently used for ecological studies and stock assessments. Unlike scientific surveys that are usually restricted to a given time of the year, fisheries dependent data is almost continuously available in time. Moreover, the information collected from the fisheries is less expensive and time consuming. However, for use as a relative abundance index, fishery-dependent data requires standardization as catch-per-unit-effort (CPUE) in order to remove the impact of vessel-specific differences and fishing behavior. Understanding the key factors that influence the population dynamics of fish species implies assessment of their spatial distribution and seasonal habitat selection but, spatio-temporal dependence issues are often not explicitly included in the modeling process. This study standardizes sardine fishery-dependent data obtained from the west coast of Portugal as CPUE by means of a Bayesian hierarchical spatio-temporal model using integrated nested Laplace approximation (INLA). This is one of the first studies of the region to provide maps of the relative abundance of this species for all months of the year. The best model included length of the vessel, vessel ID, month, year and location (latitude, longitude), while none of the five environmental covariates (Chl-a, SST, bathymetry, current velocity and direction) were relevant. In terms of spatial distribution, sardines were more abundant in the northern area, especially during the last quarter of the year. The applied methodology has contributed to improve our knowledge of European sardine distribution throughout the year, providing accurate predictive maps and insights into the standardization process of fishery-dependent data that could also be applied to other fish species and areas

    Oral vaccination of fish against vibriosis using spore-display technology

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    Publisher Copyright: Copyright © 2022 Gonçalves, Santos, Coutinho, Pedrosa, Curado, Machado, Costas, Bonneville, Serrano, Carvalho, Díaz-Rosales, Oliva-Teles, Couto and Serra.Oral vaccines are highly demanded by the aquaculture sector, to allow mass delivery of antigens without using the expensive and labor-intensive injectable vaccines. These later require individual handling of fish, provoking stress-related mortalities. One possible strategy to create injection-free vaccine delivery vehicles is the use of bacterial spores, extremely resistant structures with wide biotechnological applications, including as probiotics, display systems, or adjuvants. Bacterial spores, in particular those of Bacillus subtilis, have been shown to behave as mucosal vaccine adjuvants in mice models. However, such technology has not been extensively explored against fish bacterial disease. In this study, we used a laboratory strain of B. subtilis, for which a variety of genetic manipulation tools are available, to display at its spores surface either a Vibrio antigenic protein, OmpK, or the green fluorescence protein, GFP. When previously vaccinated by immersion with the OmpK- carrying spores, zebrafish survival upon a bacterial challenge with V. anguillarum and V. parahaemolyticus, increased up to 50 - 90% depending on the pathogen targeted. Further, we were able to detect anti-GFP-antibodies in the serum of European seabass juveniles fed diets containing the GFP-carrying spores and anti-V. anguillarum antibodies in the serum of European seabass juveniles fed the OmpK-carrying spores containing diet. More important, seabass survival was increased from 60 to 86% when previously orally vaccinated with in-feed OmpK- carrying spores. Our results indicate that B. subtilis spores can effectively be used as antigen-carriers for oral vaccine delivery in fish.publishersversionpublishe

    Evaluation of SMOS L4 Sea Surface Salinity Product in the Western Iberian Coast

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    Special issue Moving Forward on Remote Sensing of Sea Surface Salinity.-- 24 pages, 14 figures, supplementary materials https://www.mdpi.com/article/10.3390/rs14020423/s1.-- Data Availability Statement: Data sharing not applicableSalinity is one of the oldest parameters being measured in oceanography and one of the most important to study in the context of climate change. However, its quantification by satellite remote sensing has been a relatively recent achievement. Currently, after over ten years of data gathering, there are still many challenges in quantifying salinity from space, especially when it is intended for coastal environments study. That is mainly due to the spatial resolution of the available products. Recently, a new higher resolution (5 km) L4 SMOS sea surface salinity (SSS) product was developed by the Barcelona Expert Center (BEC). In this study, the quality of this product was tested along the Western Iberian Coast through its comparison with in situ observations and modelled salinity estimates (CMEMS IBI Ocean Reanalysis system). Moreover, several parameters such as the temperature and depth of in situ measurements were tested to identify the variables or processes that induced higher errors in the product or influenced its performance. Lastly, a seasonal and interannual analysis was conducted considering data between 2011 to 2019 to test the product as a potential tool for long-term studies. The results obtained in the present analysis showed a high potential of using the L4 BEC SSS SMOS product in extended temporal and spatial analyses along the Portuguese coast. A good correlation between the satellite and the in situ datasets was observed, and the satellite dataset showed lower errors in retrieving coastal salinities than the oceanic model. Overall, the distance to the coast and the closest rivers were the factors that most influenced the quality of the product. The present analysis showed that great progress has been made in deriving coastal salinity over the years and that the SMOS SSS product is a valuable contribution to worldwide climatological studies. In addition, these results reinforce the need to continue developing satellite remote sensing products as a global and cost-effective methodology for long-term studiesThis work was conducted within the framework of the project AQUIMAR—Marine Knowledge Supporting Aquaculture (MAR-02.01.01-FEAMP-0107), funded by the Mar 2020—Operational Program Mar2020. B.B. was funded by a grant from Mar2020 under AQUIMAR project and also by a PhD grant awarded by Fundação para a Ciência e a Tecnologia (FCT) within the scope of the MIT Portugal Program. A.C.B. was funded by FCT through the Scientific Employment Stimulus Programme (CEECIND/0095/2017). A.T. was funded by Project SARDINHA2020 (MAR-01.04.02-FEAMP-0009), funded by the Operational Program Mar2020. This work benefited from the Infrastructure CoastNet (http://geoportal.coastnet.pt, accessed on 30 September 2021), funded by FCT and the European Regional Development Fund (FEDER), through LISBOA2020 and ALENTEJO2020 regional operational programs, in the framework of the National Roadmap of Research Infrastructures of strategic relevance (PINFRA/22128/2016). This study also received further support from FCT through MARE’s strategic program (UID/MAR/04292/2019). This work represents a contribution to CSIC Thematic Interdisciplinary Platform PTI Teledetect, with the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S). This publication was also funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement N810139: Project Portugal Twinning for Innovation and Excellence in Marine Science and Earth Observation—PORTWIMSPeer reviewe

    Diabetes as an independent predictor of high atherosclerotic burden assessed by coronary computed tomography angiography: The coronary artery disease equivalent revisited

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    (1) To study the prevalence and severity of coronary artery disease (CAD) in diabetic patients. (2) To provide a detailed characterization of the coronary atherosclerotic burden, including the localization, degree of stenosis and plaque composition by coronary computed tomography angiography (CCTA). Single center prospective registry including a total of 581 consecutive stable patients (April 2011-March 2012) undergoing CCTA (Dual-source CT) for the evaluation of suspected CAD without previous myocardial infarction or revascularization procedures. Different coronary plaque burden indexes and plaque type and distribution patterns were compared between patients with (n = 85) and without diabetes (n = 496). The prevalence of CAD (any plaque; 74.1 vs. 56 %; p = 0.002) and obstructive CAD (≥50 % stenosis; 31.8 vs. 10.3 %; p<0.001) were significantly higher in diabetic patients. The remaining coronary atherosclerotic burden indexes evaluated (plaque in LM-3v-2v with prox. LAD; SIS; SSS; CT-LeSc) were also significantly higher in diabetic patients. In the per segment analysis, diabetics had a higher percentage of segments with plaque in every vessel (2.6/13.1/7.5/10.5 % for diabetics vs. 1.4/7.1/3.3/4.4 % for nondiabetics for LM, LAD, LCx, RCA respectively; p<0.001 for all) and of both calcified (19.3 vs. 9.2 %, p<0.001) and noncalcified or mixed types (14.4 vs. 7.0 %; p<0.001); the ratio of proximal-to-distal relative plaque distribution (calculated as LM/proximal vs. mid/distal/branches) was lower for diabetics (0.75 vs. 1.04; p = 0.009). Diabetes was an independent predictor of CAD and was also associated with more advanced CAD, evaluated by indexes of coronary atherosclerotic burden. Diabetics had a significantly higher prevalence of plaques in every anatomical subset and for the different plaque composition. In this report, the relative geographic distribution of the plaques within each subgroup, favored a more mid-to-distal localization in the diabetic patients

    Coronary computed tomography angiography-adapted Leaman score as a tool to noninvasively quantify total coronary atherosclerotic burden

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    To describe a coronary computed tomography angiography (CCTA)-adapted Leaman score (CT-LeSc) as a tool to quantify total coronary atherosclerotic burden with information regarding localization, type of plaque and degree of stenosis and to identify clinical predictors of a high coronary atherosclerotic burden as assessed by the CT-LeSc. Single center prospective registry including a total of 772 consecutive patients undergoing CCTA (Dual-source CT) from April 2011 to March 2012. For the purpose of this study, 581 stable patients referred for suspected coronary artery disease (CAD) without previous myocardial infarction or revascularization procedures were included. Pre-test CAD probability was determined using both the Diamond-Forrester extended CAD consortium method (DF-CAD consortium model) and the Morise score. Cardiovascular risk was assessed with the HeartScore. The cut-off for the 3rd tercile (CT-LeSc ≥8.3) was used to define a population with a high coronary atherosclerotic burden. The median CT-LeSc in this population (n = 581, 8,136 coronary segments evaluated; mean age 57.6 ± 11.1; 55.8 % males; 14.6 % with diabetes) was 2.2 (IQR 0-6.8). In patients with CAD (n = 341), the median CT-LeSc was 5.8 (IQR 3.2-9.6). Among patients with nonobstructive CAD, most were classified in the lowest terciles (T1, 43.0 %; T2, 36.1 %), but 20.9 % were in the highest tercile (T3). The majority of the patients with obstructive CAD were classified in T3 (78.2 %), but 21.8 % had a CT-LeSc in lower terciles (T1 or T2). The independent predictors of a high CT-LeSc were: Male sex (OR 1.73; 95 % CI 1.04-2.90) diabetes (OR 2.91; 95 % CI 1.61-5.23), hypertension (OR 2.54; 95 % CI 1.40-4.63), Morise score ≥16 (OR 1.97; 95 % CI 1.06-3.67) and HeartScore ≥5 (OR 2.42; 95 % CI 1.41-4.14). We described a cardiac CT adapted Leaman score as a tool to quantify total (obstructive and nonobstructive) coronary atherosclerotic burden, reflecting the comprehensive information about localization, degree of stenosis and type of plaque provided by CCTA. Male sex, hypertension, diabetes, a HeartScore ≥5 % and a Morise score ≥16 were associated with a high coronary atherosclerotic burden, as assessed by the CT-LeSc. About one fifth of the patients with nonobstructive CAD had a CT-LeSc in the highest tercile, and this could potentially lead to a reclass

    RELATO DE VISITA TÉCNICA AO ASSENTAMENTO JOSÉ MARIA – PROBLEMATIZANDO A EDUCAÇÃO NO/DO CAMPO

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    O relato de Experiência a ser compartilhado com os participantes do II MEPEC será organizado no formato de “sessões de apresentação” com apoio de audiovisual. Os autores exporão aos visitantes a vivência ocorrida em setembro de 2016 durante visita técnica ao Campus Avançado do IFC em Abelardo Luz, situado no AssentamentoJosé Maria.Esta visita teve como objetivos:-Compreender o que representa a expressão “sem terra”, de modo a extrapolar a ideia de mera luta pela terra e entender que “sem terra” é uma identidade historicamente construída como afirmação de uma condição social (CALDART, 2001);-Conhecer o movimento pedagógico de uma escola que atua para a formação de sujeitos sociais e de seres humanos que têm a identidade de luta como elemento que os aproxima, a Escola de Ensino Médio Paulo Freire;-Olhar para o Movimento Sem Terra e sua trajetória, em especial, no que diz respeito à Pedagogia e à educação das crianças, jovens e adultos pertencentes ao Movimento, e pensar que em tempos de desumanidade crescente há que investir em processos de formação humana marcados pela alteridade, pelo reconhecimento do outro enquanto um outro diferente de mim;-Compreender a influência e a importância dos movimentos sociais na formação da consciência social do direito a Educação Básica, desde a Educação Infantil;-o direito à escola pública.-Aproximar as/os acadêmicas/os da realidade dos movimentos sociais, de modo que esta relação teoria-prática faça emergir sentidos que contribuam para problematizar conhecimentos produzidos no âmbito dos componentes curriculares do Curso de Pedagogia.A apresentação na II MEPEC está organizada em torno de quatro temas:-Vida de assentado: breve história, as lutas e conquistas, a situação atual…-O Campus avançado do IFC em Abelardo Luz;-Escola do/no campo: o ensino fundamental (as escolas municipais);-Escola do/no campo: o ensino médio (as escolas estaduais).No decorrer da apresentação dos temas serão utilizadas imagens (fotos) e entrevistas realizadas com pessoas do local, para o que se fará necessário contar com Datashow e computador e razão pela qual haverá mais de um expositor

    OFICINAS DO BRINCAR: UM RESGATE A INFÂNCIA POR MEIO DE JOGOS, BRINQUEDOS E BRINCADEIRAS. (relato de experiência)

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    A brincadeira e jogo são construções sociais e, portanto culturais. A criança ao nascer está impregnada de práticas estabelecidas nas relações com outros sujeitos, adultos e outras crianças que possibilitam o acesso aos códigos sociais estabelecidos na forma de aprendizagem social. É na brincadeira, na fantasia e posteriormente no jogo com a elaboração de regras mais complexas que a criança se apropria da organização social na qual está inserida. Como processo cultural, o acesso a brincadeira e ao jogo se dá fundamentalmente na relação com outro e com as condições de espaço, materiais e possibilidades de exploração desses elementos. Considerando a importância da brincadeira e do jogo em proposições criativas, de descobertas, elaborações ereelaborações, estamos realizando um projeto de pesquisa denominado “Oficinas do brincar: um resgate a infância por meio de jogos, brinquedos e brincadeiras”, desenvolvido com os acadêmicos da quarta fase do curso de Licenciatura em Pedagogia, do Instituto Federal Catarinense – campi Blumenau (SC), com a perspectiva de propiciar espaço de relacionamento significativo com a brincadeira, o jogo, o brinquedo e práticas corporais expressivas como elementos de acesso a cultura lúdica e, portanto de inserção num contexto social humanizador. A dinâmica do projeto compreende atividades sequenciais com os discentes, semanalmente, nos quais são utilizadas as dependências da própria instituição, que disponibiliza o espaço físico e conta com a colaboração dos estudantes ao trazerem os recursos - como brinquedos, jogos e materiais para práticas corporais e expressivas. O projeto está em fase inicial de desenvolvimento, mas vem confirmando a relevância de seus propósitos, haja visto que parte do princípio de propiciar resgate e vivências da infância, do brincar, através dos jogos, brinquedos e brincadeiras, tematizando discussões em torno da ludicidade, como processo histórico e social, capaz de proporcionar reflexão e colaborar com a formação humana pelo acesso e interação com essas formas de manifestação da cultura. Dessa forma, objetiva-se com esta oficina, propiciar espaço e oportunidades de autoconhecimento, vivências inclusivas, melhora da auto-estima, desenvolver habilidades de comunicação, expressão e interação social dos acadêmicos envolvidos no projeto com os sujeitos participantes, colaborando com a formação acadêmica dos alunos mediadores do projeto tanto no que se refere à prática pedagógica exercida sob a perspectiva de professor, intelectual-pesquisador, bem como para a comunidade envolvida, no sentido de poder recordar os jogos, brinquedos e brincadeiras de suas infâncias por meio de cantos temáticos (num estande ou sala de aula), contendo: mostra do acervo de brinquedos que fizeram parte da infância da turma; produção artesanal de brinquedos sustentáveis com matérias recicláveis; espaço do faz de conta, com oficineiros desenvolvendo atividades recreacionistas para o público visitante

    Educomunicação e suas áreas de intervenção: Novos paradigmas para o diálogo intercultural

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    oai:omp.abpeducom.org.br:publicationFormat/1O material aqui divulgado representa, em essência, a contribuição do VII Encontro Brasileiro de Educomunicação ao V Global MIL Week, da UNESCO, ocorrido na ECA/USP, entre 3&nbsp;e 5 de novembro de 2016. Estamos diante de um conjunto de 104 papers executivos, com uma média de entre 7 e 10 páginas, cada um. Com este rico e abundante material, chegamos ao sétimo e-book publicado pela ABPEducom, em seus seis primeiros anos de existência. A especificidade desta obra é a de trazer as “Áreas de Intervenção” do campo da Educomunicação, colocando-as a serviço de uma meta essencial ao agir educomunicativo: o diálogo intercultural, trabalhado na linha do tema geral do evento internacional: Media and Information Literacy: New Paradigms for Intercultural Dialogue

    Surface circulation in the Gulf of Cadiz: 2. Inflow-outflow coupling and the Gulf of Cadiz slope current

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    0148-0227International audienceA study of the upper slope circulation in the Gulf of Cadiz is presented. Observations, both original and revisited, and realistic numerical modeling are used together to describe the structure and variability of the slope current system above the Mediterranean outflow. It is shown that the Mediterranean inflow-outflow coupling plays a stronger role than that of the atmospheric forcing in driving the upper slope currents. The Mediteranean outflow forces a surface open ocean current toward the Strait of Gibraltar. Part of it is entrained into the outflow and the remaining flows into the Mediterranean. This latter component does not suffice for the observed transport of the Atlantic inflow into the Mediterranean. A secondary contribution to the inflow is therefore needed to complement the transport. This contribution comes from a persistent equatorward current along the upper slope between Cape St. Vincent and the Strait of Gibraltar. The jet is 20-30 km wide and significant in the upper 200 m attaining subinertial maxima as much as 0.3-0.4 m/s and monthly means in the order of 0.1-0.15 m/s. This current shows a strong variability at time scales in the order of 2-8 days, and displays a significant vertical shear. The response of the upper slope current to synoptic and seasonal atmospheric variability is analyzed. Very low correlation was detected at synoptic scales and the response of the system to seasonal forcing is unclear. A cycle of intensification in June-July and a decrease in winter is apparent in the measurements, but is weak in the model results. It is speculated that the cycle in the observed currents is associated with variability in the inflow/outflow coupling system, rather than driven by seasonally changing wind forcing
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