Prolactin receptor (PRLR) gene polymorphism in Chios, White Karaman and Awassi sheep breeds

The objective of the present study was to determine the polymorphism in the prolactine receptor (PRLR) gene in Chios, White Karaman and Awassi, which are native sheep breeds in Turkey. By means of PRLR gene sequence homology between sheep and humans, two primer pairs were designed for polymerase chain reaction (PCR) amplification within intron 1 and exon 10 of the PRLR gene in sheep. A total of 160 amplicons (99 for intron 1 and 61 for exon 10) were subjected to DNA sequence analysis. For intron 1, 6 different haplotypes were determined. For exon 10, 7 different haplotypes were obtained. Some variations determined for exon 10 (g.14A>T p.Q14L; g.160G>A p.D160N; g.166G>A p.E166K; g.167A>T p.E167V; g.176A>T p.H176L; g.206G>A p.S206N; g.208G>A p.G208R) led to changes in the amino acids, but no amino acid changes were determined in g.2A>T, g.81A>G, g.138A>G, g.186C>T, g..207T>C. It was noted in particular that White Karaman and Awassi were similar to each other in both PRLR exon 10 and intron 1 haplotypes, whereas the Chios breed had a different variation.TubitakTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Council of Firat UniversityFirat University [1070894]The sequence experiments were carried out at the Ankara University Biotechnology Institute Genomics Unit. The Project was supported by Tubitak and Scientific Research Projects Council of Firat University (Project code Tubitak 1070894; Fubap: 1502)

TURKISH JOURNAL of ONCOLOGY Gastrointestinal Stromal Tumors: A Single Center Experience

OBJECTIVE Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumor of the gastrointestinal (GI) tract. The aim of this retrospective study was to explore the characteristics, prognostic factors, and treatment results of GIST cases. METHODS Clinical and pathological data of 35 GIST patients at our center between 2002 and 2015 were reviewed. RESULTS Total of 18 (51.4%) were women and 17 (48.6%) were men, with median age of 54 years. Common site of tumor was stomach (48.6%). Abdominal pain (37.1%) was common clinical symptom. Risk group distribution was 8.6% low, 31.4% intermediate, and 60% high-risk cases. Mean follow-up period of the patients was 34 months. Low-risk GIST can be treated with surgery alone. Recurrence was observed in only 1 of 10 patients who received adjuvant treatment. All 6 patients in whom metastasis was determined were in high-risk group, and 4 of them had liver metastasis. Metastasis was not detected in any of the patients who had <5 mitoses per 50 high-power field (HPF), but in 5 of 12 patients who had >10 mitoses per 50 HPF, metastasis was determined. Metastasis did not correlate with site or size of tumor, but was related to high mitotic rate (p=0.015). Median overall survival of the patients was 79 months. CONCLUSION Low-risk GIST can be treated with surgery alone. Imatinib therapy significantly improves survival of high-risk or advanced-stage GIST patients. Metastasis did not correlate with site or size of tumor, but correlation with high mitotic rate was observed

Fundamental open questions on engineering of "super" hydrogen sorption in graphite nanofibers: relevance for clean energy applications

Herein, some fundamental open questions on engineering of “super” hydrogen sorption (storage) in carbonaceous nanomaterials are considered, namely: 1) on thermodynamic stability and related characteristics of some hydrogenated graphene layers nanostructures: relevance to the hydrogen storage problem; 2) determination of thermodynamic characteristics of graphene hydrides; 3) a treatment and interpretation of some recent STM, STS, HREELS/LEED, PES, ARPS and Raman spectroscopy data on hydrogensorbtion with epitaxial graphenes; 4) on the physics of intercalation of hydrogen into surface graphene-like nanoblisters in pyrolytic graphite and epitaxial graphenes; 5) on the physics of the elastic and plastic deformation of graphene walls in hydrogenated graphite nanofibers; 6) on the physics of engineering of “super” hydrogen sorption (storage) in carbonaceous nanomaterials, in the light of analysis of the Rodriguez-Baker extraordinary data and some others. These fundamental open questions may be solved within several years

Cavernous Hemangioma-Like Kaposi Sarcoma: Histomorphologic Features and Differential Diagnosis

Aim. Cavernous hemangioma-like Kaposi sarcoma is a rare morphologic type of Kaposi sarcoma. So far there are no cases in the literature defining the histological features of this morphologic spectrum in detail. In this study we presented two classical-type cutaneous Kaposi sarcoma cases with histologic findings resembling cavernous hemangioma in company with clinical and histopathological data. Cases. One hundred and eighty-five classical-type cutaneous Kaposi sarcoma lesions in 79 patients were assessed retrospectively in terms of histopathological features. Findings of two cases showing features of cavernous hemangioma-like Kaposi sarcoma whose clinical data could be accessed were presented in accompany with the literature data. Both cases were detected to have bluish-purple, protruded, irregularly bordered cutaneous lesions. Histopathological examination revealed a lesion formed by cavernous hemangioma-like vascular structures organized in a lobular pattern that became dilated and filled with blood. Typical histological findings of early-stage KS, consisting of mononuclear inflammation, extravasated erythrocytes, and a few immature vascular structures in superficial dermis, were observed. All cases were serologically HIV-1 negative. A positive reaction with HHV-8, CD31, CD34, and D2-40 monoclonal antibodies was identified at both cavernous hemangioma-like areas and in immature vascular structures. Results. Cavernous hemangioma-like Kaposi sarcoma is a rare Kaposi sarcoma variant presenting with diagnostic challenges, that may be confused with hemangioma. As characteristic morphological features may not be observed in every case, it is important for diagnostic purposes to show immunohistochemical HHV-8 positivity in this variant

Yaşlılara bakım verenlerde tükenmi̇şli̇k ve i̇lgi̇li̇ faktörler: Yaşlı bakımevleri̇nde çalışanları neler etki̇ler?

Introduction: People directly engaged in elderly care can suffer from stress, burnout and related disorders. The aim of this study was to examine the burnout levels of the nursing staff working in an elderly nursing home and its relationship with their self-efficacy, coping strategies and general well-being. Materials and Method: A cross-sectional correlation study was carried out by means of the Individual Information Form, the Maslach Burnout Inventory, the Self-Efficacy Scale, the General Health Questionnaire and the Ways of Coping Questionnaire. Hierarchical regression analysis was also conducted to identify the predictors of dimensions of burnout. The relationship between the variables was examined by Pearson correlation coefficient using SPSS 11.0 statistics program. Results: Mean burnout scores of the participants were 0.78 +/- 0.66 (Min 0, Max 3.2) for emotional exhaustion, 0.39 +/- 0.51 (Min 0, Max 2.2) for depersonalization and 2.99 +/- 0.69 (Min 0, Max 4.0) for personal accomplishment. Conclusion: The results showed that coping strategies and self-efficacy are related to the burnout levels of the caregivers in elderly nursing homes. For better and healthy long term care of the elderly, caregivers have to be given professional and psychological support

A family with hereditary angioedema having been followed as familial Mediterranean Fever

Herediter anjioödem fonksiyonel C1 esteraz inhibitör proteinin konjenital eksikliği sonucu oluşan, otozomal dominant kalıtılan nadir bir hastalıktır. Herediter anjioödemli hastalar klinik olarak yüz, gövde, solunum yolları, abdominal organları ve ekstremiteleri etkileyen tekrarlayan ödem epizodları ile karakterizedir. Ataklar ya spontan olarak ya da stres veya travmayı takiben oluşabilir. Hastalık sıklıkla karın ağrısı atakları ile seyreder. Öyle ki hastalar bu şikayet nedeniyle dermatoloji kliniğinden ziyade diğer kliniklere başvurabilir ve yanlış tanı konulup takip edilebilir. Bu nedenle karın ağrısından yakınan olgularda ayırıcı tanıda herediter anjioödem de düşünülmelidir. Bu yazıda uzun süredir Ailevi Akdeniz Ateşi olarak takip edilen herediter anjioödem tanılı bir aile sunmaktayız. Aile bireyleri çocukluk çağından beri vücudunun değişik bölgelerinde devamlı oluşup sonra kaybolan şişliklerden ve şiddetli karın ağrısından yakınmaktaydı. Hastalar yanlışlıkla Ailevi Akdeniz Ateşi tanısı ile yıllarca izlenmiş ve tedavi edilmeye çalışılmıştır. Olgulara kliniğimizde herediter anjioödem tanısı konuldu ve danazol tedavisinden fayda gördüler.Hereditary angioedema is a rare autosomal dominant disorder resulting from the congenital deficiency of functional C1 esterase inhibitor protein. Patients with hereditary angioedema are clinically characterized by recurrent episodes of swelling of the extremities, face, trunk, airways and abdominal viscera. Attacks may occur either spontaneously or following stress or trauma. The disease is usually associated with attacks of abdominal pain. So, patients may apply for this complaint to other clinics rather than dermatology, and may be misdiagnosed and followed for a long time. Therefore hereditary angioedema should be thought in differential diagnosis of patients suffering from abdominal pain. Here in this writing, we describe a family with hereditary angioedema who has been followed as Familial Mediterranean Fever for a long time. The family members complained from swellings which have been occuring in various regions of the body and disappearing spontaneously, and complained from severe abdominal pain, since childhood. These patients have been followed and tried to be treated with the misdiagnosis of Familial Mediterranean Fever for many years. These patients were diagnosed as hereditary angioedema in our clinic, and benefited from danazol treatment

BURNOUT AND RELEVANT FACTORS IN NURSING STAFF: WHAT AFFECTS THE STAFF WORKING IN AN ELDERLY NURSING HOME?

Introduction: People directly engaged in elderly care can suffer from stress, burnout and related disorders. The aim of this study was to examine the burnout levels of the nursing staff working in an elderly nursing home and its relationship with their self-efficacy, coping strategies and general well-being