5-Hydroxymethylfurfural content in foodstuffs determined by micellar electrokinetic chromatography

Micellar electrokinetic chromatography (MEKC) has been applied for the determination of 5-hydroxymethylfurfural in several foodstuffs. A 75 mM phosphate buffer solution at pH 8.0 containing 100 mM sodium dodecylsulfate was used as background electrolyte (BGE), and the separation was performed by applying +25 kV in a 50 µm I.D. uncoated fused-silica capillary. Good linearity over the range 2.5-250 mg kg-1 (r2 ≥ 0.999) and run-to-run and day-to-day precisions at low and medium concentration levels were obtained. Sample limit of detection (0.7 mg kg-1) and limit of quantification (2.5 mg kg-1) were established by preparing the standards in blank matrix. The procedure was validated by comparing the results with those obtained with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Levels of HMF in 45 different foodstuffs such as breakfast cereals, toasts, honey, orange juice, apple juice, jam, coffee, chocolate and biscuits were determined

5-Hydroxymethylfurfural content in foodstuffs determined by micellar electrokinetic chromatography

Micellar electrokinetic chromatography (MEKC) has been applied for the determination of 5-hydroxymethylfurfural in several foodstuffs. A 75 mM phosphate buffer solution at pH 8.0 containing 100 mM sodium dodecylsulfate was used as background electrolyte (BGE), and the separation was performed by applying +25 kV in a 50 µm I.D. uncoated fused-silica capillary. Good linearity over the range 2.5-250 mg kg-1 (r2 ≥ 0.999) and run-to-run and day-to-day precisions at low and medium concentration levels were obtained. Sample limit of detection (0.7 mg kg-1) and limit of quantification (2.5 mg kg-1) were established by preparing the standards in blank matrix. The procedure was validated by comparing the results with those obtained with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Levels of HMF in 45 different foodstuffs such as breakfast cereals, toasts, honey, orange juice, apple juice, jam, coffee, chocolate and biscuits were determined

Thanks mum. Maternal effects in response to ocean acidification of sea urchin larvae at different ecologically relevant temperatures

International audienceJuvenile stages of marine species might be more vulnerable than adults to climate change, however larval vulnerability to predictable environmental changes can be mitigated by parental anticipatory buffer effects occurring during gametogenesis. In this study, ocean acidification effect were investigated on larval growth of two sea urchins, Arbacia lixula and Paracentrotus lividus, at different temperature levels. Results showed that altered pH and temperature affected larval development in both species, with significant length reductions of spicules and significant increases in abnormal larvae. Detrimental effects of reduced pH and high temperature were however dependent on the mother. Furthermore, the responses of A. lixula larvae from the ambient site (pH ∼ 8.0) were compared with those of larvae obtained from mothers collected from a natural CO2 vent (pH ∼ 7.7) in Ischia. Comparisons highlighted a transgenerational response, as the CO2 vent larvae proved to be more resilient to reduced pH, although more sensitive to increased temperature

Interferon gamma, an important marker of response to immune checkpoint blockade in non-small cell lung cancer and melanoma patients

Background: Programmed death-ligand 1 (PD-L1) may be induced by oncogenic signals or can be upregulated via interferon gamma (IFN-y). We have explored whether the expression of IFNG, the gene encoding IFN-y, is associated with clinical response to the immune checkpoint blockade in non-small cell lung cancer (NSCLC) and melanoma patients. The role of inflammation-associated transcription factors STAT3, IKBKE, STAT1 and other associated genes has also been examined