A Herpetofauna Do Parque Nacional Da Serra Das Confusões, Piauí, Brasil, Com Uma Lista Regional Para Uma área Ecotonal Entre O Cerrado E A Caatinga

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Different physiognomies at Parque Nacional da Serra das Confusões (PNSCo) were intensively sampled aiming to access the distribution pattern of its herpetofauna. Sixty six species were found in the park (47 reptiles and 19 amphibians); the rarefaction curve for lizards, although not fully stabilized in an asymptote, indicates that the sampling effort was enough to reveal most lizard species occurring in the area; and richness estimators recovered values close to observed. For amphibians, the curve shows a weak tendency to stabilization with richness estimators indicating that additional records could be done. Field work carried out at PNSCo has highlighted an unique herpetofauna: five new species were described and there are three candidates as new species. The regional list including Cerrados’s units - Estação Ecológica Serra Geral do Tocantins (EESGT) and Estação Ecológica de Uruçuí-Una (EEUU) with Caatinga’s ones - PNSCo and Parque Nacional da Serra da Capivara (PNSCa), shows a high herpetofaunal diversity (191 species) to the region. The cluster analysis recovered the Cerrados’s units and Caatinga’s ones, in separate clusters evidencing a species turnover between domains, despite its geographical proximity. Thus, although there is widespread fauna throughout region shared by the units, each reserve holds its own faunal identity, harboring a singular assemblage of species. © 2016, Universidade Estadual de Campinas UNICAMP. All rights reserved.1632011/50206-9, FAPESP, Fundação de Amparo Pesquisa do Estado de São Paulo303545/2010-0, CNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico565046/2010-1, CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Immunoparasitological Diagnosis Of Strongyloides Stercoralis In Garbage Collectors In Uberlândia, Mg, Brazil

The objective of this study was to determine the presence of Strongyloides stercoralis in urban garbage collectors through the use of immunological and parasitological methods. A total of 92 individuals were evaluated from August, 1997, to June, 1998. For the parasitological diagnosis Baermann and Lutz' methods were applied. The immunological diagnosis involved the indirect fluorescence antibody test (IFAT) and the enzyme-linked immunosorbent assay (ELISA) to detect specific IgG antibodies. Of the 92 workers examined, six (6.5%) were infected with larvae of S. stercoralis. The IFAT detected 19 (16.3%) and the ELISA 17 (18.5%) positive serum samples. The differences between the results of parasitological and immunological methods were statistically significant (p<0.05). These results demonstrate that there is a need to improve the health conditions of this category of city employees.6203/04/15180182CONCHA, R., HARRINGTON, J.R.W., ROGERS, A.L., Intestinal strongyloidiasis: Recognition, management and determinants of outcome (2005) J Clin Gastroenterol, 39, pp. 203-211SIDDIQUI, A.A., BERK, S.L., Diagnosis of Strongyloides stercoralis infection (2001) Clin Infect Dis, 33, pp. 1040-1047FERREIRA, M., Strongyloidiasis and acquired immunodeficiency syndrome (2003) Enf Emerg, 5, pp. 18-26VADLAMUDI, R.S., CHI, D.S., KRISHANASWAMY, G., Intestinal strongyloidiasis and hyperinfection syndrome (2006) Clin Mol Allergy, 4, pp. 1-13BAERMANN G. Eine Einfache methode zur auffindung von Ankylostomum (Nematoden) larven in Erdproben. Mededeel mit h. Geneesk Lab Weltvreden Feestbundel, Batavia, 1917, p. 41-7LUTZ, A.V., Schistosoma mansoni e a schistosomose, Segundo observações feitas no Brasil. (1919) Mem Inst Oswaldo Cruz, 11, pp. 121-125COSTA-CRUZ, J.M., BULLAMAH, C.B., GONÇALVEZ-PIRES, M.R.F., Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis (1997) Rev Inst Med Trop São Paulo, 39, pp. 313-317MACHADO, E.R., UETA, M.T., GONÇALVES-PIRES, M.R.F., Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay (2003) Mem Inst Oswaldo Cruz, 98, pp. 849-853MACHADO, E.R., COSTA-CRUZ, J.M., Strongyloides stercoralis and other enteroparasites in children at Uberlândia City, state of Minas Gerais, Brazil (1998) Mem Inst Oswaldo Cruz, 93, pp. 161-164PAULA, F.M., CASTRO, E., GONÇALVES-PIRES, M.R.F., Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and nonimmunocompromised children at Uberlândia City, state of Minas Gerais, Brazil (2000) Rev Inst Med Trop São Paulo, 42, pp. 51-55OLIVEIRA, L.C.M., RIBEIRO, C.T., MENDES, D.M., Frequency of Strongyloides stercoralis in alcoholics (2002) Mem Inst Oswaldo Cruz, 97, pp. 119-121LINDO, J.F., CONWAY, D.J., ATKINS, N.S., Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection (1994) Am J Trop Med Hyg, 51, pp. 175-179CLARK, C.S., LINNEMANN Jr, C.C., CLARK, J.G., Enteric parasites in workers occupationally exposed to sewage (1984) J Occup Med, 26, pp. 273-275SCHLOSSER, O., RALL, D., LAURECEAU, M.-N., Intestinal parasite carriage in workers exposed to sewage (1999) Eur J Epidemiol, 15, pp. 261-265GOMES, T.C., ALMEIDA, M.F., MUIRA, L.A., Helmintoses intestinais em população de rua da cidade do Rio de Janeiro. (2002) Rev Soc Bras Med Trop, 35, pp. 531-53

Phenotypic and genetic heterogeneity of lactic acid bacteria isolated from “Alheira”, a traditional fermented sausage produced in Portugal

The aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (LAB) isolated from ‘‘alheira”, a fermented sausage produced in Portugal. LAB were identified to genus and species level by phenotypic characteristics, using genus or speciesspecific primers and sequencing of the gene encoding 16S rRNA. Two-hundred and eighty-three isolates were grouped into 14 species. Lactobacillus plantarum was isolated from all sausages and Enterococcus faecalis from most of the samples. Low numbers of Lactobacillus paraplantarum, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus sakei, Lactobacillus zeae, Lactobacillus paracasei, Leuconostoc mesenteroides, Pediococcus pentosaceus, Pediococcus acidilactici, Weissella cibaria, Weissella viridescens and Enterococcus faecium were recorded. The genetic heterogeneity of L. plantarum and E. faecalis strains were determined by numerical analysis of DNA banding patterns obtained by RAPD–PCR. Strains of L. plantarum and E. faecalis were different from different producers. This study forms the basis from which starter cultures could be selected for production of ‘‘Alheira”.info:eu-repo/semantics/acceptedVersio

Adaptation to DNA damage checkpoint in senescent telomerase-negative cells promotes genome instability.

In cells lacking telomerase, telomeres gradually shorten during each cell division to reach a critically short length, permanently activate the DNA damage checkpoint, and trigger replicative senescence. The increase in genome instability that occurs as a consequence may contribute to the early steps of tumorigenesis. However, because of the low frequency of mutations and the heterogeneity of telomere-induced senescence, the timing and mechanisms of genome instability increase remain elusive. Here, to capture early mutation events during replicative senescence, we used a combined microfluidic-based approach and live-cell imaging in yeast. We analyzed DNA damage checkpoint activation in consecutive cell divisions of individual cell lineages in telomerase-negative yeast cells and observed that prolonged checkpoint arrests occurred frequently in telomerase-negative lineages. Cells relied on the adaptation to the DNA damage pathway to bypass the prolonged checkpoint arrests, allowing further cell divisions despite the presence of unrepaired DNA damage. We demonstrate that the adaptation pathway is a major contributor to the genome instability induced during replicative senescence. Therefore, adaptation plays a critical role in shaping the dynamics of genome instability during replicative senescence

Caracterização estato-ponderal de crianças e adolescentes numa amostra representativa da população portuguesa

[resumo][abstract

Avaliação estato-ponderal e da actividade física numa amostra representativa da população adulta portuguesa

[resumo][abstract

Determinantes da saúde e escolha alimentar em idosos portugueses

[resumo][abstract

Avaliação do aporte energético e nutricional e sua relação com dados antropométricos dos idosos portugueses

[resumo][abstract