Risk factors for chronic non communicable diseases in Mombasa, Kenya: Epidemiological study using WHO stepwise approach

Objective: To describe the prevalence and distribution patterns of the major common risk factors for non communicable diseases among the people living in Mombasa, Kenya. Methods: Using the WHO STEPwise approach, risk factors for non communicable diseases were analyzed for 305 people aged between 13 to 67 years. The study sample was arrived at through convenient stratification of the population according to age and setting followed by random selection of the participants. Results: The most common individual risk factors registered were physical inactivity, hypertension and overweight/obesity accounting for 42%, 24% and 11% of the sample respectively. Participants who possessed a single risk factor profile were 42% and those who had multiple risk factors were approximately 17%. Hypertension and physical inactivity were the most common multiple risk factor pattern possessed by 7.5% of the participants who had at least one of the investigated risk factors for CNCDs. Socio-demographic characteristics including male gender, increasing age, being a student and low socio-economic status were found to be positive predictors of CNCDs Conclusion: The burden of CNCDs risk factors is unequally distributed among Mombasa residents. The poorest quintile posses the worst risk factor profile compared to their privileged counterparts. The implementation of WHO STEPwise approach was feasible since it revealed a comprehensive picture of the at-risk groups thus forming a vital baseline framework for target-specific and cost-effective CNCDs control and prevention interventions. Keywords: Chronic non communicable diseases, Risk factors, Health promotion, Epidemiology, Mombas

X-ray Temperature and Mass Measurements to the Virial Radius of Abell 1413 with Suzaku

We present X-ray observations of the northern outskirts of the relaxed galaxy cluster A1413 with Suzaku, whose XIS instrument has the low intrinsic background needed to make measurements of these low surface brightness regions. We excise 15 point sources superimposed on the image above a flux of $1\times 10^{-14}$ \fluxunit (2--10keV) using XMM-Newton and Suzaku images of the cluster. We quantify all known systematic errors as part of our analysis, and show our statistical errors encompasses them for the most part. Our results extend previous measurements with Chandra and XMM-Newton, and show a significant temperature drop to about 3keV at the virial radius, $r_{200}$. Our entropy profile in the outer region ($> 0.5 r_{200}$) joins smoothly onto that of XMM-Newton, and shows a flatter slope compared with simple models, similar to a few other clusters observed at the virial radius. The integrated mass of the cluster at the virial radius is approximately $7.5\times10^{14}M_{\odot}$ and varies by about 30% depending on the particular method used to measure it.Comment: 32pages, 9 figures, accepted for publication in PAS

X-ray and UV spectroscopy of Galactic diffuse hot gas along the LMC X--3 sight line

We present Suzaku spectra of X-ray emission in the fields just off the LMC X-3 sight line. OVII, OVIII, and NeIX emission lines are clearly detected, suggesting the presence of an optically thin thermal plasma with an average temperature of 2.4E6. This temperature is significantly higher than that inferred from existing X-ray absorption line data obtained with Chandra grating observations of LMC X-3, strongly suggesting that the gas is not isothermal. We then jointly analyze these data to characterize the spatial and temperature distributions of the gas. Assuming a vertical exponential Galactic disk model, we estimate the gas temperature and density at the Galactic plane and their scale heights as 3.6(2.9, 4.7)E6 K and 1.4(0.3, 3.4)E-3 cm^{-3} and 1.4(0.2, 5.2) kpc and $2.8(1.0, 6.4)$ kpc, respectively. This characterization can account for all the \ovi line absorption, as observed in a FUSE spectrum of LMC X-3, but only predicts less than one tenth of the OVI line emission intensity typically detected at high Galactic latitudes. The bulk of the OVI emission most likely arises at interfaces between cool and hot gases.Comment: 10 pages, 7 figures, 3 tables, accepted for publication in ApJ, 200

On the lack of strong O-line excess in the Coma cluster outskirts from Suzaku

About half of the baryons in the local Universe are thought to reside in the so-called warm-hot intergalactic medium (WHIM) at temperatures of 0.1-10 million K. Thermal soft excess emission in the spectrum of some cluster outskirts that contains OVII and/or OVIII emission lines is regarded as evidence of the WHIM, although the origin of the lines is controversial due to strong Galactic and solar system foreground emission. We observed the Coma-11 field, where the most prominent thermal soft excess has ever been reported, with Suzaku XIS in order to make clear the origin of the excess. We did not confirm OVII or OVIII excess emission. The OVII and OVIII intensity in Coma-11 is more than 5 sigma below that reported before and we obtained 2 sigma upper limits of 2.8 and 2.9 photons cm^-2 s^-1 sr^-1 for OVII and OVIII, respectively. The intensities are consistent with those in another field (Coma-7) that we measured, and with other measurements in the Coma outskirts (Coma-7 and X Com fields with XMM-Newton). We did not confirm the spatial variation within Coma outskirts. The strong oxygen emission lines previously reported are likely due to solar wind charge exchange.Comment: 5 pages, 3 figures. Accepted for publication in Ap

Suzaku measurement of Abell 2204's intracluster gas temperature profile out to 1800 kpc

Context: Measurements of intracluster gas temperatures out to large radii are important for the use of clusters for precision cosmology and for studies of cluster physics. Previous attempts to measure robust temperatures at cluster virial radii failed. Aims: The goal of this work is to measure the temperature profile of the very relaxed galaxy cluster Abell 2204 out to large radii, possibly reaching the virial radius. Methods: Taking advantage of its low particle background due to its low-Earth orbit, Suzaku data are used to measure the outer temperature profile of Abell 2204. These data are combined with Chandra and XMM-Newton data of the same cluster in order to make the connection to the inner regions, unresolved by Suzaku, and to determine the smearing due to Suzaku's PSF. Results: The temperature profile of Abell 2204 is determined from 10 kpc to 1800 kpc, close to an estimate of r200 (the approximation to the virial radius). The temperature rises steeply from below 4 keV in the very center up to more than 8 keV in the intermediate range and then decreases again to about 4 keV at the largest radii. Varying the measured particle background normalization artificially by +-10 percent does not change the results significantly. Predictions for outer temperature profiles based on hydrodynamic simulations show good agreement. In particular, we find the observed temperature profile to be slightly steeper but consistent with a drop of a factor of 0.6 from 0.3 r200 to r200, as predicted by simulations. Conclusions: Temperature measurements up to the virial radius seem feasible with Suzaku, when a careful analysis of the different background components and the effects of the PSF is performed. The result obtained here indicates that numerical simulations capture the intracluster gas physics well in cluster outskirts.Comment: 7 pages; Astronomy and Astrophysics, accepted; additional systematic effects have been quantified, results unchanged; also available at http://www.reiprich.ne

Exceeding the Limits of Static Cold Storage in Limb Transplantation Using Subnormothermic Machine Perfusion

Background For 50 years, static cold storage (SCS) has been the gold standard for solid organ preservation in transplantation. Although logistically convenient, this preservation method presents important constraints in terms of duration and cold ischemia-induced lesions. We aimed to develop a machine perfusion (MP) protocol for recovery of vascularized composite allografts (VCA) after static cold preservation and determine its effects in a rat limb transplantation model. Methods Partial hindlimbs were procured from Lewis rats and subjected to SCS in Histidine-Tryptophan-Ketoglutarate solution for 0, 12, 18, 24, and 48 hours. They were then either transplanted (Txp), subjected to subnormothermic machine perfusion (SNMP) for 3 hours with a modified Steen solution, or to SNMP + Txp. Perfusion parameters were assessed for blood gas and electrolytes measurement, and flow rate and arterial pressures were monitored continuously. Histology was assessed at the end of perfusion. For select SCS durations, graft survival and clinical outcomes after transplantation were compared between groups at 21 days. Results Transplantation of limbs preserved for 0, 12, 18, and 24-hour SCS resulted in similar survival rates at postoperative day 21. Grafts cold-stored for 48 hours presented delayed graft failure (p = 0.0032). SNMP of limbs after 12-hour SCS recovered the vascular resistance, potassium, and lactate levels to values similar to limbs that were not subjected to SCS. However, 18-hour SCS grafts developed significant edema during SNMP recovery. Transplantation of grafts that had undergone a mixed preservation method (12-hour SCS + SNMP + Txp) resulted in better clinical outcomes based on skin clinical scores at day 21 post-transplantation when compared to the SCS + Txp group (p = 0.01613). Conclusion To date, VCA MP is still limited to animal models and no protocols are yet developed for graft recovery. Our study suggests that ex vivo SNMP could help increase the preservation duration and limit cold ischemia-induced injury in VCA transplantation.</p

Monte-Carlo Simulator and Ancillary Response Generator of Suzaku XRT/XIS System for Spatially Extended Source Analysis

We have developed a framework for the Monte-Carlo simulation of the X-Ray Telescopes (XRT) and the X-ray Imaging Spectrometers (XIS) onboard Suzaku, mainly for the scientific analysis of spatially and spectroscopically complex celestial sources. A photon-by-photon instrumental simulator is built on the ANL platform, which has been successfully used in ASCA data analysis. The simulator has a modular structure, in which the XRT simulation is based on a ray-tracing library, while the XIS simulation utilizes a spectral "Redistribution Matrix File" (RMF), generated separately by other tools. Instrumental characteristics and calibration results, e.g., XRT geometry, reflectivity, mutual alignments, thermal shield transmission, build-up of the contamination on the XIS optical blocking filters (OBF), are incorporated as completely as possible. Most of this information is available in the form of the FITS (Flexible Image Transport System) files in the standard calibration database (CALDB). This simulator can also be utilized to generate an "Ancillary Response File" (ARF), which describes the XRT response and the amount of OBF contamination. The ARF is dependent on the spatial distribution of the celestial target and the photon accumulation region on the detector, as well as observing conditions such as the observation date and satellite attitude. We describe principles of the simulator and the ARF generator, and demonstrate their performance in comparison with in-flight data.Comment: 19 pages with 8 figures, accepted for publication in PASJ Vol 58, Suzaku special issu

Towards a target label-free suboptimum oligonucleotide displacement-based detection system

A novel method for the future development of label-free DNA sensors is proposed here. The approach is based on the displacement of a labelled suboptimum mutated oligonucleotide hybridised with the immobilised biotin-capture probe. The target fully complementary to the biotin-capture probe can displace the labelled oligonucleotide causing a subsequent decrease of the signal that verifies the presence of the target. The decrease of signal was demonstrated to be proportional to the target concentration. A study of the hybridisation of mutated and complementary labelled oligonucleotides with an immobilised biotin-capture probe was carried out. Different kinetic and thermodynamic behaviour was observed for heterogeneous hybridisation of biotin-capture probe with complementary or suboptimum oligonucleotides. The displacement method evaluated colourimetrically achieved the objective of decreasing the response time from 1 h for direct hybridisation of 19-mer oligonucleotides in the direct enzyme-linked oligonucleotide assay (ELONA) to 5 min in the case of displacement detection in the micromolar concentration range

Muscle wasting in chronic kidney disease: the role of the ubiquitin proteasome system and its clinical impact

Muscle wasting in chronic kidney disease (CKD) and other catabolic diseases (e.g. sepsis, diabetes, cancer) can occur despite adequate nutritional intake. It is now known that complications of these various disorders, including acidosis, insulin resistance, inflammation, and increased glucocorticoid and angiotensin II production, all activate the ubiquitin–proteasome system (UPS) to degrade muscle proteins. The initial step in this process is activation of caspase-3 to cleave the myofibril into its components (actin, myosin, troponin, and tropomyosin). Caspase-3 is required because the UPS minimally degrades the myofibril but rapidly degrades its component proteins. Caspase-3 activity is easily detected because it leaves a characteristic 14kD actin fragment in muscle samples. Preliminary evidence from several experimental models of catabolic diseases, as well as from studies in patients, indicates that this fragment could be a useful biomarker because it correlates well with the degree of muscle degradation in dialysis patients and in other catabolic conditions

Racism as a determinant of health: a systematic review and meta-analysis

Despite a growing body of epidemiological evidence in recent years documenting the health impacts of racism, the cumulative evidence base has yet to be synthesized in a comprehensive meta-analysis focused specifically on racism as a determinant of health. This meta-analysis reviewed the literature focusing on the relationship between reported racism and mental and physical health outcomes. Data from 293 studies reported in 333 articles published between 1983 and 2013, and conducted predominately in the U.S., were analysed using random effects models and mean weighted effect sizes. Racism was associated with poorer mental health (negative mental health: r = -.23, 95% CI [-.24,-.21], k = 227; positive mental health: r = -.13, 95% CI [-.16,-.10], k = 113), including depression, anxiety, psychological stress and various other outcomes. Racism was also associated with poorer general health (r = -.13 (95% CI [-.18,-.09], k = 30), and poorer physical health (r = -.09, 95% CI [-.12,-.06], k = 50). Moderation effects were found for some outcomes with regard to study and exposure characteristics. Effect sizes of racism on mental health were stronger in cross-sectional compared with longitudinal data and in non-representative samples compared with representative samples. Age, sex, birthplace and education level did not moderate the effects of racism on health. Ethnicity significantly moderated the effect of racism on negative mental health and physical health: the association between racism and negative mental health was significantly stronger for Asian American and Latino(a) American participants compared with African American participants, and the association between racism and physical health was significantly stronger for Latino(a) American participants compared with African American participants.<br /