Measuring the Sustainability of Cities: A Survey-Based Analysis of the Use of Local Indicators

We analyze 17 studies of the use of sustainable development indicators (SDI) in an urban setting. The analysis reveals a lack of consensus not only on the conceptual framework and the approach favored, but also on the selection and optimal number of indicators. First, by performing different classifications and categorizations of SDI we identify problems inherent in territorial practices that use SDI. Second, we argue that the lack of consensus in several steps of the creation of SDI stems notably from the ambiguity in the definitions of sustainable development, objectives for the use of such indicators, the selection method and the accessibility of qualitative and quantitative data. Third, we propose a selection strategy for SDI through which we demonstrate the need to adopt a parsimonious list of SDI covering the sustainable development components and their constituent categories as broadly as possible while minimizing the number of indicators retained. Nous analysons 17 études traitant de l’utilisation d’indicateurs de développement durable (IDD) en milieu urbain pour différents pays, provinces ou états occidentaux. 188 IDD différents sont recensés dans ces études dont 135 (72 %) ne sont utilisés qu’une ou deux fois. L’analyse de ces études révèle ainsi un faible consensus non seulement au niveau du cadre conceptuel ou de l’approche préconisée, mais aussi en ce qui concerne la sélection et le nombre d’indicateurs optimal. Premièrement, différents classements et catégorisations des IDD recensés nous permettent d’observer et d’identifier les problèmes inhérents aux pratiques territoriales ayant recours aux IDD. Deuxièmement, nous argumentons que l’absence de consensus à plusieurs étapes de la création des IDD émergent entre autres de l’ambiguïté occasionnée par la définition du développement durable, des objectifs visées par l’utilisation de tels indicateurs, de la méthode de sélection préconisée et de l’accessibilité des données qualitatives et quantitatives en cette matière. Troisièmement, nous proposons une stratégie de sélection des IDD (que nous appelons SuBSeleC) où nous démontrons la nécessité d’adoption d’une liste parcimonieuse d’IDD couvrant le plus largement possible les volets du développement durable et des catégories qui les composent tout en minimisant le nombre d’indicateurs retenus. Le résultat est une liste concise et moins redondante d’indicateurs moins sectoriels et plus intégrateurs ayant l’avantage d’englober les dimensions intégrées du développement durable.Cities, Indicators, Sustainable Development, Environment, Local Governance., Villes, indicateurs, développement durable, environnement, gouvernance locale.

A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay

BACKGROUND: Coupling of alternative splicing with nonsense-mediated mRNA decay (NMD) may regulate gene expression. We report here the identification of a nonsense alternative transcript of the fumarylacetoacetate hydrolase (fah) gene, which produces a protein despite the fact that it is subject to NMD. RESULTS: During the characterization of the effects of the W262X nonsense mutation on FAH mRNA metabolism, two alternative transcripts (del100 and del231) of the fah gene were identified. Del100 lacks exon 8 and as a consequence, the reading frame is shifted and a premature termination codon appears at the 3'end of exon 10. Exons 8 and 9 are skipped in del231, without any disruption of the reading frame. Specific amplification of these transcripts demonstrate that they are produced through minor alternative splicing pathways, and that they are not caused by the W262X mutation per se. As shown with an antiserum raised against the C-terminal part of the putative DEL100 protein, the del100 transcript produces a protein, expressed at different levels in various human tissues. Interestingly, the del100 transcript seems to be subjected to nonsense-mediated mRNA decay, as its level was stabilized following a cycloheximide treatment. CONCLUSIONS: The del100 and del231 transcripts arise due to minor alternative splicing pathways and del100 is likely subjected to nonsense-mediated mRNA decay. However the remaining amount of transcript seems sufficient to produce a protein in different human tissues. This suggests that NMD has a broader role than simply eliminating aberrant transcripts and when coupled to alternative splicing, may act to modulate gene expression, by allowing the production of low amounts of protein

Nature of fatty acids in high fat diets differentially delineates obesity-linked metabolic syndrome components in male and female C57BL/6J mice

<p>Abstract</p> <p>Background</p> <p>Adverse effects of high-fat diets (HFD) on metabolic homeostasis are linked to adipose tissue dysfunction. The goal of this study was to examine the effect of the HFD nature on adipose tissue activity, metabolic disturbances and glucose homeostasis alterations in male mice compared with female mice.</p> <p>Methods</p> <p>C57BL/6J mice were fed either a chow diet or HFD including vegetal (VD) or animal (AD) fat. Body weight, plasmatic parameters and adipose tissue mRNA expression levels of key genes were evaluated after 20 weeks of HFD feeding.</p> <p>Results</p> <p>HFD-fed mice were significantly heavier than control at the end of the protocol. Greater abdominal visceral fat accumulation was observed in mice fed with AD compared to those fed a chow diet or VD. Correlated with weight gain, leptin levels in systemic circulation were increased in HFD-fed mice in both sexes with a significant higher level in AD group compared to VD group. Circulating adiponectin levels as well as adipose tissue mRNA expression levels were significantly decreased in HFD-fed male mice. Although its plasma levels remained unchanged in females, adiponectin mRNA levels were significantly reduced in adipose tissue of both HFD-fed groups with a more marked decrease in AD group compared to VD group. Only HFD-fed male mice were diabetic with increased fasting glycaemia. On the other hand, insulin levels were only increased in AD-fed group in both sexes associated with increased resistin levels. VD did not induce any apparent metabolic alteration in females despite the increased weight gain. Peroxisome Proliferator-Activated Receptors gamma-2 (PPARγ2) and estrogen receptor alpha (ERα) mRNA expression levels in adipose tissue were decreased up to 70% in HFD-fed mice but were more markedly reduced in male mice as compared with female mice.</p> <p>Conclusions</p> <p>The nature of dietary fat determines the extent of metabolic alterations reflected in adipocytes through modifications in the pattern of adipokines secretion and modulation of key genes mRNA expression. Compared with males, female mice demonstrate higher capacity in controlling glucose homeostasis in response to 20 weeks HFD feeding. Our data suggest gender specific interactions between the diet's fatty acid source, the adipocyte-secreted proteins and metabolic disorders.</p

Profil de trois enquêtes nationales sur la pratique pharmaceutique hospitalière

RésuméObjectif : L’objectif de cet article est de situer l’enquête canadienne sur la pharmacie hospitalière par rapport aux enquêtes européennes et américaines.Description de la problématique : Les chefs de départements de pharmacie et les pharmaciens sont peu exposés aux résultats des enquêtes américaine et européenne sur la pratique pharmaceutique hospitalière.Discussion : Afin de situer l’enquête canadienne sur la pharmacie hospitalière par rapport aux enquêtes menées en Europe et aux États-Unis, nous avons consulté les plus récentes versions des trois enquêtes et comparé leurs méthodologies et leurs thèmes. Les trois enquêtes existent depuis plus de 20 ans et sont menées auprès des chefs de départements de pharmacie. Elles explorent les thèmes des ressources humaines, de la distribution et de la préparation des médicaments, du bon usage des médicaments, de la décentralisation des pharmaciens dans les programmes de soins, des activités pharmaceutiques, des technologies et des pratiques de soins sans danger. Le taux de participation à l’enquête canadienne est supérieur à celui des deux autres enquêtes. Alors que l’enquête canadienne présente les données en parallèle des données précédentes, l’enquête américaine comporte un historique complet des tendances.Conclusion : Les chefs de départements de pharmacie devraient consulter périodiquement ces enquêtes afin d’assurer un développement cohérent de leur pratique.AbstractObjective: The objective of this article is to situate the Canadian Hospital Pharmacy Survey in relation to the European and American surveys.Description of the problem: Directors of Department of pharmacy and pharmacists are not aware of the results of the American and European surveys on hospital pharmaceutical practice.Discussion: To situate the Canadian Hospital Pharmacy Survey in relation to the surveys conducted in Europe and the United States, we consulted the latest versions of the three surveys and compared their methodologies and themes. All three surveys, conducted among directors of pharmacy departments, have been in existence for more than 20 years. They explore the following themes: human resources, medication preparation and distribution, proper medication use, decentralization of pharmacists in care programs, pharmaceutical activities, technologies, and safe care practices. The participation rate in the Canadian survey is higher than in the other two. While the Canadian survey compares data with those from the previous survey, the American survey provides a complete trend history.Conclusion: Directors of Department of pharmacy should consult these surveys periodically to ensure that their practice develops in a consistent manner

Dual Antiplatelet Therapy after PCI: When Could We Go Shorter?

The optimal duration of dual antiplatelet therapy (DAPT) after percutaneous coronary intervention (PCI) remains an important clinical question in interventional cardiology. Several clinical and angiographic variables are associated with an increased risk for thrombotic events, and prolonged DAPT duration may improve long term clinical outcome. However, some patients also present high bleeding risk (HBR) characteristics and may require a shorter DAPT duration. The guidelines recommendations consider the data from randomized clinical trials, however numerous exclusion criteria may create gaps in the evidence leading to uncertainties, the need for expert opinion and patient level decision making. Furthermore, the stent platforms have evolved in such way that opportunities now exist to shorten duration of DAPT. This chapter will review the variables associated with ischemic and bleeding risks as well as different stent platforms to help clinicians optimize DAPT duration in patients undergoing PCI

Multifunctional surface modifications to capture and expand flowing endothelial colony- forming cells

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Head‐to‐Head comparison of consensus‐recommended platelet function tests to assess P2Y12 Inhibition : insights for multi‐center trials

The vasodilator‐associated stimulated phosphoprotein (VASP) phosphorylation level is a highly specific method to assess P2Y12 receptor inhibition. Traditionally, VASP phosphorylation is analyzed by flow cytometry, which is laborious and restricted to specialized laboratories. Recently, a simple ELISA kit has been commercialized. The primary objective of this study was to compare the performance of VASP assessment by ELISA and flow cytometry in relation to functional platelet aggregation testing by Multiplate® whole‐blood aggregometry. Blood from 24 healthy volunteers was incubated with increasing concentration of a P2Y12 receptor inhibitor (AR‐C 66096). Platelet function testing was carried out simultaneously by Multiplate® aggregometry and by VASP assessment through ELISA and flow cytometry. As expected, increasing concentrations of the P2Y12 receptor inhibitor induced a proportional inhibition of platelet aggregation and P2Y12 receptor activation across the modalities. Platelet reactivity index values of both ELISA‐ and flow cytometry‐ based VASP assessment methods correlated strongly (r = 0.87, p < 0.0001) and showed minimal bias (1.05%). Correlation with Multiplate® was slightly higher for the flow cytometry‐based VASP assay (r = 0.79, p < 0.0001) than for the ELISA‐based assay (r = 0.69, p < 0.0001). Intraclass correlation (ICC) was moderate for all the assays tested (ICC between 0.62 and 0.84). However, categorization into low, optimal, or high platelet reactivity based on these assays was strongly concordant (κ between 0.86 and 0.92). In conclusion, the consensus‐recommended assays with their standardized cut‐offs should not be used interchangeably in multi‐center clinical studies but, rather, they should be standardized throughout sites

Reversal of Cardiac Dysfunction After Long-Term Expression of SERCA2a by Gene Transfer in a Pre-Clinical Model of Heart Failure

ObjectivesThe aim of this study was to examine the effects of sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) gene transfer in a swine heart failure (HF) model.BackgroundReduced expression and activity of SERCA2a have been documented in HF. Prior studies have reported the beneficial effects of short-term SERCA2a overexpression in rodent models. However, the effects of long-term expression of SERCA2a in pre-clinical large animal models are not known.MethodsYorkshire-Landrace pigs were used (n = 16) to create volume overload by percutaneously severing chordae tendinae of the mitral apparatus with a bioptome to induce mitral regurgitation. At 2 months, pigs underwent intracoronary delivery of either recombinant adeno-associated virus type 1 (rAAV1) carrying SERCA2a under a cytomegalovirus promoter (rAAV1.SERCA2a) (n = 10; group 1) or saline (n = 6; group 2).ResultsAt 2 months, study animals were found to be in a compensated state of volume-overload HF (increased left ventricular internal diastolic and systolic diameters [LVIDd and LVIDs]). At 4 months, gene transfer resulted in: 1) positive left ventricular (LV) inotropic effects (adjusted peak left ventricular pressure rate of rise (dP/dt)max/P, 21.2 ± 3.2 s−1 group 1 vs. 15.5 ± 3.0 s−1 group 2; p < 0.01); 2) improvement in LV remodeling (% change in LVIDs −3.0 ± 10% vs. +15 ± 11%, respectively; p < 0.01). At follow-up, brain natriuretic peptide levels remained stable in group 1 after gene transfer, in contrast to rising levels in group 2. Further, cardiac SERCA2a expression was significantly decreased in group 2 whereas in group 1 it was restored to normal levels. There was no histopathological evidence of acute myocardial inflammation or necrosis.ConclusionsUsing a large-animal, volume-overload model of HF, we report that long-term overexpression of SERCA2a by in vivo rAAV1-mediated intracoronary gene transfer preserved systolic function, potentially prevented diastolic dysfunction, and improved ventricular remodeling