Efecto del grado de hidrólisis sobre las propiedades funcionales de proteínas de semillas oleaginosas

Hydrolyzing soybean, sesame, and ricebran meals with papain and bromelain enzymes under previously determined optimum conditions was accomplished. Aliquots were withdrawn from the bulk hydrolysates at different time intervals to determine the rate of enzymatic degradation,as well as the degree of hydrolysis of the hydrolysates at different time intervals. Twelve hydrolysates were then chosen with low and high degree of hydrolysis. They also represented hydrolysates from the three substrates hydrolysed with the two enzymes used. Some functional properties of the twelve hydrolysates were examined and then an attempt to correlate the functional properties with the degree of hydrolysis was done. The investigated functional properties included: wettability, dispersibility, flowability, bulk density,nitrogen solubility index,water absorption capacity, oil holding capacity, gelation, thermostability, emulsifying capacity and foam stability.Results showed a direct relation between increasing degree of hydrolysis and nitrogen solubility and dispersibility. Wettability, flowability and emulsifying capacity gave better results with low degree of hydrolysis.Se ha efectuado la hidrólisis de harinas de haba de soja, sésamo y germen de arroz con las enzimas papaina y bromelaina bajo condiciones óptimas previamente determinadas. Se sacaron alicuotas de los hidrolizados a diferentes intervalos de tiempo para determinar la velocidad de degradación enzimática, así como el grado de hidrólisis de los hidrolizados. Con estos datos se eligieron doce hidrolizados con bajo y alto grado de hidrólisis, que fueron representativos de los tres sustratos y los dos enzimas ensayados. Se examinaron algunas propiedades funcionales de estos doce hidrolizados y luego se llevó a cabo un intento de correlacionarlas con el grado de hidrólisis. Las propiedades funcionales investigadas incluyeron: humectabilidad, dispersabilidad, fluidez, densidad volumétrica, índice de solubilidad del nitrógeno, capacidad de absorción de agua, capacidad de retención de aceite, gelación, termoestabilidad, capacidad emulsionante y estabilidad espumante. Los resultados mostraron una relación directa entre el aumento del grado de hidrólisis y la solubilidad del nitrogéno y la dispersabilidad. La humectabilidad, la fluidez y la capacidad emulsionante dieron mejores resultados con un grado de hidrólisis bajo

Protective Effect of Humic acid and Chitosan on Radish (Raphanus sativus, L. var. sativus) Plants Subjected to Cadmium Stress

BackgroundHumic acid or chitosan has been shown to increase plant growth, yield and improving physiological processes in plant, but its roles on alleviating the harmful effect of cadmium on plant growth and some physiological processes in plants is very rare. Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth and some physiological charactersResultsCadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons. Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased. Meanwhile, cadmium concentration in plants was increased. On the other hand, application of chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1 increased all the above mentioned parameters and decreased cadmium concentrations in plant tissues. Chitosan at 200 mg kg-1 was the most effective than humic acid at both concentrations in counteracting the harmful effect of cadmium stress on radish plant growth.ConclusionIn conclusion, both natural chelators, in particular, chitosan at 200 mg/kg dry soil can increase the capacity of radish plant to survive under cadmium stress due to chelating the Cd in the soil, and then reduced Cd bio-availability

Estimation of Water and Energy Saving by Rainwater Harvesting: Sulaimani City as a Case Study

Rainwater harvesting could be a possible solution to decrease the consequences of water scarcity and energy deficiency in Iraq and the Kurdistan Region of Iraq (KRI). This study aims to calculate the water and energy (electricity) saved by rainwater harvesting for rooftops and green areas in Sulaimani city, KR, Iraq. Various data were acquired from different formal entities in Sulaimani city. Moreover, Google Earth and ArcMap 10.4 software were used for digitizing and calculating the total rooftop and green areas. The results showed that for the used runoff coefficients (0.8 and 0.95), the harvested rainwater volumes were 2901563 and 12197131 m³ during the study period (2005 – 2006) and (2019-2020). Moreover, by comparing the study area's rainwater harvesting volume and water production, the water-saving percentage was 8.21 to 22.68%. Furthermore, the energy-saving percentage recorded was from 7.70 to 22.5% by implementing rooftop rainwater harvesting. On the other hand, using average daily rainfall data for the year (2005-2020), the total water-saving percentage and the total energy-saving rate for both runoff coefficients were very close. Water and energy-saving results were calculated using year-by-year rainfall data, taking more time and effort for its computation. Moreover, the water-saving percentage for the selected green area was not encouraging, and the results were between 0.73 and 11.15%. Additionally, the storage size for three typical buildings was calculated, and the results show the average storage size required for rainwater harvesting using daily rainfall data was 11.2 to 14.68 m³ (house), 291.42 to 422.33 m³ (school), and 10.5 to 11.41 m³ (hotel) for runoff coefficients of 0.8 and 0.95, respectively.

فاعلية نظم المعلومات المحاسبية في ضوء متطلبات الحوكمة الرقمية: دراسة تحليلية

هدف البحث الكشف عن تأثير متطلبات الحوكمة الرقمية في تعزيز فاعلية نظم المعلومات المحاسبية في المصارف التجارية الخاصة العراقية. اذ يقدم البحث الحالي نموذج مفاهيمي مقترح يجادل بأن الامتثال للقوانين والتعليمات، والخصوصية، والأمان، والشفافية هي مكونات أساسية للحوكمة الرقمية، والتي تؤثر بشكل إيجابي ومعنوي على فاعلية أنظمة المعلومات المحاسبية. تم تصميم نموذج القياس والهيكلي باستخدام أسلوب نمذجة المعادلات الهيكلية المستند الى طريقة المربعات الجزئية او الصغرى، كما تم توظيف برمجية (SmartPLSv.3.9.6) كأداة احصائية. تم اختبار فرضية رئيسة واحدة وأربعة أخرى فرعية، وذلك باستخدام دراسة استقصائية شملت (121) محاسباً ومدققاً في (45) مصرفاً خاصاً في العراق. ووجد المؤلفون أدلة تجريبية تدعم حججهم. تظهر النتائج أنه كلما زادت امتثال المصارف لمتطلبات الحوكمة الرقمية، زادت فاعلية نظم المعلومات المحاسبية لديها. تقدم الدراسة رؤى للمديرين والمحاسبين والمدققين وواضعي السياسات حول أهمية متطلبات الحوكمة الرقمية في تعزيز فاعلية نظم المعلومات المحاسبية، وخاصة في القطاع المصرفي

Implementation of variational iteration method for various types of linear and nonlinear partial differential equations

There are various linear and nonlinear one-dimensional partial differential equations that are the focus of this research. There are a large number of these equations that cannot be solved analytically or precisely. The evaluation of nonlinear partial differential equations, even if analytical solutions exist, may be problematic. Therefore, it may be necessary to use approximate analytical methodologies to solve these issues. As a result, a more effective and accurate approach must be investigated and analyzed. It is shown in this study that the Lagrange multiplier may be used to get an ideal value for parameters in a functional form and then used to construct an iterative series solution. Linear and nonlinear partial differential equations may both be solved using the variational iteration method (VIM) method, thanks to its high computing power and high efficiency. Decoding and analyzing possible Korteweg-De-Vries, Benjamin, and Airy equations demonstrates the method’s ability. With just a few iterations, the produced findings are very effective, precise, and convergent to the exact answer. As a result, solving nonlinear equations using VIM is regarded as a viable option

Nodulation and nitrogen fixation of some wild legumes from differing habitats in Egypt

This study was devoted to exploring the natural nodulation and nitrogen fixation of wild legumes grown in different Egyptian habitats. These habitats are representative to four phytogeographical regions. Sites that inhabited by Melilotus indicus, Medicago polymorpha, Trifolium resupinatum, Trigonella hamosa and Vicia sativa in each region were selected for study. High nodulation, nitrogen fixation and plant biomass were recorded in plants grown at Nile region and Oases compared with those at Mediterranean region and Sinai. The inhibition in nodulation and potential of nitrogen fixation in legumes at MR and S were attributed to drought and low soil fertility. Differences in species, regions or their interaction have significant effect on nodulation, legheamoglobin, nitroginase activity and biomass of nodules, shoots and roots; the magnitude of effect due to different species was the greatest. Five rhizobial isolates (Sinorhizobium fredii, Rhizobium mesosinicum, Rhizobium daejeonense, Rhizobium huautlense, Rhizobium alamii) recovered from root nodules of the five species were identified by 16S rRNA gene sequence. The indigenous rhizobia of legumes grown at MR and S expected to be exhibit higher tolerance to the existing harsh environmental conditions. These rhizobia can be used as inoculants for crop legumes under unfavorable environmental conditions of agroecosystems or recently reclaimed desert. DOI: http://dx.doi.org/10.5281/zenodo.22401

Hybrid Grey Wolf Optimizer for Transformer Fault Diagnosis Using Dissolved Gases Considering Uncertainty in Measurements

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Condiciones óptimas para la degradación enzimática de proteínas de semillas oleaginosas

Soybean, sesame seed, and rice bran meal proteins were hydrolyzed with two enzymes, namely, papain and bromelain. Experiments were carried out to elucidate the optimum condition for each enzyme when acting on each substrate seperately. Results revealed that the highest relative activities for papain were achieved with E/S 0.06 , 0.29, 0.19 and pH 7.2, 7.0, 7.0 for soybean, sesame,and rice bran meal proteins, respectively. Optimum temperature for papain while hydrolysing the three substrates was 50 ºC. When using bromelain optimum E/S resulting in highest relative activities were 0.067, 0.058 and 0.21 for soybean, sesame,and rice bran meal protein, respectively. Optimum pH was 6.0 and optimum temperature was 45 ºC for bromelain when hydrolysing the protein of the three substrates. A numerical correlation of enzymatic behaviour for the different substrates was calculated.Proteínas de haba de soja, semilla de sésamo y harina de germen de arroz se hidrolizaron con dos enzimas, denominadas, papaina y bromelaina. Se han llevado a cabo experimentos para determinar las condiciones óptimas de cada enzima cuando actúan separadamente sobre cada sustrato. Los resultados mostraron que las mayores actividades relativas para la papaina se consiguieron con una E/S 0,06, 0,29, 0,19 y un pH 7.2, 7.0, 7.0 para las proteínas de haba de soja, sésamo y harina de germen de arroz, respectivamente. La temperatura óptima para la papaina durante la hidrólisis de los tres sustratos fue de 50 ºC. Cuando se usa bromelaina las relaciones E/S óptimas que proporcionaron mayor actividad relativa fueron 0.067, 0.058 y 0.21 para las proteínas de habas de soja, sésamo y harina de germen de arroz respectivamente. El pH óptimo fue 6.0 y la temperatura óptima 45 ºC para la bromelaina cuando se hidroliza la proteína de los tres sustratos. Con estos datos se hizo una correlación numérica del comportamiento enzimático para los diferentes sustratos

Development of a new medium containing date syrup for production of bleomycin by Streptomyces mobaraensis ATCC 15003 using response surface methodology

A combined statistical approach of orthogonal design and polynomial regression were applied to optimize the composition and concentration of a liquid fermentation medium for the production of bleomycin (BLM) by Streptomyces mobaraensis. Optimal conditions for maximal productivity were determined based on eight parameters at three different levels. The sources of carbon and nitrogen concentration and their interactions with other precursors were found to be statistically significant factors. When date syrup was used as an additional carbon source, higher BLM amount was obtained in comparison to glucose. It was found that the optimum nitrogen source was achieved with the use of soyabean meal. The combined orthogonal design and response surface methodology predicted optimal conditions for production of BLM to be 138 mg dl-1. A confirmatory experiment of the optimal medium composition produced 142 mg dl-1 in the fifth day fermentation at 30°C. The complex medium containing 40 gml-1 date syrup as additional carbon source enhanced the production of BLM by 73%. The combined statistical approach enabled rapid identification and integration of key medium parameters for optimizing secondary metabolite production and could be very useful in pharma-ceutical screening programs.Keywords: Bleomycin, Streptomyces mobaraensis, orthogonal design, medium optimization, date syrupAfrican Journal of Biotechnology Vol. 9(33), pp. 5450-5459, 16 August, 201