Reducing CO2 Emissions in the Upper Midwest: Technology, Resources, Economics, and Policy

We develop scenarios for reducing carbon dioxide emissions from the electricity sector in the upper Midwest (Wisconsin, Illinois, Minnesota, Iowa, North Dakota, South Dakota, Montana, Wyoming, and Manitoba) by 80% relative to 1990 levels. The report has three major components: 1) an inventory of CO2 emissions from all fossil fuel combustion in the region from 1960-2001, subdividing by economic sector and specific electricity generating station; 2) an evaluation of all electricity resources in the region and all technologies for utilizing them, taking into account the overall scale of the resource, technology costs, and other issues that influence the selection of a certain technology; and 3) the development of a simulation model to examine the impact of various factors (policies, prices, technologies, resources) on the regional electricity supply and its emissions from 2005-2055.Environmental Economics and Policy,

Evolution of male life histories and age-dependent sexual signals under female choice

Sexual selection theory models evolution of sexual signals and preferences using simple life histories. However, life-history models predict that males benefit from increasing sexual investment approaching old age, producing age-dependent sexual traits. Age-dependent traits require time and energy to grow, and will not fully mature before individuals enter mating competition. Early evolutionary stages pose several problems for these traits. Age-dependent traits suffer from strong viability selection and gain little benefit from mate choice when rare. Few males will grow large traits, and they will rarely encounter choosy females. The evolutionary origins of age-dependent traits therefore remain unclear. I used numerical simulations to analyze evolution of preferences, condition (viability) and traits in an age-structured population. Traits in the model depended on age and condition (“good genes”) in a population with no genetic drift. I asked (1) if age-dependent indicator traits and their preferences can originate depending on the strength of selection and the size of the trait; (2) which mode of development (age-dependent versus age-independent) eventually predominates when both modes occur in the population; and (3) if age-independent traits can invade a population with age-dependent traits. Age-dependent traits evolve under weaker selection and at smaller sizes than age-independent traits. This result held in isolation and when the types co-occur. Evolution of age-independent traits depends only on trait size, whereas evolution of age-dependent traits depends on both strength of selection and growth rate. Invasion of age-independence into populations with established traits followed a similar pattern with age-dependence predominating at small trait sizes. I suggest that reduced adult mortality facilitates sexual selection by favoring the evolution of age-dependent sexual signals under weak selection

Safety and Immunogenicity Following Administration of a Live, Attenuated Monovalent 2009 H1N1 Influenza Vaccine to Children and Adults in Two Randomized Controlled Trials

BACKGROUND: The safety, tolerability, and immunogenicity of a monovalent intranasal 2009 A/H1N1 live attenuated influenza vaccine (LAIV) were evaluated in children and adults. METHODS/PRINCIPAL FINDINGS: Two randomized, double-blind, placebo-controlled studies were completed in children (2-17 y) and adults (18-49 y). Subjects were assigned 4:1 to receive 2 doses of H1N1 LAIV or placebo 28 days apart. The primary safety endpoint was fever ≥38.3°C during days 1-8 after the first dose; the primary immunogenicity endpoint was the proportion of subjects experiencing a postdose seroresponse. Solicited symptoms and adverse events were recorded for 14 days after each dose and safety data were collected for 180 days post-final dose. In total, 326 children (H1N1 LAIV, n = 261; placebo, n = 65) and 300 adults (H1N1 LAIV, n = 240; placebo, n = 60) were enrolled. After dose 1, fever ≥38.3°C occurred in 4 (1.5%) pediatric vaccine recipients and 1 (1.5%) placebo recipient (rate difference, 0%; 95% CI: -6.4%, 3.1%). No adults experienced fever following dose 1. Seroresponse rates in children (H1N1 LAIV vs. placebo) were 11.1% vs. 6.3% after dose 1 (rate difference, 4.8%; 95% CI: -9.6%, 13.8%) and 32.0% vs. 14.5% after dose 2 (rate difference, 17.5%; 95% CI: 5.5%, 27.1%). Seroresponse rates in adults were 6.1% vs. 0% (rate difference, 6.1%; 95% CI: -5.6%, 12.6%) and 14.9% vs. 5.6% (rate difference, 9.3%; 95% CI: -0.8%, 16.3%) after dose 1 and dose 2, respectively. Solicited symptoms after dose 1 (H1N1 LAIV vs. placebo) occurred in 37.5% vs. 32.3% of children and 41.7% vs. 31.7% of adults. Solicited symptoms occurred less frequently after dose 2 in adults and children. No vaccine-related serious adverse events occurred. CONCLUSIONS/SIGNIFICANCE: In subjects aged 2 to 49 years, two doses of H1N1 LAIV have a safety and immunogenicity profile similar to other previously studied and efficacious formulations of seasonal trivalent LAIV. TRIAL REGISTRATION: ClinicalTrials.gov NCT00946101, NCT00945893

Electrically addressable vesicles: Tools for dielectrophoresis metrology

Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered electrical propserties to enable identifiable dielectrophoretic responses in microfabricated systems. These electrically addressable vesicles (EAVs) enable the creation of electrically distinct populations of test particles for DEP. EAVs offer control of both their inner aqueous core and outer membrane properties; by encapsulating solutions of different electrolyte strength inside the vesicle and by incorporating functionalized phospholipids containing poly(ethylene glycol) (PEG) brushes attached to their hydrophilic headgroup in the vesicle membrane, we demonstrate control of the vesicles’ electrical polarizabilities. This combined with the ability to encode information about the properties of the vesicle in its fluorescence signature forms the first steps toward the development of EAV populations as metrology tools for any DEP-based microsystem.National Institutes of Health (U.S.) (Grant RR199652)National Institutes of Health (U.S.) (Grant EB005753)Merck/CSBi (Fellowship)Solomon Buchsbaum AT&T Research Fun

Probiotic lactobacilli inhibit early stages of Candida albicans biofilm development by reducing their growth, cell adhesion, and filamentation

We evaluated the inhibitory effects of the probiotic Lactobacillus species on different phases of Candida albicans biofilm development. Quantification of biofilm growth and ultrastructural analyses were performed on C. albicans biofilms treated with Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. Planktonic lactobacilli induced a significant reduction (p\ua0\ua00.05), but significantly reduced the early stages of Candida biofilm formation (p\ua

Reducing CO2 Emissions in the Upper Midwest: Technology, Resources, Economics, and Policy

We develop scenarios for reducing carbon dioxide emissions from the electricity sector in the upper Midwest (Wisconsin, Illinois, Minnesota, Iowa, North Dakota, South Dakota, Montana, Wyoming, and Manitoba) by 80% relative to 1990 levels. The report has three major components: 1) an inventory of CO2 emissions from all fossil fuel combustion in the region from 1960-2001, subdividing by economic sector and specific electricity generating station; 2) an evaluation of all electricity resources in the region and all technologies for utilizing them, taking into account the overall scale of the resource, technology costs, and other issues that influence the selection of a certain technology; and 3) the development of a simulation model to examine the impact of various factors (policies, prices, technologies, resources) on the regional electricity supply and its emissions from 2005-2055

Reducing CO2 Emissions in the Upper Midwest: Technology, Resources, Economics, and Policy

We develop scenarios for reducing carbon dioxide emissions from the electricity sector in the upper Midwest (Wisconsin, Illinois, Minnesota, Iowa, North Dakota, South Dakota, Montana, Wyoming, and Manitoba) by 80% relative to 1990 levels. The report has three major components: 1) an inventory of CO2 emissions from all fossil fuel combustion in the region from 1960-2001, subdividing by economic sector and specific electricity generating station; 2) an evaluation of all electricity resources in the region and all technologies for utilizing them, taking into account the overall scale of the resource, technology costs, and other issues that influence the selection of a certain technology; and 3) the development of a simulation model to examine the impact of various factors (policies, prices, technologies, resources) on the regional electricity supply and its emissions from 2005-2055

1_IR_observed vs. expected

#### 1. Observed vs. expected IR ### Data sheet includes obsetved and simulated (expected) values from both populations (NY and CA), given in 4 ways. #### (1) Adults in sample = known breeders (according to CERVUS) at Scale = Broad local scale #### (2-4) Adults in sample = All local adults at three different scales: Scale = Broad local scale, Intermediate local scale, and neighborhood scale. ### Analyses in the manuscript are run (1) with known breeders (according to CERVUS) at Scale = Broad local scale, and (2-4) at each of the three scales, using all local adults. #### All analyses are subsetted by population (CA or NY) and use the following model: model1<-lm(IR~Treatment) summary(model1

Code_MolEcol_Townsend et al 2018

Description of data files and associated cod