We evaluated the inhibitory effects of the probiotic Lactobacillus species on different phases of Candida albicans biofilm development. Quantification of biofilm growth and ultrastructural analyses were performed on C. albicans biofilms treated with Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. Planktonic lactobacilli induced a significant reduction (p\ua0\ua00.05), but significantly reduced the early stages of Candida biofilm formation (p\ua

A Maldonado-Barragan

A Murzyn

A Roy

AL Servin

C Ceresa

C Parolin

C Simark-Mattsson

CF Orsi

CG Rizzello

D Li

E Kheradmand

E Kraft-Bodi

E Zakaria Gomaa

EH Ryu

FH Mendonça

GA Kohler

H Hu

H Li

H Wang

H. M. H. N. Bandara

HM Bandara

HT Taff

I Fidan

JC Sardi

JE Ericson

JE Nett

JH Meurman

JJ Coleman

JM Anderson

K Hatakka

KH Ishikawa

L Alcazar-Fuoli

L Rodrigues

L Sherry

Lakshman P. Samaranayake

LP Samaranayake

LR Rodrigues

M Atanassova

M Polke

M Sanguinetti

M Strus

Marcia Pinto Alves Mayer

MB Miller

MC Arendrup

MC Verdenelli

ME Falagas

P Hasslof

PG Pappas

S Kucharikova

SF Vilela

SM Kovachev

SY Chew

T Jones

VH Matsubara

Victor Haruo Matsubara

Y Jin

Yi Wang

We evaluated the inhibitory effects of the probiotic Lactobacillus species on different phases of Candida albicans biofilm development. Quantification of biofilm growth and ultrastructural analyses were performed on C. albicans biofilms treated with Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. Planktonic lactobacilli induced a significant reduction (p &lt; 0.05) in the number of biofilm cells (25.5-61.8 %) depending on the probiotic strain and the biofilm phase. L. rhamnosus supernatants had no significant effect on the mature biofilm (p &gt; 0.05), but significantly reduced the early stages of Candida biofilm formation (p &lt; 0.01). Microscopic analyses revealed that L. rhamnosus suspensions reduced Candida hyphal differentiation, leading to a predominance of budding growth. All lactobacilli negatively impacted C. albicans yeast-to-hyphae differentiation and biofilm formation. The inhibitory effects of the probiotic Lactobacillus on C. albicans entailed both cell-cell interactions and secretion of exometabolites that may impact on pathogenic attributes associated with C. albicans colonization on host surfaces and yeast filamentation. This study clarifies, for the first time, the mechanics of how Lactobacillus species may antagonize C. albicans host colonization. Our data elucidate the inhibitory mechanisms that define the probiotic candicidal activity of lactobacilli, thus supporting their utility as an adjunctive therapeutic mode against mucosal candidal infections.</p

Matsubara, Victor Haruo

Wang, Yi

Bandara, H M H N

Mayer, Marcia Pinto Alves

Samaranayake, Lakshman P

Explore Bristol Research

Matsubara, V. H., Wang, Y., Bandara, H. M. H. N., Mayer, M. P. A., &Samaranayake, L. P. (2016). Probiotic lactobacilli inhibit early stagesof Candida albicans biofilm development by reducing their growth, celladhesion, and filamentation. Applied Microbiology and Biotechnology,100(14), 6415-6426. https://doi.org/10.1007/s00253-016-7527-3Peer reviewed versionLink to published version (if available):10.1007/s00253-016-7527-3Link to publication record on the Bristol Research PortalPDF-documentUniversity of Bristol – Bristol Research PortalGeneral rightsThis document is made available in accordance with publisher policies. Please cite only thepublished version using the reference above. Full terms of use are available:http://www.bristol.ac.uk/red/research-policy/pure/user-guides/brp-terms/051015GROUPSx 106 (CFU/ml) L. rhamnosus LR32 (24h)*** ****** ******0246GROUPSx 106 (CFU/ml) L. rhamnosus LR32 (6h)* * * * **24	  h	   48	  h	  L.	  rhamnosus	  A	  02468GROUPSx 106 (CFU/ml) L. casei L324m (6h)*** *** ****** **051015GROUPSx 106 (CFU/ml) L. casei L324m (24h)******** ** ***24	  h	   48	  h	  L.	  casei	  B	  051015GROUPSx 106 (CFU/ml) L. acidophilus NCFM (24h)** **** *** ***02468GROUPSx 106 (CFU/ml) L. acidophilus NCFM (6h)****** ***** **24	  h	   48	  h	  L.	  acidophillus	  C	  0246810GROUPSx 106 (CFU/ml) L. rhamnosus LR32 (6h)** *05101520GROUPSx 106 (CFU/ml) L. rhamnosus LR32 (24h)*** ***24	  h	   48	  h	  D	  05101520GROUPSx 106 (CFU/ml) L. casei L324m (24h)* *0246810GROUPSx 106 (CFU/ml) L. casei L324m (6h)* *24	  h	   48	  h	  E	  05101520GROUPSx 106 (CFU/ml) L. acidophilus NCFM (24h)**0246810GROUPSx 106 (CFU/ml) L. acidophilus NCFM (6h)** * ***24	  h	   48	  h	  F	  C.	  albicans	  ATCC	  SC5314	   C. albicans 75  Control1.0 x 1075.0 x 1062.5 x 1061.25 x 1066.25 x 105

English

Probiotic lactobacilli inhibit early stages of <i>Candida albicans</i> biofilm development by reducing their growth, cell adhesion, and filamentation

                          Matsubara, V. H., Wang, Y., Bandara, H. M. H. N., Mayer, M. P. A., &Samaranayake, L. P. (2016). Probiotic lactobacilli inhibit early stages ofCandida albicans biofilm development by reducing their growth, celladhesion, and filamentation. Applied Microbiology and Biotechnology,100(14), 6415-6426. https://doi.org/10.1007/s00253-016-7527-3Peer reviewed versionLink to published version (if available):10.1007/s00253-016-7527-3Link to publication record in Explore Bristol ResearchPDF-documentUniversity of Bristol - Explore Bristol ResearchGeneral rightsThis document is made available in accordance with publisher policies. Please cite only the publishedversion using the reference above. Full terms of use are available:http://www.bristol.ac.uk/pure/about/ebr-terms  Biofilm viable cells reduction (%)  C. albicans ATCC SC5314  C. albicans 75  Initial Colonization  Maturation  Initial Colonization  Maturation Probiotic strain  Mean SD  Mean SD  Mean SD  Mean SD L. rhamnosus LR32  32.0 6.4  61.8* 8.3  31.8 12.2  39.8 6.2 L. casei L324m  59.0 6.9  54.7 4.6  27.7 5.0  36.6 3.3 L. acidophilus NCFM  56.3 4.2  34.2* 7.0  27.4 5.5  25.5 9.1 * Significant difference between initial colonization and maturation (p<0.05).  Table 1. Percentage of reduction of viable C. albicans cells on biofilms after treatment with probiotic bacteria suspension (1 x 107 probiotic cells/ml) during initial colonization (24 h) and maturation (48 h) phases.   

Crossref

Applied Microbiology and Biotechnology