Prediction of thermal radiative properties (300–1000 K) of La2NiO4+δ ceramics.

A multiscale numerical model is developed to predict the thermal radiative properties (TRP) of rough La2NiO4+δ coatings. The model integrates intrinsic and extrinsic contributions related to the chemical composition and the texture, respectively. High-temperature infrared reflectivity and thermogravimetric measurements on a La2NiO4+δ single crystal make it possible to understand the role of the excess oxygen in the intrinsic TRP. We show that dense ceramics with thicknesses higher than 4 μm are optically thick, and that one can adjust the surface roughness parameters to predict their TRP

Pichia pastoris versus Saccharomyces cerevisiae:a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor

BACKGROUND: Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is a glycoprotein that has been approved by the FDA for the treatment of neutropenia and leukemia in combination with chemotherapies. Recombinant hGM-CSF is produced industrially using the baker's yeast, Saccharomyces cerevisiae, by large-scale fermentation. The methylotrophic yeast, Pichia pastoris, has emerged as an alternative host cell system due to its shorter and less immunogenic glycosylation pattern together with higher cell density growth and higher secreted protein yield than S. cerevisiae. In this study, we compared the pipeline from gene to recombinant protein in these two yeasts. RESULTS: Codon optimization in silico for both yeast species showed no difference in frequent codon usage. However, rhGM-CSF expressed from S. cerevisiae BY4742 showed a significant discrepancy in molecular weight from those of P. pastoris X33. Analysis showed purified rhGM-CSF species with molecular weights ranging from 30 to more than 60 kDa. Fed-batch fermentation over 72 h showed that rhGM-CSF was more highly secreted from P. pastoris than S. cerevisiae (285 and 64 mg total secreted protein/L, respectively). Ion exchange chromatography gave higher purity and recovery than hydrophobic interaction chromatography. Purified rhGM-CSF from P. pastoris was 327 times more potent than rhGM-CSF from S. cerevisiae in terms of proliferative stimulating capacity on the hGM-CSF-dependent cell line, TF-1. CONCLUSION: Our data support a view that the methylotrophic yeast P. pastoris is an effective recombinant host for heterologous rhGM-CSF production

Planning for Developing Students’ Statistical Literacy: A Research-Informed Framework Development

Teachers’ planning as a research area still receives little attention. This study focuses on developing a research-informed framework to assess teachers’ statistics lesson plans. Using a design-based research approach, we developed a framework that includes (a) two criteria related to learning intentions (clear and comprehensive), (b) four criteria related to task features (statistical literacy, statistical investigation, real data, multiple representations), and (c) one related to constructivist lessons. Then, the team provided professional development on statistical literacy and ways to elicit and develop it in the classroom. After that, the teachers discussed the framework and used it to revise their lesson plans. Also, teachers provided feedback to revise the framework. We discuss how the framework can offer a tool for researchers to examine teacher planning competence and teachers to reflect on their practice

Croissance et caractérisations de couches minces d'oxydes à constante diélectrique élevée sur silicium (Etude de la sensibilité de condensateurs MOS aux rayonnements ionisants)

Ce travail est consacré à la croissance sur substrat de silicium de films minces d oxydes d yttrium (Y2O3) et l oxyde de cérium (CeO2). La technique de dépôt utilisée est la pulvérisation cathodique RF magnétron. Y2O3 est déposé à partir d une cible d yttrium métallique, la pulvérisation s effectuant à partir d un mélange gazeux Argon / Oxygène. Les couches minces d oxyde de cérium sont obtenues par pulvérisation d une cible de CeO2 par de l Argon. La température de croissance est comprise entre 200 C et 800 C. Le recuit éventuel des films d oxyde est effectué soit par un recuit thermique conventionnel, soit par une technique de recuit rapide. Dans un 1er temps, nous présentons, en fonction des paramètres de croissance, les propriétés structurales extraites de mesures de diffraction de rayons X et de spectroscopie Raman. Dans un 2e temps sont exposés les résultats des mesures électriques de type capacité-tension effectués sur les condensateurs de type MOS à base des couches d Y2O3 et de CeO2. Leur analyse permet de qualifier et quantifier les défauts électriquement actifs que sont les charges dans l oxyde et les états à l interface oxyde / silicium, dont la densité dépend des conditions de croissance et de recuit. Finalement, une étude de la sensibilité de ces dispositifs aux rayonnements gamma et aux neutrons est présentée. Une corrélation est faite entre la qualité cristalline des couches minces, la densité de charges tant dans l oxyde qu à l interface oxyde/silicium et la sensibilité des dispositifs. Ces résultats montrent en particulier que pour les condensateurs à base d Y2O3, des densités de défauts importantes confèrent une plus grande sensibilité aux rayons gammaThis work is devoted to the growth on oxide thin film silicon substrate of elements of the family of rare earths, the yttrium oxide (Y2O3) and cerium (CeO2) oxidizes it. The technique of deposit used is r.f. magnetron sputtering (13.56 MHz). The yttrium oxide is deposited starting from a metal yttrium target, pulverization being carried out starting from a gas mixture Argon/Oxygen. The thin layers of cerium oxide are obtained by pulverization of a target of CeO2 by Argon. The temperature of growth lies between 200 C and 800 C. The possible annealing of oxide films is carried out either by a conventional thermal annealing, or by a fast technique of annealing. In the beginning, we present, according to the growth s parameters, the structural properties extracted from measurements of x-rays diffraction and Raman spectroscopy. Then, we present the results of the electrical measurements (capacity-tension mode) taken on the MOS capacitors containing the Y2O3 or CeO2 layers. Their analysis makes it possible to qualify and to quantify the active defects electrical that are the charge in oxide and the states in the interface oxidizes/silicon, of which the density depends on the growth and annealing conditions. Finally, a sensitivity study of these devices with the gamma rays and with the neutrons is presented. A correlation is made between the crystalline quality of the thin film, the charge density as well in oxide as with the interface/silicon oxidizes, and the sensitivity of the devices. These results show in particular that for the capacitor containing Y2O3 layer, with the important densities of defects confer a very high sensitivity to the gamma rays.CAEN-BU Sciences et STAPS (141182103) / SudocSudocFranceF

The integral wavelet transform in weighted Sobolev spaces

The integral wavelet transform is defined in weighted Sobolev spaces, in which some properties of the transform as well as its asymptotical behaviour for small dilation parameter are studied

60Co gamma irradiation effects on electrical characteristics of Al/Y2O3/n-Si/Al capacitors

International audienc

Croissance de couches minces d'oxyde de Cérium pour la réalisation de dosimètres et/ou de détecteurs d’irradiation par pulvérisation cathodique RF magnétron

International audienc

Croissance de couches minces d'oxyde de cérium sur un substrat de silicium

International audienc

Genome-wide association study identifies susceptibility loci for dengue shock syndrome at MICB and PLCE1.

Hypovolemic shock (dengue shock syndrome (DSS)) is the most common life-threatening complication of dengue. We conducted a genome-wide association study of 2,008 pediatric cases treated for DSS and 2,018 controls from Vietnam. Replication of the most significantly associated markers was carried out in an independent Vietnamese sample of 1,737 cases and 2,934 controls. SNPs at two loci showed genome-wide significant association with DSS. We identified a susceptibility locus at MICB (major histocompatibility complex (MHC) class I polypeptide-related sequence B), which was within the broad MHC region on chromosome 6 but outside the class I and class II HLA loci (rs3132468, P(meta) = 4.41 × 10(-11), per-allele odds ratio (OR) = 1.34 (95% confidence interval: 1.23-1.46)). We identified associated variants within PLCE1 (phospholipase C, epsilon 1) on chromosome 10 (rs3765524, P(meta) = 3.08 × 10(-10), per-allele OR = 0.80 (95% confidence interval: 0.75-0.86)). We identify two loci associated with susceptibility to DSS in people with dengue, suggesting possible mechanisms for this severe complication of dengue