1,964 research outputs found
Vertical structure, turbulent mixing and fluxes during Lagrangian observations of an upwelling filament system off Northwest Iberia
In August 1998, a recurrent filament located near 42°N off Galicia was sampled as part of the OMEX-II project. Lagrangian and other observations were made on the shelf where the filament arose and offshore in the filament itself under upwelling favourable but fluctuating winds. The shelf drift experiment monitored a change from southward to weak northward net flow as the winds decreased to zero. Shipborne {ADCP} measurements showed that the shelf was supplying decreasing volumes of water to the filament as the wind speeds decreased. At the shelf edge the internal tide was larger than can be explained by local forcing and there were many unusually large high frequency internal waves with a quasi-sinusoidal form. Turbulence observations revealed enhanced dissipation rates and vertical eddy diffusion coefficients within the shelf thermocline (of order 1 cm2 s�1), which appeared to be caused by the breaking of internal wave. A second Lagrangian experiment was executed in the filament some 120 km offshore, which again coincided with a period of wind relaxation. Cross-sections revealed a double cold core and that the offshore flow was limited to a thin surface layer. Substantial onshore flow occurred below 50 m in the centre of the filament, while the strongest and deepest offshore flow coincided with its northern boundary. Turbulent kinetic energy dissipation rate measurements showed very weak mixing below 15 m in the filament core, but enhanced mixing at its boundaries. Four mixed layer drifters released in the filament initially indicated convergence at its southern boundary, marked by strong temperature and salinity contrasts. After the wind became more favourable for upwelling, the drifters accelerated. One drifter traced the full extent of the filament, while the other three escaped from it and began to circulate cyclonically over 28 days in a 100 km diameter loop back towards their release point. Although strong mesoscale activity linked the shelf and ocean regimes, offshore transport in the filament was weak at the time of the experiment and vertical and horizontal re-circulations on a variety of time scales were important. There was sufficient vertical mixing in the thermocline to cause it to thicken and draw some heat into the lower layers during the summer months on the shelf. The amount of heat involved was too little to have a significant impact on the development of a filament over a typical lifetime of a week
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Induction of Synovial Apoptosis by Gene Transfer and Peptide Mediated Protein Transduction
Stellar Disk Truncations: Where do we stand ?
In the light of several recent developments we revisit the phenomenon of
galactic stellar disk truncations. Even 25 years since the first paper on outer
breaks in the radial light profiles of spiral galaxies, their origin is still
unclear. The two most promising explanations are that these 'outer edges'
either trace the maximum angular momentum during the galaxy formation epoch, or
are associated with global star formation thresholds. Depending on their true
physical nature, these outer edges may represent an improved size
characteristic (e.g., as compared to D_25) and might contain fossil evidence
imprinted by the galaxy formation and evolutionary history. We will address
several observational aspects of disk truncations: their existence, not only in
normal HSB galaxies, but also in LSB and even dwarf galaxies; their detailed
shape, not sharp cut-offs as thought before, but in fact demarcating the start
of a region with a steeper exponential distribution of starlight; their
possible association with bars; as well as problems related to the
line-of-sight integration for edge-on galaxies (the main targets for truncation
searches so far). Taken together, these observations currently favour the
star-formation threshold model, but more work is necessary to implement the
truncations as adequate parameters characterising galactic disks.Comment: LaTeX, 10 pages, 6 figures, presented at the "Penetrating Bars
through Masks of Cosmic Dust" conference in South Africa, proceedings
published by Kluwer, and edited by Block, D.L., Freeman, K.C., Puerari, I., &
Groess, R; v3 to match published versio
Cost-effectiveness of sentinel lymph node biopsy vs inguinofemoral lymphadenectomy in women with vulval cancer
background: This study examines the cost-effectiveness of sentinel lymph node biopsy, a potentially less morbid procedure, compared with inguinofemoral lymphadenectomy (IFL) among women with stage I and stage II vulval squamous cell carcinoma. methods: A model-based economic evaluation was undertaken based on clinical evidence from a systematic review of published sources. A decision tree model was developed with the structure being informed by clinical input, taking the perspective of the health-care provider. results: For overall survival for 2 years, IFL was found to be the most cost-effective option and dominated all other strategies, being the least costly and most effective. For morbidity-free related outcomes for 2 years, sentinel lymph node (SLN) biopsy with 99mTc and blue dye and haematoxylin & eosin (H&E) histopathology, with ultrastaging and immunohistochemistry reserved for those that test negative following H&E is likely to be the most effective approach. conclusion: SLN biopsy using 99mTc and blue dye with ultrastaging may be considered the most cost-effective strategy based on the outcome of survival free of morbidity for 2 years. The findings here also indicate that using blue dye and H&E for the identification of the SLN and the identification of metastasis, respectively, are not sensitive enough to be used on their own
Unexpected host dependency of Antarctic Nanohaloarchaeota
In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarchaeon Halorubrum lacusprofundi for growth. By performing growth using enrichments and fluorescence-activated cell sorting, we demonstrated successful cultivation of Candidatus Nanohaloarchaeum antarcticus, purification of Ca. Nha. antarcticus away from other species, and growth and verification of Ca. Nha. antarcticus with Hrr. lacusprofundi; these findings are analogous to those required for fulfilling Koch’s postulates. We use fluorescent in situ hybridization and transmission electron microscopy to assess cell structures and interactions; metagenomics to characterize enrichment taxa, generate metagenome assembled genomes, and interrogate Antarctic communities; and proteomics to assess metabolic pathways and speculate about the roles of certain proteins. Metagenome analysis indicates the presence of a single species, which is endemic to Antarctic hypersaline systems that support the growth of haloarchaea. The presence of unusually large proteins predicted to function in attachment and invasion of hosts plus the absence of key biosynthetic pathways (e.g., lipids) in metagenome assembled genomes of globally distributed Nanohaloarchaeota indicate that all members of the lineage have evolved as symbionts. Our work expands the range of archaeal symbiotic lifestyles and provides a genetically tractable model system for advancing understanding of the factors controlling microbial symbiotic relationships
Teaching Robust Argumentation Informed by the Nature of Science to Support Social Justice. Experiences from Two Projects in Lower Secondary Schools in Norway
Under embargo until: 2022-09-09This chapter suggests a set of design principles for science curricula that will enable students to produce evidence-based arguments expressing views related to their own interests. It is based on the assumption that the ability to construct evidence-based arguments strengthens students’ ability to promote their own views in the interest of social justice. This is of special importance for students not enculturated into such argumentation through their upbringing. To promote one’s own views in a debate means to critique others’ arguments, and especially to ensure one’s own arguments are resistent to criticism. Insight into the nature of science includes insights in how to construct sound arguments based on facts and research results. The discussion of design principles is based on an analysis of two science projects in two lower secondary schools in Norway (Grade 8). In the first project, students produced scientific claims based on evidence from their own practical experiments. In the second project, the students developed and applied a method for estimating energy use and carbon dioxide (CO2) emissions. The students used their findings to construct arguments related to local transport plans. The analysis focuses on challenges and successes in scaffolding students at different competence levels to successfully produce evidence-based arguments.acceptedVersio
Speciation Along Environmental Gradients
Traditional discussions of speciation are based on geographical patterns of species ranges. In allopatric speciation, long-term geographical isolation generates reproductively isolated and spatially segregated descendant species. In the absence of geographical barriers, diversification is hindered by gene flow. Yet a growing body of phylogenetic and experimental data suggests that closely related species often occur in sympatry or have adjacent ranges in regions over which environmental changes are gradual and do not prevent gene flow. Theory has identified a variety of evolutionary processes that can result in speciation under sympatric conditions, with some recent advances concentrating on the phenomenon of evolutionary branching. Here we establish a link between geographical patterns and ecological processes of speciation by studying evolutionary branching in spatially structured populations. We show that along an environmental gradient, evolutionary branching can occur much more easily than in non-spatial models. This facilitation is most pronounced for gradients of intermediate slope. Moreover, spatial evolutionary branching readily generates patterns of spatial segregation and abutment between the emerging species. Our results highlight the importance of local processes of adaptive divergence for geographical patterns of speciation, and caution against pitfalls of inferring past speciation processes from present biogeographical patterns
A ship rain gauge for use in high wind speeds.
A ship rain gauge has been developed that can be used under high wind speeds such as those experienced by ships at sea. The instrument has an improved aerodynamic design and an additional lateral collecting surface, which is effective especially with high wind speeds. The ship rain gauge has been calibrated at sea against a specially designed optical disdrometer. An accuracy of 2%–3% has been obtained for 6-hourly sums. The ship rain gauge has also successfully been tested at a test site of the German Weather Service and presently is used on research vessels and voluntary observing ship
The comparative osmoregulatory ability of two water beetle genera whose species span the fresh-hypersaline gradient in inland waters (Coleoptera: Dytiscidae, Hydrophilidae).
A better knowledge of the physiological basis of salinity tolerance is essential to understanding the ecology and evolutionary history of organisms that have colonized inland saline waters. Coleoptera are amongst the most diverse macroinvertebrates in inland waters, including saline habitats; however, the osmoregulatory strategies they employ to deal with osmotic stress remain unexplored. Survival and haemolymph osmotic concentration at different salinities were examined in adults of eight aquatic beetle species which inhabit different parts of the fresh-hypersaline gradient. Studied species belong to two unrelated genera which have invaded saline waters independently from freshwater ancestors; Nebrioporus (Dytiscidae) and Enochrus (Hydrophilidae). Their osmoregulatory strategy (osmoconformity or osmoregulation) was identified and osmotic capacity (the osmotic gradient between the animal's haemolymph and the external medium) was compared between species pairs co-habiting similar salinities in nature. We show that osmoregulatory capacity, rather than osmoconformity, has evolved independently in these different lineages. All species hyperegulated their haemolymph osmotic concentration in diluted waters; those living in fresh or low-salinity waters were unable to hyporegulate and survive in hyperosmotic media (> 340 mosmol kg(-1)). In contrast, the species which inhabit the hypo-hypersaline habitats were effective hyporegulators, maintaining their haemolymph osmolality within narrow limits (ca. 300 mosmol kg(-1)) across a wide range of external concentrations. The hypersaline species N. ceresyi and E. jesusarribasi tolerated conductivities up to 140 and 180 mS cm(-1), respectively, and maintained osmotic gradients over 3500 mosmol kg(-1), comparable to those of the most effective insect osmoregulators known to date. Syntopic species of both genera showed similar osmotic capacities and in general, osmotic responses correlated well with upper salinity levels occupied by individual species in nature. Therefore, osmoregulatory capacity may mediate habitat segregation amongst congeners across the salinity gradient
Multiple ITS Copies Reveal Extensive Hybridization within Rheum (Polygonaceae), a Genus That Has Undergone Rapid Radiation
During adaptive radiation events, characters can arise multiple times due to parallel evolution, but transfer of traits through hybridization provides an alternative explanation for the same character appearing in apparently non-sister lineages. The signature of hybridization can be detected in incongruence between phylogenies derived from different markers, or from the presence of two divergent versions of a nuclear marker such as ITS within one individual.In this study, we cloned and sequenced ITS regions for 30 species of the genus Rheum, and compared them with a cpDNA phylogeny. Seven species contained two divergent copies of ITS that resolved in different clades from one another in each case, indicating hybridization events too recent for concerted evolution to have homogenised the ITS sequences. Hybridization was also indicated in at least two further species via incongruence in their position between ITS and cpDNA phylogenies. None of the ITS sequences present in these nine species matched those detected in any other species, which provides tentative evidence against recent introgression as an explanation. Rheum globulosum, previously indicated by cpDNA to represent an independent origin of decumbent habit, is indicated by ITS to be part of clade of decumbent species, which acquired cpDNA of another clade via hybridization. However decumbent and glasshouse morphology are confirmed to have arisen three and two times, respectively.These findings suggested that hybridization among QTP species of Rheum has been extensive, and that a role of hybridization in diversification of Rheum requires investigation
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