Skin pigmentation in gilthead seabream (Sparus aurata l.) fed conventional and novel protein sources in diets deprived of fish meal

The pattern of yellowish pigmentation of the skin was assessed in gilthead seabream (Sparus aurata) fed for 12 weeks iso-proteic (45%) and iso-lipidic (20%) diets deprived of fish meal and containing either a blend of vegetable protein-rich ingredients or where graded levels of the vegetable protein blend were replaced by insect (Hermetia illucens\u201410%, 20% or 40%) pupae meal, poultry by-product meal (20%, 30% or 40%), red swamp crayfish meal (10%) and marine microalgae (Tisochrysis lutea and Tetraselmis suecica\u201410%) dried biomass. Digital images of fish fed diets differing in protein sources were analyzed by means of an automatic and non-invasive image analysis tool, in order to determine the number of yellow pixels and their dispersion on the frontal and lateral sides of the fish. The relationship between the total carotenoid concentration in the diet and the number of yellow pixels was investigated. Test diets differently affected gilthead seabream skin pigmentation both in the forefront and the operculum, due to their carotenoid content. The highest yellow pixels\u2019 number was observed with the diet containing microalgae. Fish fed poultry by-product meal were characterized by the lowest yellow pixels\u2019 number, diets containing insect meal had an intermediate coloring capacity. The vegetable control, the microalgae mix diet and the crayfish diet had significantly higher values of yellow pixels at both inspected skin sites

Linear Categorical Marginal Modeling of solicited symptoms in vaccine clinical trials

Analysis of the occurrence of adverse events, and in particular of solicited symptoms, following vaccination is often needed for the safety and benefit-risk evaluation of any candidate vaccine, and typically involves taking repeated measurements. In this article, it is shown that Linear Categorical Marginal Models (LCMMs) are well suited to take into account the dependencies in the data arising from the repeated measurements and provide detailed and useful information for comparing safety profiles of different products while remaining relatively easy to interpret. LCMMs are presented and applied to a Phase III clinical trial of a candidate meningococcal pediatric vaccine

Evaluation of Toxicant-Associated Fatty Liver Disease and Liver Neoplastic Progress in Sprague-Dawley Rats Treated with Low Doses of Aflatoxin B1 Alone or in Combination with Extremely Low Frequency Electromagnetic Fields

The term toxicant-associated fatty liver disease (TAFLD) has been proposed to describe fatty liver diseases connected to toxicants other than alcohol. Aflatoxins are mycotoxins commonly found as contaminants in foods and feeds, which are known liver toxicants and potential candidates as potential causes of TAFLD. Aflatoxin B1 (AFB1) was administered at low doses to Sprague-Daw-ley (SD) rats, alone or in combination with S-50 Hz an extremely low frequency electromagnetic field (ELFEMF), to study the evolution of TAFLD, preneoplastic and neoplastic lesions of the liver and the potential enhancing effect of lifespan exposure to ELFEMF. Steatosis, inflammation and foci of different types were significantly increased in both aflatoxin-treated males and females, which is consistent with a pattern of TAFLD. A significant increase in adenomas, cystic dilation of biliary ducts, hepatocellular hyperplasia and hypertrophy and oval cell hyperplasia were also observed in treated females only. The administration of low doses of AFB1 caused TAFLD in SD rats, inducing liver lesions encompassing fatty infiltration, foci of different types and adenomas. Furthermore, the pattern of change observed in preneoplastic liver lesions often included liver steatosis and steato-hepatitis (TASH). ELFEMF did not result in any enhancing or toxic effect in the liver of SD rats

Plasmonic nanorods for enhanced absorption in mid-wavelength infrared detectors

The absorption properties of HgCdTe-based infrared detectors can be greatly increased in the mid-infrared band, by incorporating nanostructured plasmonic arrays on the illuminated detector face. The array periodicity, combined with the excitation of surface plasmon-polariton stationary modes, enhances the absorption efficiency by a substantial amount, allowing to reduce in turn the HgCdTe absorption thickness

Challenges in multiphysics modeling of dual-band HgCdTe infrared detectors

We present three-dimensional simulations of HgCdTe-based focal plane arrays (FPAs) with two-color and dual-band sequential infrared pixels having realistic truncated-pyramid shape taking into account the presence of compositionally-graded transition layers. Simulations emphasize the importance of a full-wave approach to the electromagnetic problem, and the evaluations of the optical and diffusive contribution to inter-pixel crosstalk indicate the effectiveness of deep trenches to prevent diffusive crosstalk in both wavebands

Welfare and quality of farmed trout fed high plant protein diets. 2 innovative killing method effect on stress and quality indicators

AbstractThe fish stunning/slaughtering procedure has an important role both for the ethical aspect related to animal welfare and for the potential quality of the final products. Stressful harvest procedure and killing methods can negatively influence the post mortem biochemical processes with a consequent faster fish freshness loss. In particular, killing procedures causing a long agony are not humane and can shorten fish shelf life; others, more humane, can have risks for the consumers health (anaesthetics or chemical substances) or are not feasible in small/medium size fish industry (spiking and knocking). The aim of this study was to compare the traditional killing method (asphyxia - A) used for rainbow trout (Onchorynchus mykiss) with an innovative stunning/slaughtering method (two-stage electric stun: 2s at a 500Hz electric field of 2.5V/cm and then 58s at a 50Hz electric field of 0.75V/cm - E), through the study of their effects on stress and quality indicators in fish fed with traditional fish meal..

Fracture and damage localization in volcanic edifice rocks from El Hierro, Stromboli and Tenerife

© 2018 The Author(s). We present elastic wave velocity and strength data from a suite of three volcanic rocks taken from the volcanic edifices of El Hierro and Tenerife (Canary Islands, Spain), and Stromboli (Aeolian Islands, Italy). These rocks span a range of porosity and are taken from volcanoes that suffer from edifice instability. We measure elastic wave velocities at known incident angles to the generated through-going fault as a function of imposed strain, and examine the effect of the damage zone on P-wave velocity. Such data are important as field measurements of elastic wave tomography are key tools for understanding volcanic regions, yet hidden fractures are likely to have a significant effect on elastic wave velocity. We then use elastic wave velocity evolution to calculate concomitant crack density evolution which ranges from 0 to 0.17: highest values were correlated to the damage zone in rocks with the highest initial porosity