Slx5/Slx8-dependent ubiquitin hotspots on chromatin contribute to stress tolerance

Chromatin is a highly regulated environment, and protein association with chromatin is often controlled by post-translational modifications and the corresponding enzymatic machinery. Specifically, SUMO-targeted ubiquitin ligases (STUbLs) have emerged as key players in nuclear quality control, genome maintenance, and transcription. However, how STUbLs select specific substrates among myriads of SUMOylated proteins on chromatin remains unclear. Here, we reveal a remarkable co-localization of the budding yeast STUbL Slx5/Slx8 and ubiquitin at seven genomic loci that we term "ubiquitin hotspots". Ubiquitylation at these sites depends on Slx5/Slx8 and protein turnover on the Cdc48 segregase. We identify the transcription factor-like Ymr111c/Euc1 to associate with these sites and to be a critical determinant of ubiquitylation. Euc1 specifically targets Slx5/Slx8 to ubiquitin hotspots via bipartite binding of Slx5 that involves the Slx5 SUMO-interacting motifs and an additional, novel substrate recognition domain. Interestingly, the Euc1-ubiquitin hotspot pathway acts redundantly with chromatin modifiers of the H2A.Z and Rpd3L pathways in specific stress responses. Thus, our data suggest that STUbL-dependent ubiquitin hotspots shape chromatin during stress adaptation

Satellite passive microwave sea-ice concentration data set inter-comparison for Arctic summer conditions

We report on results of a systematic inter-comparison of 10 global sea-ice concentration (SIC) data products at 12.5 to 50.0 km grid resolution from satellite passive microwave (PMW) observations for the Arctic during summer. The products are compared against SIC and net ice surface fraction (ISF) - SIC minus the per-grid-cell melt pond fraction (MPF) on sea ice - as derived from MODerate resolution Imaging Spectroradiometer (MODIS) satellite observations and observed from ice-going vessels. Like in Kern et al. (2019), we group the 10 products based on the concept of the SIC retrieval used. Group I consists of products of the European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT) Ocean and Sea Ice Satellite Application Facility (OSI SAF) and European Space Agency (ESA) Climate Change Initiative (CCI) algorithms. Group II consists of products derived with the Comiso bootstrap algorithm and the National Oceanographic and Atmospheric Administration (NOAA) National Snow and Ice Data Center (NSIDC) SIC climate data record (CDR). Group III consists of Arctic Radiation and Turbulence Interaction Study (ARTIST) Sea Ice (ASI) and National Aeronautics and Space Administration (NASA) Team (NT) algorithm products, and group IV consists of products of the enhanced NASA Team algorithm (NT2). We find widespread positive and negative differences between PMW and MODIS SIC with magnitudes frequently reaching up to 20 %-25 % for groups I and III and up to 30 %-35 % for groups II and IV. On a pan-Arctic scale these differences may cancel out: Arctic average SIC from group I products agrees with MODIS within 2 %-5 % accuracy during the entire melt period from May through September. Group II and IV products overestimate MODIS Arctic average SIC by 5 %-10 %. Out of group III, ASI is similar to group I products while NT SIC underestimates MODIS Arctic average SIC by 5 %-10 %. These differences, when translated into the impact computing Arctic sea-ice area (SIA), match well with the differences in SIA between the four groups reported for the summer months by Kern et al. (2019). MODIS ISF is systematically overestimated by all products; NT provides the smallest overestimations (up to 25 %) and group II and IV products the largest overestimations (up to 45 %). The spatial distribution of the observed overestimation of MODIS ISF agrees reasonably well with the spatial distribution of the MODIS MPF and we find a robust linear relationship between PMW SIC and MODIS ISF for group I and III products during peak melt, i.e. July and August. We discuss different cases taking into account the expected influence of ice surface properties other than melt ponds, i.e. wet snow and coarse-grained snow/refrozen surface, on brightness temperatures and their ratios used as input to the SIC retrieval algorithms. Based on this discussion we identify the mismatch between the actually observed surface properties and those represented by the ice tie points as the most likely reason for (i) the observed differences between PMW SIC and MODIS ISF and for (ii) the often surprisingly small difference between PMW and MODIS SIC in areas of high melt pond fraction. We conclude that all 10 SIC products are highly inaccurate during summer melt. We hypothesize that the unknown number of melt pond signatures likely included in the ice tie points plays an important role - particularly for groups I and II - and recommend conducting further research in this field

Hierarchical Triggering of Star Formation by Superbubbles in W3/W4

It is generally believed that expanding superbubbles and mechanical feedback from massive stars trigger star formation, because there are numerous examples of superbubbles showing secondary star formation at their edges. However, while these systems show an age sequence, they do not provide strong evidence of a causal relationship. The W3/W4 Galactic star-forming complex suggests a three-generation hierarchy: the supergiant shell structures correspond to the oldest generation; these triggered the formation of IC 1795 in W3, the progenitor of a molecular superbubble; which in turn triggered the current star-forming episodes in the embedded regions W3-North, W3-Main, and W3-OH. We present UBV photometry and spectroscopic classifications for IC 1795, which show an age of 3 - 5 Myr. This age is intermediate between the reported 6 - 20 Myr age of the supergiant shell system, and the extremely young ages (10^4 - 10^5 yr) for the embedded knots of ultracompact HII regions, W3-North, W3-Main, and W3-OH. Thus, an age sequence is indeed confirmed for the entire W3/W4 hierarchical system. This therefore provides some of the first convincing evidence that superbubble action and mechanical feedback are indeed a triggering mechanism for star formation.Comment: 10 pages, 6 figures; accepted to the Astronomical Journal. Figure 2 included in this submission as JPE

Multipole Ordering and Fluctuations in f-Electron Systems

We investigate effects of multipole moments in f-electron systems both from phenomenological and microscopic viewpoints. First, we discuss significant effects of octupole moment on the magnetic susceptibility in a paramagnetic phase. It is found that even within mean-field approximation, the magnetic susceptibility deviates from the Curie-Weiss law due to interactions between dipole and octupole moments. Next, we proceed to a microscopic theory for multipole ordering on the basis of a j-j coupling scheme. After brief explanation of a method to derive multipole interactions from the $f$-electron model, we discuss several multipole ordered phases depending on lattice structure. Finally, we show our new development of the microscopic approach to the evaluation of multipole response functions. We apply fluctuation exchange approximation to the f-electron model, and evaluate multipole response functions.Comment: 7 pages, 4 figures, Proceedings of ASR-WYP-200

The Disjunctive Conception of Perceiving

John McDowell's conception of perceptual knowledge commits him to the claim that if I perceive that P then I am in a position to know that I perceive that P. In the first part of this essay, I present some reasons to be suspicious of this claim - reasons which derive from a general argument against 'luminosity' - and suggest that McDowell can reject this claim, while holding on to almost all of the rest of his conception of perceptual knowledge, by supplementing his existing disjunctive conception of experience with a new disjunctive conception of perceiving. In the second part of the essay, I present some reasons for thinking that one's justification, in cases of perceptual knowledge, consists not in the fact that one perceives that P but in the fact that one perceives such-and-such. I end by suggesting that the disjunctive conception of perceiving should be understood as a disjunctive conception of perceiving such-and-such

Cerebrospinal fluid biomarkers of axonal and synaptic degeneration in a population-based sample

Background: Neurofilament light (NfL) and neurogranin (Ng) are promising candidate AD biomarkers, reflecting axonal and synaptic damage, respectively. Since there is a need to understand the synaptic and axonal damage in preclinical Alzheimer’s disease (AD), we aimed to determine the cerebrospinal fluid (CSF) levels of NfL and Ng in cognitively unimpaired elderly from the Gothenburg H70 Birth Cohort Studies classified according to the amyloid/tau/neurodegeneration (A/T/N) system. Methods: The sample consisted of 258 cognitively unimpaired older adults (age 70, 129 women and 129 men) from the Gothenburg Birth Cohort Studies. We compared CSF NfL and Ng concentrations in A/T/N groups using Student’s T-test and ANCOVA. Results: CSF NfL concentration was higher in the A−T−N+ group (p=0.001) and the A−T+N+ group (p=0.006) compared with A−T−N−. CSF Ng concentration was higher in the A−T−N+, A−T+N+, A+T−N+, and A+T+N+ groups (p<0.0001) compared with A−T−N−. We found no difference in NfL or Ng concentration in A+ compared with A− (disregarding T− and N− status), whereas those with N+ had higher concentrations of NfL and Ng compared with N− (p<0.0001) (disregarding A− and T− status). Conclusions: CSF NfL and Ng concentrations are increased in cognitively normal older adults with biomarker evidence of tau pathology and neurodegeneration

ExTrA: Exoplanets in Transit and their Atmospheres

The ExTrA facility, located at La Silla observatory, will consist of a near-infrared multi-object spectrograph fed by three 60-cm telescopes. ExTrA will add the spectroscopic resolution to the traditional differential photometry method. This shall enable the fine correction of color-dependent systematics that would otherwise hinder ground-based observations. With both this novel method and an infrared-enabled efficiency, ExTrA aims to find transiting telluric planets orbiting in the habitable zone of bright nearby M dwarfs. It shall have the versatility to do so by running its own independent survey and also by concurrently following-up on the space candidates unveiled by K2 and TESS. The exoplanets detected by ExTrA will be amenable to atmospheric characterisation with VLTs, JWST, and ELTs and could give our first peek into an exo-life laboratory.Comment: 15 pages, 11 figures, SPIE 201

In-plane magnetic anisotropy of Fe atoms on Bi2_2Se3_3(111)

The robustness of the gapless topological surface state hosted by a 3D topological insulator against perturbations of magnetic origin has been the focus of recent investigations. We present a comprehensive study of the magnetic properties of Fe impurities on a prototypical 3D topological insulator Bi$_2$Se$_3$ using local low temperature scanning tunneling microscopy and integral x-ray magnetic circular dichroism techniques. Single Fe adatoms on the Bi$_2$Se$_3$ surface, in the coverage range $\approx 1$ are heavily relaxed into the surface and exhibit a magnetic easy axis within the surface-plane, contrary to what was assumed in recent investigations on the opening of a gap. Using \textit{ab initio} approaches, we demonstrate that an in-plane easy axis arises from the combination of the crystal field and dynamic hybridization effects.Comment: 5 pages, 3 figures, typos correcte