2,445 research outputs found

    A Probabilistic Model for Creep-Fatigue Failure

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    Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetases

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    The termination of protein synthesis is encoded by in-frame nonsense (stop) codons. Most organisms use three nonsense codons: UGA, UAG, and UAA. In contrast to sense codons, which are decoded by specific tRNAs, nonsense codons are decoded by proteins called release factors (RFs). Here we report the cloning of a mammalian RF cDNA by the use of monoclonal antibodies specific for rabbit RF. Functional studies showed that, when expressed in Escherichia coli, the protein encoded by this cDNA has in vitro biochemical characteristics similar to those of previously characterized mammalian RFs. DNA sequencing of this eukaryotic RF cDNA revealed a remarkable sequence similarity to bacterial and mitochondrial tryptophanyl-tRNA synthetases, with the greatest similarity confined to the synthetase active site, and no obvious similarity to bacterial RFs

    Guest Editorial

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    This is an editorial which introduces original papers produced on the theme of the supervision of social work practiceThis is an Accepted Manuscript of an article published by Taylor & Francis in Practice: Social Work in Action on September 2015, available online: http://dx.doi.org/10.1080/09503153.2015.1048053This guest editorial introduces the special edition on the supervision of social work practic

    The Post-Common Envelope and Pre-Cataclysmic Binary PG 1224+309

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    We have made extensive spectroscopic and photometric observations of PG 1224+309, a close binary containing a DA white dwarf primary and an M4+ secondary. The H alpha line is in emission due to irradiation of the M-star by the hot white dwarf and is seen to vary around the orbit. From the radial velocities of the H alpha line we derive a period of P = 0.258689 +/- 0.000004 days and a semi-amplitude of K_Halpha = 160 +/- 8 km/s. We estimate a correction Delta_K = 21 +/- 2 km/s, where K_M = K_Halpha + Delta_K. Radial velocity variations of the white dwarf reveal a semi-amplitude of K_WD = 112 +/- 14 km/s. The blue spectrum of the white dwarf is well fit by a synthetic spectrum having T_eff = 29,300 K and log(g) = 7.38. The white dwarf contributes 97% of the light at 4500 Angstroms and virtually all of the light blueward of 3800 Angstroms. No eclipses are observed. The mass inferred for the white dwarf depends on the assumed mass of the thin residual hydrogen envelope: 0.40 < M_WD < 0.45 solar masses for hydrogen envelope masses of 0 < M_H < 4.0E-4 solar masses. We argue that the mass of the white dwarf is closer to 0.45 solar masses, hence it appears that the white dwarf has a relatively large residual hydrogen envelope. The mass of the M-star is then M_M = 0.28 +/- 0.05 solar masses, and the inclination is i = 77 +/- 7 degrees. We discuss briefly how PG 1224+309 may be used to constrain theories of close binary star evolution, and the past and future histories of PG 1224+309 itself. The star is both a ``post-common envelope'' star and a ``pre-cataclysmic binary'' star. Mass transfer by Roche-lobe overflow should commence in about 10 Gyr.Comment: 17 pages, 8 figures, AAS LaTeX, to appear in AJ, March 199

    The Wicked Machinery of Government: Malta and the Problems of Continuity under the New Model Administration

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    This is a study focused on the early years of British rule in Malta (1800-1813). It explores the application to the island of the “new model” of colonial government, one based on direct rule from London mediated by the continuation of existing laws and institutions. Systemic deficiencies are identified. These tended to undermine the effectiveness of direct British rule. This study also reveals, in the context of legal and constitutional continuity, unresolved tensions between modernity and tradition. The political stability of the island was damaged and the possibility of continued British possession was threatened

    Cirrus cloud identification from airborne far-infrared and mid-infrared spectra

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    Airborne interferometric data, obtained from the Cirrus Coupled Cloud-Radiation Experiment (CIRCCREX) and from the PiknMix-F field campaign, are used to test the ability of a machine learning cloud identification and classification algorithm (CIC). Data comprise a set of spectral radiances measured by the Tropospheric Airborne Fourier Transform Spectrometer (TAFTS) and the Airborne Research Interferometer Evaluation System (ARIES). Co-located measurements of the two sensors allow observations of the upwelling radiance for clear and cloudy conditions across the far-and mid-infrared part of the spectrum. Theoretical sensitivity studies show that the performance of the CIC algorithm improves with cloud altitude. These tests also suggest that, for conditions encompassing those sampled by the flight campaigns, the additional information contained within the far-infrared improves the algorithm's performance compared to using mid-infrared data only. When the CIC is applied to the airborne radiance measurements, the classification performance of the algorithm is very high. However, in this case, the limited temporal and spatial variability in the measured spectra results in a less obvious advantage being apparent when using both mid-and far-infrared radiances compared to using mid-infrared information only. These results suggest that the CIC algorithm will be a useful addition to existing cloud classification tools but that further analyses of nadir radiance observations spanning the infrared and sampling a wider range of atmospheric and cloud conditions are required to fully probe its capabilities. This will be realised with the launch of the Far-infrared Outgoing Radiation Understanding and Monitoring (FORUM) mission, ESA's 9th Earth Explorer

    Ethics, space, and somatic sensibilities: comparing relationships between scientific researchers and their human and animal experimental subjects

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    Drawing on geographies of affect and nature-society relations, we propose a radical rethinking of how scientists, social scientists, and regulatory agencies conceptualise human and animal participants in scientif ic research. The scientific rationale for using animal bodies to simulate what could be done in human bodies emphasises shared somatic capacities that generate comparable responses to clinical interventions. At the same time, regulatory guidelines and care practices stress the differences between human and animal subjects. In this paper we consider the implications of this differentiation between human and animal bodies in ethical and welfare protocols and practices. We show how the bioethical debates around the use of human subjects tend to focus on issues of consent and language, while recent work in animal welfare reflects an increasing focus on the affectual dimensions of ethical practice. We argue that this attention to the more-than-representational dimensions of ethics and welfare might be equally important for human subjects. We assert that paying attention to these somatic sensibilities can offer insights into how experimental environments can both facilitate and restrict the development of more care-full and response-able relations between researchers and their experimental subjects. <br/

    Subharmonic bifurcation cascade of pattern oscillations caused by winding number increasing entrainment

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    Convection structures in binary fluid mixtures are investigated for positive Soret coupling in the driving regime where solutal and thermal contributions to the buoyancy forces compete. Bifurcation properties of stable and unstable stationary square, roll, and crossroll (CR) structures and the oscillatory competition between rolls and squares are determined numerically as a function of fluid parameters. A novel type of subharmonic bifurcation cascade (SC) where the oscillation period grows in integer steps as n(2π)/(ω)n (2\pi)/(\omega) is found and elucidated to be an entrainment process.Comment: 7 pages, 4 figure
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