Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering

Cultured cell-derived extracellular matrices (ECM)-based biomaterials exploit the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, standard cell culture conditions are far from ideal given the fact that the diluted microenvironment does not favor the production of ECM components, a circumstance particularly relevant for collagen. An incomplete conversion of procollagen by C-proteinase/bone morphogenetic protein 1 (BMP1) has been proposed to severely limit in vitro collagen deposition. BMP1 also catalyzes the proteolytic activation of the precursor of the collagen cross-linking enzyme, lysyl oxidase (LOX) to yield the active form, suggesting a deficit in cross-linking activity under standard conditions. We hypothesized that the implementation of fibroblast cultures with LOX and BMP1 may be an effective way to increase collagen deposition. To test it, we have generated stable cell lines overexpressing LOX and BMP1 and studied the effect of supernatants enriched in LOX and BMP1 on collagen synthesis and deposition from fibroblasts. Herein, we demonstrate that the supplementation with LOX and BMP1 strongly increased the deposition of collagen onto the insoluble matrix at the expense of the soluble fraction in the extracellular medium. Using decellularization protocols, we also show that fibroblast-derived matrices regulate adipogenic and osteogenic differentiation of human mesenchymal stem cells (MSC), and this effect was modulated by LOX/BMP1. Collectively, these data demonstrate that we have developed a convenient protocol to enhance the capacity of in vitro cell cultures to deposit collagen in the ECM, representing this approach a promising technology for application in tissue engineeringTis work was supported by grants from Ministerio de Economía y Competitividad (Plan Nacional de I+D+I: SAF2012-34916, and SAF2015-65679-R to F.R-P

Faint emission lines in the Galactic H II regions M16, M20 and NGC 3603

We present deep echelle spectrophotometry of the Galactic {\hii} regions M16, M20 and NGC 3603. The data have been taken with the Very Large Telescope Ultraviolet-Visual Echelle Spectrograph in the 3100 to 10400 \AA range. We have detected more than 200 emission lines in each region. Physical conditions have been derived using different continuum and line intensity ratios. We have derived He$^{+}$, C$^{++}$ and O$^{++}$ abundances from pure recombination lines as well as abundances from collisionally excited lines for a large number of ions of different elements. We have obtained consistent estimations of the temperature fluctuation parameter, {\ts}, using different methods. We also report the detection of deuterium Balmer lines up to D$\delta$ (M16) and to D$\gamma$ (M20) in the blue wings of the hydrogen lines, which excitation mechanism seems to be continuum fluorescence. The temperature fluctuations paradigm agree with the results obtained from optical CELs and the more uncertain ones from far IR fine structure CELs in NGC 3603, although, more observations covering the same volume of the nebula are necessary to obtain solid conclusions.Comment: 22 pages, 13 Tables, 7 Figures. Accepted for publication by MNRA

Spontaneous formation of vector vortex beams in vertical-cavity surface-emitting lasers with feedback

The spontaneous emergence of vector vortex beams with nonuniform polarization distribution is reported in a vertical-cavity surface-emitting laser (VCSEL) with frequency-selective feedback. Antivortices with a hyperbolic polarization structure and radially polarized vortices are demonstrated. They exist close to and partially coexist with vortices with uniform and nonuniform polarization distributions characterized by four domains of pairwise orthogonal polarization. The spontaneous formation of these nontrivial structures in a simple, nearly isotropic VCSEL system is remarkable and the vector vortices are argued to have solitonlike properties

Insights into the Carbon chemistry of Mon R2

Aiming to learn about the chemistry of the dense PDR around the ultracompact (UC) HII region in Mon R2, we have observed a series of mm-wavelength transitions of C3H2 and C2H. In addition, we have traced the distribution of other molecules, such as H13CO+, SiO, HCO, and HC3N. These data, together with the reactive ions recently detected, have been considered to determine the physical conditions and to model the PDR chemistry. We then identified two kind of molecules. The first group, formed by the reactive ions (CO+, HOC+) and small hydrocarbons (C2H, C3H2), traces the surface layers of the PDR and is presumably exposed to a high UV field (hence we called it as "high UV", or HUV). HUV species is expected to dominate for visual absorptions 2 < Av < 5 mag. A second group (less exposed to the UV field, and hence called "low UV", or LUV) includes HCO and SiO, and is mainly present at the edges of the PDR (Av > 5 mag). While the abundances of the HUV molecules can be explained by gas phase models, this is not the case for the studied LUV ones. Although some efficient gas-phase reactions might be lacking, grain chemistry sounds like a probable mechanism able to explain the observed enhancement of HCO and SiO. Within this scenario, the interaction of UV photons with grains produces an important effect on the molecular gas chemistry and constitutes the first evidence of an ionization front created by the UC HII region carving its host molecular cloud. The physical conditions and kinematics of the gas layer which surrounds the UC HII region were derived from the HUV molecules. Molecular hydrogen densities > 4 10^6 cm^(-3) are required to reproduce the observations. Such high densities suggest that the HII region could be pressure-confined by the surrounding high density molecular gas.Comment: 32 pages, 8 figures. Accepted by Astrophysical Journa