121 research outputs found
Association of Primary Varicose Veins with Dysregulated Vein Wall Apoptosis
BACKGROUND: Disordered programmed cell death may play a role in the development of superficial venous incompetence. We have determined the number of cells in apoptosis, and the mediators regulating the intrinsic and extrinsic pathways in specimens of varicose vein. METHODS: Venous segments were obtained from 46 patients undergoing surgical treatment for primary varicose veins. Controls samples were obtained from 20 patients undergoing distal arterial bypass grafting surgery. Segments of the distal and proximal saphenous trunk as well as tributaries were studied. Cell apoptoses and mediators of the mitochondrial and trans membrane pathway were evaluated with peroxidase in situ apoptosis detection, Bax and Fas detection, caspase-9 and 8 detection in the medial layer. RESULTS: Disorganised histological architecture was observed in varicose veins. Primary varicose veins also contained fewer peroxidase in situ-positive cells than control veins (2.6% S.D. 0.2% versus 12% S.D. 0.93%, P=.0001, Mann-Whitney u test), fewer Bax positive cells (2.1.% S.D. 0.3% versus 13% S.D. 0.9%, P=.0001) and fewer Caspase 9 positive cells (3.2% S.D. 1% versus 12% S.D. 1.3%, P=.0001). Similar findings were observed in saphenous trunk, main tributaries and accessory veins. In patients with recurrent varicose veins in whom the saphenous trunk had been preserved showed similar findings to primary varicose veins. Residual varicose veins contained fewer peroxidase in situ-positive cells than healthy veins (3.2% S.D. 0.6% versus 11% S.D. 2%, P=.0001), fewer Bax positive cells (2.2% S.D. 0.3% versus 12% S.D. 0.7%, P=.0001) and fewer Caspase 9 positive cells (2.6% S.D. 0.6% versus 12% S.D. 1%, P=.0001). Immunohistochemical detection for Fas and caspase 8 remained equal was the same in the varicose vein and control groups. CONCLUSION: Apoptosis is down regulated in the medial layer of varicose veins. This dysregulation is attributable to a disorder of the intrinsic pathway and involves the great saphenous vein trunk, major tributaries and accessory veins. This process may be among the causes of primary varicose veins
Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation
Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neuropixels recordings show that both sensory stimuli and motor initiation are represented by short-latency simple spikes. Optogenetic manipulation of short-latency simple spikes abolishes or delays motor initiation in a rate-dependent manner, indicating a role in motor initiation and its timing. Two-photon calcium imaging reveals task-related coherence of complex spikes organized into conserved alternating parasagittal stripes. The coherence of sensory-evoked complex spikes increases with learning and correlates with enhanced temporal precision of motor initiation. These results suggest that both simple spikes and complex spikes govern sensory-driven motor initiation: simple spikes modulate its latency, and complex spikes refine its temporal precision, providing specific cellular substrates for cerebellar sensorimotor control
The solar photospheric abundance of carbon.Analysis of atomic carbon lines with the CO5BOLD solar model
The use of hydrodynamical simulations, the selection of atomic data, and the
computation of deviations from local thermodynamical equilibrium for the
analysis of the solar spectra have implied a downward revision of the solar
metallicity. We are in the process of using the latest simulations computed
with the CO5BOLD code to reassess the solar chemical composition. We determine
the solar photospheric carbon abundance by using a radiation-hydrodynamical
CO5BOLD model, and compute the departures from local thermodynamical
equilibrium by using the Kiel code. We measure equivalent widths of atomic CI
lines on high resolution, high signal-to-noise ratio solar atlases. Deviations
from local thermodynamic equilibrium are computed in 1D with the Kiel code. Our
recommended value for the solar carbon abundance, relies on 98 independent
measurements of observed lines and is A(C)=8.50+-0.06, the quoted error is the
sum of statistical and systematic error. Combined with our recent results for
the solar oxygen and nitrogen abundances this implies a solar metallicity of
Z=0.0154 and Z/X=0.0211. Our analysis implies a solar carbon abundance which is
about 0.1 dex higher than what was found in previous analysis based on
different 3D hydrodynamical computations. The difference is partly driven by
our equivalent width measurements (we measure, on average, larger equivalent
widths with respect to the other work based on a 3D model), in part it is
likely due to the different properties of the hydrodynamical simulations and
the spectrum synthesis code. The solar metallicity we obtain from the CO5BOLD
analyses is in slightly better agreement with the constraints of
helioseismology than the previous 3D abundance results. (Abridged)Comment: Astronomy and Astrophysics, accepte
Spectroscopy of Blue Stragglers and Turnoff Stars in M67 (NGC 2682)
We have analyzed high-resolution spectra of relatively cool blue stragglers
and main sequence turnoff stars in the old open cluster M67 (NGC 2682). We
attempt to identify blue stragglers whose spectra are least contaminated by
binary effects (contamination by a binary companion or absorption by
circumstellar material). These ``best'' stragglers have metallicities ([Fe/H] =
-0.05) and abundance ratios of the blue stragglers are not significantly
different from those of the turnoff stars. Based on arguments from
hydrodynamical models of stellar collisions, we assert that the current upper
limits for the lithium abundances of all blue stragglers observed in M67 (by us
and others) are consistent with no mixing during the formation process,
assuming pre-main sequence and main sequence depletion patterns observed for
M67 main sequence stars. We discuss composition signatures that could more
definitively distinguish between blue straggler formation mechanisms in open
cluster stars.
We confirm the spectroscopic detection of a binary companion to the straggler
S 1082. From our spectra, we measure a projected rotational speed of 90+/-20
km/sec for the secondary, and find that its radial velocity varies with a
peak-to-peak amplitude of ~ 25 km/sec. Because the radial velocities do not
vary with a period corresponding to the partial eclipses in the system, we
believe this system is currently undergoing mass transfer. In addition we
present evidence that S 984 is a true blue straggler (and not an unresolved
pair). If this can be proven, our detection of lithium may indicate a
collisional origin.Comment: 20 pages, 4 figures, to appear in October 2000 A
Echelle long-slit optical spectroscopy of evolved stars
We present echelle long-slit optical spectra of a sample of objects evolving
off the AGB, most of them in the pre-planetary nebula (pPN) phase, obtained
with the ESI and MIKE spectrographs at Keck-II and Magellan-I, respectively.
The total wavelength range covered with ESI (MIKE) is ~3900 to 10900 A (~3600
to 7200A). In this paper, we focus our analysis mainly on the Halpha profiles.
Prominent Halpha emission is detected in half of the objects, most of which
show broad Halpha wings (up to ~4000 km/s). In the majority of the
Halpha-emission sources, fast, post-AGB winds are revealed by P-Cygni profiles.
In ~37% of the objects Halpha is observed in absorption. In almost all cases,
the absorption profile is partially filled with emission, leading to complex,
structured profiles that are interpreted as an indication of incipient post-AGB
mass-loss. All sources in which Halpha is seen mainly in absorption have F-G
type central stars, whereas sources with intense Halpha emission span a larger
range of spectral types from O to G. Shocks may be an important excitation
agent of the close stellar surroundings for objects with late type central
stars. Sources with pure emission or P Cygni Halpha profiles have larger J-K
color excess than objects with Halpha mainly in absorption, which suggests the
presence of warm dust near the star in the former. The two classes of profile
sources also segregate in the IRAS color-color diagram in a way that intense
Halpha-emitters have dust grains with a larger range of temperatures.
(abridged)Comment: 68 pages, 14 figures, accepted for publication in ApJS (abstract
abridged
V2324Cyg - an F-type star with fast wind
For the first time high-resolution optical spectroscopy of the variable star
V2324Cyg associated with the IR-source IRAS20572+4919 is made. More than 200
absorption features (mostly FeII, TiII, CrII, YII, BaII, and YII) are
identified within the wavelength interval 4549-7880AA. The spectral type and
rotation velocity of the star are found to be F0III and Vsini=69km/s,
respectively. HI and NaID lines have complex PCyg-type profiles with an
emission component. Neither systematic trend of radial velocity Vr with line
depth Ro nor temporal variability of Vr have been found. We determined the
average heliocentric radial velocity Vr=-16.8\pm 0.6km/s. The radial velocities
inferred from the cores of the absorption components of the H and NaI
wind lines vary from -140 to -225km/s (and the expansion velocities of the
corresponding layers, from about 120 to 210km/s). The maximum expansion
velocity is found for the blue component of the split H absorption:
450km/s for December 12, 1995. The model atmospheres method is used to
determine the star's parameters: Teff=7500K, log g=2.0, =6.0km/s, and
metallicity, which is equal to the solar value. The main peculiarity of the
chemical abundances pattern is the overabundance of lithium and sodium. The
results cast some doubt on the classification of V2324Cyg as a post-AGB star.Comment: 28 pages, 6 figure
Immunohistochemical localization of renin in the human kidney
By using an antiserum to purified human renal renin, renin was localized immunocytochemically in the human kidney under normal and various pathological conditions by the unlabeled antibody enzyme light microscope: (LM) and protein A-gold colloid electron microscope (EM) procedures. In the normal kidney, renin was confined to the epithelioid cells of the afferent arteriole of the juxtaglomerular apparatus (JGA). These cells were small and few, and always in the immediate neighborhood of the glomerulus. Fine structural analysis showed renin only in the secretion granules of the epitheloid cells. All granules within a given cell were stained with comparable intensity. In cases of renal artery stenosis (ischemic kidney) and of Bartter's syndrome, renin-positive epithelioid cells were larger, showed increased staining intensity, and were often found along the afferent arteriole at some distance from the glomerulus. Again, by electron microscopic observation, renin was seen only in secretion granules of epithelioid cells. In all of the above pathologic cases, plasma renin activity was very high. However, in the other nephropathies studied, renin staining in the kidney resembled that seen in normal kidneys, even when levels of plasma renin activity were quite high
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