382 research outputs found
Glitter-like iridescence within the bacteroidetes especially Cellulophaga spp.: optical properties and correlation with gliding motility.
This is the final version of the article. Available from the publisher via the DOI in this record.Iridescence results from structures that generate color. Iridescence of bacterial colonies has recently been described and illustrated. The glitter-like iridescence class, created especially for a few strains of Cellulophaga lytica, exhibits an intense iridescence under direct illumination. Such color appearance effects were previously associated with other bacteria from the Bacteroidetes phylum, but without clear elucidation and illustration. To this end, we compared various bacterial strains to which the iridescent trait was attributed. All Cellulophaga species and additional Bacteroidetes strains from marine and terrestrial environments were investigated. A selection of bacteria, mostly marine in origin, were found to be iridescent. Although a common pattern of reflected wavelengths was recorded for the species investigated, optical spectroscopy and physical measurements revealed a range of different glitter-like iridescence intensity and color profiles. Importantly, gliding motility was found to be a common feature of all iridescent colonies. Dynamic analyses of "glitter" formation at the edges of C. lytica colonies showed that iridescence was correlated with layer superposition. Both gliding motility, and unknown cell-to-cell communication processes, may be required for the establishment, in time and space, of the necessary periodic structures responsible for the iridescent appearance of Bacteroidetes.PV acknowledges the support of AFOSR grant FA9550-10-1-0020. BK was a PhD student with a grant from the Ministe`re de la recherche et de
l’enseignement supe´rieur. ER acknowledges the support of CNRS grant AIR75515 (‘‘Bacte´ridescence’’ project). The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the manuscript
Stretch-Activated Piezo1 Channel in Endothelial Cells Relaxes Mouse Intrapulmonary Arteries
In intrapulmonary artery (IPA), endothelial cells (EC) respond to mechanical stimuli by releasing vasoactive factors to set the vascular tone. Piezo1, a stretch-activated calcium permeable channel is a sensor of mechanical stress in EC. The present study was undertaken to investigate the implication of Piezo1 in the endothelium-dependent regulation of IPA tone and its potential involvement in pulmonary hypertension, the main disease of this circulation. IPA tone was quantified by means of a myograph in control Piezo1+/+ mouse and in mouse lacking endothelial Piezo1 (EC-Piezo1-/-). Endothelial intracellular calcium concentration ([Ca2+]i) and nitric oxide (NO) production were measured, in mouse or human EC, with fluo-4 and DAF-fm probes, respectively. Immunofluorescence labeling and patch-clamp experiments revealed the presence of Piezo1 channels in EC. Yoda1, a Piezo1 agonist, induced an endothelium-dependent relaxation that was significantly reduced in pulmonary arteries in EC-Piezo1-/- compared to Piezo1+/+ mouse. Yoda1 as well as mechanical stimulation (by osmotic stress) increased [Ca2+]i in mouse or human EC. Consequently, both stimuli increased the production of NO. NO and [Ca2+]i increases were reduced in EC from Piezo1-/- mouse or in the presence of Piezo1 inhibitors. Furthermore, deletion of Piezo1 increased alpha-adrenergic mediated contraction. Finally, in chronically hypoxic mice, a model of pulmonary hypertension, Piezo1 still mediated arterial relaxation and deletion of this channel did not impair the development of the disease. The present study thus demonstrates that endothelial Piezo1 contributes to intrapulmonary vascular relaxation by controlling endothelial [Ca2+]i and NO production and that this effect is still present in pulmonary hypertension
Production of medium-mass neutron-rich nuclei in reactions induced by 136Xe projectiles at 1 A GeV on a beryllium target
Production cross sections of medium-mass neutron-rich nuclei obtained in the
fragmentation of 136Xe projectiles at 1 A GeV have been measured with the
FRagment Separator (FRS) at GSI. 125Pd was identified for the first time. The
measured cross sections are compared to 238U fission yields and model
calculations in order to determine the optimum reaction mechanism to extend the
limits of the chart of the nuclides around the r-process waiting point at N=82.Comment: 9 pages, 6 figure
Coincidence measurement of residues and light particles in the reaction 56Fe+p at 1 GeV per nucleon with SPALADIN
The spallation of Fe in collisions with hydrogen at 1 A GeV has been
studied in inverse kinematics with the large-aperture setup SPALADIN at GSI.
Coincidences of residues with low-center-of-mass kinetic energy light particles
and fragments have been measured allowing the decomposition of the total
reaction cross-section into the different possible de-excitation channels.
Detailed information on the evolution of these de-excitation channels with
excitation energy has also been obtained. The comparison of the data with
predictions of several de-excitation models coupled to the INCL4 intra-nuclear
cascade model shows that only GEMINI can reasonably account for the bulk of
collected results, indicating that in a light system with no compression and
little angular momentum, multifragmentation might not be necessary to explain
the data.Comment: 4 pages, 5 figures, revised version accepted in Phys. Rev. Let
Iron and Nickel spectral opacity calculations in conditions relevant for pulsating stellar envelopes and experiments
Seismology of stars is strongly developing. To address this question we have
formed an international collaboration OPAC to perform specific experimental
measurements, compare opacity calculations and improve the opacity calculations
in the stellar codes [1]. We consider the following opacity codes: SCO,
CASSANDRA, STA, OPAS, LEDCOP, OP, SCO-RCG. Their comparison has shown large
differences for Fe and Ni in equivalent conditions of envelopes of type II
supernova precursors, temperatures between 15 and 40 eV and densities of a few
mg/cm3 [2, 3, 4]. LEDCOP, OPAS, SCO-RCG structure codes and STA give similar
results and differ from OP ones for the lower temperatures and for spectral
interval values [3]. In this work we discuss the role of Configuration
Interaction (CI) and the influence of the number of used configurations. We
present and include in the opacity code comparisons new HULLAC-v9 calculations
[5, 6] that include full CI. To illustrate the importance of this effect we
compare different CI approximations (modes) available in HULLAC-v9 [7]. These
results are compared to previous predictions and to experimental data.
Differences with OP results are discussed.Comment: 4 pages, 3 figures, conference Inertial Fusion Sciences and
Applications, Bordeaux, 12th to 16th September 2011; EPJ web of Conferences
201
Measurement of residual nucleus cross sections and recoil energies in p + Fe collisions at 300, 500, 750, 1000 and 1500 MeV
The production of residual nuclei in p + Fe collisions has been measured at GSI on the FRS facility by means of the reverse kinematic techniques at 300, 500, 750, 1000 and 1500 MeV/A. The cross-sections larger than 0.01 mb of all isotopes with Z larger than 8 have been obtained. Velocity distributions were also measured. Comparisons to models describing spallation reactions and some empirical formulae often used in astrophysics are presented. These data are directly used to calculate impurety production and DPAs in a thin window as foreseen in spallation sources or accelerator-driven systems
Cross-sectional associations between air pollution and chronic bronchitis: an ESCAPE meta-analysis across five cohorts
BACKGROUND: This study aimed to assess associations of outdoor air pollution on prevalence of chronic bronchitis symptoms in adults in five cohort studies (Asthma-E3N, ECRHS, NSHD, SALIA, SAPALDIA) participating in the European Study of Cohorts for Air Pollution Effects (ESCAPE) project. METHODS: Annual average particulate matter (PM10, PM2.5, PMabsorbance, PMcoarse), NO2, nitrogen oxides (NOx) and road traffic measures modelled from ESCAPE measurement campaigns 2008-2011 were assigned to home address at most recent assessments (1998-2011). Symptoms examined were chronic bronchitis (cough and phlegm for ≥3 months of the year for ≥2 years), chronic cough (with/without phlegm) and chronic phlegm (with/without cough). Cohort-specific cross-sectional multivariable logistic regression analyses were conducted using common confounder sets (age, sex, smoking, interview season, education), followed by meta-analysis. RESULTS: 15 279 and 10 537 participants respectively were included in the main NO2 and PM analyses at assessments in 1998-2011. Overall, there were no statistically significant associations with any air pollutant or traffic exposure. Sensitivity analyses including in asthmatics only, females only or using back-extrapolated NO2 and PM10 for assessments in 1985-2002 (ECRHS, NSHD, SALIA, SAPALDIA) did not alter conclusions. In never-smokers, all associations were positive, but reached statistical significance only for chronic phlegm with PMcoarse OR 1.31 (1.05 to 1.64) per 5 µg/m(3) increase and PM10 with similar effect size. Sensitivity analyses of older cohorts showed increased risk of chronic cough with PM2.5abs (black carbon) exposures. CONCLUSIONS: Results do not show consistent associations between chronic bronchitis symptoms and current traffic-related air pollution in adult European populations
Spallation Residues in the Reaction 56Fe + p at 0.3, 0.5, 0.75, 1.0 and 1.5 A GeV
The spallation residues produced in the bombardment of 56}Fe at 1.5, 1.0,
0.75, 0.5 and 0.3 A GeV on a liquid-hydrogen target have been measured using
the reverse kinematics technique and the Fragment Separator at GSI (Darmstadt).
This technique has permitted the full identification in charge and mass of all
isotopes produced with cross-sections larger than 10^{-2} mb down to Z=8. Their
individual production cross-sections and recoil velocities at the five energies
are presented. Production cross-sections are compared to previously existing
data and to empirical parametric formulas, often used in cosmic-ray
astrophysics. The experimental data are also extensively compared to different
combinations of intra-nuclear cascade and de-excitation models. It is shown
that the yields of the lightest isotopes cannot be accounted for by standard
evaporation models. The GEMINI model, which includes an asymmetric fission
decay mode, gives an overall good agreement with the data. These experimental
data can be directly used for the estimation of composition modifications and
damages in materials containing iron in spallation sources. They are also
useful for improving high precision cosmic-ray measurements.Comment: Submited to Phys. Rev. C (10/2006
- …