The Isolation, Culture, and Cryopreservation of Human Rectus Sheath Fibroblasts

The vast majority of pelvic and intra-abdominal surgery is undertaken through at least one incision, through either the linea alba or the rectus sheath. These connective tissue layers are formed from the aponeuroses of the rectus muscles (anterior and posterior rectus sheath) and are vital for the structural integrity of the abdominal wall. Poor healing of these connective tissues after surgery can lead to significant morbidity for patients, who can develop unsightly and painful incisional hernias. Fibroblasts within the rectus sheath are responsible for laying down and remodeling collagen during the healing process after surgery. Despite their importance for this healing process, such cells have not been studied in vitro. In order to carry out such work, researchers must first be able to isolate these cells from human tissue and culture them successfully so they may be used for experimentation. This article provides an extensive and detailed protocol for the isolation, culture, cryopreservation, and thawing of human rectus sheath fibroblasts (RSFs). In our hands, this protocol develops confluent cultures of primary fibroblasts within 2 weeks, and sufficient cultures ready for freezing and storage after a further 2 to 4 weeks. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Collagenase digestion of human rectus sheath and isolation of RSFs Alternate Protocol: Collagenase digestion of human rectus sheath and isolation of RSFs, digestion in flask Support Protocol: Cryopreservation and thawing of human RSFs

First Results from the KMOS Lens-Amplified Spectroscopic Survey (KLASS): Kinematics of Lensed Galaxies at Cosmic Noon

We present the first results of the KMOS Lens-Amplified Spectroscopic Survey (KLASS), a new ESO Very Large Telescope (VLT) large program, doing multi-object integral field spectroscopy of galaxies gravitationally lensed behind seven galaxy clusters selected from the HST Grism Lens-Amplified Survey from Space (GLASS). Using the power of the cluster magnification we are able to reveal the kinematic structure of 25 galaxies at $0.7 \lesssim z \lesssim 2.3$, in four cluster fields, with stellar masses $8 \lesssim \log{(M_\star/M_\odot)} \lesssim 11$. This sample includes 5 sources at $z>1$ with lower stellar masses than in any previous kinematic IFU surveys. Our sample displays a diversity in kinematic structure over this mass and redshift range. The majority of our kinematically resolved sample is rotationally supported, but with a lower ratio of rotational velocity to velocity dispersion than in the local universe, indicating the fraction of dynamically hot disks changes with cosmic time. We find no galaxies with stellar mass $<3 \times 10^9 M_\odot$ in our sample display regular ordered rotation. Using the enhanced spatial resolution from lensing, we resolve a lower number of dispersion dominated systems compared to field surveys, competitive with findings from surveys using adaptive optics. We find that the KMOS IFUs recover emission line flux from HST grism-selected objects more faithfully than slit spectrographs. With artificial slits we estimate slit spectrographs miss on average 60% of the total flux of emission lines, which decreases rapidly if the emission line is spatially offset from the continuum.Comment: Accepted for publication in Ap

The story of supernova 'Refsdal' told by MUSE

We present MUSE observations in the core of the HFF galaxy cluster MACS J1149.5+2223, where the first magnified and spatially-resolved multiple images of SN 'Refsdal' at redshift 1.489 were detected. Thanks to a DDT program with the VLT and the extraordinary efficiency of MUSE, we measure 117 secure redshifts with just 4.8 hours of total integration time on a single target pointing. We spectroscopically confirm 68 galaxy cluster members, with redshift values ranging from 0.5272 to 0.5660, and 18 multiple images belonging to 7 background, lensed sources distributed in redshifts between 1.240 and 3.703. Starting from the combination of our catalog with those obtained from extensive spectroscopic and photometric campaigns using the HST, we select a sample of 300 (164 spectroscopic and 136 photometric) cluster members, within approximately 500 kpc from the BCG, and a set of 88 reliable multiple images associated to 10 different background source galaxies and 18 distinct knots in the spiral galaxy hosting SN 'Refsdal'. We exploit this valuable information to build 6 detailed strong lensing models, the best of which reproduces the observed positions of the multiple images with a rms offset of only 0.26". We use these models to quantify the statistical and systematic errors on the predicted values of magnification and time delay of the next emerging image of SN 'Refsdal'. We find that its peak luminosity should should occur between March and June 2016, and should be approximately 20% fainter than the dimmest (S4) of the previously detected images but above the detection limit of the planned HST/WFC3 follow-up. We present our two-dimensional reconstruction of the cluster mass density distribution and of the SN 'Refsdal' host galaxy surface brightness distribution. We outline the roadmap towards even better strong lensing models with a synergetic MUSE and HST effort.Comment: 21 pages, 9 figures, 6 tables; accepted for publication in the Astrophysical Journal - extra information on data analysis added, all model predictions and results unchange

How Efficient Is The Langacker-Pi Mechanism of Monopole Annihilation?

We investigate the dynamics of monopole annihilation by the Langacker-Pi mechanism. We find taht considerations of causality, flux-tube energetics and the friction from Aharonov-Bohm scatteering suggest that the monopole annihilation is most efficient if electromagnetism is spontaneously broken at the lowest temperature ($T_{em} \approx 10^6 GeV$) consistent with not having the monopoles dominate the energy density of the universe.Comment: 10 page

A highly-ionized region surrounding SN Refsdal revealed by MUSE

Supernova (SN) Refsdal is the first multiply-imaged, highly-magnified, and spatially-resolved SN ever observed. The SN exploded in a highly-magnified spiral galaxy at z=1.49 behind the Frontier Fields Cluster MACS1149, and provides a unique opportunity to study the environment of SNe at high z. We exploit the time delay between multiple images to determine the properties of the SN and its environment, before, during, and after the SN exploded. We use the integral-field spectrograph MUSE on the VLT to simultaneously target all observed and model-predicted positions of SN Refsdal. We find MgII emission at all positions of SN Refsdal, accompanied by weak FeII* emission at two positions. The measured ratios of [OII] to MgII emission of 10-20 indicate a high degree of ionization with low metallicity. Because the same high degree of ionization is found in all images, and our spatial resolution is too coarse to resolve the region of influence of SN Refsdal, we conclude that this high degree of ionization has been produced by previous SNe or a young and hot stellar population. We find no variability of the [OII] line over a period of 57 days. This suggests that there is no variation in the [OII] luminosity of the SN over this period, or that the SN has a small contribution to the integrated [OII] emission over the scale resolved by our observations.Comment: 5 pages, 4 figures, accepted for publication in A&

The influence of the Lande gg-factor in the classical general relativistic description of atomic and subatomic systems

We study the electromagnetic and gravitational fields of the proton and electron in terms of the Einstenian gravity via the introduction of an arbitrary Lande $g$-factor in the Kerr-Newman solution. We show that at length scales of the order of the reduced Compton wavelength, corrections from different values of the $g$-factor are not negligible and discuss the presence of general relativistic effects in highly ionized heavy atoms. On the other hand, since at the Compton-wavelength scale the gravitational field becomes spin dominated rather than mass dominated, we also point out the necessity of including angular momentum as a source of corrections to Newtonian gravity in the quantum description of gravity at this scale.Comment: 11 pages, 2 figure